Dntt (NM_001043228) Mouse Tagged ORF Clone
CAT#: MR221098
- TrueORF®
Dntt (Myc-DDK-tagged) - Mouse deoxynucleotidyltransferase, terminal (Dntt), transcript variant 2
ORF Plasmid: tGFP
"NM_001043228" in other vectors (4)
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CNY 5,130.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Tdt |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR221098 representing NM_001043228
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCACTGCAAGCAGTCCACCTGGGTCCTCGGAAGAAAAGACCCAGGCAGCTGGGCACCCCAGTGG CTTCCACGCCTTATGACATCCGATTTCGAGACTTGGTCCTCTTCATTTTGGAAAAGAAGATGGGAACAAC TCGAAGAGCCTTCCTCATGGAGCTGGCCCGAAGGAAAGGGTTCAGGGTGGAAAATGAGCTCAGTGATTCG GTCACCCACATTGTGGCAGAGAACAACTCAGGGTCAGACGTCCTGGAGTGGCTCCAGCTGCAGAACATCA AAGCCAGCTCCGAGCTCGAACTCTTGGACATCTCCTGGCTCATCGAATGCATGGGAGCTGGGAAGCCAGT GGAGATGATGGGGAGACATCAGCTTGTTGTGAATAGAAACTCCTCCCCGAGTCCTGTCCCAGGCTCCCAG AATGTTCCAGCACCTGCTGTGAAGAAGATCTCCCAATATGCTTGCCAGCGAAGAACCACATTAAACAATT ACAACCAACTATTCACGGATGCCCTTGATATCCTGGCTGAAAATGATGAGCTTAGAGAGAATGAAGGTTC TTGCCTGGCATTCATGCGAGCGTCCTCTGTACTGAAATCTCTGCCATTCCCCATCACCAGCATGAAAGAC ACAGAGGGGATTCCTTGCCTAGGGGACAAGGTGAAGAGTATCATAGAGGGAATTATTGAAGATGGAGAAA GTTCTGAAGCTAAAGCTGTGTTAAATGATGAGCGATATAAATCCTTCAAACTCTTTACCTCTGTGTTTGG TGTGGGACTGAAGACAGCTGAGAAATGGTTCAGGATGGGTTTCAGAACTCTCAGCAAAATACAATCAGAC AAAAGCCTGAGGTTTACACAAATGCAGAAAGCAGGATTCCTCTACTACGAAGACCTCGTTAGCTGTGTGA ACAGGCCAGAAGCCGAGGCTGTCAGCATGCTAGTTAAGGAGGCGGTTGTGACATTTCTTCCAGATGCCTT GGTCACCATGACTGGGGGGTTCCGCAGGGGTAAGATGACTGGACATGATGTAGACTTTCTAATTACCAGC CCAGAAGCCACAGAGGATGAAGAGCAGCAGCTCTTGCATAAAGTGACAGACTTTTGGAAGCAGCAGGGGT TGCTTTTGTACTGCGACATCTTAGAGTCAACCTTTGAAAAGTTCAAGCAGCCCAGCAGGAAGGTGGATGC TCTCGACCATTTCCAGAAATGCTTCCTGATTCTGAAGCTGGACCACGGGAGAGTGCACAGCGAGAAGAGC GGCCAGCAGGAAGGAAAGGGCTGGAAGGCCATCCGTGTAGATCTGGTCATGTGCCCCTATGATCGCCGGG CCTTCGCCCTGCTTGGATGGACCGGCTCCAGACAGTTTGAGAGAGACTTGCGGCGCTATGCCACACACGA GAGGAAGATGATGCTGGACAACCACGCCCTGTATGACAGGACTAAGAGGGTGTTTCTTGAAGCAGAAAGT GAAGAAGAGATCTTTGCCCATCTGGGACTGGACTACATTGAACCATGGGAAAGAAATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221098 representing NM_001043228
Red=Cloning site Green=Tags(s) MDPLQAVHLGPRKKRPRQLGTPVASTPYDIRFRDLVLFILEKKMGTTRRAFLMELARRKGFRVENELSDS VTHIVAENNSGSDVLEWLQLQNIKASSELELLDISWLIECMGAGKPVEMMGRHQLVVNRNSSPSPVPGSQ NVPAPAVKKISQYACQRRTTLNNYNQLFTDALDILAENDELRENEGSCLAFMRASSVLKSLPFPITSMKD TEGIPCLGDKVKSIIEGIIEDGESSEAKAVLNDERYKSFKLFTSVFGVGLKTAEKWFRMGFRTLSKIQSD KSLRFTQMQKAGFLYYEDLVSCVNRPEAEAVSMLVKEAVVTFLPDALVTMTGGFRRGKMTGHDVDFLITS PEATEDEEQQLLHKVTDFWKQQGLLLYCDILESTFEKFKQPSRKVDALDHFQKCFLILKLDHGRVHSEKS GQQEGKGWKAIRVDLVMCPYDRRAFALLGWTGSRQFERDLRRYATHERKMMLDNHALYDRTKRVFLEAES EEEIFAHLGLDYIEPWERNA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001043228 |
ORF Size | 1530 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001043228.1, NP_001036693.1 |
RefSeq Size | 2073 bp |
RefSeq ORF | 1533 bp |
Locus ID | 21673 |
UniProt ID | P09838 |
MW | 58.7 kDa |
Gene Summary | Isoform TDT-S: Transferase that catalyzes the nontemplated addition of nucleoside triphosphate to coding ends during V(D)J recombination (N addition). Involved in the generation of diversity in the antigen-binding region of immunoglobulin heavy and light chains and T-cell receptors during B-and T-cell development. Does not act on double-stranded DNA with blunt ends.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217150 | Dntt (untagged) - Mouse deoxynucleotidyltransferase, terminal (Dntt), transcript variant 2, (10ug) |
CNY 6,560.00 |
|
MG221098 | Dntt (tGFP-tagged) - Mouse deoxynucleotidyltransferase terminal (Dntt) transcript variant 2, (10ug) |
CNY 7,672.00 |
|
MR221098L3 | Lenti ORF clone of Dntt (Myc-DDK-tagged) - Mouse deoxynucleotidyltransferase, terminal (Dntt), transcript variant 2 |
CNY 7,030.00 |
|
MR221098L4 | Lenti ORF clone of Dntt (mGFP-tagged) - Mouse deoxynucleotidyltransferase, terminal (Dntt), transcript variant 2 |
CNY 7,030.00 |