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Nlrp12 (NM_001033431) Mouse Tagged ORF Clone
CAT＃: MR221409
- TrueORF®
Nlrp12 (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 12 (Nlrp12)
ORF Plasmid: tGFP
"NM_001033431" in other vectors (4)
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CNY 11,008.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Nalp12; PYPAF7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221409 representing NM_001033431
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGCCGTCTACAGCCAGGGATGGCCTCTATCGACTGTCTACCTACCTGGAAGAACTCGAGGCTGGGG AACTGAAGAAATTCAAATTATTCCTGGGGATTGCAGAGGACCTGAGCCAGGACAAAATTCCCTGGGGACG AATGGAGAAGGCTGGTCCTCTGGAAATGGCTCAGCTGATGGTGGCCCACATGGGGACAAGGGAGGCTTGG CTTCTGGCTCTCAGCACCTTTCAGAGGATTCACAGGAAGGACCTGTGGGAGCGAGGACAGGGAGAAGACC TGGTGAGGGTCACTCCAAATAATGGTCTATGCCTTTTTGAGAGCCAATCAGCGTGCCCTTTGGATGTCTC TCCCAATGCTCCAAGAAAAGATCTACAGACAACCTACAAAGACTATGTCCGAAGGAAATTCCAGCTAATG GAAGACCGCAATGCACGATTAGGCGAATGTGTGAACCTGAGCAATCGTTACACTCGGCTTCTCCTAGTAA AAGAACACTCAAATCCTATCTGGACACAGCAGAAATTTGTAGATGTAGAGTGGGAACGCTCCAGAACCAG GCGTCACCAGACTAGTCCTATCCAAATGGAGACCCTCTTTGAGCCAGACGAAGAACGCCCCGAGCCACCA CACACAGTGGTATTACAAGGGGCAGCGGGGATGGGGAAGTCCATGCTGGCCCACAAAGTGATGTTGGACT GGGCCGATGGGAGGCTCTTCCAAGGCCGGTTTGATTATGTCTTCTATATCAGCTGCAGGGAGTTGAATAG AAGCCACACCCAGTGCAGTGTACAAGACCTCATCTCCAGCTGCTGGCCGGAGCGTGGTATATCCCTCGAA GACCTCATGCAGGCTCCTGACCGTCTCCTATTCATCATTGATGGCTTCGATAAACTCCATCCTTCTTTCC ATGATGCTCAGGGTCCCTGGTGCCTCTGCTGGGAGGAGAAACAACCTACTGAAGTCCTCCTCGGAAGTCT GATTCGGAGGTTGCTTCTGCCCCAGGTCTCTCTGCTCATCACCACACGACCCTGTGCACTGGAGAAGCTG CACGGCTTGCTAGAACACCCCAGGCACGTGGAGATCCTGGGCTTCTCCGAGGAAGCTAGGAAGGAATATT TCTACAGATATTTCCACAACACTGGACAAGCAAGCCGGGTGTTAAGCTTCTTGATGGACTATGAGCCCCT CTTTACCATGTGTTTTGTTCCCATGGTGTCCTGGGTGGTCTGCACCTGCCTAAAGCAGCAGCTGGAAAGT GGGGAGCTTTTAAGACAAACACCTAGGACCACCACAGCTGTTTATATGTTCTACCTTCTGAGCCTGATGC AGCCCAAGCCAGGGACTCCAACCTTCAAAGTCCCAGCCAACCAGAGAGGCCTGGTCTCTCTGGCTGCAGA GGGCCTCTGGAATCAGAAGATTCTATTTGATGAACAGGATCTTGGGAAACACGGCCTAGATGGAGCAGAT GTGTCCACTTTCCTCAACGTGAACATATTCCAGAAGGGTATCAAATGTGAGAAATTCTACAGCTTCATCC ACCTGAGTTTCCAGGAATTCTTCGCAGCCATGTACTGTGCACTGAATGGCAGAGAGGCGGTGAGGAGAGC GCTGGCTGAGTATGGTTTTTCGGAAAGGAACTTCTTGGCCCTCACGGTCCACTTTCTGTTTGGCCTCCTC AACGAAGAGATGAGATGCTACCTTGAGAGGAATCTCGGCTGGAGCATCTCCCCTCAGGTGAAGGAGGAAG TGTTGGCATGGATCCAAAACAAGGCTGGGAGTGAAGGCTCCACCCTGCAGCATGGCTCCCTGGAGCTACT CAGCTGCTTGTATGAGGTCCAGGAGGAGGACTTCATCCAGCAGGCCCTGAGCCACTTTCAAGTGGTTGTA GTCAGAAGCATCTCAACAAAGATGGAGCACATGGTCTGCTCGTTTTGTGCGAGGTATTGCAGAAGTACAG AAGTGCTTCACTTGCATGGGAGTGCTTATAGTACAGGCATGGAGGACGACCCACCAGAACCTTCAGGAGT CCAGACTCAGTCCACATACTTACAGGAAAGGAACATGCTGCCTGATGTCTACAGTGCATACCTTTCAGCA GCTGTCTGTACCAACTCCAACCTGATCGAGCTGGCCTTATACCGAAATGCCTTGGGCAGCCAGGGTGTAA GGCTGCTCTGTCAAGGCCTCCGACATGCCAGCTGCAAGCTGCAGAACCTGAGGCTGAAGAGGTGTCAGAT CTCCGGATCAGCCTGCCAGGACCTCGCAGCCGCTGTCATCGCCAACAGGAATTTAATCAGGCTGGACCTC AGTGACAACAGCATTGGGGTGCCAGGCCTGGAGCTGCTCTGTGAGGGGCTGCAGCACCCCAGGTGTAGGC TGCAGATGATCCAGCTGAGGAAGTGTCTGTTGGAGGCTGCAGCTGGCCGATCCCTGGCTTCTGTTCTCAG CAACAACTCATATCTGGTAGAACTGGATCTGACAGGAAACCCCTTGGAAGATTCGGGGCTGAAGTTACTG TGTCAAGGGCTAAGGCACCCTGTCTGCAGGCTGCGTACCCTGTGGCTGAAGATCTGCCACCTTGGACAAG CTTCCTGCGAAGATCTGGCCTCTACTCTCAAAATGAACCAGAGCCTGCTGGAGCTGGACCTGGGTCTGAA TGATCTTGGAGATTCTGGGGTGCTTCTGCTGTGTGAAGGCCTCAGTCATCCAGATTGCAAACTCCAGACC CTTCGGTTGGGCATTTGCCGACTGGGCTCAGTCGCGTGTGTGGGGATCGCCAGTGTGCTCCAGGTCAACA CATGCCTCCAAGAGCTGGACCTGAGCTTCAATGACTTGGGAGACAGGGGCCTGCAGCTGCTGGGGGAAGG CCTGAGGCACCAGACCTGCAGACTCCAGAAGCTGTGGCTGGACAACTGCGGACTCACCTCCAAAGCATGT GAGGACCTTTCTTCTATCCTGGGAATCAGCCAGACCCTGCATGAGCTTTATTTGACCAATAATGCTCTGG GGGACACAGGTGTCTGTCTGCTGTGCAAGAGGCTGAGGCATCCAGGCTGCAAGCTTCGAGTCCTGTGGCT GTTTGGGATGGACCTGAATAAAAAGACTCACAGGAGGATGGCAGCACTTCGAGTCACAAAACCGTACCTG GATATTGGGTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221409 representing NM_001033431
Red=Cloning site Green=Tags(s) MLPSTARDGLYRLSTYLEELEAGELKKFKLFLGIAEDLSQDKIPWGRMEKAGPLEMAQLMVAHMGTREAW LLALSTFQRIHRKDLWERGQGEDLVRVTPNNGLCLFESQSACPLDVSPNAPRKDLQTTYKDYVRRKFQLM EDRNARLGECVNLSNRYTRLLLVKEHSNPIWTQQKFVDVEWERSRTRRHQTSPIQMETLFEPDEERPEPP HTVVLQGAAGMGKSMLAHKVMLDWADGRLFQGRFDYVFYISCRELNRSHTQCSVQDLISSCWPERGISLE DLMQAPDRLLFIIDGFDKLHPSFHDAQGPWCLCWEEKQPTEVLLGSLIRRLLLPQVSLLITTRPCALEKL HGLLEHPRHVEILGFSEEARKEYFYRYFHNTGQASRVLSFLMDYEPLFTMCFVPMVSWVVCTCLKQQLES GELLRQTPRTTTAVYMFYLLSLMQPKPGTPTFKVPANQRGLVSLAAEGLWNQKILFDEQDLGKHGLDGAD VSTFLNVNIFQKGIKCEKFYSFIHLSFQEFFAAMYCALNGREAVRRALAEYGFSERNFLALTVHFLFGLL NEEMRCYLERNLGWSISPQVKEEVLAWIQNKAGSEGSTLQHGSLELLSCLYEVQEEDFIQQALSHFQVVV VRSISTKMEHMVCSFCARYCRSTEVLHLHGSAYSTGMEDDPPEPSGVQTQSTYLQERNMLPDVYSAYLSA AVCTNSNLIELALYRNALGSQGVRLLCQGLRHASCKLQNLRLKRCQISGSACQDLAAAVIANRNLIRLDL SDNSIGVPGLELLCEGLQHPRCRLQMIQLRKCLLEAAAGRSLASVLSNNSYLVELDLTGNPLEDSGLKLL CQGLRHPVCRLRTLWLKICHLGQASCEDLASTLKMNQSLLELDLGLNDLGDSGVLLLCEGLSHPDCKLQT LRLGICRLGSVACVGIASVLQVNTCLQELDLSFNDLGDRGLQLLGEGLRHQTCRLQKLWLDNCGLTSKAC EDLSSILGISQTLHELYLTNNALGDTGVCLLCKRLRHPGCKLRVLWLFGMDLNKKTHRRMAALRVTKPYL DIGC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033431 |
| ORF Size | 3162 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033431.1, NP_001028603.1 |
| RefSeq Size | 4329 bp |
| RefSeq ORF | 3165 bp |
| Locus ID | 378425 |
| UniProt ID | E9Q5R7 |
| MW | 119.8 kDa |
| Gene Summary | Plays an essential role as an potent mitigator of inflammation (PubMed:26521018, PubMed:30559449). Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK activation pathways (PubMed:30559449). Functions as a negative regulator of NOD2 by targeting it to degradation via the proteasome pathway (PubMed:30559449). In turn, promotes bacterial tolerance (PubMed:30559449). Inhibits also the DDX58-mediated immune signaling against RNA viruses by reducing the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked DDX58 activation but enhancing the E3 ubiquitin ligase RNF125-mediated 'Lys-48'-linked DDX58 degradation (By similarity). Acts also as a negative regulator of inflammatory response to mitigate obesity and obesity-associated diseases in adipose tissue (PubMed:30212649).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223405 | Nlrp12 (untagged) - Mouse NLR family, pyrin domain containing 12 (Nlrp12), (10ug) |
CNY 13,590.00 |
|
| MG221409 | Nlrp12 (tGFP-tagged) - Mouse NLR family pyrin domain containing 12 (Nlrp12), (10ug) |
CNY 12,608.00 |
|
| MR221409L3 | Lenti ORF clone of Nlrp12 (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 12 (Nlrp12) |
CNY 12,450.00 |
|
| MR221409L4 | Lenti ORF clone of Nlrp12 (mGFP-tagged) - Mouse NLR family, pyrin domain containing 12 (Nlrp12) |
CNY 12,450.00 |
