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Adamts8 (NM_013906) Mouse Tagged ORF Clone
CAT＃: MR221476
- TrueORF®
Adamts8 (Myc-DDK-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 8 (Adamts8)
ORF Plasmid: tGFP
"NM_013906" in other vectors (4)
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CNY 6,632.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | METH-2; METH2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221476 representing NM_013906
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCGCGACCCCACCACCACCGGGTGGCCGCCCCTCCTGCTGCTGCTATTGCAGCTGCCGCCGCCGC CACTCGTCTGCGGCGCCCCGGCGGGGCCGGGAACCGGGGCGCAGGCCTCGGAGCTAGTGGTGCCCACGCG GTTGCCCGGCAGCGCGAGCGAGCTCGCCTTCCACCTGTCCGCCTTCGGCCAGGGCTTCGTGCTGCGCCTG GCGCCTGACGCCAGCTTCCTGGCGCCGGAATTCAAGATCGAGCGCCTCGGGGGCTCGAGCGCGGCGGCCG GGGGCGAGCCGGGACTGCGTGGCTGCTTCTTCTCTGGCACAGTGAATGGAGAACGGGAGTCGCTGGCGGC GATGAGCCTGTGTCGCGGGCTGAGCGGCTCGTTCTTGCTGGCAGGCGAGGAGTTCACCATCCAGCCACAG GGCGCTGGGGACTCCCTGGACCAGCCTCATCGCCTGCAGCGCTGGGGGCCGGGACAGCGCCGCGAAGACC CCGGGCTCGCTGCCGCCGAAGTTTTCCCCCTCCCTCAAGGACTGGAGTGGGAGGTGGAGATGGGTAATGG GCAGGGACAGGAGAGAAGTGACAACGAAGAGGACAGGAAGCAGGACAAGGAGGGGTTGCTCAAAGAGACA GAAGACTCCCGCAAAGTGCCACCACCCTTCGGATCCAAAACTAGAAGCAAGAGGTTTGTGTCCGAGGCTC GCTTCGTGGAAACACTTCTGGTGGCTGATGCGTCCATGGCTGCCTTCTATGGGACCGACCTGCAGAACCA CATCCTCACGGTGATGTCAATGGCAGCCCGAATCTACAAGCACCCGAGCATCAGGAACTCCGTCAACCTT GTGGTGGTGAAAGTGCTAATAGTGGAAGAGGAAGGATGGGGCCCGGAGGTGTCGGACAACGGGGGGCTCA CACTGCGCAACTTCTGCAGCTGGCAACGGCGTTTCAACAAGCCCAGTGACCGCCACCCGGAGCACTATGA CACTGCCATCTTGTTCACCAGACAGAACTTCTGTGGGAAGGGAGAGCAGTGTGACACCCTGGGGATGGCA GACGTTGGCACCATCTGTGACCCCGACAAGAGCTGCTCAGTGATCAAGGATGAGGGACTGCAGGCAGCCT ACACCCTGGCCCATGAGCTAGGGCACGTTCTCAGCATGCCCCATGATGATTCTAAGCCCTGTGTGAGATT GTTTGGGCCCATGGGCAAGTACCACATGATGGCGCCATTCTTCATCCACGTGAACAAGACGCTGCCCTGG TCTCCCTGCAGTGCTGTCTACCTCACAGAGCTCCTGGATGATGGTCACGGAGATTGTCTTCTGGATGCCC CCACCTCGGTTCTGCCCCTCCCCACAGGCCTCCCGGGCCACAGCACCCTCTACGAGCTGGACCAGCAGTG CAAGCAGATCTTTGGGCCTGATTTCCGACACTGCCCCAACACCTCTGTGGAGGACATCTGTGTCCAGCTC TGGTGCCGTCATCGGGATAGTGATGAGCCCATTTGCCACACAAAGAATGGTAGCCTGCTCTGGGCTGATG GTACACCCTGTGGCCCTGGGCACCTGTGCCTGGATGGTAGCTGTGTACTCAAGGAGGATGTGGAGAATCC CAAGGCTGTGGTAGATGGAGACTGGGGTCCCTGGAGACCCTGGGGACAATGTTCTCGCACCTGTGGTGGA GGGATACAATTCTCGAACCGTGAATGTGATAATCCAATGCCTCAGAATGGAGGAAGATTTTGCCTGGGTG AAAGAGTCAAGTACCAATCATGCAACACAGAGGAATGTCCACCAAACGGAAAAAGCTTCCGGGAGCAGCA GTGTGAGAAATATAATGCCTACAACCACACTGACCTGGATGGGAATTTCCTGCAGTGGGTCCCCAAGTAT TCAGGAGTGTCCCCCCGAGACCGATGCAAGCTGTTTTGCAGAGCCCGTGGGAGGAGTGAGTTCAAAGTGT TTGAAGCTAAGGTGATCGATGGCACTCTGTGTGGACCGGATACTCTGTCCATCTGCGTCCGGGGGCAATG TGTTAAGGCTGGCTGTGACCATGTGGTGAACTCACCTAAGAAGCTGGACAAATGTGGGGTGTGTGGGGGC AAAGGCACTGCCTGTAGGAAGATCTCCGGTTCTTTCACCCCCTTCAGTTATGGCTACAATGACATTGTCA CCATCCCAGCTGGTGCCACAAACATTGATGTGAAACAGCGGAGTCACCCAGGGGTCAGGAACGACGGCAG CTACCTGGCGCTGAAGACAGCCAATGGGCAGTACCTGCTCAATGGTAACCTGGCCATCTCTGCCATAGAG CAAGACATCTTGGTGAAGGGGACCATCCTGAAGTACAGTGGCTCCATGGCTACCCTGGAGCGGCTGCAGA GCTTCCAGGCCCTGCCTGAGCCTCTTACAGTACAGCTCCTGACTGTGTCTGGTGAGGTCTTCCCTCCAAA AGTCAGATATACCTTCTTTGTCCCCAATGACATGGACTTCAGCGTGCAGAATAGCAAGGAAAGAGCAACC ACCAACATCATTCAGTCACTGCCCTCTGCGGAGTGGGTTCTGGGAGACTGGTCTGAATGTCCGAGCACGT GCAGAGGTAGCTGGCAGCGGCGGACTGTGGAATGCAGGGACCCCTCAGGTCAGGCCTCTGACACCTGTGA TGAGGCTCTGAAACCTGAGGATGCCAAGCCCTGTGGAAGCCAGCCGTGTCCCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221476 representing NM_013906
Red=Cloning site Green=Tags(s) MLRDPTTTGWPPLLLLLLQLPPPPLVCGAPAGPGTGAQASELVVPTRLPGSASELAFHLSAFGQGFVLRL APDASFLAPEFKIERLGGSSAAAGGEPGLRGCFFSGTVNGERESLAAMSLCRGLSGSFLLAGEEFTIQPQ GAGDSLDQPHRLQRWGPGQRREDPGLAAAEVFPLPQGLEWEVEMGNGQGQERSDNEEDRKQDKEGLLKET EDSRKVPPPFGSKTRSKRFVSEARFVETLLVADASMAAFYGTDLQNHILTVMSMAARIYKHPSIRNSVNL VVVKVLIVEEEGWGPEVSDNGGLTLRNFCSWQRRFNKPSDRHPEHYDTAILFTRQNFCGKGEQCDTLGMA DVGTICDPDKSCSVIKDEGLQAAYTLAHELGHVLSMPHDDSKPCVRLFGPMGKYHMMAPFFIHVNKTLPW SPCSAVYLTELLDDGHGDCLLDAPTSVLPLPTGLPGHSTLYELDQQCKQIFGPDFRHCPNTSVEDICVQL WCRHRDSDEPICHTKNGSLLWADGTPCGPGHLCLDGSCVLKEDVENPKAVVDGDWGPWRPWGQCSRTCGG GIQFSNRECDNPMPQNGGRFCLGERVKYQSCNTEECPPNGKSFREQQCEKYNAYNHTDLDGNFLQWVPKY SGVSPRDRCKLFCRARGRSEFKVFEAKVIDGTLCGPDTLSICVRGQCVKAGCDHVVNSPKKLDKCGVCGG KGTACRKISGSFTPFSYGYNDIVTIPAGATNIDVKQRSHPGVRNDGSYLALKTANGQYLLNGNLAISAIE QDILVKGTILKYSGSMATLERLQSFQALPEPLTVQLLTVSGEVFPPKVRYTFFVPNDMDFSVQNSKERAT TNIIQSLPSAEWVLGDWSECPSTCRGSWQRRTVECRDPSGQASDTCDEALKPEDAKPCGSQPCPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013906 |
| ORF Size | 2715 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013906.3, NP_038934.2 |
| RefSeq Size | 3632 bp |
| RefSeq ORF | 2718 bp |
| Locus ID | 30806 |
| MW | 99.4 kDa |
| Gene Summary | This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. This gene is expressed in mouse lung, heart and macrophage-rich areas of atherosclerotic plaques. The encoded preproprotein undergoes proteolytic processing to generate an active, zinc-dependent aggrecanase enzyme. This gene is located adjacent to a related ADAMTS gene on chromosome 9. [provided by RefSeq, May 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222526 | Adamts8 (untagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 8 (Adamts8), (10ug) |
CNY 11,690.00 |
|
| MG221476 | Adamts8 (tGFP-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif 8 (Adamts8), (10ug) |
CNY 7,890.00 |
|
| MR221476L3 | Lenti ORF clone of Adamts8 (Myc-DDK-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 8 (Adamts8) |
CNY 9,030.00 |
|
| MR221476L4 | Lenti ORF clone of Adamts8 (mGFP-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 8 (Adamts8) |
CNY 9,030.00 |
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