Adamtsl3 (NM_001190374) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9230119C12Rik; C130057K09; mKIAA1233
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR221800 representing NM_001190374 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGAGGGAGACTCCTATGATATTGGACCCCATGGCTTCCTGGACAGGATCCTGGTGGCTGCTGA CACGAATGATCTTTACGGTCTCTGGTCTTCTGCAGGCTACAGCAGAGAAGTCTCCTGGAGCCTACTTCCT TCCTGAGTTTGCGCTCTCTCCTCAGGGAAGCTTCCTAGAAGACACCACAGGGGAGCAGTTCCTCACATAC CGCTATGATGACCAGACTTCAAGAAATGCTCATTCAGAAGAAGACAAGGATGGCAACTGGGACGCCTGGG GAGACTGGAGTGACTGCTCCCGGACCTGTGGCGGAGGAGCCTCCTATTCTTTGCGGAGATGTCTGACTGG AAGGAACTGCGAAGGACAGAACATCAGATACAAGACTTGCAGCAATCACGACTGCCCAGCAGATGCAGAA GACTTCAGAGCCCAGCAGTGCTCAGCCTACAATGACGTGCAGTACCAGGGTCATTATTATGAATGGATTC CACTATACAATGACCCTGCTGCCCCATGTGCACTCAAGTGTCACGCTCGGGGACAGAGTTTGGTGGTAGA GCTGGCACCCAAAGTGTTGGATGGAACTCGCTGCAATGCTGACTCTCTGGATATGTGCATAAGTGGCATC TGCCAGATTGTGGGCTGTGATAGACAGCTGGGAAGTAATGCCAAGGAGGACAATTGTGGCGTCTGTGCTG GGGACGGCTCCACCTGCAGGCTGGTGCGGGGTCAGACCAAGATGCACCTGTCTCCTGAGAAGAAGGAAGA AAATGTGATTGCCGTTCCCTTGGGAAGTCGAAGTGTGAGGATTACGGTGAAAGGACCTGCCCTGATCTTT ATTGAGTCCAAAACACTTCAAGGAAGCAGAGGAGAGCACAGCTTCAACAGTCCTGGGGTTTTCACTGTAG AAAACACGACGATTGAGTTTCAGAAGGGTGCGGACAGACAAACCTTCAAGATTGCGGGACCCCTGATGGC TGATTTCATCTTCAAGACCAGGTACACCGTGGCCAAAGGCAGCGTGGTCCAGTTCTTCTTTTACCAGCCC ATCAGCCATCAGTGGAGACAGACTGACTTCTTTCCCTGCACCGTGACATGTGGAGGAGGTTATCAGCTCA ACTCTGCTGAGTGCATGGACATCCGCTTGAAAAGGGTAGTGCCTGACCACTATTGCCATTACTACCCAGA AAATGTGAAGCCTAAACCAAAGCTGAAGGAATGCAGTATGGATCCTTGCCCATCCAGTGATGGATTTAAA GAGATTATGCCCTACGACCACTTCCAACCTCTCCCTCGCTGGGAACATAATCCCTGGACTGCATGTTCGG TGTCCTGTGGAGGAGGGATTCAGAGACGGAGTTTTGTGTGTGTGGAGGAATCTATGCATGCAGAGATTCT ACAAGTGGAAGAATGGAAGTGCATGTATGCACCCAAGCCCACGGTGATGCAGGCTTGCAACCTCTTTGAT TGCCCCAAGTGGGTTGCCATGGAGTGGTCTCAGTGCACAGTGACTTGTGGCCGAGGGTTGCGCTACCGAG TGGTTCTGTGTATCAACCATCGTGGGCAACATGTTGGAGGCTGCAACCCACAGATGAAGCTGCACATCAA AGAGGAGTGTGTCATCCCCATCCCGTGCTATAAGCCCAGAGAAAAAAGTCCAGTGGAAGCTAAATTACCT TGGCTGAAACAAGCACAAGAGCTAGAAGAGACCAGAATAGCCACAGAAGAACCAAAGTTCATCCCAGAGC CCTGGTCAGCCTGCAGCACCACGTGCGGGCCTGGCATTCAGGTTCGTGAGGTGCGGTGTCGGGTGCTCCT CACGTTCACACAGACGGAGGCTGAGCTCCCTGAGGAGGAGTGTGAAGGCCCCAAGCCACCCACTGACCGA CCCTGCCTCCGTCAAGCCTGTGACCAGAGCCCTGTGTCTCGAGAACTGGGTGGCCGTCTCCAAGAGAAGG ACAGTGAGATGACTTACGACTGGGAGTACGCTGGCTTTACCCCCTGCACTGCAACATGTTTGGGAGGCCA TCAAGAAGCCATTGCTGTGTGTTTACACACCCAGACTCATCAGACCGTCAATGACAGCCTGTGTGACACA GTTCACCGTCCTCCACCAATGAGTCAGGCCTGTAACATGGAGCCCTGCCCGCCCAGATGGCACATGGGCT CTTGGGGAGCCTGTTCAGCTACCTGTGGTGTTGGAATCCAGACACGAGATGTATACTGCCTCCATCCTGA GGAATTCCCAGCCCCTCCGGAGGAATGCAGAGAGGAAAAGCCGCATGCCTTACGAGCGTGCAATCAGTTT GATTGCCCTCCCAGCTGGCACATCGAAGAGTGGCAGCAGTGTTCCAGGACATGCGGTGGGGGAACTCAGA ACCGGAGAGTCACCTGTCGACAGCTGTTAACTGATGGCAGCTTTTTGAATTTGTCGGATGAGCTGTGCCA AGGACCCAAGGCATCGTCTCATAAGTCCTGTGCCAGGACAGACTGCCCCCCACAGCTGGCTGCCGGAGAC TGGTCGAAGTGCTCTGTCACCTGCGGGGTTGGATTCCAGAGAAGAAAGCAGGTGTGTCAAAAACTGACTG CCAAAGGCCGAAGAGTCTCCGTAGCTGAGACGCTGTGCAGGGACCTGCCAGGGCTCCCCCTCGTGAGACC ATGCCAGATGCCTATATGCAGCAAAGCGAAACTGGGAACGAAGACAAAACTTGGAGAGAGGGGCCCCCAG ATTCTTGGTGTCCGCAGAGTCTATATCCAGACGAGGGAAGAGAAGAGGATCAACCTGACTGTTGGCAGTA GAGCCTACTTGCTGCTCAATACATCTGTGATCATCAAATGCCCAGTGCGACGCTTCCAGAAGTCTCTAAT CCAGTGGGAGAAGGATGGCCATTGTCTGAAGAACTCTAAGCAGCTTGGCATCACCAAGTCAGGTTCACTG AAAATCCATAGCCTTGCTGCCCCTGACATTGGTGTATACAGATGCATCGCAGGCTCTGCACAGGAGACGG TCGTTCTCAAGCTCATTGGGACTGACAACCGACTCATCGCACACCCGTCCCTACGAGAGCACGTGAGGGG TCACCCTGGGGTGGACCATAATGAAGCCAATAGTTTGGGAGCCACATGGCATAAAATGCGCCAGATGTGG AATAACAAAAATGATCTTTATCTGCACGGTGGTGAAATGAATAAGCAGCCTTTCTTAAGAGCCCTGTTCG GGCCGTGCCGGACCTCTGAAGGAAATGATAACTCCTGGGAGTTTAAGAACAAGCAGTTTGAAGCAGCCAT GAAACAGGGAGCATACAGCATGGACACAGCCCAATTTGATGAGCTTATAAGAAACATGAGCCAGCTTATG GAGATGGGAGAGGCCAGCGACGATCTCGCATCCCAGATGATATATCAGCTGGTAGCTGAGTTGGCCAAGG CTCAGCCAACGCCTGTGCAGTGGAGGGGCATCCGTGAGGAAGTTGCACCTGAGGCTCAGCTCAGAGGGAA GACAGAAAGAGTTCCTAAAAACCCCACCGTGAAAAACTCAGGCAAGCTGACTTTTAAGCCAAAAGGACCT GTTCTCATGAGGCAAATTCTACCCCCTTCGGTTTCATTTAACAAAACAATAAATGCAAGGATTGGGAATA CAGTGTACATTACAAAAAGCACGGAGATCATCAATATACTTTGCAGCCTCATTACTCCCAGCAAGGAGGC CACATACACATGGACCAAGGATGGAGCGCTGCTGCAACCCTCTGTCAAGATCATTTTGGATGAAACTGGG AAGATGCAAATACGAAATCCTACAAGGAAAGAGCAAGGGATATATGAATGTTCTGTAGCCAGTCATCTGG GTTCCGATGTGGAAAGCTCACTAGTGCTATATGCAGAGGCACCTGTCATCTTGTCTATTGAAAGAAATGT CACCAAACCAGAACACAATCATCTGTCTGTTGTTGTGGGAGGCACTGTGGAGGCAGCCCTTCAAGCAAAT GTGACAATCCACTGTCCTGTAAAAGGTGTCCCTCAGCCTAATGTAACTTGGTTGAAGACAGGAGGATCTC TGAGTGACAATGTTTCCTTGCTTTTCAATGGATCCCTGTTGTTGCAGAGTGCTTCACTCGAAAATGAAGG AACCTACATTTGCACAGCCACCAATGCTCTTGGGAAAGCAGTGGCAACCTCTGGGCTCCGCTTGCTGGAA CGAAGAGAACCAGGCGGTAAGGCTGCGTTCCCTAAGGTCCAGAAAACGCGAGTTCTCCAGGCACCCAGAA GCAGAATAAACAGCAACAACTTAACAGGAAAGTCCCTGCCTCAAGAGCACTTTTGGGAACCTGGCAACTG GACACACTGTTCTGCCACTTGTGGTCCCTTGGGGTTCCGGCTTCAGAGGCCCCGGTGTGTGATGGCCAGT GGTCAGGAAGTGAGTGAAGCCCTGTGTGGTCCCCACAGGAAGCCGCTGGCTGGGTTTCAGTCCTGTAACA TCCGGGATTGCCCAGCAAGGTGGTTTACAAGCATGTGGTCAGAGTGCTCCGCGTCTTGTGGTGAAGGATT TCACAGTCGGCAAGTGACATGCAAGCAGACAAAAGCCAATGGGACTGTGCAGGTGGTGTCCCCAAGGGCA TGTGCTCCTAAAGACAGGCCTCTAGGGAGAAAACCATGTTCCATTCGTCCATGTGTTCAACAGGCCATCG ACCCAGGAAACCAGTGTCCTGGACGTTGCATGGGCCATGCCACGAGGATGCAGCACCACCACACTGCTTG TTCCCACAACAGCTCCAGCTCTGACTGTGAGGACGGAAGGAGACCTGCCTTTGGAAGGAACTGCACCTTG GGGCCATGTGAAGCATGTTGGCGTGTGGGCCCCTGGAAGCCCTGTACAGCAGTCTGTGGCAGGGGCTTCC AGTCTCGCAAAGTCGACTGCATTCACACAGGGAGCTGCAAAATTGTGGCGGACAGATACTGTGTGCAGGT GAAACCAGCTGCCTGGAGGCACTGTCTCGGGCCTTCCTGTGATAGAAACTGCACGGACACAACTCACTAC TGTATGTTCGTAAAGCACCTTAACCTGTGCTCTCTGGCCGTCTACAGACAAAGGTGCTGCCAGTCATGTC AAGAAGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR221800 representing NM_001190374 Red=Cloning site Green=Tags(s) MAVRETPMILDPMASWTGSWWLLTRMIFTVSGLLQATAEKSPGAYFLPEFALSPQGSFLEDTTGEQFLTY RYDDQTSRNAHSEEDKDGNWDAWGDWSDCSRTCGGGASYSLRRCLTGRNCEGQNIRYKTCSNHDCPADAE DFRAQQCSAYNDVQYQGHYYEWIPLYNDPAAPCALKCHARGQSLVVELAPKVLDGTRCNADSLDMCISGI CQIVGCDRQLGSNAKEDNCGVCAGDGSTCRLVRGQTKMHLSPEKKEENVIAVPLGSRSVRITVKGPALIF IESKTLQGSRGEHSFNSPGVFTVENTTIEFQKGADRQTFKIAGPLMADFIFKTRYTVAKGSVVQFFFYQP ISHQWRQTDFFPCTVTCGGGYQLNSAECMDIRLKRVVPDHYCHYYPENVKPKPKLKECSMDPCPSSDGFK EIMPYDHFQPLPRWEHNPWTACSVSCGGGIQRRSFVCVEESMHAEILQVEEWKCMYAPKPTVMQACNLFD CPKWVAMEWSQCTVTCGRGLRYRVVLCINHRGQHVGGCNPQMKLHIKEECVIPIPCYKPREKSPVEAKLP WLKQAQELEETRIATEEPKFIPEPWSACSTTCGPGIQVREVRCRVLLTFTQTEAELPEEECEGPKPPTDR PCLRQACDQSPVSRELGGRLQEKDSEMTYDWEYAGFTPCTATCLGGHQEAIAVCLHTQTHQTVNDSLCDT VHRPPPMSQACNMEPCPPRWHMGSWGACSATCGVGIQTRDVYCLHPEEFPAPPEECREEKPHALRACNQF DCPPSWHIEEWQQCSRTCGGGTQNRRVTCRQLLTDGSFLNLSDELCQGPKASSHKSCARTDCPPQLAAGD WSKCSVTCGVGFQRRKQVCQKLTAKGRRVSVAETLCRDLPGLPLVRPCQMPICSKAKLGTKTKLGERGPQ ILGVRRVYIQTREEKRINLTVGSRAYLLLNTSVIIKCPVRRFQKSLIQWEKDGHCLKNSKQLGITKSGSL KIHSLAAPDIGVYRCIAGSAQETVVLKLIGTDNRLIAHPSLREHVRGHPGVDHNEANSLGATWHKMRQMW NNKNDLYLHGGEMNKQPFLRALFGPCRTSEGNDNSWEFKNKQFEAAMKQGAYSMDTAQFDELIRNMSQLM EMGEASDDLASQMIYQLVAELAKAQPTPVQWRGIREEVAPEAQLRGKTERVPKNPTVKNSGKLTFKPKGP VLMRQILPPSVSFNKTINARIGNTVYITKSTEIINILCSLITPSKEATYTWTKDGALLQPSVKIILDETG KMQIRNPTRKEQGIYECSVASHLGSDVESSLVLYAEAPVILSIERNVTKPEHNHLSVVVGGTVEAALQAN VTIHCPVKGVPQPNVTWLKTGGSLSDNVSLLFNGSLLLQSASLENEGTYICTATNALGKAVATSGLRLLE RREPGGKAAFPKVQKTRVLQAPRSRINSNNLTGKSLPQEHFWEPGNWTHCSATCGPLGFRLQRPRCVMAS GQEVSEALCGPHRKPLAGFQSCNIRDCPARWFTSMWSECSASCGEGFHSRQVTCKQTKANGTVQVVSPRA CAPKDRPLGRKPCSIRPCVQQAIDPGNQCPGRCMGHATRMQHHHTACSHNSSSSDCEDGRRPAFGRNCTL GPCEACWRVGPWKPCTAVCGRGFQSRKVDCIHTGSCKIVADRYCVQVKPAAWRHCLGPSCDRNCTDTTHY CMFVKHLNLCSLAVYRQRCCQSCQEG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001190374
ORF Size	5118 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001190374.1, NP_001177303.1
RefSeq Size	7285 bp
RefSeq ORF	5121 bp
Locus ID	269959
MW	190.2 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.