Dmp1 (NM_016779) Mouse Tagged ORF Clone

CAT#: MR221826

  • TrueORF®

Dmp1 (Myc-DDK-tagged) - Mouse dentin matrix protein 1 (Dmp1)

ORF Plasmid: DDK tGFP



  "NM_016779" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,744.00

CNY 4,560.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AG1; AV020965; Dmp; DMP-1; PP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR221826 representing NM_016779
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGACTGTCATTCTCCTTGTGTTCCTTTGGGGGCTGTCCTGTGCTCTCCCAGTTGCCAGATACCACA
ATACTGAATCTGAAAGCTCTGAAGAGAGGACGGGTGATTTGGCTGGGTCACCACCACCACCCACGAACAG
TGAGTCATCAGAAGAAAGTCAAGCTAGCCCAGAGGGACAGGCAAATAGTGACCACACGGACAGCAGTGAA
TCTGGAGAGGAGCTGGGCTACGACAGAGGCCAGTACAGACCGGCTGGTGGACTCTCTAAGAGTACGGGGA
CCGGCGCCGATAAGGAGGATGATGAAGACGACAGTGGAGATGATACCTTTGGCGATGAGGACAATGATCT
AGGGCCCGAAGAAGGACAGTGGGGAGGACCCTCCAAACTGGACAGTGATGAGGACTCCACAGACACCACA
CAGTCCAGTGAAGACAGCACCTCTCAAGAAAACAGTGCCCAAGATACCCCCAGCGACAGCAAAGACCACG
ACAGTGAGGATGAGGCAGACAGCCGGCCTGAGGCAGGCGACTCCACTCAGGACAGTGAGAGTGAGGAACA
GCGGGTGGGAGGTGGCAGCGAGGGGGAGAGTAGCCACGGGGACGGTTCTGAGTTCGATGATGAAGGGATG
CAGAGCGACGACCCCGAGAGTACCAGGAGCGATCGAGGCCACGCCAGAATGAGCAGCGCTGGTATCAGGT
CGGAAGAATCTAAAGGGGACCACGAGCCCACGAGCACTCAGGATTCAGATGACAGCCAGTCTGTGGAATT
TTCAAGCAGGAAGTCCTTCAGAAGGTCCCACGTCTCTGAGGAAGACTACAGAGGTGAGCTTACTGACAGC
AACAGCAGGGAAACCCAGAGCGACTCCACGGAGGATACGGCCTCCAAGGAGGAAAGCAGGAGCGAGTCTC
AGGAGGACACAGCCGAGAGCCAGTCCCAGGAAGATAGCCCAGAGGGGCAAGACCCCAGCAGTGAGTCCAG
CGAAGAGGCTGGTGAGCCATCCCAGGAAAGCAGCAGCGAATCTCAGGAAGGGGTGACCAGCGAGTCCAGG
GGTGACAACCCAGATAACACAAGTCAGGCAGGAGACCAAGAAGACAGTGAGTCCAGTGAGGAGGACAGCC
TGAACACATTCTCCAGCTCAGAAAGCCAGTCCACCGAGGAGCAAGCTGACAGCGAGTCCAACGAGAGCCT
CAGCCTCTCCGAGGAGAGTCAGGAGTCGGCCCAGGATGGTGACAGCTCCAGCCAGGAAGGCCTGCAGTCC
CAGAGCGCATCCACTGAGAGCAGGAGCCAGGAGAGCCAGTCTGAGCAGGACAGCCGTTCTGAGGAAGACA
GTGACTCTCAGGACAGTAGCCGATCCAAAGAAGAGAGCAACTCCACAGGGAGCGCTTCCAGCAGCGAGGA
GGACATCCGTCCCAAGAACATGGAAGCTGACAGTAGGAAACTAATAGTTGATGCTTACCACAACAAACCC
ATCGGGGACCAAGATGACAATGACTGTCAGGACGGCTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR221826 representing NM_016779
Red=Cloning site Green=Tags(s)

MKTVILLVFLWGLSCALPVARYHNTESESSEERTGDLAGSPPPPTNSESSEESQASPEGQANSDHTDSSE
SGEELGYDRGQYRPAGGLSKSTGTGADKEDDEDDSGDDTFGDEDNDLGPEEGQWGGPSKLDSDEDSTDTT
QSSEDSTSQENSAQDTPSDSKDHDSEDEADSRPEAGDSTQDSESEEQRVGGGSEGESSHGDGSEFDDEGM
QSDDPESTRSDRGHARMSSAGIRSEESKGDHEPTSTQDSDDSQSVEFSSRKSFRRSHVSEEDYRGELTDS
NSRETQSDSTEDTASKEESRSESQEDTAESQSQEDSPEGQDPSSESSEEAGEPSQESSSESQEGVTSESR
GDNPDNTSQAGDQEDSESSEEDSLNTFSSSESQSTEEQADSESNESLSLSEESQESAQDGDSSSQEGLQS
QSASTESRSQESQSEQDSRSEEDSDSQDSSRSKEESNSTGSASSSEEDIRPKNMEADSRKLIVDAYHNKP
IGDQDDNDCQDGY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016779
ORF Size 1509 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016779.2, NP_058059.2
RefSeq Size 2760 bp
RefSeq ORF 1512 bp
Locus ID 13406
UniProt ID O55188
MW 54.4 kDa
Gene Summary May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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