Sema6b (NM_013662) Mouse Tagged ORF Clone

CAT＃: MR221958

  • TrueORF®

Sema6b (Myc-DDK-tagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B (Sema6b), transcript variant 1

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_013662" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,496.00

CNY 7,130.00

货期*
现货
规格
    • 10 ug

Product images

经常一起买 (4)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Sema; Seman; semaZ; VIb
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR221958 representing NM_013662
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGACCCCGCGAGTGCCCCCTCCACGCCCGGCCCTGTCGTTTTTCCTGCTGTTGCTCCTGGGGGTCA
CCTATGGCCTTTTCCCAGAAGAACCACCTCCACTCAGCGTGGCTCCCAGGGACTACTTGAGCCACTATCC
CGTGTTCGTGGGCAGCGGGCCTGGTCGTCTGACCGCTGCGGAGGGTGCTGAGGACCTCAACATCCAGAGA
GTGCTACGTGTTAACAGGACACTGTTCATTGGGGACAGAGATAACCTGTACCAAGTAGAACTGGAGCCAT
CCACTTCCACGGAGCTGCGATATCAGAGGAAGCTGACCTGGCGCTCCAACCCCAGTGACATCGATGTGTG
TCGAATGAAGGGCAAGCAAGAGGGTGAGTGTCGGAACTTTGTCAAGGTGCTCCTGCTTCGCGATGAGTCC
ACGCTGTTCGTGTGCGGCTCCAATGCGTTCAATCCCATCTGCGCCAATTACAGTATGGACACACTGCAGC
TTCTTGGTGACAGCATCAGCGGTATGGCCCGCTGCCCCTATGACCCCAAACATGCCAATGTCGCCCTCTT
CTCAGATGGGATGCTCTTCACAGCCACGGTGACTGACTTCCTAGCCATCGACGCTGTTATCTACCGCAGC
CTTGGGGACCGGCCCACACTGCGCACAGTAAAGCACGACTCCAAGTGGTTTAAAGAGCCGTACTTTGTGC
ATGCAGTGGAGTGGGGAAGCCACGTCTATTTCTTCTTCCGGGAGATCGCCATGGAGTTTAACTACCTGGA
AAAGGTGGTGGTGTCTCGAGTGGCCCGTGTGTGCAAGAATGACGTGGGCGGCTCGCCACGAGTGCTGGAG
AAGCAGTGGACTTCCTTCCTGAAGGCCCGGCTCAACTGCTCAGTGCCAGGGGATTCACACTTCTACTTCA
ACGTCCTGCAGGCTGTGACGGGCGTGGTGAGCCTGGGTGGTCGTCCGGTGATCCTCGCTGTCTTCTCAAC
TCCTAGCAACAGCATCCCCGGCTCAGCCGTCTGCGCCTTTGACATGAACCAAGTGGCTGCTGTGTTTGAA
GGCCGCTTCCGGGAACAGAAGTCGCCTGAGTCGATCTGGACACCAGTGCCTGAAGACCAAGTGCCACGGC
CCAGGCCTGGGTGCTGCGCGGCACCTGGCATGCAGTACAACGCGTCCAGTGCCCTCCCTGACGAGATCCT
CAACTTTGTAAAGACCCACCCACTGATGGATGAGGCAGTACCTTCCCTGGGTCACTCGCCGTGGATTGTG
AGAACTCTGATGCGGCACCAGCTGACCCGAGTGGCTGTGGATGTGGGTGCGGGTCCATGGGGCAATCAGA
CAATAGTCTTCCTTGGCTCCGAGGCTGGCACAGTCCTCAAATTTCTTGTGAAGCCCAACGCCAGTGTCTC
AGGGACCACAGGGCCCAGCATCTTTTTGGAGGAGTTTGAGACCTACCGGCCAGACAGGTGTGGACGACCC
AGCAGTGCTGGTGAGTGGGGGCAGCGACTTCTCAGCCTGGAGCTGGATGCAGCCTCAGGGGGCCTGCTGG
CAGCCTTCCCCCGCTGTGTGGTTCGTGTTCCTGTTGCCCGTTGCCAGCTGTACTCGGGGTGCATGAAGAA
CTGTATTGGCAGCCAGGATCCATACTGCGGGTGGGCCCCTGATGGCTCCTGCATCTTCCTCAGACCAGGA
ACTAGTGCCACGTTTGAGCAAGATGTGTCCGGGGCCAGCACCTCTGGCTTAGGTGACTGCACTGGACTCT
TGCGCGCCAGCCTCTCAGATGACCGCGCAGGGCTGGTGTCGGTGAACCTGCTAGTGACGTCCTCGGTGGC
AGCGTTCGTGGTGGGTGCCGTGGTGTCCGGCTTCAGTGTAGGCTGGTTCGTGGGTCTCCGCGAGCGGCGG
GAGCTGGCCCGGCGCAAGGACAAGGAGGCCATTCTGGCGCACGGCGGCAGCGAGGCAGTTCTGAGCGTGA
GCCGACTAGGCGAGCGCAGGGGGACCGGGCCCGGGGGTCGTGGAGGAGCCGGCGGCGGTCCCGGAGGTCC
CCCGGAGGCCCTGCTGGCCCCGCTTATGCAGAATGGCTGGACAAAGGCAGCGCTGCTGCATGGTGGCCCT
CACGACCTGGACACGGGGCTGCTGCCCACCCCAGAGCAGACGCCTCTACCCCAGAAACGCCTGCCCACGC
CACACCCACACGCCCACGCCCTGGGGTCTCGAGCCTGGGACCACAGCCACGCGCTGCTGTCGGCCTCAGC
CTCCACCTCCTTGCTCCTACTGGCGCCCGCCCGCGCCTCTGAGCAGCCCCAGGTGCCAGCCGAGCCGGGC
CCGGAATCTCGCCTCTGCGCCCCCAGATCCTGCCGGGCCTCTCACCCAGGCGACTTCCCGCTCACGCCCC
ACGCCAGCCCGG[AT]CCGCCGGCGGGTGGTGTCCGCACCCACGGGCCCCTTGGACCCTTCGGTGGGCGA
CGGCCTGCCCGGGCCATGGAGCCCACCTGCAACCAGCAGCCTGCGGAGGCCGGGTCCCCACGGGCCCCCC
ACAGCCGCCCTACGGCGCACACACACATTCAACAGCGGCGAGGCTCGGCCCGGGGGTCATCGTCCTCGCC
GCCACCCACCCGCGGACTCTACACACTTGCTGCCCTGCGGGACGGGCGAGCGGACTGCACCCCCGGTACC
C

ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
TTACAAGGATGACGACGATAAGGTTTAA
>MR221958 representing NM_013662
Red=Cloning site Green=Tags(s)

MWTPRVPPPRPALSFFLLLLLGVTYGLFPEEPPPLSVAPRDYLSHYPVFVGSGPGRLTAAEGAEDLNIQR
VLRVNRTLFIGDRDNLYQVELEPSTSTELRYQRKLTWRSNPSDIDVCRMKGKQEGECRNFVKVLLLRDES
TLFVCGSNAFNPICANYSMDTLQLLGDSISGMARCPYDPKHANVALFSDGMLFTATVTDFLAIDAVIYRS
LGDRPTLRTVKHDSKWFKEPYFVHAVEWGSHVYFFFREIAMEFNYLEKVVVSRVARVCKNDVGGSPRVLE
KQWTSFLKARLNCSVPGDSHFYFNVLQAVTGVVSLGGRPVILAVFSTPSNSIPGSAVCAFDMNQVAAVFE
GRFREQKSPESIWTPVPEDQVPRPRPGCCAAPGMQYNASSALPDEILNFVKTHPLMDEAVPSLGHSPWIV
RTLMRHQLTRVAVDVGAGPWGNQTIVFLGSEAGTVLKFLVKPNASVSGTTGPSIFLEEFETYRPDRCGRP
SSAGEWGQRLLSLELDAASGGLLAAFPRCVVRVPVARCQLYSGCMKNCIGSQDPYCGWAPDGSCIFLRPG
TSATFEQDVSGASTSGLGDCTGLLRASLSDDRAGLVSVNLLVTSSVAAFVVGAVVSGFSVGWFVGLRERR
ELARRKDKEAILAHGGSEAVLSVSRLGERRGTGPGGRGGAGGGPGGPPEALLAPLMQNGWTKAALLHGGP
HDLDTGLLPTPEQTPLPQKRLPTPHPHAHALGSRAWDHSHALLSASASTSLLLLAPARASEQPQVPAEPG
PESRLCAPRSCRASHPGDFPLTPHASPXXRRRVVSAPTGPLDPSVGDGLPGPWSPPATSSLRRPGPHGPP
TAALRRTHTFNSGEARPGGHRPRRHPPADSTHLLPCGTGERTAPPVP

TRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-NotI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_013662
ORF Size 2661 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_013662.1, NM_013662.2, NP_038690.1
RefSeq Size 3847 bp
RefSeq ORF 2661 bp
Locus ID 20359
UniProt ID O54951
MW 95.6 kDa
Gene Summary Functions as a cell surface repellent for mossy fibers of developping neurons in the hippocampus where it plays a role in axon guidance (PubMed:20484647). May function through the PLXNA4 receptor expressed by mossy cell axons (PubMed:20484647).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
MC222376 Sema6b (untagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B (Sema6b), transcript variant 1, (10ug)
    • 10 ug

CNY 6,496.00

MG221958 Sema6b (tGFP-tagged) - Mouse sema domain transmembrane domain (TM) and cytoplasmic domain (semaphorin) 6B (Sema6b) transcript variant 1, (10ug)
    • 10 ug

CNY 7,890.00

MR221958L3 Lenti ORF clone of Sema6b (Myc-DDK-tagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B (Sema6b), transcript variant 1
    • 10 ug

CNY 9,030.00

MR221958L4 Lenti ORF clone of Sema6b (mGFP-tagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B (Sema6b), transcript variant 1
    • 10 ug

CNY 9,030.00

You may also need
plasmid icon Empty Vector
protein iconDDK/Flag Antibody

其它Sema6b产品

Expression Plasmids
Lentiviral Particles
CRISPR
RNAi
Proteins
Antibodies
相关产品 (9)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

    • 10 ug

CNY 5,420.00

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

    • 1 ml in 1 vial

CNY 4,090.00

TA50011-100

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

【特别福利】购一抗正装产品，免费领10ul抗体试用装

    • 100 ul

CNY 1,050.00

MC222376

Sema6b (untagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B (Sema6b), transcript variant 1, (10ug)

    • 10 ug

CNY 6,496.00

MR221958L3V

Lenti ORF particles, Sema6b (Myc-DDK-tagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B (Sema6b), transcript variant 1, 200ul, >10^7 TU/mL

    • 200 ul

CNY 13,870.00 暂停销售

MR221958L4V

Lenti ORF particles, Sema6b (GFP-tagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B (Sema6b), transcript variant 1, 200ul, >10^7 TU/mL

    • 200 ul

CNY 15,390.00 暂停销售

MG221958

Sema6b (tGFP-tagged) - Mouse sema domain transmembrane domain (TM) and cytoplasmic domain (semaphorin) 6B (Sema6b) transcript variant 1, (10ug)

    • 10 ug

CNY 7,890.00

MR221958L3

Lenti ORF clone of Sema6b (Myc-DDK-tagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B (Sema6b), transcript variant 1

    • 10 ug

CNY 9,030.00

MR221958L4

Lenti ORF clone of Sema6b (mGFP-tagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B (Sema6b), transcript variant 1

    • 10 ug

CNY 9,030.00

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