Psmd13 (NM_011875) Mouse Tagged ORF Clone

CAT#: MR222043

  • TrueORF®

Psmd13 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 13 (Psmd13)

ORF Plasmid: DDK tGFP



  "NM_011875" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,330.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms S11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR222043 representing NM_011875
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGATGTGCCGGCCTTCTTACAGCAGAGTCAGAGCTCCGGGCCGGGCCAGGCTGCCGTTTGGCACC
GTTTGGAAGAGCTGTACACGAAGAAGTTGTGGCATCAGCTGACACTTGAGGTGCTTGATTTTGTGCAGGA
CCCATGCTTTGCCCAAGGAGATGGTCTCATTAAGCTTTATGAAAACTTCATCAGTGAGTTTGAGCACAGG
GTAAACCCTCTGTCCCTGGTAGAAATAATTCTCCATGTGGTTAGACAGATGACCGATCCTAATGTGGCTC
TTACTTTTCTGGAAAAGACTCGTGAGAAGGTAAAAAGTAGTGATGAAGCCGTGATCTTGTGTAAGACGGC
AATCGGAGCTCTAAAATTAAACATCGGCGATCTGCAGGCTACAAAGGAAACCATTGAAGATGTTGAAGAG
ATGCTCAACAATCTCCCTGGTGTGACGTCCGTTCACAGTCGTTTCTATGATCTCTCCAGTAAATACTATC
AAACAATCGGAAACCACGCGTCCTACTACAAAGATGCTCTACGGTTTCTGGGCTGTGTGGATATCAAGGA
CCTGCCAGTGTCAGAACAGCAAGAGAGAGCCTTCACACTGGGGCTTGCTGGACTTCTCGGAGAGGGAGTT
TTTAACTTTGGAGAACTTCTCATGCACCCTGTGCTGGAGTCACTGAGGGACACTGACCGGCAGTGGCTGA
TTGACACCCTGTACGCTTTCAATAGCGGTGCTGTGGATAGGTTCCAGACACTGAAGTGTGCCTGGGGCCA
GCAGCCTGACTTAGCAGCCAACGAAGCCCAGCTTCTGAGGAAGATCCAGCTGTTGTGCCTTATGGAGATG
ACTTTCACACGGCCTGCCAACCACAGACAACTCACTTTTGAAGAAATTGCCAAAAGTGCCAAAATCACTG
TCAACAAGGTGGAGTTGCTGGTGATGAAGGCACTCTCGGTGGGGCTGGTGAGAGGCAGCATAGACGAGGT
GGACAAGCGGGTTCACATGACGTGGGTGCAGCCGCGAGTGCTGGATTTGCAGCAGATCAAGGGGATGAAG
GACCGTCTGGAGCTTTGGTGCACTGATGTGAAGAGCATGGAGATGCTGGTGGAACACCAGGCTCAGGACA
TCCTCACC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>MR222043 representing NM_011875
Red=Cloning site Green=Tags(s)

MKDVPAFLQQSQSSGPGQAAVWHRLEELYTKKLWHQLTLEVLDFVQDPCFAQGDGLIKLYENFISEFEHR
VNPLSLVEIILHVVRQMTDPNVALTFLEKTREKVKSSDEAVILCKTAIGALKLNIGDLQATKETIEDVEE
MLNNLPGVTSVHSRFYDLSSKYYQTIGNHASYYKDALRFLGCVDIKDLPVSEQQERAFTLGLAGLLGEGV
FNFGELLMHPVLESLRDTDRQWLIDTLYAFNSGAVDRFQTLKCAWGQQPDLAANEAQLLRKIQLLCLMEM
TFTRPANHRQLTFEEIAKSAKITVNKVELLVMKALSVGLVRGSIDEVDKRVHMTWVQPRVLDLQQIKGMK
DRLELWCTDVKSMEMLVEHQAQDILT

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_011875
ORF Size 1128 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_011875.4, NP_036005.1
RefSeq Size 1563 bp
RefSeq ORF 1131 bp
Locus ID 23997
UniProt ID Q9WVJ2
MW 42.8 kDa
Gene Summary Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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