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Rbbp4 (NM_009030) Mouse Tagged ORF Clone
CAT＃: MR222109
- TrueORF®
Rbbp4 (Myc-DDK-tagged) - Mouse retinoblastoma binding protein 4 (Rbbp4)
ORF Plasmid: tGFP
"NM_009030" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | mRbAp48; RBAP48 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222109 representing NM_009030
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACAAGGAAGCGGCCTTTGACGACGCAGTGGAAGAACGGGTGATCAACGAGGAGTACAAAATAT GGAAGAAGAACACCCCTTTTCTTTATGATTTGGTGATGACCCATGCCCTGGAGTGGCCCAGCTTAACTGC CCAGTGGCTTCCAGATGTGACCAGGCCTGAAGGGAAAGATTTCAGCATTCATCGGCTTGTCCTGGGAACA CACACATCTGATGAACAGAACCACCTGGTGATTGCCAGTGTCCAGCTCCCTAATGATGATGCTCAGTTTG ATGCATCCCATTATGACAGTGAAAAAGGAGAATTTGGAGGTTTTGGTTCTGTCAGTGGGAAAATTGAAAT AGAAATCAAGATCAACCATGAAGGAGAAGTGAACAGGGCCCGGTACATGCCTCAGAACCCCTGCATCATT GCAACAAAGACTCCATCCAGTGATGTTCTTGTTTTTGACTACACAAAGCACCCTTCTAAACCAGACCCTT CTGGAGAATGCAACCCCGATTTGCGTCTCCGTGGACATCAGAAGGAAGGTTATGGGCTTTCTTGGAATCC AAATCTCAGTGGGCACTTACTTAGTGCTTCAGATGACCATACCATCTGCCTGTGGGACATCAGTGCAGTT CCAAAAGAAGGAAAGGTGGTGGATGCAAAGACCATCTTCACGGGGCATACAGCAGTAGTGGAGGACGTGT CCTGGCATCTGCTCCACGAGTCTCTATTTGGCTCAGTTGCTGATGACCAGAAGCTTATGATTTGGGACAC TCGTTCAAACAATACTTCCAAGCCAAGCCACTCGGTTGATGCTCACACAGCTGAAGTGAATTGCTTATCT TTCAATCCTTATAGTGAGTTCATTCTTGCCACAGGATCGGCTGACAAGACTGTTGCCTTGTGGGATCTGA GGAATCTGAAACTCAAGTTGCACTCCTTTGAATCCCATAAGGATGAAATATTCCAAGTTCAGTGGTCACC TCACAATGAGACTATCTTAGCTTCCAGTGGTACCGATCGTAGGCTGAATGTCTGGGATCTAAGTAAAATT GGAGAAGAGCAGTCCCCAGAAGATGCAGAGGATGGCCCACCAGAATTGTTGTTTATTCATGGTGGTCACA CTGCCAAGATATCTGACTTCTCCTGGAATCCCAATGAGCCTTGGGTGATTTGTTCTGTATCAGAAGACAA TATCATGCAAGTGTGGCAGATGGCGGAGAACATTTACAATGATGAAGATCCTGAAGGAAGTGTGGATCCA GAGGGACAAGGGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222109 representing NM_009030
Red=Cloning site Green=Tags(s) MADKEAAFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPEGKDFSIHRLVLGT HTSDEQNHLVIASVQLPNDDAQFDASHYDSEKGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNPCII ATKTPSSDVLVFDYTKHPSKPDPSGECNPDLRLRGHQKEGYGLSWNPNLSGHLLSASDDHTICLWDISAV PKEGKVVDAKTIFTGHTAVVEDVSWHLLHESLFGSVADDQKLMIWDTRSNNTSKPSHSVDAHTAEVNCLS FNPYSEFILATGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLNVWDLSKI GEEQSPEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQVWQMAENIYNDEDPEGSVDP EGQGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009030 |
| ORF Size | 1275 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009030.3, NP_033056.2 |
| RefSeq Size | 4407 bp |
| RefSeq ORF | 1278 bp |
| Locus ID | 19646 |
| UniProt ID | Q60972 |
| MW | 48.1 kDa |
| Gene Summary | Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209297 | Rbbp4 (untagged) - Mouse retinoblastoma binding protein 4 (Rbbp4), (10ug) |
CNY 3,990.00 |
|
| MG222109 | Rbbp4 (tGFP-tagged) - Mouse retinoblastoma binding protein 4 (Rbbp4), (10ug) |
CNY 7,088.00 |
|
| MR222109L3 | Lenti ORF clone of Rbbp4 (Myc-DDK-tagged) - Mouse retinoblastoma binding protein 4 (Rbbp4) |
CNY 6,180.00 |
|
| MR222109L4 | Lenti ORF clone of Rbbp4 (mGFP-tagged) - Mouse retinoblastoma binding protein 4 (Rbbp4) |
CNY 6,180.00 |
