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Hsph1 (NM_013559) Mouse Tagged ORF Clone
CAT＃: MR222136
- TrueORF®
Hsph1 (Myc-DDK-tagged) - Mouse heat shock 105kDa/110kDa protein 1 (Hsph1)
ORF Plasmid: tGFP
"NM_013559" in other vectors (4)
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CNY 8,550.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 105kDa; AI790491; hsp-E7I; Hsp105; Hsp110; hsp110/105 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222136 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGTGGTTGGGCTAGACGTAGGCTCACAGAGCTGCTACATTGCGGTGGCGCGGGCCGGGGGCATCG AGACCATCGCCAACGAGTTCAGCGACCGCTGCACCCCGTCAGTCATATCATTTGGATCAAAAAACAGAAC AATTGGAGTTGCAGCCAAAAACCAGCAAATCACTCATGCAAACAATACGGTCTCTAGCTTTAAGAGATTT CATGGCAGAGCATTCAATGACCCCTTCATTCAGAAGGAAAAGGAGAACCTGAGCTATGATTTGGTCCCAA TGAAAAATGGTGGCGTGGGAATAAAGGTCATGTACATGGATGAAGAACATTTCTTCAGTGTGGAGCAGAT AACAGCCATGCTGCTGACTAAGTTAAAGGAAACTGCAGAAAACAACCTCAAGAAGCCAGTGACAGACTGT GTCATCTCAGTCCCATCCTTCTTCACAGATGCTGAGCGAAGGTCTGTGCTGGATGCTGCGCAGATTGTGG GCTTGAACTGCTTGCGGCTCATGAATGACATGACGGCTGTTGCTTTGAATTATGGGATTTATAAGCAAGA TCTCCCGAATGCCGAGGAGAAGCCACGGGTGGTGGTGTTTGTTGACATGGGACACTCATCTTTCCAAGTG TCTGCCTGTGCTTTTAACAAAGGAAAACTGAAGGTTCTAGGCACAGCTTTTGATCCCTTCTTAGGAGGAA AGAACTTTGATGAGAAGCTAGTAGAACATTTTTGTGCTGAATTTAAAACCAAGTACAAATTGGATGCAAA ATCCAAAATTCGAGCCCTCCTTCGTCTCCATCAGGAGTGTGAAAAGTTGAAAAAGCTCATGAGTTCTAAC AGCACGGACCTGCCGCTGAACATCGAGTGCTTTATGAATGACAAGGATGTCTCTGGGAAGATGAACAGGT CACAGTTTGAAGAACTGTGTGCTGAGCTCCTGCAAAAAATAGAGGTCCCCCTTCACTCGTTGATGGCACA GACTCAGCTCAAGGCTGAAGATGTGAGTGCCATTGAGATAGTGGGAGGTGCCACAAGAATCCCAGCTGTG AAAGAAAGAATTGCCAAGTTCTTTGGAAAAGATGTCAGCACCACGCTCAATGCAGACGAAGCTGTGGCCA GAGGCTGTGCACTGCAGTGTGCAATTCTTTCTCCGGCATTTAAAGTTAGAGAGTTCTCTGTCACCGATGC AGTTCCTTTTCCAATATCTCTGGTCTGGAACCACGACTCGGAAGAAACGGAAGGTGTGCACGAGGTGTTC AGTCGGAACCATGCTGCTCCTTTCTCCAAAGTGCTCACCTTCCTGAGAAGGGGGCCCTTTGAGCTAGAAG CTTTCTATTCTGACCCTCAAGGAGTTCCATATCCAGAAGCAAAAATAGGCCGTTTTGTTGTTCAGAATGT TTCTGCACAGAAAGATGGAGAGAAGTCGAGAGTGAAGGTCAAAGTGCGTGTGAACACACATGGCATCTTC ACCATCTCCACGGCTTCCATGGTGGAGAAGGTCCCGACCGAGGAAGAGGATGGCTCCTCTCTCGAGGCAG ACATGGAATGTCCAAACCAGAGGCCAACAGAAAGCTCGGATGTGGATAAAAATATCCAGCAAGACAACAG TGAAGCTGGAACACAGCCCCAGGTACAAACTGATGGTCAACAAACCTCACAGTCTCCCCCTTCACCTGAA CTTACCTCAGAAGAAAGCAAAACCCCAGATGCTGACAAAGCAAATGAAAAGAAAGTTGATCAGCCTCCAG AAGCCAAGAAACCTAAAATAAAGGTGGTAAATGTTGAGCTGCCTGTAGAAGCCAACTTGGTATGGCAGTT AGGGAGAGACCTTCTTAACATGTATATTGAGACAGAGGGCAAGATGATCATGCAAGACAAGCTGGAGAAG GAGCGGAACGACGCCAAGAACGCCGTGGAGGAGTGTGTATATGAGTTCAGGGACAAGCTATGTGGACCAT ATGAGAAATTCATATGTGAGCAGGAACATGAGAAGTTCTTGAGGCTTCTAACAGAGACGGAAGACTGGCT GTATGAGGAAGGGGAGGACCAGGCTAAGCAGGCATACATTGACAAGTTGGAAGAGCTGATGAAAATGGGC ACTCCTGTTAAAGTCAGATTTCAAGAAGCTGAGGAACGACCGAAAGTGTTGGAGGAGCTGGGGCAGCGCC TGCAGCACTATGCCAAGATTGCAGCGGACTTCAGAGGCAAGGATGAGAAATACAACCACATTGATGAATC AGAAATGAAGAAGGTTGAGAAGTCTGTTAATGAGGTGATGGAGTGGATGAATAATGTCATGAATGCTCAG GCTAAAAGAAGTCTTGATCAAGACCCTGTTGTTCGAACTCATGAAATCAGAGCGAAGGTCAAGGAATTGA ACAATGTTTGTGAACCTGTTGTAACTCAACCCAAACCAAAAATCGAGTCACCTAAACTGGAGAGAACTCC AAATGGCCCAAATATTGACAAGAAAGAAGATTTAGAAGGCAAAAATAATCTTGGTGCTGAAGCTCCGCAT CAGAATGGTGAATGCCACCCTAATGAGAAGGGCTCTGTCAACATGGACCTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222136 protein sequence
Red=Cloning site Green=Tags(s) MSVVGLDVGSQSCYIAVARAGGIETIANEFSDRCTPSVISFGSKNRTIGVAAKNQQITHANNTVSSFKRF HGRAFNDPFIQKEKENLSYDLVPMKNGGVGIKVMYMDEEHFFSVEQITAMLLTKLKETAENNLKKPVTDC VISVPSFFTDAERRSVLDAAQIVGLNCLRLMNDMTAVALNYGIYKQDLPNAEEKPRVVVFVDMGHSSFQV SACAFNKGKLKVLGTAFDPFLGGKNFDEKLVEHFCAEFKTKYKLDAKSKIRALLRLHQECEKLKKLMSSN STDLPLNIECFMNDKDVSGKMNRSQFEELCAELLQKIEVPLHSLMAQTQLKAEDVSAIEIVGGATRIPAV KERIAKFFGKDVSTTLNADEAVARGCALQCAILSPAFKVREFSVTDAVPFPISLVWNHDSEETEGVHEVF SRNHAAPFSKVLTFLRRGPFELEAFYSDPQGVPYPEAKIGRFVVQNVSAQKDGEKSRVKVKVRVNTHGIF TISTASMVEKVPTEEEDGSSLEADMECPNQRPTESSDVDKNIQQDNSEAGTQPQVQTDGQQTSQSPPSPE LTSEESKTPDADKANEKKVDQPPEAKKPKIKVVNVELPVEANLVWQLGRDLLNMYIETEGKMIMQDKLEK ERNDAKNAVEECVYEFRDKLCGPYEKFICEQEHEKFLRLLTETEDWLYEEGEDQAKQAYIDKLEELMKMG TPVKVRFQEAEERPKVLEELGQRLQHYAKIAADFRGKDEKYNHIDESEMKKVEKSVNEVMEWMNNVMNAQ AKRSLDQDPVVRTHEIRAKVKELNNVCEPVVTQPKPKIESPKLERTPNGPNIDKKEDLEGKNNLGAEAPH QNGECHPNEKGSVNMDLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013559 |
| ORF Size | 2577 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013559.2, NP_038587.2 |
| RefSeq Size | 3478 bp |
| RefSeq ORF | 2577 bp |
| Locus ID | 15505 |
| UniProt ID | Q61699 |
| MW | 96.4 kDa |
| Gene Summary | Acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering client/substrate protein release (By similarity). Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities (PubMed:14644449, PubMed:15292236).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222144 | Hsph1 (untagged) - Mouse heat shock 105kDa/110kDa protein 1 (Hsph1), (10ug) |
CNY 10,930.00 |
|
| MG222136 | Hsph1 (tGFP-tagged) - Mouse heat shock 105kDa/110kDa protein 1 (Hsph1), (10ug) |
CNY 11,032.00 |
|
| MR222136L3 | Lenti ORF clone of Hsph1 (Myc-DDK-tagged) - Mouse heat shock 105kDa/110kDa protein 1 (Hsph1) |
CNY 10,450.00 |
|
| MR222136L4 | Lenti ORF clone of Hsph1 (mGFP-tagged) - Mouse heat shock 105kDa/110kDa protein 1 (Hsph1) |
CNY 10,450.00 |
