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Nlgn2 (NM_198862) Mouse Tagged ORF Clone
CAT＃: MR222168
- TrueORF®
Nlgn2 (Myc-DDK-tagged) - Mouse neuroligin 2 (Nlgn2)
ORF Plasmid: tGFP
"NM_198862" in other vectors (4)
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CNY 6,144.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NL2; NLG2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222168 representing NM_198862
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGGCTCCTGGCGTTGTGTCTGGTGGGGCTGGCTGGGGCTCAACGGGGAGGAGGGGGTCCCGGCGGCG GCGCCCCGGGCGGCCCAGGCCTGGGCCTCGGCAGCCTCGGGGAGGAGCGCTTCCCCGTGGTGAACACAGC CTACGGGCGAGTGCGCGGTGTGCGGCGCGAGCTCAACAACGAGATCCTGGGCCCGGTCGTGCAGTTCTTG GGCGTGCCCTACGCCACGCCGCCCTTGGGCGCCCGCCGCTTCCAGCCGCCTGAGGCACCTGCCTCGTGGC CCGGCGTGCGCAACGCCACCACCCTGCCGCCCGCCTGCCCGCAGAACCTGCACGGGGCCCTGCCGGCCAT CATGCTGCCTGTGTGGTTCACCGACAACTTGGAGGCGGCCGCCACCTACGTGCAGAACCAGAGCGAGGAC TGCCTGTACCTCAACCTCTACGTGCCCACTGAGGACGGTCCGCTCACAAAAAAACGTGACGAGGCGACGC TCAATCCGCCAGACACAGATATCCGGGACTCTGGGAAGAAACCGGTCATGCTGTTTCTACACGGCGGCTC CTACATGGAAGGCACCGGGAACATGTTTGACGGCTCAGTCCTGGCTGCCTATGGCAATGTCATCGTAGTC ACACTCAACTACCGTCTTGGGGTGCTCGGTTTTCTCAGCACTGGTGACCAGGCTGCAAAAGGCAACTATG GGCTCCTGGACCAGATCCAGGCCCTGCGCTGGCTCAGTGAAAACATTGCCCACTTTGGAGGTGACCCTGA ACGCATCACTATCTTTGGGTCTGGTGCAGGGGCCTCCTGTGTCAACTTGCTGATCCTTTCCCACCACTCA GAAGGACTGTTCCAGAAGGCCATTGCTCAAAGTGGTACTGCCATTTCCAGCTGGTCTGTCAACTACCAGC CGCTCAAGTACACGCGGCTGCTGGCGGCCAAAGTGGGCTGTGACCGAGAAGACAGCACTGAAGCTGTGGA GTGTCTGCGCCGGAAGTCTTCCCGGGAGCTAGTGGACCAGGATGTACAGCCTGCCCGCTACCACATTGCC TTTGGGCCTGTGGTGGACGGCGACGTAGTCCCTGATGACCCCGAGATCCTCATGCAACAGGGGGAATTCC TCAACTACGACATGCTCATTGGTGTCAACCAGGGAGAGGGTCTCAAGTTCGTGGAGGACTCTGCAGAGAG TGAGGACGGTGTGTCTGCCAGCGCCTTTGACTTCACCGTCTCCAACTTTGTGGACAACTTGTACGGGTAC CCAGAAGGCAAGGACGTGCTTCGAGAGACCATCAAGTTCATGTACACGGACTGGGCTGACAGGGACAATG GCGAGATGCGGCGGAAGACCCTGCTGGCGCTCTTTACCGACCACCAGTGGGTCGCCCCGGCTGTGGCCAC CGCCAAGCTGCATGCCGACTACCAGTCCCCCGTCTACTTTTACACTTTCTACCACCACTGCCAGGCAGAG GGCCGGCCAGAGTGGGCAGACGCAGCGCACGGGGACGAGCTGCCCTACGTCTTTGGTGTCCCCATGGTGG GCGCCACTGACCTCTTCCCCTGCAACTTCTCCAAGAACGATGTCATGCTCAGCGCAGTAGTCATGACCTA TTGGACCAACTTCGCCAAGACTGGTGACCCCAACCAGCCTGTGCCACAGGACACCAAGTTCATCCACACC AAGCCCAACCGCTTTGAAGAGGTAGTGTGGAGCAAGTTCAACAGCAAGGAAAAGCAGTATCTGCACATAG GCTTGAAACCACGCGTGCGCGACAACTACCGTGCCAACAAGGTGGCCTTCTGGCTGGAGCTCGTGCCCCA CCTGCACAACCTGCACACAGAGCTCTTCACCACCACCACTCGCCTGCCTCCCTATGCCACACGCTGGCCA CCTCGCACACCTGGTCCTGGCACTTCCGGCACACGCCGTCCTCCCCCACCTGCCACTCTGCCACCTGAGT CTGATATTGACCTAGGCCCAAGGGCCTATGACCGCTTCCCCGGTGACTCGAGGGACTACTCCACGGAGCT AAGCGTGACTGTGGCAGTGGGTGCCTCCCTCCTCTTCCTCAACATCCTTGCCTTTGCCGCCCTCTATTAC AAGCGGGACCGGCGCCAGGAGCTGCGGTGCCGGAGGCTTAGCCCACCAGGAGGCTCAGGCTCAGGTGTGC CTGGTGGGGGCCCCCTGCTTCCCACTGCTGGCCGTGAGCTACCCCCGGAGGAGGAGCTAGTATCGCTGCA GCTGAAGCGGGGTGGTGGTGTTGGGGCGGACCCTGCTGAGGCCCTGCGCCCTGCCTGTCCACCCGACTAT ACCCTGGCCTTGCGCCGGGCACCGGACGATGTGCCTCTCTTGGCCCCCGGGGCCCTAACCCTGCTGCCTA GTGGCCTGGGGCCCCCGCCCCCGCCCCCACCCCCTTCTCTCCATCCCTTTGGGCCCTTCCCACCCCCACC CCCTACTGCTACCAGCCACAACAACACGCTACCCCATCCCCACTCCACCACTCGGGTACAAGCTT CAAGCTTAACTAGCTAGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATC TGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAAAC >MR222168 representing NM_198862
Red=Cloning site Green=Tags(s) MWLLALCLVGLAGAQRGGGGPGGGAPGGPGLGLGSLGEERFPVVNTAYGRVRGVRRELNNEILGPVVQFL GVPYATPPLGARRFQPPEAPASWPGVRNATTLPPACPQNLHGALPAIMLPVWFTDNLEAAATYVQNQSED CLYLNLYVPTEDGPLTKKRDEATLNPPDTDIRDSGKKPVMLFLHGGSYMEGTGNMFDGSVLAAYGNVIVV TLNYRLGVLGFLSTGDQAAKGNYGLLDQIQALRWLSENIAHFGGDPERITIFGSGAGASCVNLLILSHHS EGLFQKAIAQSGTAISSWSVNYQPLKYTRLLAAKVGCDREDSTEAVECLRRKSSRELVDQDVQPARYHIA FGPVVDGDVVPDDPEILMQQGEFLNYDMLIGVNQGEGLKFVEDSAESEDGVSASAFDFTVSNFVDNLYGY PEGKDVLRETIKFMYTDWADRDNGEMRRKTLLALFTDHQWVAPAVATAKLHADYQSPVYFYTFYHHCQAE GRPEWADAAHGDELPYVFGVPMVGATDLFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHT KPNRFEEVVWSKFNSKEKQYLHIGLKPRVRDNYRANKVAFWLELVPHLHNLHTELFTTTTRLPPYATRWP PRTPGPGTSGTRRPPPPATLPPESDIDLGPRAYDRFPGDSRDYSTELSVTVAVGASLLFLNILAFAALYY KRDRRQELRCRRLSPPGGSGSGVPGGGPLLPTAGRELPPEEELVSLQLKRGGGVGADPAEALRPACPPDY TLALRRAPDDVPLLAPGALTLLPSGLGPPPPPPPPSLHPFGPFPPPPPTATSHNNTLPHPHSTTRVQA NNNNEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-HindIII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198862 |
| ORF Size | 2515 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198862.2, NP_942562.2 |
| RefSeq Size | 5034 bp |
| RefSeq ORF | 2511 bp |
| Locus ID | 216856 |
| UniProt ID | Q69ZK9 |
| MW | 91.4 kDa |
| Gene Summary | Transmembrane scaffolding protein involved in cell-cell interactions via its interactions with neurexin family members. Mediates cell-cell interactions both in neurons and in other types of cells, such as Langerhans beta cells. Mediates cell-cell interactions between Langerhans beta cells and modulates insulin secretion (By similarity). Plays a role in synapse function and synaptic signal transmission, especially via gamma-aminobutyric acid receptors (GABA(A) receptors). Functions by recruiting and clustering synaptic proteins. Promotes clustering of postsynaptic GABRG2 and GPHN. Promotes clustering of postsynaptic LHFPL4 (PubMed:29742426). Modulates signaling by inhibitory synapses, and thereby plays a role in controlling the ratio of signaling by excitatory and inhibitory synapses and information processing. Required for normal signal amplitude from inhibitory synapses, but is not essential for normal signal frequency. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221962 | Nlgn2 (untagged) - Mouse neuroligin 2 (Nlgn2), (10ug) |
CNY 10,640.00 |
|
| MG222168 | Nlgn2 (tGFP-tagged) - Mouse neuroligin 2 (Nlgn2), (10ug) |
CNY 7,744.00 |
|
| MR222168L3 | Lenti ORF clone of Nlgn2 (Myc-DDK-tagged) - Mouse neuroligin 2 (Nlgn2) |
CNY 9,030.00 |
|
| MR222168L4 | Lenti ORF clone of Nlgn2 (mGFP-tagged) - Mouse neuroligin 2 (Nlgn2) |
CNY 9,030.00 |
