Dvl3 (NM_007889) Mouse Tagged ORF Clone

CAT＃: MR222241

TrueORF^®

Dvl3 (Myc-DDK-tagged) - Mouse dishevelled 3, dsh homolog (Drosophila) (Dvl3)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_007889" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,248.00

CNY 6,270.00

货期*

现货

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	b2b2866Clo
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR222241 representing NM_007889 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGAGACCAAGATCATCTACCACCTGGACGGCCAGGAGACGCCGTACCTGGTGAAGCTGCCCCTGC CCGCCGAGCGCGTCACCTTGGCGGACTTTAAGGGCGTTCTGCAGCGGCCCAGCTATAAGTTCTTCTTCAA GTCTATGGACGACGATTTTGGGGTGGTGAAGGAAGAGATCTCGGACGACAATGCCAAGCTACCCTGCTTC AATGGCCGGGTGGTGTCCTGGCTGGTATCTGCCGAGGGCTCACACCCAGAGCCGGCCCCTTTCTGTGCTG ACAACCCGTCAGAGCTGCCACCTTCGATGGAACGCACAGGAGGCATTGGGGACTCTCGACCCCCCTCCTT CCACCCTCATGCTAGTGGGGGCAGCCAGGAGAATCTGGACAATGACACGGAGACCGACTCCTTGGTGTCT GCCCAACGAGAGCGGCCCCGACGGAGGGATGGCCCGGAGCACGCAGCCCGACTCAATGGAACCACAAAGG GTGAGCGGCGACGGGAGCCAGGCGGCTATGACAGTTCATCTACCCTCATGAGCAGCGAGCTGGAGACTAC CAGTTTCTTTGATTCCGATGAGGATGATTCCACCAGCAGGTTCAGCAGCTCCACAGAACAGAGCAGTGCC TCACGCCTCATGAGAAGACACAAGCGGCGGCGGCGGAAGCAGAAGGTCTCCCGGATTGAGCGGTCCTCGT CTTTCAGCAGCATCACAGACTCCACTATGTCTCTCAACATCATCACCGTCACTCTCAACATGGAAAAGTA TAACTTCTTGGGCATCTCCATTGTGGGCCAAAGCAATGAGCGAGGTGATGGGGGCATCTACATTGGCTCC ATCATGAAGGGTGGGGCTGTGGCAGCTGATGGACGCATTGAGCCAGGAGATATGCTACTGCAGGTAAATG AGATCAACTTCGAGAACATGAGCAACGATGACGCAGTCCGAGTCCTTCGGGAGATTGTGCACAAGCCAGG GCCCATCACTCTGACTGTAGCCAAGTGCTGGGACCCAAGTCCTCGCGGCTGCTTCACATTACCCCGGAGC GAGCCCATCCGTCCCATTGACCCTGCAGCCTGGGTCTCTCACACGGCAGCCATGACAGGCACCTTCCCCG CCTATGGCATGAGCCCCTCCTTAAGCACCATCACCTCTACCAGCTCCTCCATCACCAGCTCCATCCCTGA TACAGAGCGCCTAGATGATTTCCACCTCTCCATCCACAGTGACATGGCGGCCATCGTAAAAGCCATGGCC TCCCCTGAGTCAGGGTTGGAGGTCCGAGACCGCATGTGGCTCAAGATTACCATTCCCAATGCTTTCATCG GCTCGGATGTGGTGGACTGGCTGTACCACAACGTGGAAGGGTTCACCGACCGTCGGGAGGCCCGCAAGTA TGCTAGCAACCTGCTGAAAGCTGGTTTCATCCGCCATACCGTCAACAAGATCACGTTCTCCGAGCAGTGC TACTACATCTTTGGCGACCTCTGTGGTAACATGGCCAACCTATCGCTTCACGACCATGATGGCTCCAGTG GCGCCTCTGACCAGGACACACTGGCTCCTTTGCCGCACCCAGGAGCTGCCCCTTGGCCTATGGCTTTCCC TTACCAGTACCCACCACCTCCGCATCCCTACAACCCACACCCAGGCTTCCCAGAACTGGGCTACAGCTAC GGTGGGGGCAGCGCCAGCAGTCAGCACAGTGAAGGCAGTCGGAGCAGTGGCTCCAACCGAAGTGGCAGCG ACCGGCGCAAGGAGAAGGACCCAAAAGCTGGGGACTCCAAGTCAGGTGGTAGCGGCAGCGAGTCAGACCA CACTACACGCAGCAGTCTGAGGGGGCCACGGGAGCGGGCACCAAGCGAGCGATCAGGCCCAGCGGCCAGC GAGCACAGCCACCGCAGTCACCATTCGCTGACCAGCAGCCTGCGCAGCCACCACACACACCCAAGCTATG GCCCACCCGGAGTGCCCCCTCTCTATGGTCCCCCGATGCTGATGATGACCCCGCCGCCGGCAGCCATGGG TCCCCCAGGAGCCCCTCCAGGGCGTGACCTGGCCTCGGTGCCCCCGGAACTGACGGCCAGCAGACAGTCC TTCCGCATGGCAATGGGAAACCCCAGTGAGTTCTTTGTGGATGTGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR222241 representing NM_007889 Red=Cloning site Green=Tags(s) MGETKIIYHLDGQETPYLVKLPLPAERVTLADFKGVLQRPSYKFFFKSMDDDFGVVKEEISDDNAKLPCF NGRVVSWLVSAEGSHPEPAPFCADNPSELPPSMERTGGIGDSRPPSFHPHASGGSQENLDNDTETDSLVS AQRERPRRRDGPEHAARLNGTTKGERRREPGGYDSSSTLMSSELETTSFFDSDEDDSTSRFSSSTEQSSA SRLMRRHKRRRRKQKVSRIERSSSFSSITDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGS IMKGGAVAADGRIEPGDMLLQVNEINFENMSNDDAVRVLREIVHKPGPITLTVAKCWDPSPRGCFTLPRS EPIRPIDPAAWVSHTAAMTGTFPAYGMSPSLSTITSTSSSITSSIPDTERLDDFHLSIHSDMAAIVKAMA SPESGLEVRDRMWLKITIPNAFIGSDVVDWLYHNVEGFTDRREARKYASNLLKAGFIRHTVNKITFSEQC YYIFGDLCGNMANLSLHDHDGSSGASDQDTLAPLPHPGAAPWPMAFPYQYPPPPHPYNPHPGFPELGYSY GGGSASSQHSEGSRSSGSNRSGSDRRKEKDPKAGDSKSGGSGSESDHTTRSSLRGPRERAPSERSGPAAS EHSHRSHHSLTSSLRSHHTHPSYGPPGVPPLYGPPMLMMTPPPAAMGPPGAPPGRDLASVPPELTASRQS FRMAMGNPSEFFVDVM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_007889
ORF Size	2148 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_007889.3
RefSeq Size	2953 bp
RefSeq ORF	2151 bp
Locus ID	13544
UniProt ID	Q61062
MW	78.6 kDa
Gene Summary	Involved in the signal transduction pathway mediated by multiple Wnt genes.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC220985	Dvl3 (untagged) - Mouse dishevelled 3, dsh homolog (Drosophila) (Dvl3), (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,220.00
MG222241	Dvl3 (tGFP-tagged) - Mouse dishevelled 3 dsh homolog (Drosophila) (Dvl3), (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,940.00
MR222241L3	Lenti ORF clone of Dvl3 (Myc-DDK-tagged) - Mouse dishevelled 3, dsh homolog (Drosophila) (Dvl3)	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,170.00
MR222241L4	Lenti ORF clone of Dvl3 (mGFP-tagged) - Mouse dishevelled 3, dsh homolog (Drosophila) (Dvl3)	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,170.00

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Empty Vector

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