Klrg1 (NM_016970) Mouse Tagged ORF Clone

CAT#: MR222276

  • TrueORF®

Klrg1 (Myc-DDK-tagged) - Mouse killer cell lectin-like receptor subfamily G, member 1 (Klrg1)

ORF Plasmid: DDK tGFP



  "NM_016970" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2F1-Ag; MAFA; MAFA-L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR222276 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGACAGCTCTATCTATTCAACACTAGAGCTGCCGGAGGCACCTCAAGTCCAAGATGAGTCCAGAT
GGAAGCTCAAAGCTGTCTTACACCGGCCCCATCTTTCCCGCTTTGCAATGGTGGCTTTGGGGCTTTTGAC
TGTGATTCTCATGAGTCTACTGATGTATCAACGGATCCTGTGCTGCGGCTCCAAGGACTCTACATGTTCC
CACTGCCCCAGCTGCCCCATCCTCTGGACGAGGAATGGTAGCCACTGTTACTATTTTTCAATGGAGAAAA
AGGACTGGAATTCTAGTCTGAAATTCTGTGCAGACAAAGGCTCACATCTCCTTACATTTCCGGACAACCA
GGGAGTGCTGTTTGGAGAGTACCTGGGTCAGGACTTTTACTGGATCGGCTTGAGGAACATTGATGGCTGG
AGGTGGGAAGGCGGCCCAGCGCTCAGCTTGAGGATTCTTACCAACAGCTTGATACAGAGGTGCGGTGCCA
TTCACAGAAATGGCCTCCAAGCCTCCAGTTGTGAAGTTGCTTTGCAGTGGATCTGTAAGAAGGTCCTATA
C


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR222276 protein sequence
Red=Cloning site Green=Tags(s)

MADSSIYSTLELPEAPQVQDESRWKLKAVLHRPHLSRFAMVALGLLTVILMSLLMYQRILCCGSKDSTCS
HCPSCPILWTRNGSHCYYFSMEKKDWNSSLKFCADKGSHLLTFPDNQGVLFGEYLGQDFYWIGLRNIDGW
RWEGGPALSLRILTNSLIQRCGAIHRNGLQASSCEVALQWICKKVLY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016970
ORF Size 564 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016970.1, NP_058666.1
RefSeq Size 1374 bp
RefSeq ORF 567 bp
Locus ID 50928
UniProt ID O88713
MW 21.3 kDa
Gene Summary Plays an inhibitory role on natural killer (NK) cells and T-cell functions upon binding to their non-MHC ligands. May mediate missing self recognition by binding to a highly conserved site on classical cadherins, enabling it to monitor expression of E-cadherin/CDH1, N-cadherin/CDH2 and R-cadherin/CDH4 on target cells.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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