Adra2a (NM_007417) Mouse Tagged ORF Clone

CAT#: MR222562

  • TrueORF®

Adra2a (Myc-DDK-tagged) - Mouse adrenergic receptor, alpha 2a (Adra2a)

ORF Plasmid: DDK tGFP



  "NM_007417" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Adra-2; Adra-2a; alpha(2A)AR; alpha2-C10; alpha2A; alpha2A-AR; AW122659
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR222562 representing NM_007417
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCGCCAGGAGCAGCCGCTGGCCGAGGGCAGCTTTGCGCCCATGGGCTCACTGCAGCCGGATGCCG
GCAACAGCAGCTGGAACGGGACCGAAGCGCCCGGAGGCGGCACCCGAGCCACCCCTTACTCCCTGCAGGT
GACACTGACGCTGGTTTGCCTGGCTGGCCTGCTCATGCTGTTCACAGTATTTGGCAACGTGCTGGTTATT
ATCGCGGTGTTCACCAGTCGCGCGCTCAAAGCTCCCCAAAACCTCTTCCTGGTGTCCCTGGCCTCAGCGG
ACATCCTGGTGGCCACGCTGGTCATTCCCTTTTCTTTGGCCAACGAGGTTATGGGTTACTGGTACTTTGG
TAAGGTGTGGTGTGAGATCTATTTGGCTCTCGACGTGCTCTTTTGCACGTCGTCCATAGTGCACCTGTGC
GCCATCAGCCTTGACCGCTACTGGTCCATCACGCAGGCCATCGAGTACAACCTGAAGCGCACGCCGCGTC
GCATCAAGGCCATCATTGTCACCGTGTGGGTCATCTCGGCTGTCATCTCCTTCCCGCCACTCATCTCCAT
AGAGAAGAAGGGCGCTGGCGGCGGGCAGCAGCCGGCCGAGCCAAGCTGCAAGATCAACGACCAGAAGTGG
TATGTCATCTCCTCGTCCATCGGTTCCTTCTTCGCGCCTTGCCTCATCATGATCCTGGTCTACGTGCGTA
TTTACCAGATCGCCAAGCGTCGCACCCGCGTGCCTCCCAGCCGCCGGGGTCCGGACGCCTGTTCCGCGCC
GCCGGGGGGCGCCGATCGCAGGCCCAACGGGCTGGGCCCGGAGCGCGGCGCGGGTCCCACGGGCGCTGAG
GCGGAGCCGCTGCCCACCCAGCTTAACGGTGCCCCGGGGGAGCCCGCGCCCGCCGGGCCCCGCGATGGGG
ATGCGCTGGACCTAGAGGAGAGTTCGTCGTCCGAGCACGCCGAGCGGCCCCCGGGGCCCCGCAGACCCGA
CCGCGGCCCCCGAGCCAAGGGCAAGACCCGGGCGAGTCAGGTGAAGCCGGGGGACAGTCTGCCGCGGCGC
GGGCCCGGGGCCGCGGGGCCGGGGGCTTCGGGGTCCGGGCACGGAGAGGAGCGCGGCGGGGGCGCCAAAG
CGTCGCGCTGGCGCGGGAGGCAAAACCGGGAGAAACGCTTCACGTTCGTGCTGGCGGTGGTGATCGGCGT
GTTCGTGGTGTGTTGGTTTCCGTTCTTTTTCACCTACACGCTCATAGCGGTCGGCTGCCCGGTGCCCAGC
CAGCTCTTCAACTTCTTCTTCTGGTTCGGCTACTGCAACAGCTCGCTGAACCCTGTTATCTACACCATCT
TCAACCACGACTTCCGACGCGCCTTCAAGAAGATCCTCTGCCGTGGGGACAGAAAACGCATCGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR222562 representing NM_007417
Red=Cloning site Green=Tags(s)

MFRQEQPLAEGSFAPMGSLQPDAGNSSWNGTEAPGGGTRATPYSLQVTLTLVCLAGLLMLFTVFGNVLVI
IAVFTSRALKAPQNLFLVSLASADILVATLVIPFSLANEVMGYWYFGKVWCEIYLALDVLFCTSSIVHLC
AISLDRYWSITQAIEYNLKRTPRRIKAIIVTVWVISAVISFPPLISIEKKGAGGGQQPAEPSCKINDQKW
YVISSSIGSFFAPCLIMILVYVRIYQIAKRRTRVPPSRRGPDACSAPPGGADRRPNGLGPERGAGPTGAE
AEPLPTQLNGAPGEPAPAGPRDGDALDLEESSSSEHAERPPGPRRPDRGPRAKGKTRASQVKPGDSLPRR
GPGAAGPGASGSGHGEERGGGAKASRWRGRQNREKRFTFVLAVVIGVFVVCWFPFFFTYTLIAVGCPVPS
QLFNFFFWFGYCNSSLNPVIYTIFNHDFRRAFKKILCRGDRKRIV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_007417
ORF Size 1395 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007417.5
RefSeq Size 3818 bp
RefSeq ORF 1398 bp
Locus ID 11551
UniProt ID Q01338
MW 51 kDa
Gene Summary Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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