Sufu (NM_015752) Mouse Tagged ORF Clone
CAT#: MR222908
- TrueORF®
Sufu (Myc-DDK-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 1
ORF Plasmid: tGFP
"NM_015752" in other vectors (4)
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CNY 4,850.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Su(fu) |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR222908 representing NM_015752
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCTGCGGCCTAGCGTCGCCCCCGGTCCCGCCGCGCCCCCGGCCTCTGGCCCTAGTGCCCCTC CGGCCTTTGCTTCACTCTTTCCCCCGGGACTGCACGCCATCTACGGAGAGTGTCGCCGCCTCTACCCTGA CCAGCCGAACCCGCTCCAGGTTACCGCTATCGTCAAGTACTGGTTGGGTGGTCCGGACCCCTTGGACTAT GTTAGCATGTACAGGAACATGGGGAGTCCTTCTGCCAACATCCCTGAGCACTGGCACTACATCAGCTTTG GCCTGAGTGATCTCTATGGTGACAACAGAGTCCATGAGTTTACAGGAACAGACGGACCAAGTGGATTTGG CTTTGAGTTGACGTTTCGTCTGAAGAGAGAAACTGGGGAGTCTGCCCCACCAACATGGCCAGCAGAGCTG ATGCAGGGCCTAGCCCGATATGTCTTCCAGTCAGAGAACACCTTCTGTAGCGGGGACCATGTGTCTTGGC ACAGCCCTTTGGATAACAGTGAGTCAAGAATTCAGCACATGCTGCTGACGGAGGACCCACAGATGCAGCC TGTGCGGACACCCTTTGGGGTAGTGACTTTCCTCCAGATTGTTGGTGTCTGCACTGAGGAGTTACATTCA GCCCAACAGTGGAACGGGCAGGGCATCCTGGAACTACTACGGACAGTGCCCATTGCTGGCGGTCCCTGGC TGATAACTGACATGCGGCGGGGAGAAACCATATTTGAGATCGATCCGCACCTGCAAGAGAGAGTTGACAA AGGCATTGAGACAGACGGTTCTAACCTGAGCGGCGTCAGTGCCAAGTGTGCCTGGGATGACCTCAGCCGG CCTCCGGAGGATGAAGAGGATAGCCGGAGCATCTGCCTCGGCACACAGCCTCGGAGGCTGTCTGGCAAAG ACACAGAGCAGATCCGGGAGACCCTGAGGCGGGGACTGGAGATTAACAGCAAACCTGTCCTTCCACCAAT CAATTCTCAGCGACAGAACGGCCTCACCCACGACAGGGCTCCGAGCCGCAAGGACAGTTTGGGCAGCGAC AGCTCCACGGCCATCATCCCCCACGAGCTGATCCGCACACGGCAGCTCGAGAGCGTGCATCTAAAATTTA ACCAAGAGTCGGGAGCCCTCATCCCTCTCTGCCTAAGGGGCAGACTCCTACATGGCCGGCACTTCACCTA CAAGAGTATCACAGGCGACATGGCCATCACGTTTGTGTCCACGGGAGTGGAAGGCGCCTTTGCCACTGAG GAACACCCGTATGCAGCTCACGGACCCTGGTTACAAATTCTGTTGACAGAAGAGTTTGTAGAGAAGATGT TGGAGGACTTAGAAGATCTAACCTCTCCAGAGGAATTTAAACTTCCCAAAGAGTACAGCTGGCCTGAGAA GAAACTCAAAGTGTCCATTCTCCCCGACGTGGTGTTCGACAGTCCACTGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222908 representing NM_015752
Red=Cloning site Green=Tags(s) MAELRPSVAPGPAAPPASGPSAPPAFASLFPPGLHAIYGECRRLYPDQPNPLQVTAIVKYWLGGPDPLDY VSMYRNMGSPSANIPEHWHYISFGLSDLYGDNRVHEFTGTDGPSGFGFELTFRLKRETGESAPPTWPAEL MQGLARYVFQSENTFCSGDHVSWHSPLDNSESRIQHMLLTEDPQMQPVRTPFGVVTFLQIVGVCTEELHS AQQWNGQGILELLRTVPIAGGPWLITDMRRGETIFEIDPHLQERVDKGIETDGSNLSGVSAKCAWDDLSR PPEDEEDSRSICLGTQPRRLSGKDTEQIRETLRRGLEINSKPVLPPINSQRQNGLTHDRAPSRKDSLGSD SSTAIIPHELIRTRQLESVHLKFNQESGALIPLCLRGRLLHGRHFTYKSITGDMAITFVSTGVEGAFATE EHPYAAHGPWLQILLTEEFVEKMLEDLEDLTSPEEFKLPKEYSWPEKKLKVSILPDVVFDSPLH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_015752 |
ORF Size | 1452 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015752.1, NM_015752.2, NM_015752.3, NP_056567.2 |
RefSeq Size | 2359 bp |
RefSeq ORF | 1458 bp |
Locus ID | 24069 |
UniProt ID | Q9Z0P7 |
MW | 54.5 kDa |
Gene Summary | Negative regulator in the hedgehog/smoothened signaling pathway (PubMed:16155214, PubMed:16459298). Down-regulates GLI1-mediated transactivation of target genes (PubMed:11960000). Part of a corepressor complex that acts on DNA-bound GLI1 (PubMed:11960000). May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome (By similarity). Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein (PubMed:10531011, PubMed:16459298). Negative regulator of beta-catenin signaling (PubMed:11477086). Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full-length form of GLI3 (GLI3FL) (PubMed:10531011, PubMed:20360384). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state (PubMed:10531011, PubMed:20360384). Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R (PubMed:10531011, PubMed:20360384). When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A) (PubMed:10531011, PubMed:20360384). Required for normal embryonic development (PubMed:16155214, PubMed:16459298). Required for the proper formation of hair follicles and the control of epidermal differentiation (PubMed:16155214, PubMed:16459298, PubMed:23034632).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216658 | Sufu (untagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 1, (10ug) |
CNY 6,270.00 |
|
MG222908 | Sufu (tGFP-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu) transcript variant 1, (10ug) |
CNY 5,320.00 |
|
MR222908L3 | Lenti ORF clone of Sufu (Myc-DDK-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 1 |
CNY 6,750.00 |
|
MR222908L4 | Lenti ORF clone of Sufu (mGFP-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 1 |
CNY 6,750.00 |