Emx1 (NM_010131) Mouse Tagged ORF Clone

CAT#: MR222943

  • TrueORF®

Emx1 (Myc-DDK-tagged) - Mouse empty spiracles homolog 1 (Drosophila) (Emx1)

ORF Plasmid: DDK tGFP



  "NM_010131" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,400.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Emx1 Antibody - middle region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms MGC129284; MGC129285
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR222943 representing NM_010131
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCAGCCCGCGGCTAAGCGGGGTTTCACCATAGAGTCCTTGGTGGCCAAGGATGGTGGCACCGGCG
GGAGTCCTGGCAGCGGGGGCGCGGGCTCCCATCCACTGGCTGTGGCCGCATCAGAGGAACCGCTCAGGCC
CACGGCGCTCAACTATCCTCACCCCAGTGCGGCCGAGACGGCCTTCGTGAGTGGCTTCCCTGCCGCTGCC
GCTGCGGGCGCCGGCCGCTCGCTCTATGGAGGGCCGGAGCTGGTGTTCCCAGAGGCCATGAACCACCCGG
CTCTGACGGTGCACCCGGCACACCAGCTGGGCTCCTCCTCGCTGCAACCCCCTCACTCTTTCTTCAGCGC
CCAGCATCGGGACCCTCTCCACTTCTACCCCTGGGTGCTTCGGAATCGCTTCTTTGGCCACCGCTTCCAG
GCAAGCGACGTTCCCCAGGACGGGCTGCTTTTGCACGGGCCCTTCGCACGCAAGCCCAAGCGGATTCGCA
CAGCCTTCTCGCCCTCGCAGCTGCTGCGGCTGGAGCGAGCCTTTGAGAAGAATCACTACGTGGTGGGAGC
CGAGCGGAAGCAGCTGGCAGGCAGCCTCAGCCTCTCCGAGACGCAGGTGAAGGTGTGGTTCCAGAATCGG
AGGACAAAATACAAACGGCAGAAGCTGGAAGAGGAAGGGCCGGAGTCTGAGCAGAAGAAGAAGGGTTCCC
ACCATATCAACCGGTGGCGCATCGCCACGAAGCAGGCCAACGGGGAGGACATTGATGTCACCTCCAATGA
C


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR222943 representing NM_010131
Red=Cloning site Green=Tags(s)

MFQPAAKRGFTIESLVAKDGGTGGSPGSGGAGSHPLAVAASEEPLRPTALNYPHPSAAETAFVSGFPAAA
AAGAGRSLYGGPELVFPEAMNHPALTVHPAHQLGSSSLQPPHSFFSAQHRDPLHFYPWVLRNRFFGHRFQ
ASDVPQDGLLLHGPFARKPKRIRTAFSPSQLLRLERAFEKNHYVVGAERKQLAGSLSLSETQVKVWFQNR
RTKYKRQKLEEEGPESEQKKKGSHHINRWRIATKQANGEDIDVTSND

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_010131
ORF Size 771 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_010131.2, NP_034261.1
RefSeq Size 1188 bp
RefSeq ORF 774 bp
Locus ID 13796
UniProt ID Q04742
MW 28.6 kDa
Gene Summary Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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