Col6a1 (NM_009933) Mouse Tagged ORF Clone
CAT#: MR223027
- TrueORF®
Col6a1 (Myc-DDK-tagged) - Mouse collagen, type VI, alpha 1 (Col6a1)
ORF Plasmid: tGFP
"NM_009933" in other vectors (4)
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CNY 7,136.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI747156; Col6a-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR223027 representing NM_009933
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTGGCCCACGCTCTGCTGCCCCTGCTGCTACAAGCCTGCTGGGTGGCCACACAGGACATCCAGG GCTCCAAAGCGATTGCCTTCCAAGACTGCCCTGTGGATCTATTCTTCGTGCTCGACACCTCGGAGAGTGT GGCCTTGAGGCTGAAACCTTATGGGGCCTTGGTGGACAAGGTGAAGTCCTTCACTAAGCGCTTCATTGAC AACCTGAGAGACAGGTACTACCGGTGTGACCGCAACCTGGTTTGGAATGCGGGTGCGCTGCACTACAGTG ACGAGGTGGAGATCATCCGAGGGCTCACGCGCATGCCCAGTGGCCGCGATGAGCTCAAGGCCAGCGTGGA TGCGGTCAAGTACTTCGGGAAAGGCACCTACACCGACTGCGCCATTAAGAAGGGGCTGGAGGAGCTGCTC ATAGGGGGCTCCCACCTGAAGGAGAACAAGTACTTGATCGTGGTGACCGACGGGCATCCTCTAGAGGGCT ACAAGGAACCATGCGGGGGTCTGGAAGATGCAGTAAATGAGGCCAAACACCTGGGCATCAAGGTCTTTTC TGTGGCCATCACACCTGACCACCTGGAGCCACGTCTAAGTATCATTGCCACAGACCACACATACCGGCGC AATTTCACGGCAGCTGACTGGGGGCATAGCCGCGATGCAGAAGAGGTCATCAGCCAGACCATTGACACCA TTGTGGACATGATTAAAAATAACGTGGAACAAGTGTGTTGTTCTTTTGAGTGCCAGGCTGCCAGAGGACC TCCAGGGCCCCGAGGCGACCCTGGGTATGAGGGGGAGCGAGGAAAGCCAGGTCTTCCGGGAGAGAAGGGA GAAGCTGGAGACCCTGGACGACCTGGGGATCTTGGACCAGTCGGGTACCAGGGTATGAAGGGAGAAAAGG GGAGCCGTGGAGAGAAGGGTTCCAGAGGACCGAAAGGTTACAAGGGCGAGAAAGGCAAGCGCGGAATCGA CGGGGTCGACGGCATGAAGGGAGAGACGGGGTACCCAGGACTACCGGGCTGCAAGGGCTCCCCAGGATTT GATGGCATTCAAGGACCCCCGGGTCCCAAGGGTGATGCTGGTGCCTTTGGGATGAAGGGAGAAAAGGGTG AAGCTGGAGCAGACGGTGAGGCTGGGAGACCAGGGAACTCAGGGTCACCTGGAGATGAGGGTGATCCTGG AGAGCCTGGTCCCCCCGGAGAAAAAGGAGAGGCCGGTGATGAAGGAAATGCTGGCCCAGACGGTGCCCCT GGAGAGAGGGGTGGCCCTGGTGAAAGAGGACCTCGGGGGACCCCTGGTGTGAGAGGACCAAGGGGAGACC CGGGTGAAGCTGGACCACAGGGTGACCAAGGAAGAGAGGGGCCCGTCGGCATCCCTGGAGACTCGGGTGA GGCTGGCCCCATTGGACCTAAAGGATACCGAGGTGATGAGGGTCCTCCAGGTCCTGAGGGCCTCAGAGGA GCCCCAGGACCTGTTGGTCCTCCTGGAGACCCCGGACTGATGGGTGAGAGAGGTGAGGATGGACCACCAG GAAACGGCACGGAAGGTTTCCCCGGCTTCCCTGGGTATCCAGGCAACAGAGGCCCTCCTGGGCTAAATGG CACAAAAGGCTACCCTGGCCTCAAGGGGGATGAGGGTGAAGTGGGAGACCCAGGAGAGGATAACAACGAC ATTTCACCCCGTGGGGTCAAAGGGGCAAAGGGATACCGAGGCCCAGAAGGACCCCAGGGACCTCCAGGAC ATGTGGGACCACCTGGGCCAGATGAGTGTGAGATCCTGGATATCATCATGAAAATGTGCTCCTGCTGTGA GTGCACATGTGGACCCATTGACATCCTCTTCGTGCTGGACAGCTCGGAGAGCATTGGCCTACAGAACTTT GAGATTGCCAAGGACTTCATCATCAAGGTCATTGACCGGTTGAGCAAGGATGAGCTGGTCAAATTTGAGC CAGGGCAGTCTCACGCGGGCGTGGTACAGTACAGCCACAACCAGATGCAAGAGCACGTGGACATGCGGAG CCCCAACGTCCGCAACGCCCAGGACTTCAAAGAAGCTGTCAAGAAGCTACAATGGATGGCTGGTGGCACA TTCACCGGAGAAGCGCTGCAGTACACCCGGGACCGGCTACTCCCACCCACACAGAACAACCGAATTGCCC TGGTCATTACGGATGGACGTTCTGACACTCAACGGGACACGACACCTCTCAGTGTGCTCTGTGGTGCAGA CATTCAGGTAGTTTCTGTGGGAATCAAGGATGTGTTTGGCTTTGTGGCGGGCTCCGACCAGCTCAATGTC ATTTCCTGCCAAGGCTTATCGCAAGGTCGGCCAGGTATCTCCCTGGTGAAGGAGAACTATGCAGAGCTTC TCGATGACGGCTTTCTGAAGAACATAACAGCCCAGATCTGTATAGATAAGAAGTGTCCGGATTATACCTG TCCAATCACATTCTCCTCCCCGGCTGACATCACCATCCTGCTAGACAGCTCAGCCAGTGTCGGCAGCCAC AACTTCGAAACCACCAAGGTCTTCGCCAAGCGCCTAGCTGAGCGATTCCTGTCAGCAGGCAGGGCGGATC CTTCCCAGGATGTGCGGGTGGCCGTGGTACAGTATAGTGGCCAGGGGCAGCAACAGCCAGGTCGGGCGGC TCTTCAGTTCTTACAGAATTACACAGTGCTGGCCAGCTCTGTGGACAGCATGGATTTCATCAACGACGCC ACAGACGTCAACGATGCTCTGAGCTACGTGACTCGTTTCTACCGGGAAGCCTCGTCAGGTGCCACCAAGA AGAGAGTGCTGTTGTTTTCAGACGGCAACTCTCAGGGGGCCACAGCAGAGGCCATTGAGAAGGCTGTGCA GGAGGCCCAGCGTGCAGGCATTGAGATCTTTGTGGTGGTGGTGGGACCCCAGGTGAACGAGCCCCACATC CGTGTGCTTGTCACTGGCAAGACTGCAGAGTACGACGTGGCCTTTGGCGAGCGCCACCTATTCCGTGTAC CAAACTACCAGGCCCTGCTACGTGGCGTACTCTACCAGACAGTCTCCAGGAAGGTGGCACTGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223027 representing NM_009933
Red=Cloning site Green=Tags(s) MRLAHALLPLLLQACWVATQDIQGSKAIAFQDCPVDLFFVLDTSESVALRLKPYGALVDKVKSFTKRFID NLRDRYYRCDRNLVWNAGALHYSDEVEIIRGLTRMPSGRDELKASVDAVKYFGKGTYTDCAIKKGLEELL IGGSHLKENKYLIVVTDGHPLEGYKEPCGGLEDAVNEAKHLGIKVFSVAITPDHLEPRLSIIATDHTYRR NFTAADWGHSRDAEEVISQTIDTIVDMIKNNVEQVCCSFECQAARGPPGPRGDPGYEGERGKPGLPGEKG EAGDPGRPGDLGPVGYQGMKGEKGSRGEKGSRGPKGYKGEKGKRGIDGVDGMKGETGYPGLPGCKGSPGF DGIQGPPGPKGDAGAFGMKGEKGEAGADGEAGRPGNSGSPGDEGDPGEPGPPGEKGEAGDEGNAGPDGAP GERGGPGERGPRGTPGVRGPRGDPGEAGPQGDQGREGPVGIPGDSGEAGPIGPKGYRGDEGPPGPEGLRG APGPVGPPGDPGLMGERGEDGPPGNGTEGFPGFPGYPGNRGPPGLNGTKGYPGLKGDEGEVGDPGEDNND ISPRGVKGAKGYRGPEGPQGPPGHVGPPGPDECEILDIIMKMCSCCECTCGPIDILFVLDSSESIGLQNF EIAKDFIIKVIDRLSKDELVKFEPGQSHAGVVQYSHNQMQEHVDMRSPNVRNAQDFKEAVKKLQWMAGGT FTGEALQYTRDRLLPPTQNNRIALVITDGRSDTQRDTTPLSVLCGADIQVVSVGIKDVFGFVAGSDQLNV ISCQGLSQGRPGISLVKENYAELLDDGFLKNITAQICIDKKCPDYTCPITFSSPADITILLDSSASVGSH NFETTKVFAKRLAERFLSAGRADPSQDVRVAVVQYSGQGQQQPGRAALQFLQNYTVLASSVDSMDFINDA TDVNDALSYVTRFYREASSGATKKRVLLFSDGNSQGATAEAIEKAVQEAQRAGIEIFVVVVGPQVNEPHI RVLVTGKTAEYDVAFGERHLFRVPNYQALLRGVLYQTVSRKVALG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009933 |
ORF Size | 3075 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009933.4, NP_034063.1 |
RefSeq Size | 3990 bp |
RefSeq ORF | 3078 bp |
Locus ID | 12833 |
UniProt ID | Q04857 |
MW | 108.5 kDa |
Gene Summary | Collagen VI acts as a cell-binding protein.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223231 | Col6a1 (untagged) - Mouse collagen, type VI, alpha 1 (Col6a1), (10ug) |
CNY 13,210.00 |
|
MG223027 | Col6a1 (tGFP-tagged) - Mouse collagen type VI alpha 1 (Col6a1), (10ug) |
CNY 8,736.00 |
|
MR223027L3 | Lenti ORF clone of Col6a1 (Myc-DDK-tagged) - Mouse collagen, type VI, alpha 1 (Col6a1) |
CNY 10,830.00 |
|
MR223027L4 | Lenti ORF clone of Col6a1 (mGFP-tagged) - Mouse collagen, type VI, alpha 1 (Col6a1) |
CNY 10,830.00 |