Hltf (NM_144959) Mouse Tagged Lenti ORF Clone
CAT#: MR223364L4
- LentiORF®
Lenti ORF clone of Hltf (mGFP-tagged) - Mouse helicase-like transcription factor (Hltf), transcript variant 2
"NM_144959" in other vectors (4)
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CNY 10,260.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Synonyms | AF010600; BC057116; P113; Smarca3; Snf2l3 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR223364).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_144959 |
ORF Size | 2523 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_144959.2, NP_659208.2 |
RefSeq Size | 3059 bp |
RefSeq ORF | 2526 bp |
Locus ID | 20585 |
Gene Summary | Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity. This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222000 | Hltf (untagged) - Mouse helicase-like transcription factor (Hltf), transcript variant 2, (10ug) |
CNY 10,740.00 |
|
MG223364 | Hltf (tGFP-tagged) - Mouse helicase-like transcription factor (Hltf) transcript variant 2, (10ug) |
CNY 9,220.00 |
|
MR223364 | Hltf (Myc-DDK-tagged) - Mouse helicase-like transcription factor (Hltf), transcript variant 2 |
CNY 8,360.00 |
|
MR223364L3 | Lenti ORF clone of Hltf (Myc-DDK-tagged) - Mouse helicase-like transcription factor (Hltf), transcript variant 2 |
CNY 10,260.00 |