Kmt2e (NM_026984) Mouse Tagged ORF Clone
CAT#: MR223799
- TrueORF®
Mll5 (Myc-DDK-tagged) - Mouse myeloid/lymphoid or mixed-lineage leukemia 5 (Mll5). Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_026984" in other vectors (2)
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CNY 13,816.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1810033J14Rik; 9530077A04Rik; D230038D11Rik; Mll5; NKp44L |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR223799 representing NM_026984, codon optimized.
Due to the complexity of NM_026984, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATAGCGATCCCATTGGGGGTTGATACAACAGAAACATCCTACTTGGAAATGGCTGCAGGCTCAG AACCGGAATCCGTAGAAGCTAGCCCTGTGGTAGTTGAGAAATCCAACAGTTTTCCCCACCAGTTATACAC CAGCAGCTCCCACCACTCACACAGCTACATCGGTCTGCCCTATGCGGACCATAATTATGGTGCTCGTCCT CCTCCAACACCTCCAGCATCCCCTCCTCCATCAGGTCTCATTAGCAAAAACGAAGTAGGCATATTTACCA CTCCTAATTTTGATGAAACTTCCAGTGCTACTACAATCAGCACATCTGAAGATGGAAGTTACGGTACTGA TGTAACCAGGTGCATATGTGGTTTTACACATGATGACGGATACATGATCTGTTGTGACAAATGCAGTGTT TGGCAACATATTGACTGCATGGGGATTGACAGGCAGCATATTCCTGATACATACCTATGTGAACGTTGTC AGCCCAGGAGTTTGGATAAAGAGAGGGCAGTGCTACTGCAACGCCGGAAAAGAGAAAATATGTCAGATGG TGATACCAGTGCAACTGAAAGTGGTGATGAAGTTCCTGTGGAATTATATACTGCATTTCAGCATACTCCA ACTTCAATCACTTTAACTGCTTCCAGAGTTCCCAAGGTTACTGATAAAAGAAGGAAAAAAAGTGGGGAGA AAGAACAAAACTTTTCAAAATGTAAAAAAGCCTTTCGTGAAGGATCTAGGAAATCATCAAGGGTTAAGGG TTCAGCACCAGAAATTGATCCTTCATCTGATAGTTCAAATTTTGTTTGGGAAACAAAAATCAAAGCATGG ATGGATCGTTACGAAGAAGCAAATAATAACCAATATAGTGAAGGTGTTCAGAGAGAGGCACAAAGACTAG CTCAAAGACTCGGTAGTGGGAATGACAGCAAAGACATGAATAAATCAGAATTGAGTACCAACAACTCACT CTTCAGACCTCCTGTAGAGAGCCATATACAGAAGAATAAAAAAATTCTGAAATCTGCCAAAGACTTGCCT CCTGATGCACTTATCATTGAATATAGAGGGAAGTTTATGCTGAGAGAACAGTTTGAAGCAAATGGATATT TCTTTAAAAGGCCATACCCTTTTGTTTTATTCTACTCTAAGTTCCATGGGCTTGAAATGTGTGTTGATGC GAGGACTTTTGGGAATGAGGCTCGATTCATCAGGCGTTCTTGTACACCGAATGCAGAGGTGAGGCATGAA ATTGAAGAGGGGACCATACATCTCTACATTTATTCTATACAGAGTATTCCAAAAGGGACTGAAATTACCA TTGCCTTTGATTTTGACTACGGGAATTGCAAATACAAGGTGGACTGTGCATGCCTCAAGGAAAATCCAGA GTGCCCTGTTCTCAAGCGAAGCTCGGAATCCACAGAAAACATCAATAGTGGTTATGAGACCAGAAGGAAA AAAGGAAAAAAAGAGAAAGATACTTCAAAAGAAAAAGACATACAAAATCAGAATATGACTTTGGACTGTG AAGGAACAAACAACAAAATAAGGAGTCCAGAAACAAAACAGAGAAAGCTTTCTCCATTGAGGCTGTCGGT GTCAAACAACCAGGAACCAGATTTTATTGATGATATGGAAGAAAAAACTCCTATTAGCAATGAAGTGGAA ATGGAATCTGAGGAACAGATTGCAGAAAGGAAAAGGAAGATGACAAGAGAAGAAAGAAAAATGGAAGCAA TTTTGCAGGCTTTTGCCAGACTTGAGAAGAGAGAGAAAAGACGAGAACAAGCTTTGGAAAGGATCAGCAC AGCTAAGACAGAAGTTAAGCCTGAATGCAAAGAGTCGCAGGTCATCGCCGATGCCGAAGTGGTGCAGGAA CAAGTAAAAGAAGAAACTGCTATCAAGCCAGCCGCTGCCAAAGTGAACAGAACTAAGCAGAGAAAGAGCT TTTCTCGTAGTAGGACTCACATTGGACAGCAGCGTCGGAGACACAGAACTGTCAGCATGTGTTCAGACAT ACCGCCATCTTCTCCTGACATCGAAGTTCTGTCACAACAGAACGAGATTGAAAATACTGTACTTGCTATA GAACCAGAAACTGAAACTGCAGTAGCAGAAATAATTCCTGAAGCGGAAGTTCCAGCACTTAATAAGTGTC CCACAAAGTACCCCAAAACAAAGAAGCACTTGGTCAATGAATGGTTAAGTGAGAAGAATGAGAAGACAGG AAAACCTTCAGACAGCCTTTCAGAAAGGCCTCTGCGCATAACTACAGATCCAGAGGTGTTAGCTACACAG CTCAATTCTTTGCCAGGTCTCACTTACAGCCCCCATGTATACTCTACTCCTAAGCATTATATTAGATTTA CATCACCATTCCTTTCGGAAAAAAAGAGAAGAAAAGAAACTACTGAAAACATTTCTGGCTCATGCAAAAA GCGGTGGCTGAAGCAAGCCCTGGAGGAAGAAAATTCAACAATTTTACATAGATATCATTCACCTTGTCAG GAAAGATCCAGGAGTCCTACAGTTAATGGTGAAAACAAAAGTCCACTTCTGCTAAGCGACAGCTGCTCCC TCCCAGATTTAACCACACCACTAAAAAAGCGAAGACTTTACCAATTGTTAGATACTGCTTACTCAGAAAG CTCCACTCCTACCCCTTCACCATATGCTACACCAACTCACACAGACATCACTCCTACGGACCCAGCATTT GCCACTCCCCCACGGATCAAATCAGATGATGAAACTTACAGAAATGGTTATAAACCCATATATTCACCAG TGACCCCTGTAACCCCTGGCACACCAGGAAACACCATGCACTTTGAGAATATTTCTTCCCCAGAAAGTTC TCCAGAAATAAAGAGATGCACTTACAATCAAGAGGGATATGACCGACCTTCAAACATGCTAACGTTGGGG CCTTTTAGAAATTCCAATTTAACTGAGCTGGGCCTGCAAGAAATAAAGACTATTGGTTATACCAGCCCTA GGAGCAGGACTGAGGTTAACAGGCCTTGCCCAGGGGAGAAGGAGTCTGTGTCAGACCTTCAACTGGGACT CGATGCAGTCGAGCCAGCTGCCTTACAGAAATCCATGGAAACACCAGCCCATGACAGGACTGAGCCCAGC AACCAACTGGATTCGACCCACTCTGGACGGGGCACAATGTATTCATCCTGGGTAAAGAGTCCTGACAGAA CAGGAGTTAACTTCTCAGTAAACTCCAACTTGAGGGACTTAACACCCTCTCATCAGTTGGAGACTGGAGG CGGCTTCCGAGTAAGTGAGTCCAAGTGCCTGATTCAGCAGGATGACACTAGAGGCATGTTTCTGGGAGCG GCTGTCTTTTGTACTTCTGAAGACGGGCTTGCCTCTGGTTTTGGACGGACTGTTAATGACAATTTGATCG ACGGGAGTTGCACGCCCCAGAATCCACCACAAAAGAAAAAGGTTTCTCTATTAGAATACCGTAAGAGACA ACGTGAAGCTAGGAAGAGTGGGTCTAAGCCAGAGAACTTTGCTTTGATTAGTGTGTCACCTCACCCGAGT GGGAGCCTGAGCAGCAGTGGCGACGGGTGTGTCCACAGCAGTGAAAACGGGGAGCAGGCAGAAAACCAGG CTAGCTTGCCTTTACCACCGCCTGCTGCCGCCGCCGCCGCCACCGCCGCCGCCGCTTACAGTGCCTCTTC TGAAGAAGGCAGCAGTAACTGTCCTGTTAAAGACGCCAATTCCAGTGAGAAGAAAGACCCAGAAGTTCAG TGGACTGCCTCCACTTCAGTGGAGCAAGTGAGAGAGAGAAGTTACCAGAGAGCCTTACTGCTCAGTGACC ACCGCAAAGACAAAGACAGTGGGGGAGAGTCACCATGTGTCTCATGCTCACCGAGTCATGTTCAGTCTCC ACCTTCATCTCATTCAAATCACATTCCCCAGGTGCACGCTCAGAGCCTAGCTCCTTCTTTGAGTGAACTC ATGGCAGACCCTGATGCGGAAGGTACAGAAGCCACAAGTACAAGTGAATGTCCATCCCCTGACACTTCTC AGAGCCCTTCTAAAACAAGCAAGCCTGGTTCACCAGGGCCAATCAATCCTGCTCAGTCACATGGGAAAAT ACTCACAAAACCAGATAGCCACTGGGAGGCAACTGCCACTGTATCAGAAGCTGACAACAGTGTTCACCAA AACCCAGAGCCTCAGCACAGACAGCTGTCAAGTAACACCCCAGCACTTTCTCAGAACCATGCTCCTCAGG CCCACGCTCTGAGTGCGAATGATCAACTTCCGCAAAAGCTGCCTTCTGCACCGACGAAGTTGCACTGTCC TCCGTCACCTCACACAGAAAACCCTCCCAAGTCCTCCACACCTCACACCCCCGTGCAGCATGGTTACCTC TCGCCAAAGCCTCCTTCACAGCACTTAGGATCTCCCTTCAGGCCTCACCATTCACAATCACCTCAAGTTG GAACTCCTCAACGAGAGACTCAGCGCAACTTTTACGCCGCAGCCCAGAATCTGCAGGCCAACCCCCAGCA GGCTACCTCAGGAGCTCTTTTTACTCAAACTCCTTCAGGGCAGTCATCCGCAACTTATTCCCAGTTCAAT CAGCAAAGCCTCAATAGTACGGCCCCGCCTCCACCTCCACCGCCGCCTCCTAGTAGTTATTATCAAAACC AGCAGCCAAGTGCTAATTTTCAGAACTATAACCAACTGAAGGGAAGCCTTAGCCAGCAGACGGTCTTCAC AAGCGGGCCTAATCAAGCCCTGCCTGGCTCTACTTCACAGCAGTCCGTGCCTGGGCACCACGTCACACCT GGGCACTTCCTGCCATCCCAGAACCCCACAATACACCATCAACCTGCCGCGGCCGCCGTCGTGCCACCTC CTCCTCCGCCCCCGCCCGCACCCGGCCCACATCTGATTCAGCAGCCAAGCAGTCATCAGCAGCACAGTGT AGCACATGGGGTGGGCCCAGTACATGCTGTGACACCCGGCTCCCACATTCATTCACAGACCGCCGGACAT CACCTGCCTCCCCCGCCTCCGCCCCCAGGCCCCGCACCACATCACCACCCACCCCCCCATCCGACGACCG GTCTGCAGAGCCTGCAAGCCCAGCATCAGCACGTCGTTAACTCCGCTCCACCTCCTCCACCTCCACCACC CCCACCCCCGCCCGCTAGCGTGTTGGTCAGCGGGCATCACTCCGCCAGTGGTCAGGCCCTGCACCACCCT CCACACCAGGGTCCTCCCCTCTTCCCTGCAAGCGCTCACCCAGCCGTCCCTCCATATCCAAGCCAGGCGA CCCACCACACCACCCTGGGCCCCGGCCCTCAACACCAGCCATCTGGCACTGGCCCTCACTGTCCTCTCCC CGTCGCAGGGCCACACTTGCAGCCTCAGGGGCCAAATAGCATACCTACTCCTACAGCTTCAGGTTTTTGT CCTCACCCCCACCCAGGTAGCGTGGCGCTGCCACACGGCGTGCAGGGTCCTCAGCAGGCCTCCCCTGTGC CCGCGCAGATTCCAATCCACAGGGCCCAAGTGCCTCCCACTTTCCAGAATAACTATCACGGTTCCGGATG GCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223799 representing NM_026984
Red=Cloning site Green=Tags(s) MSIAIPLGVDTTETSYLEMAAGSEPESVEASPVVVEKSNSFPHQLYTSSSHHSHSYIGLPYADHNYGARP PPTPPASPPPSGLISKNEVGIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSV WQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTP TSITLTASRVPKVTDKRRKKSGEKEQNFSKCKKAFREGSRKSSRVKGSAPEIDPSSDSSNFVWETKIKAW MDRYEEANNNQYSEGVQREAQRLAQRLGSGNDSKDMNKSELSTNNSLFRPPVESHIQKNKKILKSAKDLP PDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHE IEEGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENPECPVLKRSSESTENINSGYETRRK KGKKEKDTSKEKDIQNQNMTLDCEGTNNKIRSPETKQRKLSPLRLSVSNNQEPDFIDDMEEKTPISNEVE MESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKPECKESQVIADAEVVQE QVKEETAIKPAAAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIPPSSPDIEVLSQQNEIENTVLAI EPETETAVAEIIPEAEVPALNKCPTKYPKTKKHLVNEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQ LNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKKRRKETTENISGSCKKRWLKQALEEENSTILHRYHSPCQ ERSRSPTVNGENKSPLLLSDSCSLPDLTTPLKKRRLYQLLDTAYSESSTPTPSPYATPTHTDITPTDPAF ATPPRIKSDDETYRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRCTYNQEGYDRPSNMLTLG PFRNSNLTELGLQEIKTIGYTSPRSRTEVNRPCPGEKESVSDLQLGLDAVEPAALQKSMETPAHDRTEPS NQLDSTHSGRGTMYSSWVKSPDRTGVNFSVNSNLRDLTPSHQLETGGGFRVSESKCLIQQDDTRGMFLGA AVFCTSEDGLASGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKPENFALISVSPHPS GSLSSSGDGCVHSSENGEQAENQASLPLPPPAAAAAATAAAAYSASSEEGSSNCPVKDANSSEKKDPEVQ WTASTSVEQVRERSYQRALLLSDHRKDKDSGGESPCVSCSPSHVQSPPSSHSNHIPQVHAQSLAPSLSEL MADPDAEGTEATSTSECPSPDTSQSPSKTSKPGSPGPINPAQSHGKILTKPDSHWEATATVSEADNSVHQ NPEPQHRQLSSNTPALSQNHAPQAHALSANDQLPQKLPSAPTKLHCPPSPHTENPPKSSTPHTPVQHGYL SPKPPSQHLGSPFRPHHSQSPQVGTPQRETQRNFYAAAQNLQANPQQATSGALFTQTPSGQSSATYSQFN QQSLNSTAPPPPPPPPPSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGSTSQQSVPGHHVTP GHFLPSQNPTIHHQPAAAAVVPPPPPPPPAPGPHLIQQPSSHQQHSVAHGVGPVHAVTPGSHIHSQTAGH HLPPPPPPPGPAPHHHPPPHPTTGLQSLQAQHQHVVNSAPPPPPPPPPPPPASVLVSGHHSASGQALHHP PHQGPPLFPASAHPAVPPYPSQATHHTTLGPGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFC PHPHPGSVALPHGVQGPQQASPVPAQIPIHRAQVPPTFQNNYHGSGWH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_026984 |
ORF Size | 5604 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_026984.1, NP_081260.1 |
RefSeq Size | 7229 bp |
RefSeq ORF | 5607 bp |
Locus ID | 69188 |
UniProt ID | Q3UG20 |
MW | 204.5 kDa |
Gene Summary | Associates with chromatin regions downstream of transcriptional start sites of active genes and thus regulates gene transcription (By similarity). Chromatin interaction is mediated via the binding to tri-methylated histone H3 at 'Lys-4' (H3K4me3) (By similarity). Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation (PubMed:18854576, PubMed:18952892, PubMed:18818388). Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages including G1/S transition, S phase progression and mitotic entry (PubMed:19264965). Recruited to E2F1 responsive promoters by HCFC1 where it stimulates tri-methylation of histone H3 at 'Lys-4' and transcriptional activation and thereby facilitates G1 to S phase transition (By similarity). During myoblast differentiation, required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells (PubMed:19264965).[UniProtKB/Swiss-Prot Function] |
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