Kmt2e (NM_026984) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1810033J14Rik; 9530077A04Rik; D230038D11Rik; Mll5; NKp44L
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR223799 representing NM_026984, codon optimized. Due to the complexity of NM_026984, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATAGCGATCCCATTGGGGGTTGATACAACAGAAACATCCTACTTGGAAATGGCTGCAGGCTCAG AACCGGAATCCGTAGAAGCTAGCCCTGTGGTAGTTGAGAAATCCAACAGTTTTCCCCACCAGTTATACAC CAGCAGCTCCCACCACTCACACAGCTACATCGGTCTGCCCTATGCGGACCATAATTATGGTGCTCGTCCT CCTCCAACACCTCCAGCATCCCCTCCTCCATCAGGTCTCATTAGCAAAAACGAAGTAGGCATATTTACCA CTCCTAATTTTGATGAAACTTCCAGTGCTACTACAATCAGCACATCTGAAGATGGAAGTTACGGTACTGA TGTAACCAGGTGCATATGTGGTTTTACACATGATGACGGATACATGATCTGTTGTGACAAATGCAGTGTT TGGCAACATATTGACTGCATGGGGATTGACAGGCAGCATATTCCTGATACATACCTATGTGAACGTTGTC AGCCCAGGAGTTTGGATAAAGAGAGGGCAGTGCTACTGCAACGCCGGAAAAGAGAAAATATGTCAGATGG TGATACCAGTGCAACTGAAAGTGGTGATGAAGTTCCTGTGGAATTATATACTGCATTTCAGCATACTCCA ACTTCAATCACTTTAACTGCTTCCAGAGTTCCCAAGGTTACTGATAAAAGAAGGAAAAAAAGTGGGGAGA AAGAACAAAACTTTTCAAAATGTAAAAAAGCCTTTCGTGAAGGATCTAGGAAATCATCAAGGGTTAAGGG TTCAGCACCAGAAATTGATCCTTCATCTGATAGTTCAAATTTTGTTTGGGAAACAAAAATCAAAGCATGG ATGGATCGTTACGAAGAAGCAAATAATAACCAATATAGTGAAGGTGTTCAGAGAGAGGCACAAAGACTAG CTCAAAGACTCGGTAGTGGGAATGACAGCAAAGACATGAATAAATCAGAATTGAGTACCAACAACTCACT CTTCAGACCTCCTGTAGAGAGCCATATACAGAAGAATAAAAAAATTCTGAAATCTGCCAAAGACTTGCCT CCTGATGCACTTATCATTGAATATAGAGGGAAGTTTATGCTGAGAGAACAGTTTGAAGCAAATGGATATT TCTTTAAAAGGCCATACCCTTTTGTTTTATTCTACTCTAAGTTCCATGGGCTTGAAATGTGTGTTGATGC GAGGACTTTTGGGAATGAGGCTCGATTCATCAGGCGTTCTTGTACACCGAATGCAGAGGTGAGGCATGAA ATTGAAGAGGGGACCATACATCTCTACATTTATTCTATACAGAGTATTCCAAAAGGGACTGAAATTACCA TTGCCTTTGATTTTGACTACGGGAATTGCAAATACAAGGTGGACTGTGCATGCCTCAAGGAAAATCCAGA GTGCCCTGTTCTCAAGCGAAGCTCGGAATCCACAGAAAACATCAATAGTGGTTATGAGACCAGAAGGAAA AAAGGAAAAAAAGAGAAAGATACTTCAAAAGAAAAAGACATACAAAATCAGAATATGACTTTGGACTGTG AAGGAACAAACAACAAAATAAGGAGTCCAGAAACAAAACAGAGAAAGCTTTCTCCATTGAGGCTGTCGGT GTCAAACAACCAGGAACCAGATTTTATTGATGATATGGAAGAAAAAACTCCTATTAGCAATGAAGTGGAA ATGGAATCTGAGGAACAGATTGCAGAAAGGAAAAGGAAGATGACAAGAGAAGAAAGAAAAATGGAAGCAA TTTTGCAGGCTTTTGCCAGACTTGAGAAGAGAGAGAAAAGACGAGAACAAGCTTTGGAAAGGATCAGCAC AGCTAAGACAGAAGTTAAGCCTGAATGCAAAGAGTCGCAGGTCATCGCCGATGCCGAAGTGGTGCAGGAA CAAGTAAAAGAAGAAACTGCTATCAAGCCAGCCGCTGCCAAAGTGAACAGAACTAAGCAGAGAAAGAGCT TTTCTCGTAGTAGGACTCACATTGGACAGCAGCGTCGGAGACACAGAACTGTCAGCATGTGTTCAGACAT ACCGCCATCTTCTCCTGACATCGAAGTTCTGTCACAACAGAACGAGATTGAAAATACTGTACTTGCTATA GAACCAGAAACTGAAACTGCAGTAGCAGAAATAATTCCTGAAGCGGAAGTTCCAGCACTTAATAAGTGTC CCACAAAGTACCCCAAAACAAAGAAGCACTTGGTCAATGAATGGTTAAGTGAGAAGAATGAGAAGACAGG AAAACCTTCAGACAGCCTTTCAGAAAGGCCTCTGCGCATAACTACAGATCCAGAGGTGTTAGCTACACAG CTCAATTCTTTGCCAGGTCTCACTTACAGCCCCCATGTATACTCTACTCCTAAGCATTATATTAGATTTA CATCACCATTCCTTTCGGAAAAAAAGAGAAGAAAAGAAACTACTGAAAACATTTCTGGCTCATGCAAAAA GCGGTGGCTGAAGCAAGCCCTGGAGGAAGAAAATTCAACAATTTTACATAGATATCATTCACCTTGTCAG GAAAGATCCAGGAGTCCTACAGTTAATGGTGAAAACAAAAGTCCACTTCTGCTAAGCGACAGCTGCTCCC TCCCAGATTTAACCACACCACTAAAAAAGCGAAGACTTTACCAATTGTTAGATACTGCTTACTCAGAAAG CTCCACTCCTACCCCTTCACCATATGCTACACCAACTCACACAGACATCACTCCTACGGACCCAGCATTT GCCACTCCCCCACGGATCAAATCAGATGATGAAACTTACAGAAATGGTTATAAACCCATATATTCACCAG TGACCCCTGTAACCCCTGGCACACCAGGAAACACCATGCACTTTGAGAATATTTCTTCCCCAGAAAGTTC TCCAGAAATAAAGAGATGCACTTACAATCAAGAGGGATATGACCGACCTTCAAACATGCTAACGTTGGGG CCTTTTAGAAATTCCAATTTAACTGAGCTGGGCCTGCAAGAAATAAAGACTATTGGTTATACCAGCCCTA GGAGCAGGACTGAGGTTAACAGGCCTTGCCCAGGGGAGAAGGAGTCTGTGTCAGACCTTCAACTGGGACT CGATGCAGTCGAGCCAGCTGCCTTACAGAAATCCATGGAAACACCAGCCCATGACAGGACTGAGCCCAGC AACCAACTGGATTCGACCCACTCTGGACGGGGCACAATGTATTCATCCTGGGTAAAGAGTCCTGACAGAA CAGGAGTTAACTTCTCAGTAAACTCCAACTTGAGGGACTTAACACCCTCTCATCAGTTGGAGACTGGAGG CGGCTTCCGAGTAAGTGAGTCCAAGTGCCTGATTCAGCAGGATGACACTAGAGGCATGTTTCTGGGAGCG GCTGTCTTTTGTACTTCTGAAGACGGGCTTGCCTCTGGTTTTGGACGGACTGTTAATGACAATTTGATCG ACGGGAGTTGCACGCCCCAGAATCCACCACAAAAGAAAAAGGTTTCTCTATTAGAATACCGTAAGAGACA ACGTGAAGCTAGGAAGAGTGGGTCTAAGCCAGAGAACTTTGCTTTGATTAGTGTGTCACCTCACCCGAGT GGGAGCCTGAGCAGCAGTGGCGACGGGTGTGTCCACAGCAGTGAAAACGGGGAGCAGGCAGAAAACCAGG CTAGCTTGCCTTTACCACCGCCTGCTGCCGCCGCCGCCGCCACCGCCGCCGCCGCTTACAGTGCCTCTTC TGAAGAAGGCAGCAGTAACTGTCCTGTTAAAGACGCCAATTCCAGTGAGAAGAAAGACCCAGAAGTTCAG TGGACTGCCTCCACTTCAGTGGAGCAAGTGAGAGAGAGAAGTTACCAGAGAGCCTTACTGCTCAGTGACC ACCGCAAAGACAAAGACAGTGGGGGAGAGTCACCATGTGTCTCATGCTCACCGAGTCATGTTCAGTCTCC ACCTTCATCTCATTCAAATCACATTCCCCAGGTGCACGCTCAGAGCCTAGCTCCTTCTTTGAGTGAACTC ATGGCAGACCCTGATGCGGAAGGTACAGAAGCCACAAGTACAAGTGAATGTCCATCCCCTGACACTTCTC AGAGCCCTTCTAAAACAAGCAAGCCTGGTTCACCAGGGCCAATCAATCCTGCTCAGTCACATGGGAAAAT ACTCACAAAACCAGATAGCCACTGGGAGGCAACTGCCACTGTATCAGAAGCTGACAACAGTGTTCACCAA AACCCAGAGCCTCAGCACAGACAGCTGTCAAGTAACACCCCAGCACTTTCTCAGAACCATGCTCCTCAGG CCCACGCTCTGAGTGCGAATGATCAACTTCCGCAAAAGCTGCCTTCTGCACCGACGAAGTTGCACTGTCC TCCGTCACCTCACACAGAAAACCCTCCCAAGTCCTCCACACCTCACACCCCCGTGCAGCATGGTTACCTC TCGCCAAAGCCTCCTTCACAGCACTTAGGATCTCCCTTCAGGCCTCACCATTCACAATCACCTCAAGTTG GAACTCCTCAACGAGAGACTCAGCGCAACTTTTACGCCGCAGCCCAGAATCTGCAGGCCAACCCCCAGCA GGCTACCTCAGGAGCTCTTTTTACTCAAACTCCTTCAGGGCAGTCATCCGCAACTTATTCCCAGTTCAAT CAGCAAAGCCTCAATAGTACGGCCCCGCCTCCACCTCCACCGCCGCCTCCTAGTAGTTATTATCAAAACC AGCAGCCAAGTGCTAATTTTCAGAACTATAACCAACTGAAGGGAAGCCTTAGCCAGCAGACGGTCTTCAC AAGCGGGCCTAATCAAGCCCTGCCTGGCTCTACTTCACAGCAGTCCGTGCCTGGGCACCACGTCACACCT GGGCACTTCCTGCCATCCCAGAACCCCACAATACACCATCAACCTGCCGCGGCCGCCGTCGTGCCACCTC CTCCTCCGCCCCCGCCCGCACCCGGCCCACATCTGATTCAGCAGCCAAGCAGTCATCAGCAGCACAGTGT AGCACATGGGGTGGGCCCAGTACATGCTGTGACACCCGGCTCCCACATTCATTCACAGACCGCCGGACAT CACCTGCCTCCCCCGCCTCCGCCCCCAGGCCCCGCACCACATCACCACCCACCCCCCCATCCGACGACCG GTCTGCAGAGCCTGCAAGCCCAGCATCAGCACGTCGTTAACTCCGCTCCACCTCCTCCACCTCCACCACC CCCACCCCCGCCCGCTAGCGTGTTGGTCAGCGGGCATCACTCCGCCAGTGGTCAGGCCCTGCACCACCCT CCACACCAGGGTCCTCCCCTCTTCCCTGCAAGCGCTCACCCAGCCGTCCCTCCATATCCAAGCCAGGCGA CCCACCACACCACCCTGGGCCCCGGCCCTCAACACCAGCCATCTGGCACTGGCCCTCACTGTCCTCTCCC CGTCGCAGGGCCACACTTGCAGCCTCAGGGGCCAAATAGCATACCTACTCCTACAGCTTCAGGTTTTTGT CCTCACCCCCACCCAGGTAGCGTGGCGCTGCCACACGGCGTGCAGGGTCCTCAGCAGGCCTCCCCTGTGC CCGCGCAGATTCCAATCCACAGGGCCCAAGTGCCTCCCACTTTCCAGAATAACTATCACGGTTCCGGATG GCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR223799 representing NM_026984 Red=Cloning site Green=Tags(s) MSIAIPLGVDTTETSYLEMAAGSEPESVEASPVVVEKSNSFPHQLYTSSSHHSHSYIGLPYADHNYGARP PPTPPASPPPSGLISKNEVGIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSV WQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTP TSITLTASRVPKVTDKRRKKSGEKEQNFSKCKKAFREGSRKSSRVKGSAPEIDPSSDSSNFVWETKIKAW MDRYEEANNNQYSEGVQREAQRLAQRLGSGNDSKDMNKSELSTNNSLFRPPVESHIQKNKKILKSAKDLP PDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHE IEEGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENPECPVLKRSSESTENINSGYETRRK KGKKEKDTSKEKDIQNQNMTLDCEGTNNKIRSPETKQRKLSPLRLSVSNNQEPDFIDDMEEKTPISNEVE MESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKPECKESQVIADAEVVQE QVKEETAIKPAAAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIPPSSPDIEVLSQQNEIENTVLAI EPETETAVAEIIPEAEVPALNKCPTKYPKTKKHLVNEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQ LNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKKRRKETTENISGSCKKRWLKQALEEENSTILHRYHSPCQ ERSRSPTVNGENKSPLLLSDSCSLPDLTTPLKKRRLYQLLDTAYSESSTPTPSPYATPTHTDITPTDPAF ATPPRIKSDDETYRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRCTYNQEGYDRPSNMLTLG PFRNSNLTELGLQEIKTIGYTSPRSRTEVNRPCPGEKESVSDLQLGLDAVEPAALQKSMETPAHDRTEPS NQLDSTHSGRGTMYSSWVKSPDRTGVNFSVNSNLRDLTPSHQLETGGGFRVSESKCLIQQDDTRGMFLGA AVFCTSEDGLASGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKPENFALISVSPHPS GSLSSSGDGCVHSSENGEQAENQASLPLPPPAAAAAATAAAAYSASSEEGSSNCPVKDANSSEKKDPEVQ WTASTSVEQVRERSYQRALLLSDHRKDKDSGGESPCVSCSPSHVQSPPSSHSNHIPQVHAQSLAPSLSEL MADPDAEGTEATSTSECPSPDTSQSPSKTSKPGSPGPINPAQSHGKILTKPDSHWEATATVSEADNSVHQ NPEPQHRQLSSNTPALSQNHAPQAHALSANDQLPQKLPSAPTKLHCPPSPHTENPPKSSTPHTPVQHGYL SPKPPSQHLGSPFRPHHSQSPQVGTPQRETQRNFYAAAQNLQANPQQATSGALFTQTPSGQSSATYSQFN QQSLNSTAPPPPPPPPPSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGSTSQQSVPGHHVTP GHFLPSQNPTIHHQPAAAAVVPPPPPPPPAPGPHLIQQPSSHQQHSVAHGVGPVHAVTPGSHIHSQTAGH HLPPPPPPPGPAPHHHPPPHPTTGLQSLQAQHQHVVNSAPPPPPPPPPPPPASVLVSGHHSASGQALHHP PHQGPPLFPASAHPAVPPYPSQATHHTTLGPGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFC PHPHPGSVALPHGVQGPQQASPVPAQIPIHRAQVPPTFQNNYHGSGWH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_026984
ORF Size	5604 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_026984.1, NP_081260.1
RefSeq Size	7229 bp
RefSeq ORF	5607 bp
Locus ID	69188
UniProt ID	Q3UG20
MW	204.5 kDa
Gene Summary	Associates with chromatin regions downstream of transcriptional start sites of active genes and thus regulates gene transcription (By similarity). Chromatin interaction is mediated via the binding to tri-methylated histone H3 at 'Lys-4' (H3K4me3) (By similarity). Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation (PubMed:18854576, PubMed:18952892, PubMed:18818388). Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages including G1/S transition, S phase progression and mitotic entry (PubMed:19264965). Recruited to E2F1 responsive promoters by HCFC1 where it stimulates tri-methylation of histone H3 at 'Lys-4' and transcriptional activation and thereby facilitates G1 to S phase transition (By similarity). During myoblast differentiation, required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells (PubMed:19264965).[UniProtKB/Swiss-Prot Function]

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