Zc3h13 (NM_026083) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2600010B19Rik; 3110050K21Rik; 4930570G11Rik; C87618
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR223805 representing NM_026083, codon optimized. Due to the complexity of NM_026083, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAAGATTAGGCGAAAAGTCACTGTGGAGAATACAAAAACCATCTCTGAAAGCACCAGCAGGAGGC CAAGTGTGTTTGAACGCCTCGGCCCATCCACAGGCTCTACTACCGAGACTCAGTGTAGGAACTGGCTTAA AACTGGGTCCTGTTTGTACGGGAACACCTGCCGGTTCATCCATGGTCCTAGCCCACGGGGTAAAGGCTAC AGTTCTAACTATAGAAGATCTCCTGAGAGGCCCACCGGCGACCTCCGCGAAAGAATGAAGAACAAGCGAC AGGACGTGGACAGTGAGTCACAGAAGCGGAATACCGAAGAACCAAGCAGCCCCGTCAGAAAGGAATCAAG CAGGGGCCGGCACCGGGATAAGGAAGATATCAAAATCGTGAAAGAGCGCACCCCCGAGTCTGAAGAAGAG AATGTGGAATGGGAGACGAATCGCGACGATAGCGATAACGGTGATATCAACTACGACTACGTTCACGAAC TTAGTCTTGAAATGAAGCGACAGAAGATTCAGAGGGAGCTGATGAAATTGGAACAGGAAAATATGGATAA GAGGGAAGAAATAATTATTCAGAAGGAGGTCAGCCCCGAAGTGGTGCGCAGCAAACTGAGCCCCTCTCCT TCACTGAGAAAGAGCTCCAAATCCCCAAAGCGCAAATCTTCACCCAAAGCCTCCTCTGCTGGGAAGAAAG AACGAAAAGCGGCCGTCGTGGCCTCCCCCCTCCTGGATCAGCAGAGGAATAGTAAAGGGAACCAGTCTAA GAAAAAGGGGCCTCGGACACCTAGCCCCCCACCCCCCATCCTGGAAGACATCATCCTTGGTAAAAAGTAT AAGGAGAAGTACAAAGTCAAAGACCGAATCGAAGAAAAACCCCGCGATGGAAAGGATCGCGGAAGAGACT TTGAGAAGCAGAGAGAGAAACGGGATAAACCTCGCTCAAGTTCTCCTGGGCAGCACCACAGCCCACTTTC AAGTAGACATCACAGCTCATCTTCCCAGAGCGGCAGCTCTATCCAGCGGCACAGTCCCAGTCCCCGGAGG AAAAGGACACCATCCCCGTCCTATCAGAGAACCCTGACTCCTAGCCTGAGGCGGTCCGCTAGTCCATACC CTACTCACTGTCTCTCCAGCCCTCAGCGAAAGCAGAGCCCTCCTCGACACCGCAGCCCTATGAGAGAAAA GGGGCGACATGACCATGAGCGCACATCCCAGAGCCATGACAGGAGACACGAAAGGAGAGAGGAGACACGC GGCAAGCGAGATCGGGAGAAGGACACCCGCGAGGAGCGCGAGTCCGAGCACGACCACCGGGACGATAGGG AGCCCAGGGACAGTCGCGACAGGAGGGATACTAGAGACAGACGAGAGCTGCGAGACTCCCGGGACATGAG AGATTCTCGAGAGATGCGGGACTACTCTAGAGACGCCAAAGAGAGCCGAGATCCAAGAGACAGTCGATCT GCTCGAGATGTCCATGATTACCGGGACCGAGAGGCCCGCGATGCTCACGCTAGGGACGTGAGAGACGCGC GCGATGCTAGAGATGCCCGGGACGCAAGAGATATCAGAGATGTACGGGATGTCCGGGACGTCCGGGACGT GAGAGACGTTAGAGATGTTCGCGACGTGAGGGACGTACGAGACGTCAGAGACGCAAGAGACGTGCGCGAC GTGCGGGACGCTCGAGACGTGCGCGACGTGCGCGATGTAAGAGACGGACACAGGAAGGAGGACGTCTATC AGGAAGAAGCCAGATCCTACGGGCGAAATCACCTGAGAGAAGAGTCAAGCCGGGTTGAGCTGAGGAACGA CTCTCGCAATGAGAGTCGGAGCGAGATTCGCAACGATAGGATGGGCAGGTCACGAGGTCGGGGCCCTGAA CTCCCTGAGAAGGGGTCCCGAGGTACACGAGGAAGCCAGATGGACTCTCATTCATCTGGGTCTAACTACC ACGATTCCTGGGAGACAAGATCCTCATACCCCGAACGGGACAGGTATCCTGAACGCGACACGAGGGACCC AGCACGCGACAGTAGTTTCGAACGAAGGCACGGGGAGCGCGACAGGAGGGATAACAGAGAGCGGGACCAG AGGCCGTCCTCACCCATTCGCCACCAGGGAAGATCTGAGGAGCTCGAGAGGGACGAGCGGAGAGAAGAGC GGAGGATCGACAGAGTGGATGAACGACGAGACGACCGGGTGAGGGATCGCGACCGGGATCGGGAGAGAGA GCGGGAGCGAGAAAGGGAAAGGGAACGCGAAAGAGAAAAGGAGCGGGAGCGCGAGCTGGAGAGAGAGAGA GCCCGCGAGAGAGAGCGGGAACGGGAAAAGGAACGGGAGCGCGAAAGGGAGCGCGAACGCGATCAGAGAG ATCGAGATCATGATAGGGAGCGGGAGCGGGAGCGGGAGAGGGAACGGGAAAAGGAGCGGGAACGGGAAAG GGAGGAGCGAGAGAGAGAAAGGGAACGGGAGAGAGAGAGGGAACGCGAGAGAGAGCGAGAGAGAGAGAGG GAACGGGAGCGAGAGCGCGAAAGGGAACGCGCTCGAGAACGCGAAAAGGAACGAGAAAGACAGAGAGAGT GGGAGGACAAAGATAAAGGGAGAGACGACCGGCGGGAAAAACGCGAAGACATCCACGTGCGGGAGGACCG GATTCCCAGAGACTCTCACGAAGAGCGGAAGAGTAAAAAGCGGTATAGAAACGAGGGCAGTCCGTCACCC CGGCAGTCTCCAAAAAGGAGACGGGAGCATTCCCCCGATTCCGATACCTATCACTCAGGCGATGATAAGA ACGAAAAGCACCGGCTGTTGTCCCAAGTCGTCAGGCCACAGGAAAGCCGCAGCCTCTCCCCCTCTCACCT CACTGAAGACCGACAAGGACGGTGGAAGGAAGAGGACCGAAAATCCGAACGGAAGGAATCCTCTCGAAGG TATGAGGAGCAAGAACTGAAGGAGAAATTGTCATGTGGAGACAGACAGAGAGAGCAAGCCGAATCAGTCG ACAGCAGCCGCGTGCGCGCACAGGACCTGTTGTCCCATAGGCAGGCTGAGGACAGAGATAGAGATGGCTC CGACCGCGCTCACGATGAGAAGAAAAAAGCCAAGGCCCCCAAGAAGCCCGTGAAGAAAAAAAAAGAAGAG GACGTGGGCGTGGAGCGAGGAAACCTGGAAACACATGAAGATTCCCAGGTCTTCAGCCCTAAGAAAGGTC AGAAGAAGAAGAACATAGAGAAGAAACGAAAGCGCTCCAAGGGCGACAGTGATGTCAGCGACGAGGAGGC CGCCCCTCAAAACAAGAAGAAGCGCGGACCCCGCACCCCCCCTCTGGCAATAAAGGAGGAGTTGGCCGAC ATCAGCACCGACAAGGACGGCGTACTGGAGGACCCATTGAAAAAAGAAAACACCGCTTTTAGCGACTGGT CTGATGAAGATGTGCCTGATAGGACAGAGGGCCTGGAGGCCGAGCACACAGCAGCAACAGCCACCCCTGG TTCCACTCCGAGTCCTTTGTCTAGCTTGCTCCCTCCTCCACCACCCGTGGCCGCTGCTTCCACCGCTGCC ACTGCCCTTGCCTCCAGTGCCGTGAGCGCCACAACGTCCGCTACATCCAGCTCAAGCGCAGCAACAAGTA ACACCAACGGGTCAGAGGACAGCCATCGCAAGTGTCACAGGGCCCGGGGGGAGAAGGTCGAGGTATCCCA CGTGACACTGGAGGATACTCCGCACAGGAAGCTGGTGGATCAGAAACGATCTTCCAGCCTGGGCTCAAAC CGCTCCCACAGATCCCACACCTCCGGAAGACTGAGGTCCCCATCCAATGACAGTGCGCACAGATCAGGGG ATGATCAGGGTAGCCGCAAGAGGGTACTGCACTCAGGATCTCGCGACCGAGAGAAAACCAAAAGCCTGGA GATAACAGGCGAAAGAAAGAGTAGGATTGATCAGCTGAAAAGAGGGGAGCCTAGCCGGAGCACGAGTAGT GACCGGCAGGATAGCCGAAGTCATAGCTCTAGACGCTCCTCCCCTGAGTCAGACCGCCAGGTCCATAGCC GGTCAGGATCCTTTGACAGCCGAGATCGGTTGCAGGAAAGGGACCGGTACGAACATGATAGAGAACGCGA GCGCGACCGGAGGGACCCGAGACAGCGGGAGTGGGACCGCGAGGCCGAGAAAGAATGGCCCCGCACACGG GATAGGGACCGGCTGCGAGAGAGGGACCGCGACCGGGACAGACGAAGGGACCTGGACCGAGAACGGGAGC GGCTCATTTCAGACCCTATGGAACGCGACAGAGAACGGGAGAGGACCTTTGAGACTTCTCAGCTTGAGAG TGGCAAAAGATCCGAAGTCAAACTCGAGAGCGAGCACGAGAGGGACCTGGAAGGAAGTTCAAGAGATTCA GTAGCCTTGGATAAAGAAAGAATGGATCGAGACTTGGGCTCAGTTCAGGGTTTTGAAGATGTGTCCAAGG CAGAGCGCACCGAATCATTGGAGGGGGACGACGAATCCAAGCTGGACGACGCTCACTCTCTGGGCAGTGG AGCAGGAGAAGGCTACGAACCCATCTCAGATGACGAGCTGGACGAAATACTGGCCGGCGATGCTGAGAAG CGAGAGGACCAGCAGGAAGAAGAGAAGATGCCCGACCCTTTGGATGTGATAGATGTGGATTGGTCTGGAC TCATGCCTAAGCACCCTAAGGAGCCTAGGGAGCCCGGTGCAGCCCTGCTGAAGTTTACACCCGGAGCCGT GCTTCTGCGAGTGGGTATAAGTAAAAAATTGGCTGGCTCCGAATTGTTCACCAAGGTCAAGGAGACTTGT CAACAGCTTGTGGAAAAGCCCAAGGACGCCGATTCCCTCTTCGAGCACGAACTGGGTGCCCTCAATATGG CCGCCCTTCTGCGCAAGGAAGAGCGAGCATCCCTTCTGAGCGACCTTGGGCCTTGTTGCAAAGCTCTGTG CTTTAGACGGGACAGCGCCATTAGAAAGCAGCTCGTCAAAAACGAAAAGGGTACCGTGAAACAGGCGTAC ACCAACACCCCTATGGTGGATAACGAACTCTTGCGCCTCAGCTTGCGCCTTTTTAAGAAAAAAGCTACTT GTCATGCCCCTGGTCAAGAGAAGACAGAAGATGGCAAGCTGGGACCTTGCTCCATACAACAGGAACTTTG CGTTAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR223805 representing NM_026083 Red=Cloning site Green=Tags(s) MSKIRRKVTVENTKTISESTSRRPSVFERLGPSTGSTTETQCRNWLKTGSCLYGNTCRFIHGPSPRGKGY SSNYRRSPERPTGDLRERMKNKRQDVDSESQKRNTEEPSSPVRKESSRGRHRDKEDIKIVKERTPESEEE NVEWETNRDDSDNGDINYDYVHELSLEMKRQKIQRELMKLEQENMDKREEIIIQKEVSPEVVRSKLSPSP SLRKSSKSPKRKSSPKASSAGKKERKAAVVASPLLDQQRNSKGNQSKKKGPRTPSPPPPILEDIILGKKY KEKYKVKDRIEEKPRDGKDRGRDFEKQREKRDKPRSSSPGQHHSPLSSRHHSSSSQSGSSIQRHSPSPRR KRTPSPSYQRTLTPSLRRSASPYPTHCLSSPQRKQSPPRHRSPMREKGRHDHERTSQSHDRRHERREETR GKRDREKDTREERESEHDHRDDREPRDSRDRRDTRDRRELRDSRDMRDSREMRDYSRDAKESRDPRDSRS ARDVHDYRDREARDAHARDVRDARDARDARDARDIRDVRDVRDVRDVRDVRDVRDVRDVRDVRDARDVRD VRDARDVRDVRDVRDGHRKEDVYQEEARSYGRNHLREESSRVELRNDSRNESRSEIRNDRMGRSRGRGPE LPEKGSRGTRGSQMDSHSSGSNYHDSWETRSSYPERDRYPERDTRDPARDSSFERRHGERDRRDNRERDQ RPSSPIRHQGRSEELERDERREERRIDRVDERRDDRVRDRDRDREREREREREREREREKERERELERER AREREREREKERERERERERDQRDRDHDREREREREREREKEREREREERERERERERERERERERERER ERERERERERAREREKERERQREWEDKDKGRDDRREKREDIHVREDRIPRDSHEERKSKKRYRNEGSPSP RQSPKRRREHSPDSDTYHSGDDKNEKHRLLSQVVRPQESRSLSPSHLTEDRQGRWKEEDRKSERKESSRR YEEQELKEKLSCGDRQREQAESVDSSRVRAQDLLSHRQAEDRDRDGSDRAHDEKKKAKAPKKPVKKKKEE DVGVERGNLETHEDSQVFSPKKGQKKKNIEKKRKRSKGDSDVSDEEAAPQNKKKRGPRTPPLAIKEELAD ISTDKDGVLEDPLKKENTAFSDWSDEDVPDRTEGLEAEHTAATATPGSTPSPLSSLLPPPPPVAAASTAA TALASSAVSATTSATSSSSAATSNTNGSEDSHRKCHRARGEKVEVSHVTLEDTPHRKLVDQKRSSSLGSN RSHRSHTSGRLRSPSNDSAHRSGDDQGSRKRVLHSGSRDREKTKSLEITGERKSRIDQLKRGEPSRSTSS DRQDSRSHSSRRSSPESDRQVHSRSGSFDSRDRLQERDRYEHDRERERDRRDPRQREWDREAEKEWPRTR DRDRLRERDRDRDRRRDLDRERERLISDPMERDRERERTFETSQLESGKRSEVKLESEHERDLEGSSRDS VALDKERMDRDLGSVQGFEDVSKAERTESLEGDDESKLDDAHSLGSGAGEGYEPISDDELDEILAGDAEK REDQQEEEKMPDPLDVIDVDWSGLMPKHPKEPREPGAALLKFTPGAVLLRVGISKKLAGSELFTKVKETC QQLVEKPKDADSLFEHELGALNMAALLRKEERASLLSDLGPCCKALCFRRDSAIRKQLVKNEKGTVKQAY TNTPMVDNELLRLSLRLFKKKATCHAPGQEKTEDGKLGPCSIQQELCVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_026083
ORF Size	5187 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_026083.2, NP_080359.2
RefSeq Size	6151 bp
RefSeq ORF	5190 bp
Locus ID	67302
UniProt ID	E9Q784
MW	203.8 kDa
Gene Summary	Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29535189, PubMed:29547716). Acts as a key regulator of m6A methylation by promoting m6A methylation of mRNAs at the 3' UTR (PubMed:29547716). Controls embryonic stem cells (ESCs) pluripotency via its role in m6A methylation (PubMed:29547716). In the WMM complex, anchors component of the MACOM subcomplex in the nucleus (PubMed:29547716). Also required for bridging WTAP to the RNA-binding component RBM15 (RBM15 or RBM15B) (PubMed:29535189).[UniProtKB/Swiss-Prot Function]

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