Myt1l (NM_001093776) Mouse Tagged ORF Clone
CAT#: MR223831
- TrueORF®
Myt1l (Myc-DDK-tagged) - Mouse myelin transcription factor 1-like (Myt1l), transcript variant 3
ORF Plasmid: tGFP
"NM_001093776" in other vectors (2)
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CNY 11,780.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2900046C06Rik; 2900093J19Rik; C630034G21Rik; mKIAA1106; Nztf1; Pmng1; Png-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR223831 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTGGACTCTGAGGAGAAGCGCCATCGCACACGGTCCAAAGGGGTTCGAGTTCCTGTGGAGCCAG CCATACAAGAGCTGTTCAGCTGTCCCACTCCAGGCTGCGACGGCAGTGGTCACGTCAGTGGCAAATATGC ACGACACAGAAGTGTATATGGTTGTCCCTTGGCTAAAAAAAGAAAAACGCAAGATAAACAGCCCCAAGAA CCTGCTCCCAAGCGAAAACCATTTGCAGTAAAAGCAGATAGTTCCTCAGTAGACGAATGTTATGAGAGTG ATGGTACTGAAGACATGGATGATAAGGAGGAAGATGATGATGAGGAGTTCTCTGAAGACAATGATGAGCA AGGGGATGATGACGACGAAGATGAGGTGGATCGGGAAGACGAGGAGGAGATCGAGGAGGAAGATGATGAA GAAGATGATGATGATGAAGATGGTGACGATGTAGAAGAGGAAGAAGAGGATGATGATGAAGAGGAGGAAG AAGAGGAAGAGGAAGAAGAAAATGAAGACCATCAAATGAGTTGTACTCGAATAATGCAGGACACAGACAA GGATGATAACAACAATGATGAGTATGATAACTATGATGAACTGGTAGCTAAGTCGCTATTAAATCTTGGC AAAATTGCTGAGGATGCAGCATACCGAGCCAGGACTGAATCAGAGATGAACAGCAATACCTCCAATAGTC TGGAGGACGATAGTGACAAAAACGAAAACCTCGGTCGGAAAAGCGAACTGAGTCTAGACTTAGACAGTGA TGTTGTTAGAGAAACAGTGGACTCCCTTAAGCTGTTAGCACAAGGACATGGTGTTGTGCTATCAGAGAAT ATCAGTGACAGAAGTTATGCTGAGGGGATGTCACAGCAGGACAGTAGAAATATGAACTATGTCATGCTAG GGAAGCCCATGAACAATGGACTCATGGAGAAGATGGTGGAGGAGAGTGATGAGGAAGTGTGTCTAAGTAG TCTAGAGTGCCTGAGGAACCAGTGCTTTGACCTGGCCAGGAAACTCAGCGAGACCAACCCACAGGACAGG AGTCAGCCACCCAACATGAGTGTGCGCCAACATGTCCGGCAAGAGGACGACTTCCCTGGGAGGACGCCAG ACAGGAGCTACTCGGATATGATGAACCTTATGCGGCTGGAGGAGCAGCTCAGTCCCAGGTCTAGAACGTT CTCCAGCTGTGCCAAGGAGGATGGGTGTCATGAGAGGGATGATGACACCACCTCAGTGAACTCAGACAGG TCTGAGGAAGTGTTTGACATGACCAAGGGCAACCTGACTCTGCTAGAGAAAGCCATTGCCTTGGAGACAG AGAGAGCCAAGGCCATGCGGGAGAAGATGGCCATGGATGCTGGGAGAAGGGATAACCTGAGATCCTATGA GGACCAGTCTCCAAGACAGCTGGCTGGGGAAGACAGAAAATCCAAATCCAGTGACAGCCATGTCAAAAAG CCATACTATGATCCCTCAAGAACAGAAAAGAGAGAGAGCAAGTGTCCAACCCCCGGGTGTGATGGAACCG GCCACGTAACTGGGCTTTACCCGCATCACCGCAGTCTGTCTGGATGCCCGCACAAAGATAGGGTCCCTCC AGAAATTCTTGCCATGCATGAAAATGTTCTCAAGTGTCCCACTCCAGGCTGCACAGGGCGAGGGCATGTG AATAGCAACAGGAACTCGCACAGAAGCCTCTCTGGATGCCCCATTGCTGCTGCAGAAAAACTGGCAAAGG CCCAAGAGAAACACCAGAGCTGTGATGTGTCCAAATCCAACCAGGCCTCAGACCGAGTCCTCAGGCCAAT GTGCTTTGTCAAACAGCTTGAGATTCCTCAGTATGGCTACAGAAACAATGTTCCCACAACCACACCACGC TCCAACCTGGCCAAGGAGCTTGAGAAATACTCCAAGACTTCGTTTGAGTACAACAGTTACGACAACCATA CTTATGGCAAAAGAGCCATAGCTCCCAAGGTGCAAACCAGGGACATATCCCCCAAAGGATATGACGATGC CAAGCGGTACTGCAAGAATGCCAGCCCCAGCAGCAGCACCACCAGCAGCTATGCACCTAGCAGCAGCAGC AACCTCAGCTGTGGTGGTGGCAGCAGCGCCAGTAGCACGTGTAGCAAGAGCAGCTTTGACTACACACATG ACATGGAGGCCGCACACATGGCAGCCACAGCCATTCTCAACCTGTCCACACGTTGTCGTGAAATGCCACA GAACCTGTCCACCAAGCCACAGGACCTGTGTACTGCCCGGAACCCAGACATGGAGGTGGATGAGAATGGC ACCCTGGACCTGAGCATGAACAAGCAGAGGCCTCGAGACAGCTGCTGCCCAGTCCTGACACCCCTGGAAC CCATGTCTCCGCAGCAGCAGGCCGTGATGAGCAGCCGATGCTTCCAGCTGAGCGAGGGGGATTGCTGGGA CTTGCCTGTAGACTACACCAAAATGAAGCCTCGGAGGGTAGATGAGGATGAGCCCAAAGAGATTACCCCA GAAGACTTGGACCCATTCCAGGAGGCTCTGGAAGAAAGACGGTATCCAGGGGAGGTGACCATCCCAAGCC CCAAACCCAAGTACCCTCAGTGCAAGGAAAGCAAAAAGGACTTAATAACTCTGTCTGGCTGCCCCCTGGC GGACAAAAGCATTCGAAGTATGCTGGCCACCAGTTCCCAAGAGCTCAAGTGCCCCACCCCTGGCTGTGAC GGTTCTGGACACATCACTGGCAATTACGCTTCTCATCGAAGCCTTTCTGGGTGCCCGAGAGCAAAGAAGA GTGGCATCCGGATAGCACAGAGCAAAGAGGACAAGGAAGACCAGGAGCCAATCAGGTGTCCGGTACCTGG CTGTGACGGTCAGGGACACATCACTGGGAAGTATGCATCCCACCGCAGCGCCTCCGGGTGTCCCTTGGCA GCCAAGAGGCAGAAAGATGGGTACCTTAATGGCTCCCAGTTCTCCTGGAAGTCGGTCAAGACGGAGGGCA TGTCCTGCCCTACCCCCGGGTGTGATGGGTCAGGACACGTCAGTGGCAGCTTCCTCACACACCGCAGCTT GTCAGGATGTCCAAGAGCCACATCAGCAATGAAGAAAGCAAAGCTGTCTGGAGAACAGATGTTGACTATC AAGCAGCGAGCCAGCAACGGTATAGAAAATGATGAAGAAATCAAGCAGTTAGATGAAGAGATCAAGGAGC TTAATGAGTCCAATTCCCAGATGGAGGCTGACATGATCAAACTCAGAACTCAGATCACCACAATGGAGAG CAACCTGAAGACGATTGAGGAGGAGAACAAAGTCATTGAACAGCAGAATGAGTCGCTCTTGCACGAGTTG GCCAACCTGAGCCAGTCCCTGATCCACAGCCTCGCCAACATCCAGCTGCCTCACATGGATCCAATCAATG AACAAAATTTTGATGCTTACGTGACTACTTTGACGGAAATGTATACAAATCAAGATCGTTATCAGAGTCC AGAAAATAAAGCCCTACTGGAAAATATAAAGCAGGCTGTGAGAGGAATTCAGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223831 protein sequence
Red=Cloning site Green=Tags(s) MDVDSEEKRHRTRSKGVRVPVEPAIQELFSCPTPGCDGSGHVSGKYARHRSVYGCPLAKKRKTQDKQPQE PAPKRKPFAVKADSSSVDECYESDGTEDMDDKEEDDDEEFSEDNDEQGDDDDEDEVDREDEEEIEEEDDE EDDDDEDGDDVEEEEEDDDEEEEEEEEEEENEDHQMSCTRIMQDTDKDDNNNDEYDNYDELVAKSLLNLG KIAEDAAYRARTESEMNSNTSNSLEDDSDKNENLGRKSELSLDLDSDVVRETVDSLKLLAQGHGVVLSEN ISDRSYAEGMSQQDSRNMNYVMLGKPMNNGLMEKMVEESDEEVCLSSLECLRNQCFDLARKLSETNPQDR SQPPNMSVRQHVRQEDDFPGRTPDRSYSDMMNLMRLEEQLSPRSRTFSSCAKEDGCHERDDDTTSVNSDR SEEVFDMTKGNLTLLEKAIALETERAKAMREKMAMDAGRRDNLRSYEDQSPRQLAGEDRKSKSSDSHVKK PYYDPSRTEKRESKCPTPGCDGTGHVTGLYPHHRSLSGCPHKDRVPPEILAMHENVLKCPTPGCTGRGHV NSNRNSHRSLSGCPIAAAEKLAKAQEKHQSCDVSKSNQASDRVLRPMCFVKQLEIPQYGYRNNVPTTTPR SNLAKELEKYSKTSFEYNSYDNHTYGKRAIAPKVQTRDISPKGYDDAKRYCKNASPSSSTTSSYAPSSSS NLSCGGGSSASSTCSKSSFDYTHDMEAAHMAATAILNLSTRCREMPQNLSTKPQDLCTARNPDMEVDENG TLDLSMNKQRPRDSCCPVLTPLEPMSPQQQAVMSSRCFQLSEGDCWDLPVDYTKMKPRRVDEDEPKEITP EDLDPFQEALEERRYPGEVTIPSPKPKYPQCKESKKDLITLSGCPLADKSIRSMLATSSQELKCPTPGCD GSGHITGNYASHRSLSGCPRAKKSGIRIAQSKEDKEDQEPIRCPVPGCDGQGHITGKYASHRSASGCPLA AKRQKDGYLNGSQFSWKSVKTEGMSCPTPGCDGSGHVSGSFLTHRSLSGCPRATSAMKKAKLSGEQMLTI KQRASNGIENDEEIKQLDEEIKELNESNSQMEADMIKLRTQITTMESNLKTIEEENKVIEQQNESLLHEL ANLSQSLIHSLANIQLPHMDPINEQNFDAYVTTLTEMYTNQDRYQSPENKALLENIKQAVRGIQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001093776 |
ORF Size | 3555 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001093776.1, NP_001087245.1 |
RefSeq Size | 7104 bp |
RefSeq ORF | 3558 bp |
Locus ID | 17933 |
MW | 132.8 kDa |
Gene Summary | Transcription factor that plays a key role in neuronal differentiation by specifically repressing expression of non-neuronal genes during neuron differentiation (PubMed:28379941). In contrast to other transcription repressors that inhibit specific lineages, mediates repression of multiple differentiation programs (PubMed:28379941). Also represses expression of negative regulators of neurogenesis, such as members of the Notch signaling pathway, including HES1 (PubMed:28379941). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (PubMed:20107439, PubMed:24243019, PubMed:27281220). Directly binds the 5'-AAGTT-3' core motif present on the promoter of target genes and represses transcription by recruiting a multiprotein complex containing SIN3B (PubMed:28379941). The 5'-AAGTT-3' core motif is absent from the promoter of neural genes (PubMed:28379941).[UniProtKB/Swiss-Prot Function] |
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