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Tcf12 (NM_011544) Mouse Tagged ORF Clone
CAT＃: MR224159
- TrueORF®
Tcf12 (Myc-DDK-tagged) - Mouse transcription factor 12 (Tcf12)
ORF Plasmid: tGFP
"NM_011544" in other vectors (4)
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CNY 7,768.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A130037E08Rik; ALF1; bHLHb20; HEB; HEBAlt; HTF-4; HTF4; ME1; REB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224159 representing NM_011544
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCCCCAGCAGCAGCGCATGGCCGCGATAGGGACCGACAAGGAGCTGAGCGACCTGCTGGACTTCA GTGCGATGTTTTCTCCACCTGTTAATAGTGGGAAAACGAGACCAACAACACTGGGAAGTAGTCAGTTCAG CGGGTCAGGTATGGATGAGCGTGGAGGAACAACATCTTGGGGAACAAGTGGTCAACCAAGCCCCTCCTAT GATTCATCTAGAGGTTTTACAGACAGCCCTCATTACAGTGATCACTTGAATGACAGTCGATTAGGAACCC ACGAAGGCTTGTCCCCAACACCTTTCATGAACTCAAATCTGATAGGGAAAACATCAGAGAGAGGCTCATT TTCCCTGTACAGCAGAGACTCTGGACTCTCAGGCTGTCAGTCTAGTCTCCTGAGACAAGATCTAGGACTT GGGAGCCCGGCACAGCTGTCTTCTTCAGGAAAGCCTGGAACACCATACTACTCATTCTCTGCCACAAGTT CGAGAAGAAGACCGCTCCATGATTCTGTAGCCCTAGATCCTTTACAAGCGAAGAAAGTAAGAAAGGTGCC TCCTGGCCTACCTTCTTCTGTATATGCACCATCCCCAAATTCTGACGATTTCAACCGTGAATCTCCTAGT TACCCATCTCCCAAGCCACCAACCAGTATGTTCGCTAGCACTTTCTTTATGCAAGATGGGACCCACAGTT CTTCTGACCTTTGGAGTTCATCGAATGGGATGAGCCAGCCTGGTTTTGGTGGAATTCTGGGGACCTCCAC ATCCCACATGTCTCAGTCCAGTAGTTATGGCAGCCTTCATTCACATGACCGCTTGAGTTATCCTCCACAC TCAGTGTCACCAACAGACATAAACACAAGTCTTCCGCCAATGTCCAGCTTCCACCGTGGTAGTACCAGCA GCTCACCATATGTTGCCGCCTCACATACTCCTCCCATCAATGGATCAGATAGCATCCTAGGAACCAGAGG GAATGCTGCTGGAAGCTCACAGACGGGTGATGCACTTGGGAAGGCCTTGGCATCTATTTATTCCCCTGAC CACACAAGCAGTAGTTTTCCATCAAACCCATCAACACCAGTGGGATCTCCTTCACCTCTCACAGGTACCA GTCAGTGGCCCAGAGCTGGAGGGCAAGCTCCTTCATCTCCAAGCTATGAAAACTCACTTCACTCCCTGAA AAATCGAGTTGAGCAGCAACTTCACGAGCATTTGCAAGATGCAATGTCCTTCTTAAAGGATGTCTGTGAG CAGTCTCGAATGGAAGACCGCTTAGACAGGCTGGATGATGCTATCCATGTGCTACGAAACCATGCAGTTG GACCTTCTACCAGTCTGCCTACTAGCCACAGTGACATACACAGTTTGCTGGGACCATCCCATAATGCATC AATTGGAAACCTCAATTCAAACTATGGAGGATCCAGCCTTGTTACAAATAGTCGATCAGCTTCGATGGTC GGAACACATCGGGAAGATTCAGTCAGTCTCAATGGCAATCATTCGGTCCTGTCTAGTACTGTTGCTGCCT CAAACACAGAACTGAACCATAAAACACCAGAAAATTTCAGAGGTGGTGTACAAAATCAGTCTGGAAGTGT TGTTCCAACAGAAATCAAGACTGAAAACAAAGAAAAAGATGAAAACCTTCATGAACCTCCTTCATCAGAT GACATGAAATCAGATGATGAGTCCTCCCAGAAAGACATCAAGGTCTCATCTAGGGGCAGAACAAGCAGTA CCAATGAAGACGAGGATCTGAATCCAGAACAGAAAATCGAAAGGGAGAAGGAAAGGCGGATGGCTAACAA TGCCAGAGAGCGCCTGCGCGTGCGGGATATTAACGAGGCGTTCAAGGAGCTTGGCCGAATGTGTCAGCTT CATTTGAAGAGTGAAAAACCTCAGACAAAACTTCTCATTCTTCATCAGGCCGTGGCAGTCATCCTTAGTC TAGAACAGCAAGTGAGAGAGAGGAACCTCAACCCCAAAGCAGCCTGCCTTAAGAGAAGAGAAGAAGAAAA AGTCTCTGCTGCGTCAGCAGAGCCGCCCAACACGTTGCCAGGAGCCCATCCTGGGCTTAGTGAGTCTACC AACCCTATGGGTCATCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224159 representing NM_011544
Red=Cloning site Green=Tags(s) MNPQQQRMAAIGTDKELSDLLDFSAMFSPPVNSGKTRPTTLGSSQFSGSGMDERGGTTSWGTSGQPSPSY DSSRGFTDSPHYSDHLNDSRLGTHEGLSPTPFMNSNLIGKTSERGSFSLYSRDSGLSGCQSSLLRQDLGL GSPAQLSSSGKPGTPYYSFSATSSRRRPLHDSVALDPLQAKKVRKVPPGLPSSVYAPSPNSDDFNRESPS YPSPKPPTSMFASTFFMQDGTHSSSDLWSSSNGMSQPGFGGILGTSTSHMSQSSSYGSLHSHDRLSYPPH SVSPTDINTSLPPMSSFHRGSTSSSPYVAASHTPPINGSDSILGTRGNAAGSSQTGDALGKALASIYSPD HTSSSFPSNPSTPVGSPSPLTGTSQWPRAGGQAPSSPSYENSLHSLKNRVEQQLHEHLQDAMSFLKDVCE QSRMEDRLDRLDDAIHVLRNHAVGPSTSLPTSHSDIHSLLGPSHNASIGNLNSNYGGSSLVTNSRSASMV GTHREDSVSLNGNHSVLSSTVAASNTELNHKTPENFRGGVQNQSGSVVPTEIKTENKEKDENLHEPPSSD DMKSDDESSQKDIKVSSRGRTSSTNEDEDLNPEQKIEREKERRMANNARERLRVRDINEAFKELGRMCQL HLKSEKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSAASAEPPNTLPGAHPGLSEST NPMGHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011544 |
| ORF Size | 2121 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011544.3, NP_035674.2 |
| RefSeq Size | 4707 bp |
| RefSeq ORF | 2121 bp |
| Locus ID | 21406 |
| UniProt ID | Q61286 |
| MW | 75.8 kDa |
| Gene Summary | Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220764 | Tcf12 (untagged) - Mouse transcription factor 12 (Tcf12), (10ug) |
CNY 9,120.00 |
|
| MG224159 | Tcf12 (tGFP-tagged) - Mouse transcription factor 12 (Tcf12), (10ug) |
CNY 7,700.00 |
|
| MR224159L3 | Lenti ORF clone of Tcf12 (Myc-DDK-tagged) - Mouse transcription factor 12 (Tcf12) |
CNY 8,930.00 |
|
| MR224159L4 | Lenti ORF clone of Tcf12 (mGFP-tagged) - Mouse transcription factor 12 (Tcf12) |
CNY 8,930.00 |
