Mymx (NM_001177470) Mouse Tagged ORF Clone
CAT#: MR224234
- TrueORF®
Gm7325 (Myc-DDK-tagged) - Mouse predicted gene 7325 (Gm7325), transcript variant 3
ORF Plasmid: tGFP
"NM_001177470" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | EG653016; Esgp; Gm7325; minion; myomerger |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR224234 representing NM_001177470
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGAAGAAAGCTGCACTGTAAAACTAATCCAGTTGAAAACTGGGGAGTACAGAGGTGCAGGTCCTG CCATGCCCGTTCCATTGCTCCCGATGGTGCTTCGATCGCTGCTGTCCCGCCTGCTGCTGCCTGTTGCCCG CCTGGCCCGGCAGCACCTCCTGCCCTTGCTGCGCCGGCTGGCCCGCCGACTGAGCTCCCAAGACATGAGA GAGGCTCTGCTGAGCTGTCTGCTCTTTGTCCTCAGCCAGCAACAGCCACCGGATTCTGGAGAGGCCTCCA GAGTGGACCACTCCCAGAGGAAGGAGAGATTGGGCCCCCAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224234 representing NM_001177470
Red=Cloning site Green=Tags(s) MPEESCTVKLIQLKTGEYRGAGPAMPVPLLPMVLRSLLSRLLLPVARLARQHLLPLLRRLARRLSSQDMR EALLSCLLFVLSQQQPPDSGEASRVDHSQRKERLGPQK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001177470 |
ORF Size | 324 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001177470.1, NP_001170941.1 |
RefSeq Size | 852 bp |
RefSeq ORF | 327 bp |
Locus ID | 653016 |
UniProt ID | Q2Q5T5 |
MW | 12.2 kDa |
Gene Summary | Myoblast-specific protein that mediates myoblast fusion, an essential step for the formation of multi-nucleated muscle fibers (PubMed:28386024, PubMed:28569745, PubMed:28569755, PubMed:30197239). Involved in membrane fusion downstream of the lipid mixing step mediated by MYMK (PubMed:30197239). Acts by generating membrane stresses via its extracellular C-terminus, leading to drive fusion pore formation (PubMed:30197239). Acts independently of MYMK (PubMed:30197239). Involved in skeletal muscle regeneration in response to injury by mediating the fusion of satellite cells, a population of muscle stem cells, with injured myofibers (PubMed:29581287).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC215329 | Gm7325 (untagged) - Mouse predicted gene 7325 (Gm7325), transcript variant 3, (10ug) |
CNY 3,990.00 |
|
MG224234 | Gm7325 (tGFP-tagged) - Mouse predicted gene 7325 (Gm7325) transcript variant 3, (10ug) |
CNY 2,800.00 |
|
MR224234L3 | Lenti ORF clone of Gm7325 (Myc-DDK-tagged) - Mouse predicted gene 7325 (Gm7325), transcript variant 3 |
CNY 4,750.00 |
|
MR224234L4 | Lenti ORF clone of Gm7325 (mGFP-tagged) - Mouse predicted gene 7325 (Gm7325), transcript variant 3 |
CNY 4,750.00 |