United States
Germany
Japan
United Kingdom
China
Erap1 (NM_030711) Mouse Tagged Lenti ORF Clone
CAT＃: MR224333L3
- LentiORF®
Lenti ORF clone of Erap1 (Myc-DDK-tagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1)
"NM_030711" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 9,030.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Arts1; ERAAP; PILSA; PILSAP |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR224333).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030711 |
| ORF Size | 2790 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030711.4 |
| RefSeq Size | 4014 bp |
| RefSeq ORF | 2793 bp |
| Locus ID | 80898 |
| UniProt ID | Q9EQH2 |
| Gene Summary | Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222694 | Erap1 (untagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1), (10ug) |
CNY 11,970.00 |
|
| MG224333 | Erap1 (tGFP-tagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1), (10ug) |
CNY 7,890.00 |
|
| MR224333 | Erap1 (Myc-DDK-tagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1) |
CNY 6,808.00 |
|
| MR224333L1 | Lenti ORF clone of Erap1 (Myc-DDK-tagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1) |
CNY 9,030.00 |
|
| MR224333L2 | Lenti ORF clone of Erap1 (mGFP-tagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1) |
CNY 9,208.00 |
|
| MR224333L4 | Lenti ORF clone of Erap1 (mGFP-tagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1) |
CNY 9,030.00 |
