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Jazf1 (NM_173406) Mouse Tagged ORF Clone
CAT＃: MR224470
- TrueORF®
Jazf1 (Myc-DDK-tagged) - Mouse JAZF zinc finger 1 (Jazf1), transcript variant 1
ORF Plasmid: tGFP
"NM_173406" in other vectors (4)
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CNY 2,470.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI591476; C820002C15; Jaz1; Tip27 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224470 representing NM_173406
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGGCATCGCCGCCGCCTCCTTCTTCTCCAACACCTGCCGCTTCGGGGGCTGCGGACTCCACTTCC CCACGCTGGCCGACCTCATCGAGCACATCGAGGACAACCACATCGACACAGACCCACGTGTTTTAGAAAA GCAGGAGCTGCAGCAGCCGACCTATGTGGCCCTCAGTTACATCAATAGATTCATGACAGATGCTGCACGC CGAGAACAGGAATCTCTGAAGAAGAAGATTCAGCCGAAGCTTTCACTAACCCTGTCCAGCTCCGTGTCTC GAGGGAATGTGTCCACTCCACCTCGACATAGCAGTGGCAGCCTTACTCCCCCTGTGACCCCGCCCATCAC GCCCTCCTCTTCATTCCGCAGCAGCACTCCAACAGGCAGCGAGTATGATGAGGAAGAGGTGGACTATGAG GAGTCAGACAGTGATGAGTCCTGGACCACAGAGAGCGCCATCAGCTCTGAAGCAATCCTCAGCTCCATGT GCATGAATGGAGGGGAAGAGAAGCCTTTCGCCTGCCCAGTTCCAGGGTGTAAAAAGAGATACAAGAATGT GAATGGCATAAAGTACCATGCTAAGAATGGTCACCGAACACAGATTCGCGTCCGCAAACCATTCAAATGC CGGTGTGGGAAGAGTTACAAGACAGCTCAGGGCCTGCGGCACCACACAATCAATTTCCATCCCCCAGTGT CTGCCGAGATGATCAGGAAGATGCAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224470 representing NM_173406
Red=Cloning site Green=Tags(s) MTGIAAASFFSNTCRFGGCGLHFPTLADLIEHIEDNHIDTDPRVLEKQELQQPTYVALSYINRFMTDAAR REQESLKKKIQPKLSLTLSSSVSRGNVSTPPRHSSGSLTPPVTPPITPSSSFRSSTPTGSEYDEEEVDYE ESDSDESWTTESAISSEAILSSMCMNGGEEKPFACPVPGCKKRYKNVNGIKYHAKNGHRTQIRVRKPFKC RCGKSYKTAQGLRHHTINFHPPVSAEMIRKMQQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_173406 |
| ORF Size | 729 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_173406.3, NP_775582.2 |
| RefSeq Size | 2925 bp |
| RefSeq ORF | 732 bp |
| Locus ID | 231986 |
| UniProt ID | Q80ZQ5 |
| MW | 27.5 kDa |
| Gene Summary | Acts as a transcriptional corepressor of orphan nuclear receptor NR2C2 (By similarity). Inhibits expression of the gluconeogenesis enzyme PCK2 through inhibition of NR2C2 activity (PubMed:24380856). Also involved in transcriptional activation of NAMPT by promoting expression of PPARA and PPARD (PubMed:24930994). Plays a role in lipid metabolism by suppressing lipogenesis, increasing lipolysis and decreasing lipid accumulation in adipose tissue (PubMed:24380856, PubMed:25614086). Plays a role in glucose homeostasis by improving glucose metabolism and insulin sensitivity (PubMed:25614086, PubMed:24380856).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212934 | Jazf1 (untagged) - Mouse JAZF zinc finger 1 (Jazf1), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MG224470 | Jazf1 (tGFP-tagged) - Mouse JAZF zinc finger 1 (Jazf1) transcript variant 1, (10ug) |
CNY 5,440.00 |
|
| MR224470L3 | Lenti ORF clone of Jazf1 (Myc-DDK-tagged) - Mouse JAZF zinc finger 1 (Jazf1), transcript variant 1 |
CNY 4,370.00 |
|
| MR224470L4 | Lenti ORF clone of Jazf1 (mGFP-tagged) - Mouse JAZF zinc finger 1 (Jazf1), transcript variant 1 |
CNY 4,370.00 |
