Ankrd26 (NM_001081112) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5730521P14Rik; BC008111; mKIAA1074
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR224641 representing NM_001081112 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAGATTTTTGGCTTCAGGAGTAAGGGCCCATCGCCCTTAGGCCCCTCTGCCCGCCCGCGCAGCA ACTGCGTGGGCTTTGGACGCGAGAGTGCTAGTGGCTCCCACGTGCCCAGGTACCATATTCACGACAAGGA TATGGGCAAGATCCACAAGGCTGCAAGCGTGGGAGATGTAGCCAAAGTGCAGCATATCCTTATCCTTGGA AAAAGTGGCGTGAACGATAGAGACAAGAAGGACAGGACTGCTCTACATCTTGCCTGTGCTTACGGCCACC CAGAAGTGGTGACTCTCTTAGTAGAGAGAAAATGTGAGATAGATGCCCGTGACAGCGAGAGCAGCACGGC CCTCATTAAGGCCGTGCAGTGCCAGGAGGAAGAATGTGCAGCCATACTGCTGGACCACGGGGCCGATCCA AATGTGATGGACAGCAGCGGCAACACCGCCCTCCACTACGCGGTCTACAGTGAGAACACGTCCATGGCAG CCAAACTGCTCGCACACAATGCAAACATAGAAGCCAAAAACAAGGATGACCTAACACCTATGTTACTTGC TGTAAAGGAAAATAAACAACATATTGTGGAATTTTTAGTAAAGAAGAAAGCAAGTATTCACGCAGTTGAT CAGCTTGGAAGCAACAGGCAAATGTTTGAATATGATGGGAAGAGACTACAAAGATCTGAAAACAGCAATC CAGTGGATAATGGTTCTGAAGATGGCTCTTTAACCAGGTCTTATAACACACCTGGCCCTGCTGATTCCTG GCCGACATCAGATGAGGAGGACTATAACTTTGACAACAAGAATGTCCCCAAAATTAATTTGACAGAGTTG TGGACAGCTGCTCAGCAGTCTAGGAAAAACCAAACAAAATGTGGCTTTGAGGAGCTAGACAATGGAGCCC GGTTTGATGACAGTGATTCCCCTAGTGAAAGTGAAGATGCCATTGAGGTTGAGCCTGCGCCATCAGTCAG AGTCCAGACCCTCTCCCCATCCCGTCAGTCACCTGACCCTGTGGAAGGAGCAACCGAGCTAGCAATTGAA GGAGAAGAAAACGGTACTGATGTTATTGAGAGCGCTTCACAGGAGCAACCAAATCATGACAATTTAACCC GTGCTGATGGTTGGCACAAAAGTAATAAAAGTGAAATGATGTCTGCATTAGGATTAGGAGAAGACGAAGA TGAACACTCGCCTTGGGATTCTGAGAGTATCTCCGAGAGTGTGTCACTGAAAGACGTAGGTCACTTCTCT GGGACTGCAGATCAAACGGGAAAAAGGAGAGCACATGGACAGATAGAAGATGTGACTTATATACCTTCTT GCATGAGTGGATCAAGAAGCTTTAAAATGGCTAAACTAGAGGAGTCAAGAAATGTAGGCCTTCCGGTAGC CCACATGGAGGCCCCCAGGAAATATGCAATCATGGAGCCTACCATCGAAAGGAGGGCTCCTGTTCTGAAC AAAACGGAGACAGTGGGAATGACGGATGCACAGACTTTCAAGTCAGAGCCAGAATCAGTATCAAGAGAAG AACAGACAAGGCTCAGTGGCAGCGAGGACAGCCAGCAGAAGGTTGAAGAAAAGAGAAAATACAAAAATAA CGAAGCAGAACCCTCAGGAAATCTATACAGCGGTGCTGCTGACGGCGGTGCTGATGTAAAGCCACAGAGT GGAGACACTGAGAACCAGCAGTCTCCCCGGGAGGGGAGTGAGGGACGTGGCAGTGGTCCTGCCTTGCTTA TGAAGGAAGCAAAGAAAATGGAAAATGAAAAATGGGTATCCAGAGAGCCCGCGAGGACGGCAATGAGTGA GCGCACCGGCTTACCAACTGGTGGCTGGCCACAAATGCAGGATGGTTCCTGCTGGAGTGACACGGACCAA AGTGAAGCCAGGCCCACAAAGAAAACATCTAGTAAACATAACAAGGACAGCGGGCAGACAGCTGCAGTGG ACAACCTTGACGACTTTACTGAGTCGTCTGAAACAGCCTCTGAGGACCATGAGCTCCAGGGCCCTGATTC CGAGAGCATTTTGTGTGCGATCGAGCATCTCAGACTGGAGTGTAAAGATACTGCTAGTCTATTGAAAATC CGGGATGCCGTTTATTCATATAAACGATTAATAGAACTTAAAAGAAGCCACTGTGAACTACTTACAGGAA AACTGAAAAGAATGGAAAACAAATATAAGGGATTGCAGAAGGAAATGTCAGAGACAGAAGAAGTGAAGTC ACGGTTGGAACATGAAAAGGTCGGATGGGAACAGGAGCTCTGCAGGCTGAGATTTGCACTGAAACAAGAG GAAGAGAAGAGGAGGAGTGCAGACCAGCTTTCTGAGAAAACCTCGGAGCAGCTTAGAAGGAAAGGCGAGC AGTGTCAGAGTGAGGTGGAAGCCAGGCAGCAGCTTGAAGCCTCTCTCAGAACCCTGGAGATGGAGCTGAA GACGGTGAAAAGCCATCTCAACCAGGTCTTAGAGGAACGAAATGAGACTCAGAGGCAGCTCTCCCGAGAA CAGAATGCCAGAATGTTACAAGATGGGATTCTTGCAAGTCACCTCTGCAAGCAAAAGGAGATAGAAATGA CGCAGAAGAAAATGACTTCTGAGGTTTCTGTTAGTCATGAAAAAGAAAAAGATCTATTGCATAAAAACCA GAGGCTGCAGGATGAAGTTGCTGTGTTACGACTGGAAATGGACACAATAAAAAGCCATAACCAGGAGAAG GAAAAGAGGTATCTTGAGGATATTAAGATTGCAAATGAGAAGAATGACAACCTTCAAAGGATGGTCAAGC TGAATGAAGAAACATTTACAAAAACCATATTCCAGTATACTGGACAGCTTAATAGTCTTAAAGCTGAGAA CACAATGCTGAGTTCTAAGCTGGACAACGAAAAACAAAATAAGGAGAGACTGGAAACAGACGTTGAGTCG TTCCGATCTAGGCTGGCTTCTGCTCTGCATGATCACGCTGAAATTCAGACAGCAAAAAGAGACCTAGAAA TTGCTTTCCAGAGAGCGAGAGATGAGTGGTTTCGTGTGAAAGACAAGATGAATTTCGATATGTCTAACCT CCGAGATAACAATGAGGTTCTCTCCCAACAGCTTTCTAAAACTGAAAGGAAGTTGAATAGCCTAGAAATT GAGTTTCATCACACGAAAGATGAGCTTCGAGAGAAGACGCTGGCGTTAAAGCATGCGCAGAGAGACCTGA GCCAGACCCAGTGCCAGATGAAGGAGGTGGAGCACATGTTTCAGGACGAACAAGGCAAAGTGAGCAAGTT CATGGGGAAGCAGGAGTCCATAGAGGAGAGGCTGGCCCAGCTGCAGAGTGAGAACACGCTGCTCCGGCAG CAGCTGGACGACGCTGCCAACAAGGCCGAGAGCAAAGACAAGACCATAGTCAACATCCAAGACCAGTTCC AGGATGTGCTAACCAGGTTCCAAGCTGAGAGTCAGCGGCACAGCCTGAGGCTAGAAGACAGAAACCAGGA GTTAGTCAGTGAGTGCAGCCATCTAAGAGAAAGACTGTGTCAGTACGAGAACGAGAAAGCCGAAAGAGAG GTAGTTGTGAGGCAACTCCAGCAAGAATTGGCTGATACCCTAAAAAAGCAGTCGATGTCAGAGGCTTCGC TGGAAGTCTCCTCCCGCTACCGCAGCAATTTAGAAGAAGAGGCGCGAGACTTAAAGAAGAAATTAGGTCA GCTCAGAAGTCAGTTGCAGGAGGCGCGGGATCAACACAGAGAGGCTGTACACCATGCTGAGAAGATGGAA GACCACCTACAAAAGCTTGAACTTGAAAAATCCAAGTTCGAAATTACAATTAAAAAGCAATCAGAGGAAA TTGATCAACTTCAGGAAAACCTGTCAAGGGTGAATTTGTCTGAAGAGGATAAGGAAAAACTGCAGAAACT TACCGAGTTGAAGGAGTCTCTGGAATGTACTGTGGATCAAGAACAGAAGAGAAGCAGTGCGTTAGAAAAA GAACTGGCAGAATTTAAGGAAGTCTTAAAAATGACCAAAAAAGAATTAAATGAATATGAAAACAGAGAAC TTAATCTTCGTCAAGATATAAAAAACAATCACCTTGAAATGGATATCCCAGTTAGTACACTAATAAAAAA GATTGATGATCTTACAGCAAAACTGGAAACTGCATCTTCAAAATGTCTACATCTGGGTAAAAAAAATCAG CTGCTTCAGCAGGAGTTACTATTGATGAGGACTATACAAAAGAAGTGTGGGAAACTAGAGAAGAATAAAA AGCAATTGGAGCAGGAGGTCGTGAACCTCAGAAGTCACATGGAGAAGAACATGGTGGAGCACAGCCAAGC ACAGCAGTACGCGCGGGAGGTCGAAGAGCGAGCGAGGCAGGACTTGGTGGAAAAGTTAAAACAGGTCAAC CTCTTCCTGCAGGCTCAAGCAGCATCCCAGGAGAGCTTGGAGCAGTTAAGAGAGAACAGTAACGCTTCGG TTAGAAGTCAGATGGAGCTCAGGATTAAAGACCTGGAGTCCCAGCTCTACAGAATGAAAGCCCAGGAAGA CTTCGATAAAATAGAACTGGAAAAGTATAAGCAGCTCTACCAGGAAGAGTTCAGAGCTAGAAAATCGCTG TCAAGTAAACTAAACAAGACCAGTGAGAAGCTGGAGGAGGCCAGCAGCAAGCTGCTCCTGGAGGAGCAGC AGAACAGGTCCTTGCTGAGCACTCTGAGTACAAGGCCTGTTGTGGAGTGTCCTTGTGTTGGAAGTTTCCA TAATAGTTTGGTATTCAACAGAACTCTTATTCCAAGAGAAAACATAGTGGTTCCTACTTCAGGCCTACAG CCATCAAATAAGAGAGTGGAGATCTACCTGACCAAGATGCATCAAGAGTTGGAAAAAAGTATAAATAGAG AACTTAAAGAAGCTACTGCTGAACTTGAATCTGAGTTCTGCAGAGTTTCTCCCCTGGGATCTGCTACTAA AGCAAGCCAAGATCAACTCTCAGACGCATCGCAAGAATTTATAGACATTCTAAAGAAAAAATATATGATC TGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR224641 representing NM_001081112 Red=Cloning site Green=Tags(s) MKKIFGFRSKGPSPLGPSARPRSNCVGFGRESASGSHVPRYHIHDKDMGKIHKAASVGDVAKVQHILILG KSGVNDRDKKDRTALHLACAYGHPEVVTLLVERKCEIDARDSESSTALIKAVQCQEEECAAILLDHGADP NVMDSSGNTALHYAVYSENTSMAAKLLAHNANIEAKNKDDLTPMLLAVKENKQHIVEFLVKKKASIHAVD QLGSNRQMFEYDGKRLQRSENSNPVDNGSEDGSLTRSYNTPGPADSWPTSDEEDYNFDNKNVPKINLTEL WTAAQQSRKNQTKCGFEELDNGARFDDSDSPSESEDAIEVEPAPSVRVQTLSPSRQSPDPVEGATELAIE GEENGTDVIESASQEQPNHDNLTRADGWHKSNKSEMMSALGLGEDEDEHSPWDSESISESVSLKDVGHFS GTADQTGKRRAHGQIEDVTYIPSCMSGSRSFKMAKLEESRNVGLPVAHMEAPRKYAIMEPTIERRAPVLN KTETVGMTDAQTFKSEPESVSREEQTRLSGSEDSQQKVEEKRKYKNNEAEPSGNLYSGAADGGADVKPQS GDTENQQSPREGSEGRGSGPALLMKEAKKMENEKWVSREPARTAMSERTGLPTGGWPQMQDGSCWSDTDQ SEARPTKKTSSKHNKDSGQTAAVDNLDDFTESSETASEDHELQGPDSESILCAIEHLRLECKDTASLLKI RDAVYSYKRLIELKRSHCELLTGKLKRMENKYKGLQKEMSETEEVKSRLEHEKVGWEQELCRLRFALKQE EEKRRSADQLSEKTSEQLRRKGEQCQSEVEARQQLEASLRTLEMELKTVKSHLNQVLEERNETQRQLSRE QNARMLQDGILASHLCKQKEIEMTQKKMTSEVSVSHEKEKDLLHKNQRLQDEVAVLRLEMDTIKSHNQEK EKRYLEDIKIANEKNDNLQRMVKLNEETFTKTIFQYTGQLNSLKAENTMLSSKLDNEKQNKERLETDVES FRSRLASALHDHAEIQTAKRDLEIAFQRARDEWFRVKDKMNFDMSNLRDNNEVLSQQLSKTERKLNSLEI EFHHTKDELREKTLALKHAQRDLSQTQCQMKEVEHMFQDEQGKVSKFMGKQESIEERLAQLQSENTLLRQ QLDDAANKAESKDKTIVNIQDQFQDVLTRFQAESQRHSLRLEDRNQELVSECSHLRERLCQYENEKAERE VVVRQLQQELADTLKKQSMSEASLEVSSRYRSNLEEEARDLKKKLGQLRSQLQEARDQHREAVHHAEKME DHLQKLELEKSKFEITIKKQSEEIDQLQENLSRVNLSEEDKEKLQKLTELKESLECTVDQEQKRSSALEK ELAEFKEVLKMTKKELNEYENRELNLRQDIKNNHLEMDIPVSTLIKKIDDLTAKLETASSKCLHLGKKNQ LLQQELLLMRTIQKKCGKLEKNKKQLEQEVVNLRSHMEKNMVEHSQAQQYAREVEERARQDLVEKLKQVN LFLQAQAASQESLEQLRENSNASVRSQMELRIKDLESQLYRMKAQEDFDKIELEKYKQLYQEEFRARKSL SSKLNKTSEKLEEASSKLLLEEQQNRSLLSTLSTRPVVECPCVGSFHNSLVFNRTLIPRENIVVPTSGLQ PSNKRVEIYLTKMHQELEKSINRELKEATAELESEFCRVSPLGSATKASQDQLSDASQEFIDILKKKYMI C TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001081112
ORF Size	5043 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001081112.2
RefSeq Size	5704 bp
RefSeq ORF	5046 bp
Locus ID	232339
MW	192.2 kDa

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