Mink1 (NM_016713) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	B55; Map4k6; MINK; Ysk2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR224719 representing NM_016713 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACCCAGCCCCCGCCCGCAGCCTGGACGACATCGACCTGTCTGCCCTGCGGGACCCTGCAGGAA TCTTTGAGCTGGTGGAGGTGGTTGGCAATGGAACCTATGGACAGGTATACAAGGGGCGGCACGTCAAGAC TGGGCAGCTGGCTGCCATTAAGGTCATGGATGTCACAGAGGATGAGGAGGAAGAGATCAAACAGGAAATC AACATGTTAAAGAAGTACTCTCACCATCGCAATATTGCCACCTACTATGGGGCCTTTATCAAGAAGAGCC CTCCTGGGAACGATGACCAGCTCTGGCTGGTGATGGAGTTCTGCGGTGCTGGTTCAGTGACCGACCTGGT AAAGAACACAAAAGGGAACGCACTGAAGGAGGATTGCATTGCTTACATCTGCAGGGAGATTCTCAGGGGT CTTGCCCATCTCCATGCCCACAAGGTGATCCACAGAGATATCAAGGGACAAAATGTGCTGCTGACAGAGA ATGCTGAAGTCAAGCTAGTGGATTTTGGGGTGAGTGCTCAGCTGGACCGCACTGTGGGCAGGCGGAACAC TTTCATTGGAACCCCATACTGGATGGCTCCAGAAGTCATTGCCTGTGACGAGAACCCCGATGCCACCTAT GACTACAGGAGTGACATTTGGTCTCTAGGAATCACAGCCATTGAAATGGCAGAGGGAGCCCCCCCTCTGT GTGACATGCACCCTATGCGGGCCCTCTTCCTCATCCCTCGGAACCCTCCCCCCAGGCTCAAGTCAAAGAA ATGGTCTAAGAAGTTCACTGACTTCATCGACACGTGTCTCATCAAGACTTACCTGAGCCGCCCACCCACC GAACAGTTACTCAAATTCCCCTTCATCCGAGACCAGCCCACGGAGCGGCAGGTCCGCATCCAGCTCAAGG ACCACATCGACCGCTCGCGGAAGAAGCGGGGTGAGAAAGAGGAGACAGAGTATGAGTACAGCGGCAGTGA GGAGGAAGACGACAGCCATGGAGAGGAAGGCGAGCCAAGCTCCATCATGAATGTGCCCGGTGAGTCCACA CTGCGCAGAGAATTCCTCAGACTCCAGCAGGAGAATAAGAGCAACTCTGAGGCTTTAAAGCAGCAGCAGC AGCTGCAGCAACAGCAGCAGCGGGACCCGGAGGCACACATCAAACACCTGCTGCACCAGCGGCAGCGTCG CATAGAGGAGCAGAAGGAGGAGCGGCGACGTGTGGAGGAGCAACAGCGGCGAGAGCGAGAACAGCGTAAG CTACAAGAGAAGGAGCAGCAGCGGCGATTGGAAGACATGCAAGCCCTACGACGAGAGGAAGAGAGGCGGC AAGCAGAGCGGGAACAGGAATACAAGCGGAAGCAGCTGGAGGAGCAGCGGCAGTCAGAGCGGCTGCAGAG ACAGCTGCAGCAGGAGCACGCCTACCTCAAGTCCCTGCAGCAGCAGCAGCAGCAGCAGCAGCTCCAGAAG CAGCAGCAGCAGCAGCAGCAGATCCTGCCTGGAGACAGGAAGCCCCTGTATCATTACGGTCGGGGCATTA ATCCTGCTGACAAGCCAGCATGGGCCCGCGAGGTGGAAGAGAGAGCACGGATGAACAAGCAGCAGAACTC TCCCTTGGCGAAGGCGAAGCCAAGCAGTGCGGGGCCAGAGCCCCCCATCTCCCAGGCCTCTCCTAGCCCC CCAGGACCTCTTTCCCAGACTCCTCCTATGCAGAGGCCTGTGGAGCCCCAGGAAGGACCGCACAAGAGCC TGGTGGCACACCGGGTCCCACTGAAGCCATATGCAGCACCTGTACCCCGATCCCAGTCCCTGCAGGACCA GCCGACTCGAAACCTGGCTGCCTTCCCAGCCTCCCACGACCCTGACCCTGCTGCTGTCCCTACACCCACT GCCACACCCAGTGCCCGAGGAGCTGTCATCCGCCAGAATTCAGACCCCACCTCTGAAGGGCCAGGGCCTA GCCCAAACCCTCCATCCTGGGTTCGGCCTGATAATGAGGCTCCACCTAAGGTTCCACAGAGGACCTCTTC TATCGCCACTGCCCTTAACACCAGTGGGGCCGGAGGGTCCCGGCCAGCTCAGGCTGTCCGTGCCAGTAAC CCTGACCTCAGGAGGAGTGACCCTGGCTGGGAGCGCTCAGACAGTGTCCTCCCGGCCTCCCACGGCCACC TCCCTCAGGCTGGCTCCTTGGAGCGGAACCGAAACCGTGTGGGAGCCTCCACAAAACTGGATAGCTCTCC AGTGCTCTCCCCTGGGAACAAAGCCAAGCCTGAAGACCACCGCTCAAGGCCAGGCCGGCCCGCAGACTTT GTGTTGCTCAAAGAGCGGACTCTGGATGAGGCCCCTAAGCCTCCCAAGAAGGCCATGGACTACTCCTCAT CCAGTGAGGAGGTGGAAAGCAGTGAAGAGGAGGAGGAGGAAGGCGATGGGGAGCCGTCAGAGGGGAGCAG AGACACTCCCGGGGGCCGCAGTGATGGTGATACAGACAGCGTCAGCACCATGGTGGTTCATGATGTTGAG GAGATATCCGGGACCCAGCCCTCATATGGCGGCGGCACCATGGTGGTCCAGCGTACTCCTGAAGAGGAAC GAAGCCTGCTGCTTGCTGATAGCAATGGCTACACAAACCTGCCTGATGTGGTCCAGCCCAGCCACTCACC TACTGAGAACAGCAAAGGTCAAAGCCCTCCAACAAAGGATGGAGGCAGTGATTACCAGTCTCGTGGGCTG GTAAAGGCCCCAGGAAAGAGCTCATTCACCATGTTTGTGGATCTAGGGATCTACCAGCCTGGAGGCAGTG GGGACACCATCCCTATCACAGCCCTAGTGGGTGGAGAAGGTGGTCGCCTTGATCAACTGCAGTTCGATGT GAGGAAGGGCTCTGTGGTCAACGTCAATCCCACCAACACCCGAGCTCATAGTGAAACTCCTGAAATTCGC AAGTACAAGAAGCGATTCAACTCAGAGATCCTATGTGCAGCTCTCTGGGGGGTCAACCTCCTAGTGGGCA CAGAGAATGGGCTGATGTTGCTGGACCGAAGTGGGCAGGGCAAGGTGTATGGACTTATTGGGCGACGACG CTTCCAGCAAATGGATGTCTTAGAAGGGCTCAACTTGCTCATCACCATCTCAGGGAAAAGGAACAAACTG CGGGTATATTACCTGTCCTGGCTTCGGAACAAGATCCTACACAATGACCCAGAGGTGGAAAAGAAGCAGG GGTGGACCACCGTGGGGGACATGGAGGGCTGCGGCCACTACCGTGTTGTGAAATATGAACGGATTAAGTT CCTGGTCATTGCCCTGAAGAACTCCGTGGAGGTTTATGCCTGGGCTCCCAAACCCTACCACAAATTCATG GCCTTCAAGTCCTTTGCTGACCTCCCTCACCGCCCTCTACTGGTGGACCTGACAGTAGAGGAGGGACAGC GGCTCAAGGTCATCTATGGCTCCAGTGCTGGCTTCCATGCTGTGGATGTTGATTCTGGGAACAGCTATGA CATCTACATCCCTGTACATATCCAGAGCCAGATCACACCCCACGCCATCATCTTCCTCCCCAACACTGAT GGCATGGAGATGCTGCTGTGCTATGAAGATGAGGGTGTCTATGTCAACACTTACGGGCGGATCATCAAGG ATGTGGTGCTGCAGTGGGGAGAGATGCCCACCTCTGTGGCCTACATCTGCTCCAACCAGATAATGGGCTG GGGTGAGAAGGCCATAGAGATCCGCTCTGTGGAGACAGGCCACCTAGATGGGGTCTTCATGCACAAACGA GCCCAGAGGCTCAAGTTCCTGTGTGAGCGCAATGACAAGGTGTTTTTTGCCTCTGTCCGCTCTGGAGGAA GCAGCCAAGTTTACTTTATGACTCTGAACCGTAACTGCATCATGAACTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR224719 representing NM_016713 Red=Cloning site Green=Tags(s) MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEI NMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRG LAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATY DYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPT EQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGEST LRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRK LQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQK QQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSP PGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPT ATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASN PDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPADF VLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVE EISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGL VKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIR KYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKL RVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFM AFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTD GMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKR AQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_016713
ORF Size	3900 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_016713.2, NP_057922.2
RefSeq Size	4862 bp
RefSeq ORF	3903 bp
Locus ID	50932
UniProt ID	Q9JM52
MW	146.8 kDa
Gene Summary	Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.