Myoc (NM_010865) Mouse Tagged ORF Clone
CAT#: MR224777
- TrueORF®
Myoc (Myc-DDK-tagged) - Mouse myocilin (Myoc)
ORF Plasmid: tGFP
"NM_010865" in other vectors (4)
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CNY 3,656.00
CNY 4,280.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI957332; GLC1A; TIGR |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR224777 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCTCTCCATCTGCTGTTTCTGGCCTGCTTGGTGTGGGGAATGGGGGCCAGGACAGCACAGTTCC GAAAGGCCAATGATCGGAGTGGCCGATGCCAATACACCTTCACTGTGGCCAGCCCCAATGAATCTAGCTG CCCAAGGGAGGACCAGGCCATGTCAGCCATCCAAGACCTTCAGAGAGACAGCAGCATCCAGCATGCAGAC CTAGAGTCCACCAAGGCCCGGGTCAGATCCCTGGAGAGTCTCCTCCACCAGATGACCTTGGGCCGAGTTA CTGGGACCCAGGAGGCCCAAGAGGGGCTGCAGGGCCAGTTGGGTGCCCTGAGGAGAGAACGGGACCAGCT GGAGACCCAAACCAGGGATCTGGAGGCAGCCTATAACAATCTCCTTCGAGATAAGTCGGCTTTAGAGGAA GAGAAGAGGCAGCTGGAACAAGAGAATGAAGATTTGGCCAGGAGGCTAGAAAGCAGCAGCGAGGAGGTAA CAAGGCTGCGGAGGGGCCAGTGTCCTTCCACCCAGTACCCCTCTCAGGACATGCTGCCAGGCTCCAGGGA AGTCTCTCAGTGGAATTTGGACACGTTGGCCTTCCAGGAATTGAAGTCAGAGTTAACTGAGGTTCCTGCT TCCCAAATCTTGAAGGAAAATCCATCTGGCCGACCCAGGAGCAAAGAAGGAGACAAAGGATGTGGAGCGC TAGTCTGGGTAGGAGAGCCAGTCACCCTGAGGACAGCTGAAACAATCGCTGGCAAGTATGGAGTGTGGAT GAGAGACCCCAAGCCCACCCACCCCTACACCCAGGAAAGCACATGGAGGATTGACACGGTTGGCACAGAG ATCCGCCAGGTGTTTGAGTACAGTCAGATAAGCCAGTTCGAGCAGGGCTATCCTTCCAAGGTCCATGTGC TCCCTCGGGCACTGGAGAGCACGGGTGCTGTGGTGTATGCGGGGAGCCTCTATTTCCAGGGGGCTGAGTC CAGAACTGTGGTCAGGTATGAGCTAGACACGGAGACCGTGAAGGCAGAGAAGGAAATTCCTGGAGCTGGC TACCACGGACACTTCCCGTACGCGTGGGGTGGCTACACAGACATTGACTTAGCTGTGGATGAGAGCGGCC TCTGGGTCATCTACAGCACGGAGGAAGCCAAGGGGGCCATAGTCCTCTCCAAATTGAACCCAGCGAACCT GGAACTTGAGCGTACCTGGGAGACTAACATCCGTAAGCAGTCTGTGGCCAATGCCTTTGTTATCTGTGGC ATCTTGTACACGGTGAGCAGCTACTCTTCAGCCCATGCAACCGTCAACTTCGCCTACGACACTAAAACGG GGACCAGTAAGACCCTGACCATCCCATTCACGAATCGCTACAAGTACAGCAGTATGATTGACTACAACCC CCTGGAGAGGAAGCTGTTTGCCTGGGACAACTTCAACATGGTCACCTATGATATCAAGCTCTTGGAGATG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR224777 protein sequence
Red=Cloning site Green=Tags(s) MPALHLLFLACLVWGMGARTAQFRKANDRSGRCQYTFTVASPNESSCPREDQAMSAIQDLQRDSSIQHAD LESTKARVRSLESLLHQMTLGRVTGTQEAQEGLQGQLGALRRERDQLETQTRDLEAAYNNLLRDKSALEE EKRQLEQENEDLARRLESSSEEVTRLRRGQCPSTQYPSQDMLPGSREVSQWNLDTLAFQELKSELTEVPA SQILKENPSGRPRSKEGDKGCGALVWVGEPVTLRTAETIAGKYGVWMRDPKPTHPYTQESTWRIDTVGTE IRQVFEYSQISQFEQGYPSKVHVLPRALESTGAVVYAGSLYFQGAESRTVVRYELDTETVKAEKEIPGAG YHGHFPYAWGGYTDIDLAVDESGLWVIYSTEEAKGAIVLSKLNPANLELERTWETNIRKQSVANAFVICG ILYTVSSYSSAHATVNFAYDTKTGTSKTLTIPFTNRYKYSSMIDYNPLERKLFAWDNFNMVTYDIKLLEM SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010865 |
ORF Size | 1473 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010865.3, NP_034995.3 |
RefSeq Size | 2093 bp |
RefSeq ORF | 1473 bp |
Locus ID | 17926 |
UniProt ID | O70624 |
MW | 55.3 kDa |
Gene Summary | Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction. Modulates the organization of actin cytoskeleton by stimulating the formation of stress fibers through interactions with components of Wnt signaling pathways. Promotes cell migration through activation of PTK2 and the downstream phosphatidylinositol 3-kinase signaling (By similarity). Plays a role in bone formation and promotes osteoblast differentiation in a dose-dependent manner through mitogen-activated protein kinase signaling (PubMed:23629661). Mediates myelination in the peripheral nervous system through ERBB2/ERBB3 signaling (PubMed:23897819). Plays a role as a regulator of muscle hypertrophy through the components of dystrophin-associated protein complex (PubMed:22371502). Involved in positive regulation of mitochondrial depolarization. Plays a role in neurite outgrowth. May participate in the obstruction of fluid outflow in the trabecular meshwork (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Role of truncated oxidized phospholipids in acute endothelial barrier dysfunction caused by particulate matter
,Karki, P;Meliton, A;Shah, A;Tian, Y;Ohmura, T;Sarich, N;Birukova, AA;Birukov, KG;,
PLoS ONE
,PubMed ID 30419037
[MYOC]
|
Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle
,Lynch, JM;Dolman, AJ;Guo, C;Dolan, K;Xiang, C;Reda, S;Li, B;Prasanna, G;,
PLoS ONE
,PubMed ID 30395621
[MYOC]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216807 | Myoc (untagged) - Mouse myocilin (Myoc), (10ug) |
CNY 3,656.00 |
|
MG224777 | Myoc (tGFP-tagged) - Mouse myocilin (Myoc), (10ug) |
CNY 4,750.00 |
|
MR224777L3 | Lenti ORF clone of Myoc (Myc-DDK-tagged) - Mouse myocilin (Myoc) |
CNY 6,180.00 |
|
MR224777L4 | Lenti ORF clone of Myoc (mGFP-tagged) - Mouse myocilin (Myoc) |
CNY 6,180.00 |