Tnnt2 (NM_001130178) Mouse Tagged ORF Clone
CAT#: MR224788
- TrueORF®
Tnnt2 (Myc-DDK-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 5
ORF Plasmid: tGFP
"NM_001130178" in other vectors (4)
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CNY 2,950.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | cTnT; Tnt |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR224788 representing NM_001130178
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACGCCGAGGAGGTGGTGGAGGAGTACGAGGAGGAACAGGAAGAAGCTGTGGAAGAGCAAGAGG AGGCAGTGGAGGAGGAGGAGGCTGGTGGGGCTGAACCTGAGCCTGAGGGTGAGGCTGAGACAGAGGAGGC CAACGTAGAAGAGGTTGGTCCTGATGAAGAAGCCAAAGATGCTGAAGAAGGTCCAGTAGAGGACACCAAA CCCAAGCCCAGCAGGCTCTTCATGCCCAACTTGGTGCCACCCAAGATCCCCGATGGAGAGAGAGTGGACT TTGATGACATCCACAGGAAGCGCGTGGAGAAGGACCTGAATGAGCTACAGACTCTGATCGAGGCTCACTT CGAGAACAGGAAGAAGGAGGAAGAGGAGCTGATTTCCCTCAAAGACAGGATCGAAAAGCGTCGGGCAGAG CGGGCCGAGCAGCAGCGTATTCGCAATGAGCGGGAGAAGGAAAGGCAGAACCGCCTGGCTGAAGAGAGGG CCCGGCGTGAGGAGGAGGAGAACAGGAGGAAGGCTGAGGATGAGGCCCGGAAGAAGAAGGCTCTGTCCAA CATGATGCACTTTGGAGGGTACATCCAGAAGCAAGCTCAGACAGAGCGGAAGAGTGGGAAGAGACAGACA GAGAGAGAGAAGAAGAAGAAGATCCTGGCAGAGAGGAGGAAGGCGCTGGCCATCGACCACCTGAATGAAG ACCAACTGAGAGAGAAGGCCAAGGAGCTGTGGCAGAGTATTCACAACCTGGAGGCTGAGAAGTTCGACCT GCAGGAAAAGTTCAAGCAGCAGAAATACGAAATCAACGTTCTGCGAAACCGGATCAATGACAACCAGAAA GTCTCCAAAACTCGTGGGAAGGCCAAAGTCACCGGGCGTTGGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224788 representing NM_001130178
Red=Cloning site Green=Tags(s) MSDAEEVVEEYEEEQEEAVEEQEEAVEEEEAGGAEPEPEGEAETEEANVEEVGPDEEAKDAEEGPVEDTK PKPSRLFMPNLVPPKIPDGERVDFDDIHRKRVEKDLNELQTLIEAHFENRKKEEEELISLKDRIEKRRAE RAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKQAQTERKSGKRQT EREKKKKILAERRKALAIDHLNEDQLREKAKELWQSIHNLEAEKFDLQEKFKQQKYEINVLRNRINDNQK VSKTRGKAKVTGRWK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001130178 |
ORF Size | 885 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001130178.2, NP_001123650.1 |
RefSeq Size | 1181 bp |
RefSeq ORF | 888 bp |
Locus ID | 21956 |
UniProt ID | P50752 |
MW | 35.4 kDa |
Gene Summary | Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209558 | Tnnt2 (untagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 5, (10ug) |
CNY 3,990.00 |
|
MG224788 | Tnnt2 (tGFP-tagged) - Mouse troponin T2 cardiac (Tnnt2) transcript variant 5, (10ug) |
CNY 3,230.00 |
|
MR224788L3 | Lenti ORF clone of Tnnt2 (Myc-DDK-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 5 |
CNY 4,850.00 |
|
MR224788L4 | Lenti ORF clone of Tnnt2 (mGFP-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 5 |
CNY 4,850.00 |