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Deaf1 (NM_016874) Mouse Tagged ORF Clone
CAT＃: MR224847
- TrueORF®
Deaf1 (Myc-DDK-tagged) - Mouse deformed epidermal autoregulatory factor 1 (Drosophila) (Deaf1), transcript variant 1
ORF Plasmid: tGFP
"NM_016874" in other vectors (4)
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CNY 4,216.00
CNY 5,040.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AU042387; C230009B13Rik; NUDR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224847 representing NM_016874
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACTCGGACTCGGCGGCAAAACAGCTGGGCCTGGCCGAGGCGGCAGCTGTGGCGGCCGCTGCCG CGGTGGCGGCGGCCGCCGCGGCCGCAGCGGAAAGCGAGGCGGAGGAGCCGGTGTTAAGCAGAGACGAGGA CTCGGAGGAGGACGCAGACTCCGAGGCGGAGCGCGAGACGCGGCGGGTCACCGCTGTGGCGGTGATGGCG GCCGAATCTGGGCACATGGATATGGGTACCGAGGCTCTGCCCAGCCCCGATGAGGCCGCCGCCGCCGCCG CCGCCTTCGCAGAAGTGACCACAGTGACTGTGGCCAACGTGGGGTCCTCTGCAGATAACGTCTTCACAAC ATCAGTGGCAAACGCAGCATCGATATCAGGACATGTCCTGTCTGGTAGGACAGCCCTGCAGATCGGTGAC AGCCTGAACACTGAAAAAGCCACACTAATAGTTGTACACACAGATGGGAGCATTGTGGAGACCACTGGGC TGAAGGGCCCAGCAGCACCTCTCACTCCAGGTCCCCAGTCTCCTCCTACCCCACTGGCACCTGGCCAGGA GAAAGGTGGTACCAAGTACAACTGGGACCCTTCGGTGTATGACAGCGAGTTGCCTGTGCGCTGTCGGAAC ATCAGTGGCACGCTCTACAAGAGTAGGCTCGGCTCAGGTGGCCGGGGCCGGTGTATCAAGCAGGGAGAAA ACTGGTACAGCCCAACTGAGTTTGAAGCTATGGCAGGAAGAGCCAGCAGCAAGGACTGGAAGAGAAGCAT CCGCTATGCTGGCAGACCCCTGCAGTGCCTCATTCAGGATGGTATTTTGAACCCTCACGCTGCCTCCTGT ACCTGTGCCGCCTGTTGTGATGACATGACTCTGAGTGGCCCTGTCAGGCTCTTCGTCCCTTATAAAAGGC GCAAGAAAGAGAATGAGCTGCCCACAACTCCAGTGAAGAAGGATTCCCCCAAGAACATCACCCTGCTTCC TGCCACGGCGGCCACCACCTTCACTGTGACACCCTCAGGACAGATCACTACCTCTGGAGCACTGACCTTT GACAGAGCATCCACTGTAGAGGCCACTGCTGTCATCTCTGAGAGCCCAGCCCAAGGTGATGTCTTTGCAG GAGCCACAGTGCAAGAGGCAGGTGTGCAGCCTCCCTGCAGGGTTGGCCACCCTGAACCCCACTACCCTGG CTATCAGGACAGCTGCCAGATTGCCCCGTTTCCAGAAGCTGCATTGCCAACATCACACCCCAAAATTGTC CTGACATCGCTGCCCGCATTGGCCGTGCCACCGTCCACCCCCACCAAAGCTGTCTCTCCCACCGTGGTCA GTGGGCTGGAGATGTCAGAACATCGGAGCTGGCTGTACCTGGAAGAGATGGTCAACTCCCTACTCAACAC AGCTCAGCAGCTGAAGACGCTGTTTGAACAAGCCAAGCAGGCGAGCTCTTGCAGGGAAGCTGCTGTGACC CAGGCGAGAATGCAGGTTGATACAGAGAGGAAAGAGCAGTCATGTGTCAACTGCGGCCGGGAGGCCATGA GTGAGTGTACCGGCTGCCACAAGGTTAACTACTGCTCTACGTTCTGCCAGCGCAAGGACTGGAAAGACCA TCAGCATGTGTGTGGCCAGTCAGCGTCTGTCACTGTCCAGGCTGATGACGTCCATGTTGAAGAAAGTGTG ATAGAAAAAGTTGCTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224847 representing NM_016874
Red=Cloning site Green=Tags(s) MEDSDSAAKQLGLAEAAAVAAAAAVAAAAAAAAESEAEEPVLSRDEDSEEDADSEAERETRRVTAVAVMA AESGHMDMGTEALPSPDEAAAAAAAFAEVTTVTVANVGSSADNVFTTSVANAASISGHVLSGRTALQIGD SLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRN ISGTLYKSRLGSGGRGRCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPHAASC TCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLLPATAATTFTVTPSGQITTSGALTF DRASTVEATAVISESPAQGDVFAGATVQEAGVQPPCRVGHPEPHYPGYQDSCQIAPFPEAALPTSHPKIV LTSLPALAVPPSTPTKAVSPTVVSGLEMSEHRSWLYLEEMVNSLLNTAQQLKTLFEQAKQASSCREAAVT QARMQVDTERKEQSCVNCGREAMSECTGCHKVNYCSTFCQRKDWKDHQHVCGQSASVTVQADDVHVEESV IEKVAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016874 |
| ORF Size | 1698 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016874.3, NP_058570.1 |
| RefSeq Size | 2192 bp |
| RefSeq ORF | 1701 bp |
| Locus ID | 54006 |
| UniProt ID | Q9Z1T5 |
| MW | 60.1 kDa |
| Gene Summary | Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction (By similarity). Regulates epithelial cell proliferation and side-branching in the mammary gland. Required for neural tube closure and skeletal patterning. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 2. Isoform 2 may inhibit transcriptional activity of isoform 1 by interacting with it and retaining it in the cytoplasm. Transcriptional activator of EIF4G3 (By similarity). May also involved in behavior (PubMed:24726472).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219069 | Deaf1 (untagged) - Mouse deformed epidermal autoregulatory factor 1 (Drosophila) (Deaf1), transcript variant 1, (10ug) |
CNY 4,648.00 |
|
| MG224847 | Deaf1 (tGFP-tagged) - Mouse deformed epidermal autoregulatory factor 1 (Drosophila) (Deaf1) transcript variant 1, (10ug) |
CNY 5,510.00 |
|
| MR224847L3 | Lenti ORF clone of Deaf1 (Myc-DDK-tagged) - Mouse deformed epidermal autoregulatory factor 1 (Drosophila) (Deaf1), transcript variant 1 |
CNY 6,940.00 |
|
| MR224847L4 | Lenti ORF clone of Deaf1 (mGFP-tagged) - Mouse deformed epidermal autoregulatory factor 1 (Drosophila) (Deaf1), transcript variant 1 |
CNY 6,940.00 |
