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Kcnu1 (NM_008432) Mouse Tagged ORF Clone
CAT＃: MR224899
- TrueORF®
Kcnu1 (Myc-DDK-tagged) - Mouse potassium channel, subfamily U, member 1 (Kcnu1)
ORF Plasmid: tGFP
"NM_008432" in other vectors (4)
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CNY 7,808.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Kcnma3; mSlo3; Slo3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224899 representing NM_008432
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCAAACATTGCTAGACAGTTTAAATCAGAAGGAGTTGACGGAAACGTCATGTACAATCGAAATCC AGGCAGCGTTCATTCTTTCCTCCTTGGCGACTTTCTTCGGGGGACTCATCATCTTATTCCTTTTCAGAAT AGCCTTGAAAAGCTCAAGAAGTTGGAAATACGTCAAGGGGCCAAGAGGACTCTTGGAACTATTCTCATCA CGTAGAATCGAGGCTAATCCTTTGAGGAAACTTTACTTTCATGGAGTATTTCGTCAGCGCATCGAAATGC TGCTTTCTGCACAGACCGTCGTGGGGCAAGTGTTGGTGATCCTTGTCTTTGTACTAAGCATCGGGTCTCT TGTGATCTATTTCATCAATTCAATGGATCCTGTTCGAAGGTGTTCTTCATATGAAGACAAAATTGTCCAT GTGGATTTGAGTTTCAACGCTTTCTTTAGCTTCTATTTTGGGTTGAGGTTTTGGGCAGCTGAAGACAAGA TCAAGTTCTGGTTGGAGATGAATTCAATTGTAGACATTTTTACCATCCCGCCAACCTTTATTTCTTATTA TTTGAAGAGTAATTGGCTAGGTTTGAGATTTCTAAGAGCTCTGCGGTTGCTCGAACTCCCTAAAATCTTA CAGATCCTACAAGTCATCAAGACCAGCAATTCAGTGAAGCTTTCCAAACTGTTGTCAATAGTTATCAGTA CCTGGTTCACGGCAGCAGGATTCCTTCACCTGGTGGAAAATTCTGGTGACCCCTGGCTCAACGGAAGAAA CTCACAGACTATGTCATACTTTGAGTCTATTTATCTGGTGACAGCAACAATGTCAACTGTTGGCTTTGGG GACGTGGTGGCCAAGACATCCCTAGGACGGATTTTCATTGTTTTCTTCACCCTTGGGAGTTTGATACTAT TTGCAAACTACATTCCAGAAATGGTGGAGCTCTTTTCTACCAGGAAGAAATACACCAAGCCCTACGAAGC AGTCAAAGGAAAAAAGTTCATCGTGGTCTGTGGAAACATCACAGTTGACAGTGTTACTGCTTTCCTGAGG AATTTTCTCCACTGGAAGTCCGGGGAAATCAATATTGAGATCGTATTCCTTGGAGAGACTCTCCCTTGCT TGGAACTGGAGACCTTACTGAAGTGCCACACATCCTGTACCAACTTCGTATGCGGCACCGCACTGAAGTT CGAGGATCTGAAGCGAGTTGCAGTGGAGAACTCGGAGGCGTGCCTGATTCTAGCCAACCATTTCTGTAGT GACTTACATGACGAAGACAACTCAAACATTATGAGGGTGCTCTCGATCAAGAACTATTATCCACAGACCA GAGTCATCATTCAGATACTTCAGTCTCAAAACAAGGTTTTCCTGTCAAAAATCCCCAACTGGGACTGGAG TGCTGGAGACAATATCCTCTGCTTTGCAGAGCTAAAGCTCGGATTTATCGCCCAAGGCTGCTTGGTGCCA GGGCTGTGCACCTTTCTCACGACTCTGTTCATTGAACAAAACCAAAAGGTTTTTCCTAAACATCCCTGGC AAAAACATTTCTTGAATGGCTTGAAGAACAAGATTCTGACACAGCGCCTCTCTAACGACTTCGTGGGGAT GACATTTCCCCAGGTCTCCCGGCTCTGCTTTGTGAAGCTAAATCTCATGCTGATCGCCATCCAACACAAG CCCTTCTTTCACAGTTGTTGCACTCTGATACTAAACCCATCATCCCAAGTGAGGCTGAATAAGGACACCT TAGGGTTCTTCATTGCGGACTCCTCCAAAGCCGTCAAAAGGGCTTTCTTTTACTGTTCCAACTGTCACAG CGATGTGTGCAATCCTGAGCTAATTGGAAAGTGTAACTGTAAAATCAAGAGCCGACAACAACTCATAGCA CCGACCATCATGGTGATGAAAAGCAGCTTGACCGATTTCACCACTTCTTCACACATCCACGCTTCTATGT CAACAGAAATTCACACTTGTTTTTCAAGAGAACAGCCTAGTTTGATCACCATTACAACCAACAGACCAAC GACAAACGACACAGTGGATGATACCGACATGCTGGACAGCAGTGGCATGTTTCACTGGTGCAGAGCAATG CCCTTGGACAAGGTGGTTCTGAAACGAAGTGAGAAGGCAAAACACGAGTTTCAGAACCACATTGTAGTAT GCGTGTTTGGAGATGCCCAATGTACCCTGGTGGGGCTTCGGAATTTCGTGATGCCCCTGAGAGCCAGCAA CTACACCCGGCAGGAGCTGAAGGACATTGTTTTTATTGGGTCTCTGGAGTACTTCCAGAGAGAATGGCGA TTTCTCCGAAACTTTCCCAAGATACACATTATGCCTGGATCTGCACTCTACATGGGAGATCTGATTGCAG TCAATGTAGAGCAGTGCTCTATGTGCGTCATCTTAGCCACACCCTACAAGGCACTGAGCAGCCAGATTCT GGTGGACACAGAGGCCATCATGGCCACCCTCAACATCCAGTCCCTGCGGATCACCAGTCCTACTCCAGGG TCTTCAAAGTCAGAAGTAAAGCCATCATCTGCCTTTGATAGTAAAGAAAGGAAGCAAAGATACAAACAGA TCCCCATTCTCACTGAACTGAAGAATCCCTCCAACATCCACTTTATTGAGCAGATGGGCGGACTGGATGG AATGCTCAAAGGGACTAGCTTGCATCTCAGCACTTCTTTCTCCACCGGTGCTGTCTTTTCAGACACCTTC TTGGATTCTCTCCTGGCCACGTCCTTCTACAATTACCATGTCGTGGAATTACTTCAGATGCTAGTGACTG GAGGCATAAGCTCTGAGATGGAACACTATTTGGTTAAGGAGAAGCCCTATAAGACAACTGACGACTATGA GGCAATCAAGTCTGGGAGGACGCGGTGTAAGCTGGGACTCCTCTCTTTAGACCAAACCGTTCTATCAGGC ATTAATCCAAGAAAAACCTTTGGACAGCTGTTCTGTGGCTCATTGGATAATTTCGGGATCCTATGTGTCG GCTTATACCGTATGATTGATGAAGAGGAACCCAGCCAAGAACACAAAAGGTTTGTGATCACCAGGCCATC CAATGAGTGCCACCTGCTGCCCTCAGATCTCGTGTTTTGTGCCATCCCTTTCAACACCACCTGTGGCAAA TCAGACAGCAGTCCTTCAATTCAGGCTCAAAACAACTCTACAAACGCGACGACGCCATTGGCCCAGGGGT CGAATTTCTTCGATTCGCACCATGCCGACGAGTCCCACGATCTTTACCCAGTCGACGACACGGGAGAGAG GTGGTCTCAGCACCACCACTCCCGAGTCTATCCTTTGGACACGTTAGATGCCAGTGATATTGTTCAAGAA AAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224899 representing NM_008432
Red=Cloning site Green=Tags(s) MSQTLLDSLNQKELTETSCTIEIQAAFILSSLATFFGGLIILFLFRIALKSSRSWKYVKGPRGLLELFSS RRIEANPLRKLYFHGVFRQRIEMLLSAQTVVGQVLVILVFVLSIGSLVIYFINSMDPVRRCSSYEDKIVH VDLSFNAFFSFYFGLRFWAAEDKIKFWLEMNSIVDIFTIPPTFISYYLKSNWLGLRFLRALRLLELPKIL QILQVIKTSNSVKLSKLLSIVISTWFTAAGFLHLVENSGDPWLNGRNSQTMSYFESIYLVTATMSTVGFG DVVAKTSLGRIFIVFFTLGSLILFANYIPEMVELFSTRKKYTKPYEAVKGKKFIVVCGNITVDSVTAFLR NFLHWKSGEINIEIVFLGETLPCLELETLLKCHTSCTNFVCGTALKFEDLKRVAVENSEACLILANHFCS DLHDEDNSNIMRVLSIKNYYPQTRVIIQILQSQNKVFLSKIPNWDWSAGDNILCFAELKLGFIAQGCLVP GLCTFLTTLFIEQNQKVFPKHPWQKHFLNGLKNKILTQRLSNDFVGMTFPQVSRLCFVKLNLMLIAIQHK PFFHSCCTLILNPSSQVRLNKDTLGFFIADSSKAVKRAFFYCSNCHSDVCNPELIGKCNCKIKSRQQLIA PTIMVMKSSLTDFTTSSHIHASMSTEIHTCFSREQPSLITITTNRPTTNDTVDDTDMLDSSGMFHWCRAM PLDKVVLKRSEKAKHEFQNHIVVCVFGDAQCTLVGLRNFVMPLRASNYTRQELKDIVFIGSLEYFQREWR FLRNFPKIHIMPGSALYMGDLIAVNVEQCSMCVILATPYKALSSQILVDTEAIMATLNIQSLRITSPTPG SSKSEVKPSSAFDSKERKQRYKQIPILTELKNPSNIHFIEQMGGLDGMLKGTSLHLSTSFSTGAVFSDTF LDSLLATSFYNYHVVELLQMLVTGGISSEMEHYLVKEKPYKTTDDYEAIKSGRTRCKLGLLSLDQTVLSG INPRKTFGQLFCGSLDNFGILCVGLYRMIDEEEPSQEHKRFVITRPSNECHLLPSDLVFCAIPFNTTCGK SDSSPSIQAQNNSTNATTPLAQGSNFFDSHHADESHDLYPVDDTGERWSQHHHSRVYPLDTLDASDIVQE K TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008432 |
| ORF Size | 3363 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008432.3, NP_032458.3 |
| RefSeq Size | 3548 bp |
| RefSeq ORF | 3366 bp |
| Locus ID | 16532 |
| UniProt ID | O54982 |
| MW | 126.9 kDa |
| Gene Summary | Testis-specific potassium channel activated by both intracellular pH and membrane voltage that mediates export of K(+). Represents the primary spermatozoan K(+) current. In contrast to KCNMA1/SLO1, it is not activated by Ca(2+) or Mg(2+). Critical for fertility. May play an important role in sperm osmoregulation required for the acquisition of normal morphology and motility when faced with osmotic challenges, such as those experienced after mixing with seminal fluid and entry into the vagina.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223642 | Kcnu1 (untagged) - Mouse potassium channel, subfamily U, member 1 (Kcnu1), (10ug) |
CNY 14,350.00 |
|
| MG224899 | Kcnu1 (tGFP-tagged) - Mouse potassium channel subfamily U member 1 (Kcnu1), (10ug) |
CNY 9,220.00 |
|
| MR224899L3 | Lenti ORF clone of Kcnu1 (Myc-DDK-tagged) - Mouse potassium channel, subfamily U, member 1 (Kcnu1) |
CNY 10,260.00 |
|
| MR224899L4 | Lenti ORF clone of Kcnu1 (mGFP-tagged) - Mouse potassium channel, subfamily U, member 1 (Kcnu1) |
CNY 10,260.00 |
