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Pawr (NM_054056) Mouse Tagged ORF Clone
CAT＃: MR225079
- TrueORF®
Pawr (Myc-DDK-tagged) - Mouse PRKC, apoptosis, WT1, regulator (Pawr)
ORF Plasmid: tGFP
"NM_054056" in other vectors (4)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310001G03Rik; Par-4; PAR4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225079 representing NM_054056
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCGGCGGCTACCGGAGCGGCGGCAGCACCACCACGGACTTCCTGGAGGAGTGGAAGGCGAAGC GCGAGAAGATGCGCGCCAAGCAGAACCCCGCGGGCCCGGGGTCGAGCGGCGGGGATCCAGCCGCCAAGTC CCCCGCGGGATCGCTGACCCCGACCGCGGTCGCGGGAACCTCGGAGCTCAACCACGGCCCCGCGGGCGCG GCCGCACCTGCCGCCCCCGCGCCCGGCGCCCTGAACTGCGCTCACGGCTCGTCCACGCTGCCCCGCGCGG CTCCCGGCTCCCGGCGGGCGGAGGACGAGTGCCCTAGCGCCGCTGCGGCCTCGGGAGCGCCTGGGTCCCG GGGCGATGAGGAGGAGCCGGATAGCGCCCGGGAGAAGGGCCGCAGCTCGGGACCCAGCGCCAGGAAAGGC AAGGGGCAGATCGAGAAGAGGAAGCTGCGGGAGAAGCGCCGCTCCACCGGCGTGGTCAACATCCCCGCGG CGGAGTGCTTAGATGAGTACGAAGATGATGAAGCAGGACAGAAGGAACGGAAGCGAGAGGATGCCATCAC CCAGCAAAACACCATCCAGAATGAAGCTGCGACCCTCCCAGATCCAGGCACATCCTACCTGCCCCAGGAC CCGTCGAGAACAGTTCCAGGCAGATACAAAAGCACAACCAGTGCCCCAGAAGATGAAATCTCAAATAGAT ATCCCCGAACAGACAGAAGTGGTTTCAGTAGACACAACAGAGATGCAAATGCGCCGGCTAGTTTCTCCTC AAGTAGCACCTTGGAAAAGAGAATTGAAGATCTTGAAAAGGAAGTTGTAAGAGAAAGGCAAGAAAACCTT CGACTTGTGAGGCTGATGCAAGATAAAGAAGAAATGATTGGGAAACTCAAGGAAGAGATCGATTTGTTAA ATAGAGACCTAGATGACATGGAAGATGAGAACGAGCAACTAAAACAGGAAAATAAAACTCTTTTGAAGGT TGTTGGGCAGCTGACAAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225079 representing NM_054056
Red=Cloning site Green=Tags(s) MATGGYRSGGSTTTDFLEEWKAKREKMRAKQNPAGPGSSGGDPAAKSPAGSLTPTAVAGTSELNHGPAGA AAPAAPAPGALNCAHGSSTLPRAAPGSRRAEDECPSAAAASGAPGSRGDEEEPDSAREKGRSSGPSARKG KGQIEKRKLREKRRSTGVVNIPAAECLDEYEDDEAGQKERKREDAITQQNTIQNEAATLPDPGTSYLPQD PSRTVPGRYKSTTSAPEDEISNRYPRTDRSGFSRHNRDANAPASFSSSSTLEKRIEDLEKEVVRERQENL RLVRLMQDKEEMIGKLKEEIDLLNRDLDDMEDENEQLKQENKTLLKVVGQLTR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_054056 |
| ORF Size | 999 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_054056.2, NP_473397.1 |
| RefSeq Size | 1828 bp |
| RefSeq ORF | 1002 bp |
| Locus ID | 114774 |
| UniProt ID | Q925B0 |
| MW | 36.4 kDa |
| Gene Summary | Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212285 | Pawr (untagged) - Mouse PRKC, apoptosis, WT1, regulator (Pawr), (10ug) |
CNY 3,990.00 |
|
| MG225079 | Pawr (tGFP-tagged) - Mouse PRKC apoptosis WT1 regulator (Pawr), (10ug) |
CNY 2,850.00 |
|
| MR225079L3 | Lenti ORF clone of Pawr (Myc-DDK-tagged) - Mouse PRKC, apoptosis, WT1, regulator (Pawr) |
CNY 4,750.00 |
|
| MR225079L4 | Lenti ORF clone of Pawr (mGFP-tagged) - Mouse PRKC, apoptosis, WT1, regulator (Pawr) |
CNY 4,750.00 |
