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Rasgrp4 (NM_145149) Mouse Tagged ORF Clone
CAT＃: MR225103
- TrueORF®
Rasgrp4 (Myc-DDK-tagged) - Mouse RAS guanyl releasing protein 4 (Rasgrp4), transcript variant 2
ORF Plasmid: tGFP
"NM_145149" in other vectors (4)
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CNY 6,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225103 representing NM_145149
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCGGAAAGACATCAAAAGGAAGTCTCATCAGGAATGCTCTGGGAAGGCAGGAGGGCGGGGTCGGT CACGTCAGGCCCGCCGCCACAAGACGTGCCCCACCCCCCGGGAAATCAGCAAGGTCATGGCGTCCATGAA TCTGGGAGTGCTGAGTGAGAGCAGCTGCAGCGAAGATGAGCTATTGGAGGAATGTATCCGCTGCTTTGAC TCGGCTGGCAGCCTGCGCCGAGGGGACCACATTCTCAAGATGGTGCTCACGATGCACAGCTGGGTGCTGC CATCCTCAGAGCTCGCTGCCCGTCTGCTGACTTCGTATCAGAAGGCTGCCAAAGATGCCCAAGAGCTAAG ACAGCTACAGATCTGTTACTTGGTCAGGTACTGGCTGACCCATCACCATGAGGCAGTGCACCAGGAACCC CAGCTGGAAGCAGTCATAAGCCGGTTTTGGACCACTGTCGCTCAGGAGGGCAACATGGCCCAAAGGAGCC TGGGAGATGCCTCCAGCCTGCTAAGTCCTGGAGGGCCCGGTCCCCCACCTCCCATGAGCAGCCCAGGCCT GGGCAAGAAGCGGAAAGTGTCATTGCTATTCGATCACCTGGAGACAGAGGAGCTGGCACAACACCTCACT TACCTGGAGTTCCGGTCTTTCCAGGCTATCACACCCCAAGACCTGCGGGGCTATGTTTTGCAGGGTTCAG TGAGAGGTTGTCCAGCTCTGGAAGGTTCTGTGGGTCTGAGCAACAGTGTGTCCCGCTGGGTGCAGGTCAT GGTGCTGAGTCGTCCTGGGCCTGCACAACGTGCCCAGGTCCTGGACAAATTCATTCGTGTGGCACAGAGG CTTCACCAGCTGCAGAATTTCAACACACTGATGGCGGTCACGGGGGGTCTGTGTCATAGCGCCATCTCCA GACTCAAGGACTCCCACGTTCATCTGAGCCCTGACAGCACAAAGGCCCTGCTGGAGCTGACAGAGCTCCT CTCATCCCACAACAACTATGCCCACTACCGCCGCACCTGGGCCGGCTGTACTGGCTTCCGGCTGCCAGTA CTGGGTGTGCACCTCAAGGACCTGGTATCTCTATATGAGGCTCATCCAGACAGATTGCCAGATGGCCGCC TGCACCTACCCAAGCTGAATAGCCTCTATCTACGGCTGCAGGAGCTGATGGCGCTCCAGGGACAGCATCC TCCCTGCAGTGCCAATGAGGACCTGCTGCACCTGCTGACGCTCTCCCTGGATCTCTTCTACACGGAAGAT GAGATCTACGAGCTTTCTTATGCCCGGGAACCACGCTGTCCCAAGAGTCTGCCACCGTCCCCCTTCAAAG CACCAGTGGTGGTAGAGTGGGCCCAGGGTGTGACACCAAAGCCAGACAGCGTGACTCTGGGTCAGCATGT GGAACAGCTGGTGGAGTCCGTGTTCAAGAACTACGACCCAGAAGGCCGTGGCTCCATCTCTCTGGAGGAC TTTGAGCGGCTGTCAGGCAACTTCCCGTTCGCCTGCCATGGGCTTCACCCACCTCCCCGCCATGGGAGTG GCTCCTTCAGCAGAGAGGAGCTGACCAAGTACCTGCTCCATGCCAGTGCCATCTGCTCCAAGCTGGGCCT GGCCTTCCTGCACGCCTTCCAGGAGGTCACCTTCCGAAAGCCCACGTTCTGTCACAGCTGCAGCGGCTTC CTCTGGGGGGTCACCAAGCAAGGCTATCGCTGTCGGGACTGTGGGCTGTGTTGTCACAGACACTGCAGGG ATCAAGTGAGAGTGGAGTGTAAGAAGAGGCCAGAGACTAAGGGTGACCCGGGCCCCCCAGGTGCACCTGT GCCAGCCACATCACTTCCTCCTGCCAACTGTGGCTCAGAGGAAAGTCTCTCCTATACACTCTCCCCGGAT CCGGAGTCTGGTTGCCACCTTCGCCATGCTTGGACCCAGACGGAATCCTCACACTCTTCCTGGGAGCCAG AGGTGGTGCCCTGCCCAGCACGGGTCTTACCATCCAGAGCTTCCTCGAAGCCCAGCGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225103 representing NM_145149
Red=Cloning site Green=Tags(s) MNRKDIKRKSHQECSGKAGGRGRSRQARRHKTCPTPREISKVMASMNLGVLSESSCSEDELLEECIRCFD SAGSLRRGDHILKMVLTMHSWVLPSSELAARLLTSYQKAAKDAQELRQLQICYLVRYWLTHHHEAVHQEP QLEAVISRFWTTVAQEGNMAQRSLGDASSLLSPGGPGPPPPMSSPGLGKKRKVSLLFDHLETEELAQHLT YLEFRSFQAITPQDLRGYVLQGSVRGCPALEGSVGLSNSVSRWVQVMVLSRPGPAQRAQVLDKFIRVAQR LHQLQNFNTLMAVTGGLCHSAISRLKDSHVHLSPDSTKALLELTELLSSHNNYAHYRRTWAGCTGFRLPV LGVHLKDLVSLYEAHPDRLPDGRLHLPKLNSLYLRLQELMALQGQHPPCSANEDLLHLLTLSLDLFYTED EIYELSYAREPRCPKSLPPSPFKAPVVVEWAQGVTPKPDSVTLGQHVEQLVESVFKNYDPEGRGSISLED FERLSGNFPFACHGLHPPPRHGSGSFSREELTKYLLHASAICSKLGLAFLHAFQEVTFRKPTFCHSCSGF LWGVTKQGYRCRDCGLCCHRHCRDQVRVECKKRPETKGDPGPPGAPVPATSLPPANCGSEESLSYTLSPD PESGCHLRHAWTQTESSHSSWEPEVVPCPARVLPSRASSKPSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145149 |
| ORF Size | 2019 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145149.4, NP_660131.2 |
| RefSeq Size | 4621 bp |
| RefSeq ORF | 2022 bp |
| Locus ID | 233046 |
| UniProt ID | Q8BTM9 |
| MW | 75.4 kDa |
| Gene Summary | Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220375 | Rasgrp4 (untagged) - Mouse RAS guanyl releasing protein 4 (Rasgrp4), transcript variant 2, (10ug) |
CNY 8,650.00 |
|
| MG225103 | Rasgrp4 (tGFP-tagged) - Mouse RAS guanyl releasing protein 4 (Rasgrp4) transcript variant 2, (10ug) |
CNY 7,410.00 |
|
| MR225103L3 | Lenti ORF clone of Rasgrp4 (Myc-DDK-tagged) - Mouse RAS guanyl releasing protein 4 (Rasgrp4), transcript variant 2 |
CNY 8,650.00 |
|
| MR225103L4 | Lenti ORF clone of Rasgrp4 (mGFP-tagged) - Mouse RAS guanyl releasing protein 4 (Rasgrp4), transcript variant 2 |
CNY 8,650.00 |
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