Ddb2 (NM_028119) Mouse Tagged ORF Clone
CAT#: MR225146
- TrueORF®
Ddb2 (Myc-DDK-tagged) - Mouse damage specific DNA binding protein 2 (Ddb2)
ORF Plasmid: tGFP
"NM_028119" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2610043A19Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225146 representing NM_028119
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCCAAGAAATGCCCAGAAACCCAGAAGTCCCCCGACGTTGCGGTGCTCCTCAGGAGCAAAAGTC GCAGAGGTCCTCAGGAGCTGGAGCCCGAAGCCAAGAAGCTGCGTGTGCAGGGTCCCGTTTCTAGCAGGAC ATGTGAGTCGTGCTGCCTTCTGGCAGAGTTGTCCAGCCTGCAGATCCCTTCACGGAGTAGCAGCATTGTC AGGGATCTTTACCAGCATAAGTTGGGCAAAGCCACCTGGTCATCACTACAGCAGGGTCTGCAGAAGTCCT TTTTGCACTCTCTAGCTTCTTACCAGGTATTCCGAAAAGCTGCCCCCTTTGACAGGAGGACTACGTCCTT GGCATGGCACCCGACTCATCCCAGTACCCTGGCTGTGGGCTCCAAAGGGGGAGATATTATGATCTGGAAC TTTGGCATCAAGGACAAACCTATCTTCCTTAAAGGGATTGGAGCTGGAGGAAGCATCACTGGGCTGAAGT TTAACCATCTCAATACCAACCAGTTTTTTGCCTCCTCAATGGAGGGAACAACCAGGCTGCAGGATTTTAA AGGCAACATTCTCAGAGTTTATACCAGCTCAAACTCTTGCAAGGTCTGGTTTTGCAGCCTTGATGTTTCT GCCAAGAGCAGAGTGGTGGTTACAGGAGACAATATGGGACATGTGATCCTGTTGAGCACAGATGGCAAGG AGCTTTGGAACCTCCGAATGCACAAGAAGAAAGTAGCCCACGTGGCCCTGAATCCCTGCTGTGATTGGCT TCTGGCCACAGCCTCCATAGATCAAACAGTGAAGATTTGGGACCTGCGCCAAATTAAAGGGAAAGACAGC TTCCTCTACTCACTGCCTCACAGGCATCCTGTCAATGCAGCTTGTTTTAGCCCAGATGGAGCTCGGCTCC TGACTACTGACCAGAACAATGAGATTCGGGTTTACTCTGCCTCCCAGTGGGATAGCCCCCTGAATCTGAT CTCCCACCCTCACCGCCATTTTCAGCACCTCACACCCATCAAGGCGACCTGGCATTCACGGCACAACCTC ATTGTTGTGGGCCGATACCCAGATCCTAATCTTAAAAGTTGTGTTCCCTATGAACTAAGGACAATAGATG TGTTTGATGGAAGCTCAGGGAAGATGATGTGTCAGCTCTATGATCCAGGATACTCCGGTATTACTTCGCT CAATGAGTTCAATCCTATGGGAGACACACTGGCCTCTACTATGGGTTATCATATTCTCATTTGGAGCCAA GAGGAAGATGGGTCACAGAAAGATCATGAAAGACTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225146 representing NM_028119
Red=Cloning site Green=Tags(s) MAPKKCPETQKSPDVAVLLRSKSRRGPQELEPEAKKLRVQGPVSSRTCESCCLLAELSSLQIPSRSSSIV RDLYQHKLGKATWSSLQQGLQKSFLHSLASYQVFRKAAPFDRRTTSLAWHPTHPSTLAVGSKGGDIMIWN FGIKDKPIFLKGIGAGGSITGLKFNHLNTNQFFASSMEGTTRLQDFKGNILRVYTSSNSCKVWFCSLDVS AKSRVVVTGDNMGHVILLSTDGKELWNLRMHKKKVAHVALNPCCDWLLATASIDQTVKIWDLRQIKGKDS FLYSLPHRHPVNAACFSPDGARLLTTDQNNEIRVYSASQWDSPLNLISHPHRHFQHLTPIKATWHSRHNL IVVGRYPDPNLKSCVPYELRTIDVFDGSSGKMMCQLYDPGYSGITSLNEFNPMGDTLASTMGYHILIWSQ EEDGSQKDHERL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_028119 |
ORF Size | 1296 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_028119.5, NP_082395.2 |
RefSeq Size | 1929 bp |
RefSeq ORF | 1299 bp |
Locus ID | 107986 |
UniProt ID | Q99J79 |
MW | 48.8 kDa |
Gene Summary | Required for DNA repair. Binds to DDB1 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212159 | Ddb2 (untagged) - Mouse damage specific DNA binding protein 2 (Ddb2), (10ug) |
CNY 3,990.00 |
|
MG225146 | Ddb2 (tGFP-tagged) - Mouse damage specific DNA binding protein 2 (Ddb2), (10ug) |
CNY 3,710.00 |
|
MR225146L3 | Lenti ORF clone of Ddb2 (Myc-DDK-tagged) - Mouse damage specific DNA binding protein 2 (Ddb2) |
CNY 5,230.00 |
|
MR225146L4 | Lenti ORF clone of Ddb2 (mGFP-tagged) - Mouse damage specific DNA binding protein 2 (Ddb2) |
CNY 5,230.00 |