United States
Germany
Japan
United Kingdom
China
Sox15 (NM_009235) Mouse Tagged ORF Clone
CAT＃: MR225149
- TrueORF®
Sox15 (Myc-DDK-tagged) - Mouse SRY-box containing gene 15 (Sox15)
ORF Plasmid: tGFP
"NM_009235" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225149 representing NM_009235
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGACCAGCTCCTCACAAGCAGAGACTTGGAGCCTGCATCCTCGGGCTTCCACGGCCTCTTTGC CTTTAGGACCCCAGGAGCAGGAGGCCGGCGGGAGCCCTGGAGCGTCTGGGGGACTTCCGCTGGAGAAGGT GAAGCGGCCCATGAACGCCTTCATGGTGTGGAGCTCTGTTCAGCGCCGCCAGATGGCGCAGCAGAACCCC AAGATGCACAACTCTGAGATCTCCAAGCGCTTGGGCGCTCAGTGGAAGCTGCTGGGCGATGAAGAGAAGC GACCCTTCGTGGAGGAGGCTAAGCGTCTTCGTGCCCGCCACCTCCGCGACTATCCCGACTACAAGTACCG ACCCCGGCGTAAGAGCAAAAACTCGAGCACCGGGTCTGTCCCCTTTAGCCAAGAAGGAGGTGGCCTGGCA TGTGGTGGCTCACACTGGGGGCCAGGGTACACAACTACCCAAGGGAGCAGAGGCTTTGGGTACCAGCCCC CCAACTATTCGACAGCCTACCTGCCTGGCAGTTACACCTCTTCCCACTGCAGACCGGAGGCCCCCTTACC ATGCACCTTCCCTCAGAGTGATCCCAGGCTCCAGGGGGAGCTAAGACCCTCTTTCTCCCCTTACCTATCC CCAGACTCTTCCACTCCATATAATACTTCCCTTGCTGGAGCCCCCATGCCAGTAACCCACCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225149 representing NM_009235
Red=Cloning site Green=Tags(s) MALTSSSQAETWSLHPRASTASLPLGPQEQEAGGSPGASGGLPLEKVKRPMNAFMVWSSVQRRQMAQQNP KMHNSEISKRLGAQWKLLGDEEKRPFVEEAKRLRARHLRDYPDYKYRPRRKSKNSSTGSVPFSQEGGGLA CGGSHWGPGYTTTQGSRGFGYQPPNYSTAYLPGSYTSSHCRPEAPLPCTFPQSDPRLQGELRPSFSPYLS PDSSTPYNTSLAGAPMPVTHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009235 |
| ORF Size | 693 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009235.2, NP_033261.1 |
| RefSeq Size | 1156 bp |
| RefSeq ORF | 696 bp |
| Locus ID | 20670 |
| UniProt ID | P43267 |
| MW | 25.3 kDa |
| Gene Summary | Transcription factor that binds to DNA at the 5'-AACAATG-3' consensus sequence (PubMed:10821863, PubMed:15863505, PubMed:16759287, PubMed:17363903). Acts as a transcriptional activator and repressor (PubMed:10821863, PubMed:15863505, PubMed:16759287). Binds synergistically with POU5F1 (OCT3/4) to gene promoters (PubMed:15863505). Binds to the FOXK1 promoter and recruits FHL3, resulting in transcriptional activation of FOXK1 which leads to myoblast proliferation (PubMed:17363903). Acts as an inhibitor of myoblast differentiation via transcriptional repression which leads to down-regulation of the muscle-specific genes MYOD and MYOG (PubMed:10821863). Involved in trophoblast giant cell differentiation via enhancement of HAND1 transcriptional activity (PubMed:16759287). Regulates transcription of HRC via binding to it proximal enhancer region (PubMed:15863505). Involved in skeletal muscle regeneration (PubMed:15367664, PubMed:17363903). Also plays a role in the development of myogenic precursor cells (PubMed:15367664).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209432 | Sox15 (untagged) - Mouse SRY-box containing gene 15 (Sox15), (10ug) |
CNY 3,990.00 |
|
| MG225149 | Sox15 (tGFP-tagged) - Mouse SRY-box containing gene 15 (Sox15), (10ug) |
CNY 2,850.00 |
|
| MR225149L3 | Lenti ORF clone of Sox15 (Myc-DDK-tagged) - Mouse SRY-box containing gene 15 (Sox15) |
CNY 4,750.00 |
|
| MR225149L4 | Lenti ORF clone of Sox15 (mGFP-tagged) - Mouse SRY-box containing gene 15 (Sox15) |
CNY 4,750.00 |
