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Trpc7 (NM_012035) Mouse Tagged ORF Clone
CAT＃: MR225262
- TrueORF®
Trpc7 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 7 (Trpc7)
ORF Plasmid: tGFP
"NM_012035" in other vectors (6)
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CNY 10,088.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TR; TRP7; Trrp8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225262 representing NM_012035
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGGGAGCAACACCTTCAAAAACATGCAGCGCCGGCACACCACCTTGAGGGAGAAGGGGCGCCGCC AGGCCATCCGGGGTCCTGCCTACATGTTCAACGAGAAGGGCACGAGCCTGACCCCTGAGGAGGAGCGCTT CCTGGACTCGGCTGAGTATGGCAACATACCAGTGGTCAGAAAAATGCTGGAGGAATCCAAGACCCTCAAT TTCAACTGCGTGGACTACATGGGGCAGAACGCGCTGCAGCTGGCCGTGGGCAACGAGCACTTGGAGGTCA CTGAACTGTTGCTGAAGAAAGAGAACTTGGCGCGGGTGGGCGACGCGCTGCTGCTGGCCATCAGCAAGGG CTATGTGCGCATTGTGGAGGCCATCCTCAGCCATCCGGCCTTCGCGCAGGGCCAGCGCCTGACGCTCAGC CCGCTGGAGCAGGAGCTGCGGGATGATGACTTCTATGCCTACGACGAGGATGGCACGCGCTTCTCCCACG ACATCACACCCATCATCCTGGCGGCCCACTGCCAGGAGTATGAGATTGTGCATATCTTGCTGCTCAAGGG TGCGCGCATTGAGCGGCCCCATGACTACTTCTGCAAGTGCAATGAGTGCACGGAGAAGCAGCGCAAGGAC TCCTTCAGTCACTCGCGCTCCCGGATGAATGCCTACAAAGGACTGGCCAGTGCCGCCTACCTGTCCCTAT CCAGTGAAGACCCTGTCCTCACTGCGCTGGAGCTAAGCAACGAGTTAGCCCGACTGGCCAACATTGAGAC TGAATTTAAGAATGATTACAGGAAGTTATCTATGCAATGCAAGGATTTTGTGGTGGGCGTGCTGGACCTG TGCCGAGACACAGAAGAGGTGGAAGCAATTTTAAATGGCGATGTGAACTTGCAAGTCTGGTCCGACCACC ACCGTCCAAGTCTGAGCCGAATCAAACTCGCCATTAAGTACGAAGTCAAGAAGTTCGTTGCTCACCCTAA CTGTCAGCAGCAATTGCTTACCATGTGGTATGAAAATCTCTCAGGCTTACGGCAACAGTCTATCGCTGTG AAATTCCTGGCTGTCTTTGGAGTCTCCATAGGCCTCCCTTTTCTCGCCATAGCCTATTGGATTGCTCCGT GCAGCAAGCTAGGACAAACCCTGAGGAGCCCTTTCATGAAGTTTGTGGCCCATGCAGTGTCTTTTACCAT CTTCTTGGGACTGCTAGTTGTGAACGCCTCCGACCGGTTTGAGGGCGTTAAGACTCTGCCCAATGAGACC TTCACAGACTACCCCAAACAGATCTTCAGAGTGAAAACCACACAGTTCTCCTGGACGGAGATGCTCATCA TGAAGTGGGTCTTAGGAATGATCTGGTCCGAATGCAAGGAGATCTGGGAGGAGGGGCCGCGGGAGTACGT GCTACACTTGTGGAACCTTCTGGATTTTGGCATGCTGTCCATCTTCGTGGCCTCCTTCACTGCGAGGTTC ATGGCTTTCCTCAAGGCCAGCGAGGCCCAGCTGTACGTGGACCAGTACGTGCAGGATGTAACGCTGCACA ACGTCTCACTTCCACCGGAAGTGGCCTACTTCACCTACGCCAGGGATAAGTGGTGGCCTTCAGACCCCCA GATCATCTCGGAAGGGCTGTACGCCATTGCGGTTGTCCTCAGCTTCTCCCGGATCGCCTACATCCTGCCA GCCAATGAGAGCTTCGGACCTTTGCAGATCTCCCTGGGAAGAACTGTGAAAGACATCTTCAAGTTCATGG TCATTTTTATCATGGTATTTGTGGCCTTTATGATCGGAATGTTCAACCTGTACTCCTACTACCGAGGTGC AAAGTACAACCCAGCGTTTACCACAGTTGAAGAAAGCTTTAAAACCTTGTTTTGGTCCATATTTGGCTTG TCTGAGGTCATCTCGGTGGTGCTGAAATACGACCACAAGTTCATCGAGAATATTGGCTACGTGCTGTATG GGGTTTATAATGTCACCATGGTGGTTGTACTACTCAACATGCTGATAGCCATGATCAACAACTCCTATCA GGAAATCGAGGAAGACGCGGACGTGGAGTGGAAATTTGCTCGAGCGAAGCTCTGGCTTTCTTACTTTGAT GAAGGAAGAACTCTCCCTGCTCCCTTTAACCTGGTGCCGAGCCCTAAATCCTTTTATTATCTCATAATGA GAATCAAGATGTGCCTCATAGAGCTCTGCCAATCTAAGGCCAAACGCTGTGAAAACGACCTGGAAATGGG CATGCTGAACTCCAAGTTCAGGAAGACTCGCTACCAGGCTGGCATGAGGAATTCTGAAAACCTGACAGCC AATAGCACCTTCAGCAAGCCCACCAGATACCAGAAGATCATGAAGCGGCTCATAAAGAGATACGTGCTGA AGGCCCAGGTGGACAGAGAGAACGATGAAGTCAATGAAGGTGAACTCAAGGAGATCAAACAAGACATCTC AAGCCTTCGCTATGAGCTCCTGGAGGAGAAGTCTCAGGCTACAGGAGAGCTGGCAGACCTGATCCAGCAG CTCAGCGAGAAGTTTGGGAAGAATCTGAACAAAGACCACCTGCGGGTGAACCAGGGCAAGGACATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225262 representing NM_012035
Red=Cloning site Green=Tags(s) MLGSNTFKNMQRRHTTLREKGRRQAIRGPAYMFNEKGTSLTPEEERFLDSAEYGNIPVVRKMLEESKTLN FNCVDYMGQNALQLAVGNEHLEVTELLLKKENLARVGDALLLAISKGYVRIVEAILSHPAFAQGQRLTLS PLEQELRDDDFYAYDEDGTRFSHDITPIILAAHCQEYEIVHILLLKGARIERPHDYFCKCNECTEKQRKD SFSHSRSRMNAYKGLASAAYLSLSSEDPVLTALELSNELARLANIETEFKNDYRKLSMQCKDFVVGVLDL CRDTEEVEAILNGDVNLQVWSDHHRPSLSRIKLAIKYEVKKFVAHPNCQQQLLTMWYENLSGLRQQSIAV KFLAVFGVSIGLPFLAIAYWIAPCSKLGQTLRSPFMKFVAHAVSFTIFLGLLVVNASDRFEGVKTLPNET FTDYPKQIFRVKTTQFSWTEMLIMKWVLGMIWSECKEIWEEGPREYVLHLWNLLDFGMLSIFVASFTARF MAFLKASEAQLYVDQYVQDVTLHNVSLPPEVAYFTYARDKWWPSDPQIISEGLYAIAVVLSFSRIAYILP ANESFGPLQISLGRTVKDIFKFMVIFIMVFVAFMIGMFNLYSYYRGAKYNPAFTTVEESFKTLFWSIFGL SEVISVVLKYDHKFIENIGYVLYGVYNVTMVVVLLNMLIAMINNSYQEIEEDADVEWKFARAKLWLSYFD EGRTLPAPFNLVPSPKSFYYLIMRIKMCLIELCQSKAKRCENDLEMGMLNSKFRKTRYQAGMRNSENLTA NSTFSKPTRYQKIMKRLIKRYVLKAQVDRENDEVNEGELKEIKQDISSLRYELLEEKSQATGELADLIQQ LSEKFGKNLNKDHLRVNQGKDI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012035 |
| ORF Size | 2583 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012035.3, NP_036165.1 |
| RefSeq Size | 3550 bp |
| RefSeq ORF | 2589 bp |
| Locus ID | 26946 |
| UniProt ID | Q9WVC5 |
| MW | 99.5 kDa |
| Gene Summary | The protein encoded by this gene is a member of the transient receptor potential channel family of proteins, which form six-transmembrane cation-permeable channels that are calcium permeant. Knock out mice are viable but display a reduction in the gamma wave activity that precedes seizure induction in response to a muscrarinic agonist, suggesting a functional role for this protein in initiation of seizures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222261 | Trpc7 (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 7 (Trpc7), (10ug) |
CNY 11,120.00 |
|
| MG225262 | Trpc7 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily C member 7 (Trpc7), (10ug) |
CNY 7,890.00 |
|
| MR225262L1 | Lenti ORF clone of Trpc7 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 7 (Trpc7) |
CNY 12,488.00 |
|
| MR225262L2 | Lenti ORF clone of Trpc7 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 7 (Trpc7) |
CNY 9,030.00 |
|
| MR225262L3 | Lenti ORF clone of Trpc7 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 7 (Trpc7) |
CNY 9,030.00 |
|
| MR225262L4 | Lenti ORF clone of Trpc7 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 7 (Trpc7) |
CNY 9,030.00 |
