Pikfyve (NM_011086) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5230400C17Rik; p235; Pip5k; Pip5k3; Pipk5k3; PipkIII
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR225815 representing NM_011086 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACAGATGACAAGAGTTCCCCGACACTGGACTCTGCTAATGATTTGCCTCGCTCTCCTGCCAGTC CTTCTCACCTCACTCACTTTAAACCCTTGACTCCTGACCAGGATGAGCCCCCCTTCAAGTCAGCATATAG TTCTTTTGTAAACCTTTTTCGTTTTAACAAAGAGCGAGGAGAAGGGGGCCAAGGAGAGCAGCAGTCTCCG AGTTCAAGTTGGGCCAGCCCTCAGATCCCTTCAAGAACACAGTCTGTGAGGTCGCCTGTACCTTATAAAA AACAGCTTAATGAGGAGCTCCACCGGCGCTCTTCAGTGTTAGAGAACACTTTGCCACATCCTCAGGAGAG CACAGACTCCAGAAGGAAAGCAGAACCAGCCTGTGGAGGTCATGACCCACGTACAGCTGTTCAGCTTCGA AGCCTCAGCACAGTATTGAAACGCCTCAAAGAAATCATGGAAGGAAAAAGCCAGGACAGTGACCTGAAGC AATATTGGATGCCAGATAGCCAGTGTAAAGAGTGCTATGACTGCAGTGAAAAGTTTACAACATTTAGGCG CAGACACCATTGCAGACTGTGTGGGCAGATTTTCTGCAGTCGTTGTTGTAATCAAGAAATCCCTGGAAAA TTTATGGGCTATACAGGAGACCTCCGAGCATGCACCTACTGTAGAAAAATAGCCTTAAGTTATGCTCATT CTACAGACAGTAATTCCATTGGGGAAGACTTGAATGCTCTTTCAGATTCAACTTGCTCTGTGTCTATACT TGATCCAAGCGAACCTCGGACACCAGTTGGGAGTAGAAAAGCCAGTCGTAACATATTCTTAGAGGATGAT TTAGCCTGGCAAAGCTTGATTCATCCGGATTCCTCAAATAGTGCTCTTTCAACAAGACTCGTATCTGTTC AAGAGGATGCTGGGAAGTCTCCTGCTCGAAACAGATCAGCCAGCATTACTAATCTGTCACTGGATCGGTC TGGTTCTCCTATGGTTCCTTCATATGAGACATCTGTCAGTCCCCAGGCTAACCGAAACTACATTAGGACA GAGACGACTGAGGATGAACGCAAAATTCTTCTGGACAGTGCTCAGTTAAAGGATCTGTGGAAGAAAATCT GCCATCACACCAGTGGGATGGAATTTCAAGATCACCGTTACTGGTTGAGAACACATCCCAACTGCATTGT AGGGAAGGAATTAGTCAACTGGCTAATCAGAAATGGACACATCGCTACAAGGGCACAAGCTATAGCAATT GGACAAGCAATGGTTGATGGACGTTGGTTGGATTGTGTTAGTCATCATGATCAGCTTTTCAGGGACGAAT ATGCGTTGTATAGACCACTTCAGAGTACAGAATTTTCTGAGACACCTTCTCCAGACAGTGACTCTGTGAA CTCTGTGGAAGGACACTCCGAGCCATCCTGGTTTAAAGACATAAAATTTGATGACAGTGACACAGAACAG ATTGCTGAAGAAGGTGACGATAATTTGGCTAAGTATTTGGTTTCTGACACTGGAGGACAGCAGCTCTCAA TAAGTGATGCCTTCATCAAAGAGTCCTTATTTAATCGACGAGTAGAGGAAAAATCCAAAGAGCTGCCTTT TACCCCTTTGGGCTGGCATCATAACAACCTGGAACTCTTGCGAGAGGAGAATGAGGAGAAGCAAGCCATG GAAAGGCTGCTTTCAGCTAATCATAACCACATGATGGCCCTACTCCAGCAGTTGCTTCAAAACGAGTCAT TGTCATCGTCTTGGAGGGACATCATTGTGTCACTAGTCTGCCAGGTTGTTCAGACAGTCCGACCTGATGT CAAGCACCAGGATGATGACATGGATATCCGTCAGTTTGTCCATATCAAGAAGATCCCAGGTGGAAAGAAA TTTGACTCTGTGGTTGTCAATGGCTTTGTTTGTACCAAGAACATTGCACATAAAAAGATGAATTCCTGTA TTAAAAACCCCAAAATCCTTCTGTTGAAGTGTTCTATTGAGTATCTCTATAGAGAAGAAACTAAGTTTAC CTGCATTGATCCTATTGTGCTTCAGGAAAGGGAATTCTTGAAGAATTATGTTCAACGAATAGTTGATGTT CGACCCACATTGGTTCTTGTTGAGAAAACAGTGTCTCGGATTGCTCAGGACATGTTACTGGAACATGGCA TTACTCTGGTCATTAATGTAAAGTCACAAGTTTTAGAAAGAATCAGTCGAATGACCCAAGGTGATTTAGT GGTGTCCATGGACCAGCTGCTCACCAAACCCCACTTGGGCACTTGCCACAAATTTTATATGCAGATATTT CAGCTGCCTAATGAACAAACCAAAACACTGATGTTTTTTGAAGGCTGTCCACAGCATCTAGGCTGCACAA TCAAGCTCAGAGGAGGCTCTGATTATGAGCTGGCTCGAGTTAAGGAGATCCTAATATTTATGATCTGTGT AGCTTATCATTCTCAGCTAGAAATCTCTTTTCTCATGGATGAGTTCGCTATGCCTCCAACATTAATGCAA AGCCCTTCATTCCATCTTCTGACGGAGGGACGAGGTGAAGAGGGAGCCTCTCAGGAGCAGGTCAGTGGCA GCTCCCTTCCTCAGGATCCTGAGTGCCCTCGTGAGGCCCTGTCTTCTGAGGATAGCACTTTGTTGGAATC AAGGACTGTGCTAGAGAAGGGTGAACTAGACAATAAAAGTATTCCACAAGCTGTTGCCTCTTTGAAGCAT CAAGATTATACCACCCCCACTTGCCCAGCAGGTATTCCCTGTGCTCTTTTTGCATTGGTACCAGAGTCAT TGTTGCCTCTCCATATGGATCAACAGGATGCCGTAGGAAATGAACAGCCAGAGACTTCACAGCAAACGGA TGAGCAACAGGATCCCAAAAGCCAGATGAAAGCTTTTAGAGACCCTTTACAGGATGACACTGGAATGTAC GTTACTGAGGAAGTCACCTCCTCTGAAGATCAACGAAAGACTTATGCCTTGACATTTAAACAGGAGTTAA AAGATGTAATCCTCTGTATCTCTCCAGTTATTACATTCCGTGAACCTTTCCTTTTAACTGAAAAGGGGAT GAGATGCTCAACTCGAGATTATTTTCCAGAGCAGATTTACTGGTCTCCTCTTCTCAACAAAGAGGTGAAG GAAATGGAGAGCAGGAGGAAGAAACAGCTGCTCAGGGATCTCTCTGGACTTCAGGGCATGAATGGCAGTG TTCAGGCCAAGTCTATTCAAGTCTTACCCTCACATGAGCTAGTGAGCACCAGGATTGCTGAACATCTGGG TGACAGCCAGACCTTGGGTAGAATGCTAGCTGATTATCGAGCTAGAGGAGGAAGAATTCAGTCAAAACAT TTGGACCCCTTTGTCCATTCAAAAGATGCATCATGTACTTCAGGTGGCAAATCAGGAAACAAAACTGAGA GTGATGAAGAGAGGGGATTGATTCCAAGTGATGTAATATGGCCAACAAAGGTGGACTGTCTGAACCCTGC TAACCACCAGAGGCTCTGTGTGCTCTTCAGCAGCTCTTCTGCCCAGTCCAGCAATGCTCCCAGTGCTTGT GTCAGTCCTTGGATTGTAACAATGGAGTTTTATGGAAAGAATGACCTTACACTGGGGATATTTTTAGAAA GATACTGTTTCAGGTCTTCTTACCAGTGTCCGAGCATGTTCTGTGACACCCCCATGGTTCATCACATTCG ACGCTTTGTTCATGGCCAAGGCTGTGTACAGATAATTCTGAAGGAGTTGGATTCTCCAGTGCCTGGATAT CAACATACAATTCTCACATATTCCTGGTGCAGAATCTGCAAACAAGTAACACCAGTTGTTGCTCTTTCAA ATGAATCCTGGTCTATGTCATTTGCAAAGTACCTTGAACTTCGATTTTATGGCCACCAGTACACACGCAG AGCCAACGCTGAGCCCTGCGGTCACTCTATCCACCATGATTATCACCAGTATTTCTCTTATAACCAGATG GTGGCATCTTTCAGTTACTCTCCTATTCGGCTTCTTGAAGTATGTGTTCCACTACCAAAAATATTCATTA AGCGTCAAGCCCCACTGAAGGTATCTCTTCTTCAGGACCTCAAAGACTTTTTTCAGAAGGTTTCACAGGT GTACCTAGCTGTTGATGAGAGACTTGCATCCTTGAAAACGGATACATTTAGCAAAACTAGAGAGGAAAAG ATGGAAGATATCTTTGCACAAAAGGAGATGGAAGAGGGTGAGTTTAAGAACTGGACAGAGAAGATGCAAG CAAGGCTCATGTCTTCCTCTGTGGATACCCCTCAGCAACTGCAGTCCATTTTTGAGTCACTGATTGCCAA GAAGCAAAGCCTCTGTGAGGTGCTCCAGGCGTGGAACAGCAGGTTGCAGGACCTCTTCCAGCAGGAAAAA GGTAGAAAGAGGCCTTCAGTTCCTCCCAGTCCTGGGAGACTGAGACAAGGTGAAGAAAGCAAGATAAATG CAATGGACACATCTCCAAGGAATATTTCTCCAGGACTTCACAATGGAGAAAAAGAGGATCGCTTCTTGAC AACCCTGTCCAGCCAGAGCTCTACGAGCTCCACCCACCTCCAGCTGCCCACTCCTCCCGAGGCCCTGGCC GAGCAGGTAGTGGGAGGGCCGACTGATCTGGATTCAGCCAGTGGCTCTGAAGATGTATTTGATGGTCATT TGCTGGGATCCACAGACAGCCAGGTGAAGGAAAAGTCAACCATGAAAGCCATCTTTGCTAATTTGCTTCC AGGAAACAGCTACAATCCCATTCCATTTCCTTTTGATCCAGATAAACACTACTTAATGTATGAACATGAA CGGGTGCCCATTGCTGTCTGTGAGAAAGAGCCCAGCTCCATCATTGCTTTTGCACTCAGTTGTAAAGAAT ACCGCAATGCCTTAGAGGAATTGTCCAAAGCAACTCTGCGGAACAGTGCTGAAGAAGGGCTCCCAGCCAA TAGTGCTTTAGATAACAGACCTAAGAGTAGCAGCCCTATTAGACTACCTGAAATCAGTGGAGGACAGACA AACCGCACAGTAGAAGCAGAACCTCAGCCAACCAAAAAAGCTTCAGGAATGTTGTCCTTCTTCAGAGGAA CAGCAGGGAAGAGCCCTGATCTGTCTTCCCAGAAGAGGGAGACCTTGCGAGGGGCAGACAGTGCTTACTA CCAGGTTGGGCAGGCCGGCAAGGAGGGGTTGGAGAGTCAAGGCCTGGAGCCTCAAGATGAAGTAGATGGA GGAGATACACAGAAGAAACAACTCACAAATCCTCATGTGGAACTTCAATTTTCTGATGCTAATGCCAAGT TTTACTGTCGGCTGTACTACGCGGGAGAGTTCCACAAGATGCGTGAAGTGATTCTGGGCAGCAGTGAGGA GGAATTCATCCGTTCCCTTTCTCACTCATCTCCCTGGCAGGCCCGGGGAGGCAAGTCAGGAGCTGCTTTC TATGCCACCGAAGATGATAGATTCATTCTGAAGCAAATGCCTCGTTTGGAAGTCCAGTCTTTCCTTGACT TTGCACCACACTACTTCAATTATATCACAAATGCTGTTCAACAAAAGAGGCCCACCGCCTTGGCTAAAAT TCTTGGAGTTTACAGAATTGGTTATAAGAACTCTCAGAACAACACTGAGAAGAAGTTAGATCTCCTTGTC ATGGAAAATCTTTTCTATGGGAGAAAGATGGCACAGGTTTTTGATTTGAAGGGTTCACTTAGGAATCGAA ATGTAAAAACTGACACTGGGAAAGAGAGCTGTGATGTGGTTCTGTTGGATGAAAACCTCCTAAAGATGGT TCGAGACAACCCTCTCTATATTCGTTCCCATTCCAAATCTGTGCTGAGAACCTCCATCCACAGCGACGCC CATTTCCTTTCCAGCCACCTCATTATAGACTATTCTCTGCTGGTTGGGCGAGATGACACTAGCAATGAGC TTGTGGTTGGCATCATAGATTACATTCGAACATTTACATGGGACAAAAAACTTGAGATGGTTGTGAAGTC AACAGGAATTTTAGGAGGACAAGGTAAAATGCCAACTGTGGTCTCTCCAGAGTTGTATAGGACTAGATTT TGTGAAGCAATGGACAAGTATTTCTTGATGGTGCCAGACCACTGGACAGGGTTGGATCTGAATTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR225815 representing NM_011086 Red=Cloning site Green=Tags(s) MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSP SSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLENTLPHPQESTDSRRKAEPACGGHDPRTAVQLR SLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGK FMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDD LAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRT ETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAI GQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQ IAEEGDDNLAKYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAM ERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKK FDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDV RPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIF QLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQ SPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKH QDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMY VTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVK EMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKH LDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSAC VSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGY QHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQM VASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEK MEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEK GRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALA EQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHE RVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQT NRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDG GDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEEFIRSLSHSSPWQARGGKSGAAF YATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLV MENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDA HFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRF CEAMDKYFLMVPDHWTGLDLNC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_011086
ORF Size	6156 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_011086.2, NP_035216.2
RefSeq Size	11255 bp
RefSeq ORF	6159 bp
Locus ID	18711
UniProt ID	Q9Z1T6
MW	232.5 kDa
Gene Summary	The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. The product of the reaction it catalyzes functions as an important regulator of vacuole homeostasis perhaps by controlling membrane flux to and/or from the vacuole.[UniProtKB/Swiss-Prot Function]

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