Med12 (NM_021521) Mouse Tagged ORF Clone
CAT#: MR225918
- TrueORF®
Med12 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 12 homolog (yeast) (Med12)
ORF Plasmid: tGFP
"NM_021521" in other vectors (2)
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CNY 14,456.00
Product images
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CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 230kDa; Mopa; OPA-1; Tnrc11; Trap230 |
Vector | pCMV6-Entry2 |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225918 representing NM_021521
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTTTCGGGATCTTGAGCTATGAACACCGACCCCTGAAGCGGCTGCGGCTGGGGCCTCCCGATG TGTACCCTCAAGATCCCAAACAGAAGGAGGATGAACTGACGGCTTTGAATGTAAAACAAGGTTTCAATAA CCAGCCTGCTGTCTCTGGGGACGAACATGGCAGTGCCAAGAACGTCAACTTCAATCCTGCCAAGATCAGT TCCAACTTCAGCAGCATCATCGCAGAGAAGTTAAGGTGTAATACTCTCTCTGACACTGGTCGCAGGAAGT CCCTAATGAACCAGAAGGACAACTTCTGGCTGGTGACTGCAAGATCTCAGAGTGCTATTAACACCTGGTT CACTGACCTGGCTGGCACCAAACCACTCACACACCTAGCCAAAAAGGTCCCCATTTTCAGTAAAAAGGAA GAAGTATTTGGGTATTTGGCCAAATACACGGTGCCTGTGATGCGGGCTGCCTGGCTCATTAAGATGACTT GTGCCTACTATGCAGCAATGTCTGAGACTAAGGTTAAGAAGAAAAATACTGCTGACCCCTTCACTGAATG GACTCAAATCATCACAAAGTACTTGTGGGAGCAGCTACAGAAGATGGCTGAGTACTATCGGCCAGGGCCT GCAGGAAGTGGAGGCTGTGGTTCTACTATAGGGCCTTTGCCCCATGATGTAGAGATGGCCATCAGGCAGT GGGATTACAATGAGAAGCTCGCCTTGTTCATGTTTCAGGATGGAATGCTGGACAGACATGAGTTCCTGAC TTGGGTGCTTGAGTGTTTTGAGAAAATACGCCCTGGAGAAGATGAATTGCTTAAATTGCTGCTTCCCCTA CTGCTGCGATACTCAGGGGAGTTTGTTCAGTCTGCCTATCTGTCCCGCCGCCTTGCCTACTTCTGTACCC GGAGATTGGCTCTCCAGCTGGATGGTGTGAGCAGCCACTCATCTCATGTCATAGCTGCTCAGTCAACAAG TTCTCTGCCCACTACCCCAGCACCTCAGCCCCCAACTAGCAGTACACCCTCGACTCCCTTTAGTGACCTG CTTATGTGCCCTCAGCATCGTCCCCTGGTTTTTGGCCTCAGCTGTATCCTTCAGACCATCCTTCTGTGTT GCCCTAGTGCCCTAGTTTGGCACTACTCATTGACTGATAGCCGAATTAAGACCGGCTCACCACTTGACCA CCTGCCTATTGCTCCTTCCAACTTGCCCATGCCAGAGGGTAACAGTGCCTTTACTCAGCAGGTCCGTGCA AAATTACGAGAGATCGAACAACAGATCAAGGAGCGTGGACAAGCAGTTGAGGTTCGCTGGTCTTTTGATA AGTGCCAGGAAGCTACTGCAGGTTTCACCATTGGACGGGTGCTCCATACTTTAGAAGTGCTGGATAGCCA TAGTTTCGAGCGCTCTGACTTTAGTAACTCTCTTGACTCCCTTTGTAATCGAATCTTTGGATTGGGGCCT AGTAAGGATGGTCATGAGATTTCCTCTGATGATGATGCTGTGGTATCATTATTGTGTGAATGGGCTGTAA GCTGCAAACGCTCAGGTCGGCATCGTGCTATGGTAGTAGCCAAACTCCTAGAGAAGAGACAAGCAGAAAT TGAGGCTGAGCGCTGCGGAGAATCTGAAGCAGCTGATGAGAAGGGCTCCGTCGCCTCTGGTTCCCTTTCC GCTCCTAGTGCACCCATTTTCCAGGATGTCCTCCTGCAGTTTCTGGATACACAGGCTCCCATGCTGACTG ATCCCCGAAGTGAAAGTGAGCGAGTAGAATTCTTTAACTTGGTACTGCTCTTCTGTGAACTGATCCGACA TGATGTCTTCTCCCATAATATGTACACCTGCACTCTCATCTCTCGGGGGGATCTTGCATTTGGAGCCCCT GGTCCTCGGCCTCCCTCTCCCTTTGATGATCCTACAGATGATCCAGAGCGCAAGGAGGCTGAAGGCAGCA GCAGCAGCAAGCTAGAGGACCCAGGGCTCTCTGAATCTATGGACATCGACCCTAGTTCCAGTGTGCTTTT TGAGGACATGGAAAAGCCTGATTTCTCATTGTTCTCCCCTACTATGCCTTGTGAGGGGAAGGGAAGCCCA TCCCCTGAGAAACCAGATGTCGAAAAGGAAGTAAAACCCCCAGCCAAAGAGAAGATCGAGGGGACACTTG GGATTCTGTATGACCAGCCACGACATGTGCAGTATGCCACACACTTTCCAATCCCACAGGAGGAGTCATG CAGCCATGAGTGCAACCAGCGGTTGGTCGTACTGTTTGGGGTGGGGAAGCAGCGAGATGATGCCCGCCAT GCCATCAAGAAGATTACCAAGGATATCCTGAAGGTTCTGAATCGCAAGGGGACAGCAGAAACTGACCAGC TTGCTCCTATTGTGCCTCTGAATCCTGGAGACCTGACATTCTTAGGTGGGGAAGATGGGCAGAAGCGACG ACGCAACCGGCCCGAAGCCTTCCCCACTGCTGAAGATATTTTTGCTAAGTTCCAGCACCTTTCCCATTAT GACCAACACCAGGTCACGGCTCAGGTCTCCCGGAACGTTCTGGAGCAGATCACGAGCTTCGCCCTTGGCA TGTCGTACCACTTGCCTCTGGTGCAGCATGTGCAGTTCATCTTCGACCTCATGGAATATTCACTGAGCAT CAGTGGCCTCATCGACTTTGCCATCCAGTTACTGAATGAGCTGAGTGTGGTTGAGGCGGAGCTCCTTCTC AAATCGTCGGATCTGGTGGGCAGCTACACTACCAGCCTGTGCTTATGTATCGTGGCTGTCCTTCGACACT ATCATGCCTGCCTCATCCTCAATCAGGACCAGATGGCGCAAGTGTTTGAGGGGCTCTGTGGCGTGGTGAA ACATGGAATGAACCGTTCAGATGGCTCCTCTGCAGAGCGCTGTATCCTTGCTTATCTCTATGATCTGTAT ACCTCCTGTAGCCATTTAAAGAGCAAATTTGGGGAGCTCTTCAGTGACTTTTGCTCAAAAGTGAAGAACA CCATCTACTGTAACGTGGAGCCATCGGAATCCAATATGCGCTGGGCACCGGAGTTCATGATTGACACTCT GGAGAACCCTGCCGCTCACACTTTCACCTACACGGGGCTCGGCAAGAGTCTTAGTGAGAACCCTGCTAAC CGCTATAGCTTTGTGTGCAATGCTCTTATGCACGTCTGCGTGGGCCACCATGATCCTGATAGGGTAAATG ACATCGCCATCCTTTGTGCAGAGCTGACCGGCTATTGCAAGTCCTTGAGTGCTGAGTGGCTAGGAGTACT TAAGGCCTTGTGCTGCTCCTCGAACAATGGCACTTGTGGTTTCAATGATCTCCTGTGCAACGTAGACGTC AGTGATTTGTCTTTTCATGATTCCTTGGCTACTTTTGTTGCTATCCTCATCGCCCGTCAATGTTTGCTCC TAGAAGACCTGATTCGCTGTGCAGCCATCCCTTCACTCCTCAATGCTGCTTGTAGTGAGCAGGATTCTGA GCCAGGAGCCAGGCTTACTTGCCGAATCCTCCTCCACCTGTTCAAGACGCCACAACTCAATCCTTGCCAG TCTGATGGAAACAAACCTACTGTTGGAATCCGGTCCTCCTGTGACCGCCACCTGCTGGCTGCCTCCCAGA ACCGCATCGTGGATGGGGCTGTGTTTGCTGTTCTCAAGGCTGTGTTTGTACTCGGAGATGCGGAGCTAAA AGGTTCAGGCTTTACTGTGCCGGGAGGAACAGAAGAACTTCCAGAAGAGGAGGGAGGAGGTGGTAGTAGC GGTCGGAGACAGGGTGGCCGCAACATCTCTGTGGAGACAGCAAGTCTGGATGTCTATGCCAAGTACGTGC TGCGAAGCATCTGCCAACAGGAATGGGTAGGAGAACGTTGCCTTAAGTCACTGTGTGAGGATAGCAATGA TCTACAAGACCCAGTGTTGAGTAGTGCCCAGGCCCAGCGCCTCATGCAACTTATCTGCTACCCACATCGA CTGCTGGACAATGAGGATGGAGAAAACCCCCAGCGGCAGCGCATTAAACGTATTCTCAAGAATTTAGACC AATGGACCATGCGCCAGTCCTCTTTGGAGCTACAGTTGATGATCAAGCAGACCCCCAACACTGAGATGAA CTCTCTCTTGGAGAACATTGCCAAGGCCACAATCGAGGTTTTCCAACAGTCAGCAGAGACGGGGTCATCT TCTGGAAGTACAGCAAGCAACATGCCCAGCAGCAGCAAGACCAAACCTGTGCTCAGCTCTCTAGAGCGAT CTGGTGTATGGTTGGTGGCTCCTCTCATTGCCAAACTGCCCACTTCAGTCCAGGGCCATGTATTAAAAGC TGCTGGGGAGGAACTGGAAAAGGGTCAGCACCTGGGCTCTTCTTCCCGTAAAGAACGAGATCGACAGAAA CAGAAGAGCATGTCCCTGTTGAGCCAACAGCCTTTCTTATCACTGGTGCTAACATGTCTGAAAGGACAGG ATGAGCAGCGCGAGGGACTCCTGGCCTCCCTCCACAGCCAGGTGCACCAGATTGTGATTAATTGGCGAGA AAACCAGTACTTAGATGATTGCAAACCAAAGCAGCTAATGCATGAGGCACTCAAACTGCGGCTCAACCTG GTGGGGGGCATGTTTGACACTGTGCAGCGTAGTACCCAGCAGACTACAGAGTGGGCCCAGCTTCTCCTTG AGATCATCATCAGCGGCACTGTGGACATGCAGTCTAACAATGAGCTCTTCACTACTGTGTTGGACATGCT GAGCGTGCTTATCAACGGAACGTTGGCTGCAGACATGTCCAGTATCTCGCAAGGCAGCATGGAGGAAAAC AAGCGTGCATATATGAACCTGGTGAAGAAGCTTCAGAAGGACTTGGGGGAGCGCCAATCAGACAGTCTGG AGAAGGTTCACCAACTGTTGCCACTACCCAAGCAGAACCGAGATGTCATAACCTGTGAGCCACAGGGCTC CCTTATTGACACCAAGGGCAACAAGATTGCTGGCTTCGATTCCATCTTCAAGAAGGAGGGTCTACAAGTT TCTACCAAACAAAAGATCTCTCCCTGGGAGCTTTTTGAGGGCCTGAAGCCATCAACAGCACCACTGTCAT GGGCCTGGTTTGGCACAGTCCGAGTGGACCGCAGAGTGGCACGAGGGGAGGAGCAGCAGCGGCTGTTGCT CTATCATACCCACCTGAGGCCTCGACCCAGAGCCTATTACCTGGAACCACTACCTCTGCCCCCAGAAGAT GAGGAGCCACCAGCCCCTGCCCTACTAGAGCCTGAGAAAAAGGCTCCTGAGCCCCCCAAGACTGACAAAC CAGGGGCTGCTCCTCCGAGCACTGAGGAGCGCAAAAAGAAGTCTACCAAGGGCAAAAAACGCAGCCAGCC AGCCACCAAGAACGAGGACTATGGCATGGGGCCAGGTCGGAGTGGCCCCTATGGTGTGACAGTGCCTCCA GACCTTCTACACCATGCAAATCCTGGTTCTATATCCCACCTTAGCTACAGGCAAAGCTCCATGGGCCTGT ATACCCAAAACCAGCCACTACCTGCTGGTGGCCCTCGTGTGGATCCATACCGCCCCGTGCGATTACCAAT GCAAAAGCTGCCAACTCGACCAACTTATCCCGGTGTGCTGCCTACAACTATGTCTACTGTCATGGGCCTA GAACCCTCTTCTTATAAGACATCTGTATACCGGCAGCAGCAACCCACAGTGCCCCAGGGACAGCGCCTTC GCCAACAGCTCCAGGCAAAGATACAGAGTCAGGGGATGTTGGGACAGTCATCTGTCCATCAGATGACCCC TAGTTCTTCCTATGGTTTGCAGACTTCCCAGGGCTATACATCCTATGTTTCTCATGTGGGATTGCAGCAA CACACAGGCCCTGCAGGTACCATGGTGCCCCCCAGCTACTCCAGCCAGCCTTATCAGAGCACCCACCCTT CTACCAATCCTACTCTTGTAGATCCTACCCGCCACCTGCAACAGCGGCCCAGTGGCTATGTGCATCAGCA GGCCCCAACCTATGGGCATGGACTGACTTCCACTCAAAGGTTTTCACACCAGACACTGCAGCAGACACCC ATGATGGGTACCATGACTCCGTTGAGTGCCCAGGGTGTCCAGGCAGGCGTCCGTTCAACTTCCATCCTGC CTGAGCAGCAGCAACAACAACAGCAGCAGCAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAACAACA ACAGCAGCAGCAGCAGCAGCAACAACAACAGCAGTACCATATCCGACAGCAACAGCAGCAGCAGCAGATG CTACGGCAACAGCAGCAACAACAGCAACAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAACAACAACAGC AACAGCAGCAGCAGCAGCCACACCAGCAGCAGCAGCAGGCAGCTCCTCCCCAACCCCAGCCCCAGTCCCA GCCCCAGTTCCAGCGCCAGGGCCTGCAGCAGACCCAGCAGCAGCAACAGACAGCAGCTTTGGTCCGGCAA CTTCAACAACAGCTCTCTAATACCCAGCCACAGCCCAGCACCAACATATTTGGACGCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225918 representing NM_021521
Red=Cloning site Green=Tags(s) MAAFGILSYEHRPLKRLRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKIS SNFSSIIAEKLRCNTLSDTGRRKSLMNQKDNFWLVTARSQSAINTWFTDLAGTKPLTHLAKKVPIFSKKE EVFGYLAKYTVPVMRAAWLIKMTCAYYAAMSETKVKKKNTADPFTEWTQIITKYLWEQLQKMAEYYRPGP AGSGGCGSTIGPLPHDVEMAIRQWDYNEKLALFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPL LLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVIAAQSTSSLPTTPAPQPPTSSTPSTPFSDL LMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRA KLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGP SKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSVASGSLS APSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAP GPRPPSPFDDPTDDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSP SPEKPDVEKEVKPPAKEKIEGTLGILYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARH AIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHY DQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLL KSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLY TSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPAN RYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDV SDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQ SDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVPGGTEELPEEEGGGGSS GRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHR LLDNEDGENPQRQRIKRILKNLDQWTMRQSSLELQLMIKQTPNTEMNSLLENIAKATIEVFQQSAETGSS SGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQK QKSMSLLSQQPFLSLVLTCLKGQDEQREGLLASLHSQVHQIVINWRENQYLDDCKPKQLMHEALKLRLNL VGGMFDTVQRSTQQTTEWAQLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEEN KRAYMNLVKKLQKDLGERQSDSLEKVHQLLPLPKQNRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQV STKQKISPWELFEGLKPSTAPLSWAWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPED EEPPAPALLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKNEDYGMGPGRSGPYGVTVPP DLLHHANPGSISHLSYRQSSMGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMSTVMGL EPSSYKTSVYRQQQPTVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTSYVSHVGLQQ HTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTP MMGTMTPLSAQGVQAGVRSTSILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQQM LRQQQQQQQQQQQQQQQQQQQQQQQQQQQPHQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQ LQQQLSNTQPQPSTNIFGRY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_021521 |
ORF Size | 6570 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021521.2, NP_067496.2 |
RefSeq Size | 8464 bp |
RefSeq ORF | 6573 bp |
Locus ID | 59024 |
UniProt ID | A2AGH6 |
MW | 245 kDa |
Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC225218 | Med12 (untagged) - Mouse mediator of RNA polymerase II transcription, subunit 12 homolog (yeast) (Med12), (10ug) |
CNY 35,250.00 |
|
MG225918 | Med12 (tGFP-tagged) - Mouse mediator of RNA polymerase II transcription subunit 12 homolog (yeast) (Med12), (10ug) |
CNY 26,410.00 |