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Cep162 (NM_199316) Mouse Tagged ORF Clone
CAT＃: MR225968
- TrueORF®
4922501C03Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4922501C03 gene (4922501C03Rik)
ORF Plasmid: tGFP
"NM_199316" in other vectors (4)
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CNY 9,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4922501C03Rik; mKIAA1009 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225968 representing NM_199316
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCACTATTTCAAAGTAGACCTTGATGAAGAATTTGAACGGTTTATGAAGGAGCTTTCAGATGATT CTTTTGAAAATTCAAATAAAACACCTAGACAACCTAATGAAGACAACAAAGAAATGAAGAAGAAAGACCC AGTGCCTTGGTGGATAGCCGAAGATGATTTTGAAGATGATGGATTGCTTGGAACAAATGTGAGCTATTTG AAGACAAAGAAGACATATCAGCCTGTTATGGACACAGAGGAGGAATCTGCTGAAAAAGTTCAGTTTCTTA AGAGCAGTGGAACCTCCATCTTAAGTGTTGACAGCTTAGAAGCTAATGAATTGGTTGTTTCTGAGCCCCA TCATAGCACTCTTGGTTTGGGCTTGGACACGTTAGAAGAACAAGAGGAGAAAGAGCAGTTCTTTGCCAGG CTTGAGAAAGGCTTGACGTCTTCTATTGATTATTCGAAATTAAATCAAGAACTAGATTCTGACGACTCTG CACAATTGAAAGCTTTACATAGATATCCACGTAATACAGAGCCAGCTGAAGATGGATGTGAGAATGAATC AGAACAAGAGGAACTGCCAGAAACGTACAGTGATGATTTTGAAGATGCTGAGGATGCTGATGATCCTTTG ATTACTAAAGATGAAGAGACCCATCCCAAAGAAAACTCTGAGTCAGGAAAAGACAGCTTTCCCAAACAGG AGGAAGAGAAAACTGGCATGCTGGCTAATGTTGTGCTGCTTGATTCCTTTGACTCTGTTGAAGATGTTGG CCTTAGCAGTCAAGAGAAAGCAACCCCTAAAGCAAAGGCCCCTCCAGAAATAACAGACGACGGACCAGCA GAAACAGGTGTTCCTTATGGACAAAGCAGTGGTGACACTGAAGCCCTGCACCAAGCTTACTGTCATGTAG CTCATTCTCTGGGAGACACAGGCGAACCAAGAATTGAGGCTAGCACTGTGCAAACTGTCAGGAGCTCCAT AAAAGATGGTCTTCAAGAAAACGAAGAGTCTTCAAAGAACGTCTCTACCACCGAGTCCGATTTGCCCACA GTGGAGGAGCTGATGCAGCCTATCAGGATCGATTCCTATGGGATCAGGGCCTTTGACTTGCAGCCTATCA GCCTTAAGAAAGCAACGGACAGTAAAGAAGCTGAATCTGTAGGCTCTTTACCCCTGAAGACAAACACAAA CACTGTGTCTCAAGACACTAGACATGCGATCCAATTTCCCCACAAACACGATGAGAGTGTGGTTCTACAC AGGACAGCAGATGAGGGCATGGGCAGCAGCTGTCCAGCTACAGAGGAGCATTTGGACAAAATGTATCTGG AGATTTTGAAGAAGAAAACATCAGTTAACCCCTCACTATTACCTCAGGATGACAAAATGAACCAGACTTC CAGGTCCCAGCTCGGGGCTGGGGAAGAGGTGCCTGTCATTGGTAAACAGGTTCCATGTAAGAAGGCCAGA AGCACACCTTCTTTGCCTAAGAGAAAGCCACAGAGTGGACTGTACGCATCAGCTCGGAGCTCAGGCTACG GCAAACCCAGTTCACCACTCCAGTTATTTTCAGCCCTTGAAAAGAAAACTTCAAAGGATAATACAAAAAC TAAAAGTGTGAGATCCATTCCCACCTCAAATCAATTTAGAAAAAGAGAAATACTATCTGGAACAAAACTC ATCAAGCCTGCAGCTTCGAATAAACCATCCCCTCACCGTGAAGGTAGCCCAGCTACACCCAAGAGACCTG AAGACCCCTCAGATGATTCCTTTGTTCAGTTACAGACTGAGCCTTTGGGATCCTACGGTGGGAACAGAGA GAAAGAGTTACTAATGTTGAAAAGAGCTCAGGATGCGGAGGAAAAATGGACGGGTGCACAAGCACTGATG GAGCAAATGAAAATGACATTCTGTGAGAAGGAGAAGGAACTGGAGAACACAGTGGAAAGCCTCAAGAGGC AACAGGAGAGAGAGCTCTTCAGACTGAACCAAGAGAACTACATTCTCCAGGCCAAGTTAAGTAGCTTTGA AGAAACAAGCAGAAAACAGAGGTGGCTACAATTTGGAGAAACATCTGACCCCCTCACTGGAGAGAAACTG AAACAAATTCAAAAAGAGATACAAGAGCAAGAGACACTTCTTCAAGGCTACCAGCAGGAAAATGAAAGGT TGTATAATCAAGTAAAAGATCTTCAGGAACAAAATAAGAAAAATGAAGAGCGAATGTTTAAAGAAAATCA GAATTTATTTAGTGAGTTGGCCTCTTTAAAAGAACAGATGCACAAAAATCATTTTCTGTCTCAAGCAGTT GAAAATACAGAACCCACAAAAAACCAGAGTTTTACAGATCTTCTAGCAGAATTACGGGCGGCACAGAAAG AAAAAAACCACCTCATGGAAGACATCAAAAGACTGAAGCAAGACAAACAAGCTCTGGAGGTAGACTTGGA AAAAGTTAAGAGAGAGAGGGACCAAGCCAAAGATCAAATCGCCTATGCCACAGGTGAAAAATTATATGAA ATAAAGATTTTAGAAGAAACACATAAACAAGAAGTGAGTCGTCTACAAAAGCGACTACAGTGGTACGCTG AAAACCAGGAGCTTCTGGATAGAGACGCAGCTCGCCTCCGAGAGGCCAATGAAGAGACTGAGAAGCTGAG ATTAGAGATTGAAAAACTGAAAACTGAGTCTGGGAGCCCAGCTACTCAGCAGAGGCTGCGCTCAAAGGAA AGAGCTCTTGATGCCAAAAGAATTCAGGATCTGGAGCGGCAGGTTAAAGAAATGGAAGGGATTCTGAAGA GAAGGTATCCCAACTCTTTGCCTGCTTTGATACTGGCTGCCTCGGCAGCTGGTGATTCGGTAGATAGAAA TACAGTGGAGTTTATGGAGAGAAGAATAAAAAAGCTAGAAGCTGATCTGGAGGGCAAAGATGAAGAAGCC AAGAAAAGCCTTCGCACCATGGAACAGCAGTTTCAGAAAATGAAGATTCAGTACGAACAGCGGCTAGAGG AGCAGGAACAGCTGCTTGCCCACAGGCAGAAGGAAGCTCCGCAGAGCCAGCGTAACAGCTCATCCAGGCT GAAGGCCCTTGAGACAGAACTTGGGGACATAAAGGAAGCCCATCAGATCACTGTAAGAAAGCTGGAAGCT GAAATAGATGTTCTCAAACATCAAAATGCTGACTTAGAACACAAGAAGAATGACAAAGGAGATCAGGGCC TCCAGTCCATAGAGTTCCAGGTGGAGCAGGCGCAGGCCAGGGCAAAACTGGCGAGACTCAATGAAGAGCT GGCAGCGAAGGGGAGAGAGATACAAGACCTTACAAAGACCGTGGAGAGGCTCCAGAAGGAGAGAAGGATG ATGCTGTCGAGGCAGATCCCCAGGAGCAGAGAGGAGACGGCTGCCAAAAGGCTGAAGAAAGACCCAAACA GAGGACATGGGAATGCTTTCCCTGAAACCCTGGATGGCAAACTGTATCACCCACACACCTTTACTGACTC TCATATTTCGGAGGTCTTAGAAGAAAACTACAGATTGAGAAGTGAGCTGGAGGGCTTAATTTTAGAGAGA AGTAAGCTGAAGATGGAATCTGAGGCAGCAGTATGCCAACTTGAAAACTCCATGAAAAGGGTCAAGGACG ACGCTGCAGCGCACATTGCGTCCCTCAAAGCATCTCATGAGAGGGAAATAGAGAAACTCCTTTGCCAAAA TGCAATAGAAAACTCCTCTTCCAAAGTAGCTGAACTGAATCGCAAAATCGCAACTCAAGAGGTACTTCTA AAACATTTCCAAGGTCAAGTTAATGAGCTGCAGGGTAAACAGGAATCCCTTGCAGTTTCTCAAGTTCGAG AAGAAATCCTACAGAAACAGATTACAAAACTCTTGGAAGAATTGAAAGAAGCCAAAGAAAACCACACACC AGAGATGAAGCATTTCATGGGCTTAGAAAGGAAGATCAAGCAGATGGAGATGAGGCATAGGCAGAGAGAG CAGGAGCTCCAACAGATAATACAGCAAACACGCCAAGTAGTAGAAACAGAGCAAAACAAGGAAGTTGAGA AATGGAAAAGACTTGCACAGTTAAAGAATCGGGAGCTGGACAAGTTCCGCACAGAGCTGGACTCCATACT GGACGTTCTGAGGGAGCTGCACCGACAAGGGGTTGTGGTCCCCATGGCTCTCGCAGGCGAAGAGAACACA GCAGAGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225968 representing NM_199316
Red=Cloning site Green=Tags(s) MAHYFKVDLDEEFERFMKELSDDSFENSNKTPRQPNEDNKEMKKKDPVPWWIAEDDFEDDGLLGTNVSYL KTKKTYQPVMDTEEESAEKVQFLKSSGTSILSVDSLEANELVVSEPHHSTLGLGLDTLEEQEEKEQFFAR LEKGLTSSIDYSKLNQELDSDDSAQLKALHRYPRNTEPAEDGCENESEQEELPETYSDDFEDAEDADDPL ITKDEETHPKENSESGKDSFPKQEEEKTGMLANVVLLDSFDSVEDVGLSSQEKATPKAKAPPEITDDGPA ETGVPYGQSSGDTEALHQAYCHVAHSLGDTGEPRIEASTVQTVRSSIKDGLQENEESSKNVSTTESDLPT VEELMQPIRIDSYGIRAFDLQPISLKKATDSKEAESVGSLPLKTNTNTVSQDTRHAIQFPHKHDESVVLH RTADEGMGSSCPATEEHLDKMYLEILKKKTSVNPSLLPQDDKMNQTSRSQLGAGEEVPVIGKQVPCKKAR STPSLPKRKPQSGLYASARSSGYGKPSSPLQLFSALEKKTSKDNTKTKSVRSIPTSNQFRKREILSGTKL IKPAASNKPSPHREGSPATPKRPEDPSDDSFVQLQTEPLGSYGGNREKELLMLKRAQDAEEKWTGAQALM EQMKMTFCEKEKELENTVESLKRQQERELFRLNQENYILQAKLSSFEETSRKQRWLQFGETSDPLTGEKL KQIQKEIQEQETLLQGYQQENERLYNQVKDLQEQNKKNEERMFKENQNLFSELASLKEQMHKNHFLSQAV ENTEPTKNQSFTDLLAELRAAQKEKNHLMEDIKRLKQDKQALEVDLEKVKRERDQAKDQIAYATGEKLYE IKILEETHKQEVSRLQKRLQWYAENQELLDRDAARLREANEETEKLRLEIEKLKTESGSPATQQRLRSKE RALDAKRIQDLERQVKEMEGILKRRYPNSLPALILAASAAGDSVDRNTVEFMERRIKKLEADLEGKDEEA KKSLRTMEQQFQKMKIQYEQRLEEQEQLLAHRQKEAPQSQRNSSSRLKALETELGDIKEAHQITVRKLEA EIDVLKHQNADLEHKKNDKGDQGLQSIEFQVEQAQARAKLARLNEELAAKGREIQDLTKTVERLQKERRM MLSRQIPRSREETAAKRLKKDPNRGHGNAFPETLDGKLYHPHTFTDSHISEVLEENYRLRSELEGLILER SKLKMESEAAVCQLENSMKRVKDDAAAHIASLKASHEREIEKLLCQNAIENSSSKVAELNRKIATQEVLL KHFQGQVNELQGKQESLAVSQVREEILQKQITKLLEELKEAKENHTPEMKHFMGLERKIKQMEMRHRQRE QELQQIIQQTRQVVETEQNKEVEKWKRLAQLKNRELDKFRTELDSILDVLRELHRQGVVVPMALAGEENT AEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_199316 |
| ORF Size | 4209 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_199316.2, NP_955020.2 |
| RefSeq Size | 5295 bp |
| RefSeq ORF | 4212 bp |
| Locus ID | 382090 |
| UniProt ID | Q6ZQ06 |
| MW | 160.9 kDa |
| Gene Summary | Required to promote assembly of the transition zone in primary cilia. Acts by specifically recognizing and binding the axonemal microtubule. Localizes to the distal ends of centrioles before ciliogenesis and directly binds to axonemal microtubule, thereby promoting and restricting transition zone formation specifically at the cilia base. Required to mediate CEP290 association with microtubules (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224409 | 4922501C03Rik (untagged) - Mouse RIKEN cDNA 4922501C03 gene (4922501C03Rik), (10ug) |
CNY 18,050.00 |
|
| MG225968 | 4922501C03Rik (tGFP-tagged) - Mouse RIKEN cDNA 4922501C03 gene (4922501C03Rik), (10ug) |
CNY 11,880.00 |
|
| MR225968L3 | Lenti ORF clone of 4922501C03Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4922501C03 gene (4922501C03Rik) |
CNY 12,730.00 |
|
| MR225968L4 | Lenti ORF clone of 4922501C03Rik (mGFP-tagged) - Mouse RIKEN cDNA 4922501C03 gene (4922501C03Rik) |
CNY 12,730.00 |
