Cdk5rap2 (NM_145990) Mouse Tagged ORF Clone
CAT#: MR226032
- TrueORF®
Cdk5rap2 (Myc-DDK-tagged) - Mouse CDK5 regulatory subunit associated protein 2 (Cdk5rap2)
ORF Plasmid: tGFP
"NM_145990" in other vectors (2)
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CNY 12,248.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2900018K03Rik; an; mKIAA1633 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226032 representing NM_145990
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCGGGGATGGAAGAGGAAGGCGCCCTGCCTGGGACCCTCAGCGGTTGCAGTGGCCTTCACCCTG TTCTGCCAAGTGACCTGGATGTCATCAGTGACACTTCTGGCTTGGGAAATGGAGTGCTCCCAAGCATGTC AGAAGAAAAGGTTTCTCCCACCAGAGCCCGGAACATGAAGGACTTTGAAAACCAAATCACTGAGCTGAAG AAGGAGAACTTCAATCTAAAGCTCCGGATCTACTTCCTGGAGGAGAGAATCCAGCAGGAGTTTGCTGGCC CCACCGAGCACATCTACAAGACGAACATTGAACTCAAGGTGGAAGTGGAAAGTCTGAAGCGCGAGCTCCA GGAGAAAGATCAGTTGCTCGTCAAAGCCTCCAAAGCGGTGGAGAGCCTAGCAGAACGGGGTGGTTCTGAA GTCCAGCGGGTGAAAGAAGATGCAAGGAAGAAGGTGCAGCAGGTGGAAGACCTCCTAACGAAAAGGATAC ATCTTCTAGAAGAAGATGTGAAAGCCGCCCAGGCAGAACTGGAGAAGGCCTTTGCGGGGACAGAAACAGA GAAGGCTCTTCGGCTCAGCTTAGAAAGCAAGCTCTCGGCGATGAAGAAGATGCAGGAGGGGGACCTGGAG ATGACTCTGGCTCTGGAAGAGAAGGACAGACTGATTGAGGAGTTGAAGCTGTCTTTGAAGAGCAAAGAAG CTTTAATTCAGTGCCTTAAAGAGGAAAAATCTCAGATGGCATCTCCTGACGAGAACGTGTCATCTGGAGA ACTCCGAGGACTTTCTGCCACTCTGAGGGAAGAAAAAGAGAGAGACGCTGAGGAATGGCAGAAGGAGAGA AACCACTTTGAAGAGAGGATCCAGGCACTTCAGGAGGACCTGAGAGAGAAGGAAAGAGAAATTGCTACAG AGAAGAAAAATAACTTAAAGAGTTATAAAGCCATTCAGGGTCTAACCATGGCATTAAAATCAAAGGAAAG AGAGGTTGAAGAACTTGATTCCAAAATCAAAGAGGTCACTACGGACTCCACCAAAGGCAGAGAGGATCCC TTAAAAACCCAGATCCCCAGATTCCAGCTAAGAGAGGGGTCTGAGGACTGTGAAGCTGCGCTGGTAGAGA AGGAAGCCCTATTGGCCAAGCTACACTCAGAAAATGTCACAAAGAGCACTGAGAACCATAGGTTGCTCAG GAATGTTAAAAAGGTCACCCAGGAGTTGAATGACTTGAAGAAGGAGAAGTTGAGACTAGAGCAGGCCCTG GAGGAAGCCCACCAAGAGGGGAACAGAGGAGCCCGAACCATTCACGACCTTAGAAATGAAGTTGAGAAAC TACGCAAGGAAGTGAGTGAGAGAGAGAAGGCTGTGGAAAAACATTACAAGAGTCTTCCAGGTGAAAGCAA GACAAAGTTTCATACTCAAGAACAAGTGGTCAGAAGTCTGACAGGCAGTGGCAGTCAGGAGGACCTGCTG CTTCAGAAATCCAATGAAAAAGATTTGGAAGCAATACAGCAGAACTGCTATTTAATGACTGCAGAGGAGC TCAAGTTTGGGAGCGATGGCTTAATAACAGAAAAGTGTTCTCAGCAGTCACCAGACAGCAAACTCATCTT CTCCAAGGAAAAGCAACAATCAGAGTATGAAGGGCTGACTGGGGACTTAAAAGCAGAACAGAACATCTAT GCCCATCTGGCAAAGACTCAGGACACAGACAGTGTCAGCAACCTGCAGGCTGAGCTGAAAGAGGTCCTTG CCCTTCGGAAGCAGCTGGAGCAGGATGTTCTTGCCTACCGGAACCTGCAGAAGGCCCTGCAGGAGCAGCT CAGTGAGATTCGGAGTCGAGAAGAAGAGCCGTTTTCATTTTATAGCGATCAAACATCGTATCTGAGTATT TGCCTTGAAGAGCACAATCAGTTCCAATTGGAGCATTTTTCTCAAGAAGAACTCAAGAAAAAGGTCAGTG ACCTCATACAGCTTGTGAAGGACCTGCACACAGACAACCAGCACCTGAAGAAAACCATTTTTGATCTCTC TTCTGTGGGTTTCCAGGGAAGTGACAGACTTGAGTTAACAAAACAAGAAGAGCTTGTGGCTAGCAAGGAG GATGAGGACACACTCAAATTTGAAGCGGACGTTGAGACTCCTTTCCAGAGTGACCAGCATTTGGAGCAAA GTCGTGAGATCATGGAGGACTATGCTGAGGGTGGCTGCAAAAGTGGATATGGAAGGCACATGGATTCCAA TATTTTAGGTCATGATGGGGCCCAAACTCCTGGTGCCTCTGAAGAGCACACCCTAGAAGATGAGCTCCTG GGCCTGCTTGCCACTCTCTTCAGTAAGAAGGCCACACCGTTGCTGGAATCCAGGCCAGATCTTCTGAAAG CACTCGGTGCCCTACTTTTGGAACGCATATGCTTGGCAGAGCAGGGTCGTCCTGGAGATCACCTTGATAG TAAAACTGAGAAGGCACTTCAGCAAGTAGCTGTTCAGTTGAGAGATGAACTTGGCCATTCCTTCCCTGCC AACTCGTTCTCCAAGTCATACAATGAAGTCAAGTCAATGTGGGGAAATTGGCTGGTAAAGACAGGAGACG AAGACACGGTGGAGCTGAAGAGTGTTTCAGTGCAGACCATGGCCATAGAGGACACCCCGCATGGATTCAA ACCTCAAAGCAAGAGAGATGCTTGGGCAGAGAAACAAGAAGAGGCCATTTTCAGCACTGAGCTTGAGTCA GAGGCGTTGGGTGAGATGCCAGGGCAACAGGCCACTCACCTCTCATTCCCCAGTGCCATCAACCCGGATG CCGAGAAGACGGGCTTGTTGATCCAATTAAAGACACCTGAGTTATTGGAAAATTTATATAATCTTCCTGC TTCGCCAGAGGTGGTGGTGGCTCAACTCCAAGGCCAGGTTCTAGAGCTGCAGAGGGAGCTGAAAGAATTC AAAACCCGCAACAAGCAACTCCATGAGAAGCTAATTCTGGCAGAGGCAATGATGGAGGGGCTGCCGGTGC CCAACTCTGCGCTGGTGAATGTGCCTGCAGCCCAGGCTGTTGTGAGAACAGCCTTCCAGGACAACCCAGG CGAGCAAGAAGGACCTGAAACCACACAGTCTGCTGGGAGAGACAAGGACATGGACAGTGACCAATATACA AGCTTTGAGATCGATTCTGAAATCTGTCCACCTGATGACCTTGCCTTATTACCAGCATGCAAAGAGAATC TTGAAGACCTCCTAGGCCCATCCTCCATAGCCACTTACCTGGATTCCAAGAGTCAGCTCTCTGTTAAAGT CAGCGTGAATGGAACTGACCAGTCTGAGAATATCAATATCCCTGATGACACAGAAGACTTAAAACAGAAA ATCCACGACTTGCAAACCGAGCTGGAAGGCTACCGGAACATCATTGTCCAGCTACTGAAGCACTCTCAGT GCAGTGAGGCTATTATCACAGTTTTGTGTGGGACAGAAGGGGCCCAAGATGGCTTGAACAAGCCTAAGGG CCATATTGATGAGGAAGAGATGACATTTTCAAGTTTGCACCAAGTACGATATGTGAAGCATATGAAAATC CTCCGTCCACTAACCCCGGAGATGATTGATGGCAAGATGCTAGAGAGCCTCAAGCAGCAGCTGGTGGACC AGGAGCAGGAGCTACAGAAGGAGCAGGACTTGAACTTGGAACTTTTCGGTGAAATCCATGACCTGCAGAA CAAGTTCCAAGACCTCTCGCCATCAAGATACGATTCATTGGTTCAGTCCCAAGCGAGGGAGCTCTCCCTT CAGCGACAGCAAATTAAGGATAGCCATGGCATCTGTGTCATTTACCGTCAACACATGAGCACCATGATCA AGGCGTTTGAGGAGCTTCTGCAGGCCAGTGATGTGGACAGTTGCGTGGCCGAGGGCTTCCGGGAGCAGCT GACACAGTGTGCTGGACTGCTAGAGCAACTGGAGAGGCTGTTTCTCCATGGAAAATCAGCCAGAGTGGAA CCACATCCCCAGAATGAACTGCTCAAGGGGTTAAGGACTGTGGAAGGCAACTTACCCTACCACCATCTCC TGCCTGAATCTCCTGAGCCTTCTGCCTCCCATGCTCTGTCAGATGATGAAATGTCTGAGAAGTCCTTCCT GTCTAGAGACCCGAAGCCAGACAGCGATACTGAGAAGTACCCAGCAATGGCGAGCCATTTTCCTCAAGAC TTACTAATGGAACACATACAGGAAATTCGAACTTTGAGAAAGCATTTGGAAGAATCTATTAAAACAAATG AGAAGCTCCGGAAACAGCTGGAACGGCAGGGGTCTGAGACTGACCAAGGCTCCAGAAATGTTTCTGCTTG TGGCTTAGCACTGCACAGCTCTCTGACGTCAGAGATACATTTCCTGAGGAAGCAGAACGAGGCCCTCAGT ATGATGCTCGAGAAGGGGTCCAAAGATAAACAGAAAGAGAGTGAAAAATTACGTGAGTCCCTGGCTAGGA AAGCTGAGAGTCTTGAGCAACTTCAGCTAGAGTACACCAGTGTAAGGGAAGAAAATGAAAGGTTACAGAG AGACATCATTGAGAAGGAGAGACACAACCAGGAGCTGACTGAGGAGGTCTGCAGCAGCCGCCAGGAACTG AGCAGGGTGCAGGAAGAGGCGAAGTCAAGACAGCAGCTGCTCTCACAGAAGGACAAGCTGCTTCAGTCCC TCCAAATGGAGCTCAAGGTGTATGAGAAACTGGCCGAGGAACATCCGAGGCTACAGCAAGATGGCAGCAA ATGTCCTGAGGCCAGCGACAATTCATTTGATCTATTTGAATCCACCCAGGCAATGGCACCTAAATCAGCT TCTGAGACTCCTCTGCTCTCAGGAACTGATGTTGACTCGCTCTCCTGTGACAGTACCAGTTCTGCCACCA GCCCTACTAGCATGCCCTGCCTGGTTGCTGGCCACCACATGTGGGCCAGCAAGAGTGGCCACCATATGCT GGGCCTGATTGAGGACTATGATGCCCTCTATAAGCAGATCAGCTGGGGCCAGACACTGCTTGCCAAGATG GATGTTCAAACCCAAGAGGCTCTGAGCCCCACAAGTCACAAGCTGGGACCAAAGGGTTCTTCATCCGTGC CTCTGAGCAAGTTTCTTTCCAGTATGAACACAGCCAAGCTGGTCCTGGAAAAAGCATCCAGGTTGCTGAA GCTCTTCTGGAGGGTCTCAGTCCCCACCAACGGCCAGTGTTCCCTTCACTGTGAGCAGATTGGAGAAATG AAGGCAGAGAACACGAAACTACACAAAAAATTGTTTGAACAAGAAAAGAAGCTGCAGAACACAGCAAAAC TATTGCAGCAGAGCAAGCACCAGGAGAAAGTCATCTTTGATCAGTTGGTCATCACCCACCAAGTCCTTCG GAAGGCCAGGGGAAACCTGGAGCTCAGGCCTGGGGCCACCCGTCCAGGAGCATCCAGTCCCAGCAGACCG GGCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226032 representing NM_145990
Red=Cloning site Green=Tags(s) MDSGMEEEGALPGTLSGCSGLHPVLPSDLDVISDTSGLGNGVLPSMSEEKVSPTRARNMKDFENQITELK KENFNLKLRIYFLEERIQQEFAGPTEHIYKTNIELKVEVESLKRELQEKDQLLVKASKAVESLAERGGSE VQRVKEDARKKVQQVEDLLTKRIHLLEEDVKAAQAELEKAFAGTETEKALRLSLESKLSAMKKMQEGDLE MTLALEEKDRLIEELKLSLKSKEALIQCLKEEKSQMASPDENVSSGELRGLSATLREEKERDAEEWQKER NHFEERIQALQEDLREKEREIATEKKNNLKSYKAIQGLTMALKSKEREVEELDSKIKEVTTDSTKGREDP LKTQIPRFQLREGSEDCEAALVEKEALLAKLHSENVTKSTENHRLLRNVKKVTQELNDLKKEKLRLEQAL EEAHQEGNRGARTIHDLRNEVEKLRKEVSEREKAVEKHYKSLPGESKTKFHTQEQVVRSLTGSGSQEDLL LQKSNEKDLEAIQQNCYLMTAEELKFGSDGLITEKCSQQSPDSKLIFSKEKQQSEYEGLTGDLKAEQNIY AHLAKTQDTDSVSNLQAELKEVLALRKQLEQDVLAYRNLQKALQEQLSEIRSREEEPFSFYSDQTSYLSI CLEEHNQFQLEHFSQEELKKKVSDLIQLVKDLHTDNQHLKKTIFDLSSVGFQGSDRLELTKQEELVASKE DEDTLKFEADVETPFQSDQHLEQSREIMEDYAEGGCKSGYGRHMDSNILGHDGAQTPGASEEHTLEDELL GLLATLFSKKATPLLESRPDLLKALGALLLERICLAEQGRPGDHLDSKTEKALQQVAVQLRDELGHSFPA NSFSKSYNEVKSMWGNWLVKTGDEDTVELKSVSVQTMAIEDTPHGFKPQSKRDAWAEKQEEAIFSTELES EALGEMPGQQATHLSFPSAINPDAEKTGLLIQLKTPELLENLYNLPASPEVVVAQLQGQVLELQRELKEF KTRNKQLHEKLILAEAMMEGLPVPNSALVNVPAAQAVVRTAFQDNPGEQEGPETTQSAGRDKDMDSDQYT SFEIDSEICPPDDLALLPACKENLEDLLGPSSIATYLDSKSQLSVKVSVNGTDQSENINIPDDTEDLKQK IHDLQTELEGYRNIIVQLLKHSQCSEAIITVLCGTEGAQDGLNKPKGHIDEEEMTFSSLHQVRYVKHMKI LRPLTPEMIDGKMLESLKQQLVDQEQELQKEQDLNLELFGEIHDLQNKFQDLSPSRYDSLVQSQARELSL QRQQIKDSHGICVIYRQHMSTMIKAFEELLQASDVDSCVAEGFREQLTQCAGLLEQLERLFLHGKSARVE PHPQNELLKGLRTVEGNLPYHHLLPESPEPSASHALSDDEMSEKSFLSRDPKPDSDTEKYPAMASHFPQD LLMEHIQEIRTLRKHLEESIKTNEKLRKQLERQGSETDQGSRNVSACGLALHSSLTSEIHFLRKQNEALS MMLEKGSKDKQKESEKLRESLARKAESLEQLQLEYTSVREENERLQRDIIEKERHNQELTEEVCSSRQEL SRVQEEAKSRQQLLSQKDKLLQSLQMELKVYEKLAEEHPRLQQDGSKCPEASDNSFDLFESTQAMAPKSA SETPLLSGTDVDSLSCDSTSSATSPTSMPCLVAGHHMWASKSGHHMLGLIEDYDALYKQISWGQTLLAKM DVQTQEALSPTSHKLGPKGSSSVPLSKFLSSMNTAKLVLEKASRLLKLFWRVSVPTNGQCSLHCEQIGEM KAENTKLHKKLFEQEKKLQNTAKLLQQSKHQEKVIFDQLVITHQVLRKARGNLELRPGATRPGASSPSRP GS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_145990 |
ORF Size | 5466 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145990.4, NP_666102.2 |
RefSeq Size | 11840 bp |
RefSeq ORF | 5469 bp |
Locus ID | 214444 |
UniProt ID | Q8K389 |
MW | 205.9 kDa |
Gene Summary | Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion (PubMed:20627074). Involved in regulation of mitotic spindle orientation (PubMed:20460369). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Required for the recruitment of AKAP9 to centrosomes (By similarity). Plays a role in neurogenesis (PubMed:20471352). Contrary to higher mammalian orthologs, including human, chimpanzee, bovine and dog, does not interact with EB1/MAPRE1, therefore its function in the regulation of microtubule dynamics is unclear (PubMed:19553473).[UniProtKB/Swiss-Prot Function] |
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