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Gtf2i (NM_001080747) Mouse Tagged ORF Clone
CAT＃: MR226075
- TrueORF®
Gtf2i (Myc-DDK-tagged) - Mouse general transcription factor II I (Gtf2i), transcript variant 3
ORF Plasmid: tGFP
"NM_001080747" in other vectors (3)
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CNY 9,790.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6030441I21Rik; BAP-135; Diws1t; GtfII-I; Spin; TFII-I; WBSCR6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226075 representing NM_001080747
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAAGTAGTGATGTCTGCCTTGCCTGCCGAAGATGAAGAGTCTTCAGAGAGCAGGATGGTGGTGA CCTTCCTCATGTCTGCCCTGGAGTCCATGTGTAAAGAGCTGGCCAAGTCCAAGGCTGAGGTGGCCTGCAT TGCTGTGTATGAGACCGACGTGTTTGTTGTTGGAACCGAAAGAGGGCGTGCTTTTGTCAATACCAGAAAA GATTTCCAAAAAGACTTTGTGAAGTATTGTGTTGAAGAAGAAGAAAAGGCCGCCGAGATGCATAAGATGA AATCTACCACCCAGGCCAATCGGATGAGTGTGGATGCTGTAGAAATTGAAACACTCAGAAAAACGGTGGA GGACTATTTCTGTTTTTGCTATGGGAAAGCTTTAGGCAAATCCACAGTGGTCCCTGTGCCATATGAGAAG ATGCTGCGCGACCAGTCGGCTGTGGTGGTGCAGGGGCTTCCCGAGGGGGTTGCCTTCAAGCACCCCGAAC ATTACGACCTCGCAACCTTGAAGTGGATTTTGGAGAACAAGGCAGGGATTTCCTTCATCATTAAGAGACC TTTCCTCGAGCCGAAGAAACACCTCGGTGGTCGAGTGCTGGCGGCCGAGGCTGAGAGGTCCATGCTGTCT CCTAGTGGAAGTTGTGGCCCCATCAAAGTGAAAACTGAACCCACAGAAGATTCTGGCATTTCTCTGGAAA TGGCCGCTGTGACAGTGAAGGAGGAGTCAGAAGACCCTGATTACTATCAGTATAACATTCAAGGCCCTTC TGAAACTGATGGTGTTGATGAAAAGCTCCCCCTTTCGAAGGCTTTGCAAGGAAGCCATCACTCCTCAGAA GGCAACGAGGGAACGGAAGTGGAAGTGCCAGCAGAAGATGATGACTATTCTCCACCCACCAAGAGGCTAA AGAGCACGGAGCCGCCCCCGCCCCCGCCCGTCCCGGAGCCCGCCAACGCTGGCAAGCGCAAAGTGAGGGA GTTCAACTTTGAGAAATGGAACGCACGCATCACTGACCTACGGAAACAAGTTGAAGAGTTGTTCGAAAGA AAATATGCTCAAGCCATCAAAGCCAAAGGCCCGGTGACGATCCCGTACCCTCTTTTCCAGTCCCATGTTG AAGATCTTTACGTAGAAGGGCTTCCTGAAGGGATTCCTTTTAGAAGGCCGTCGACGTACGGCATTCCTCG CCTTGAGAGGATATTGCTGGCGAAGGAAAGGATCCGCTTCGTGATTAAGAAACATGAGCTTCTGAACTCA ACACGAGAAGATTTACAGCTTGATAAACCAGCCTCAGGAGTGAAAGAAGAGTGGTACGCTCGCATCACCA AACTGAGGAAGATGGTTGACCAGCTCTTCTGCAAGAAATTTGCCGAGGCCTTGGGAAGCACGGAAGCCAA GGCTGTACCGTACCAAAAATTTGAGGCCCATCCCAATGATCTCTATGTGGAAGGACTTCCAGAAAACATT CCTTTCCGAAGCCCCTCGTGGTATGGAATCCCAAGACTGGAAAAAATCATCCAAGTGGGCAATCGAATTA AATTTGTTATCAAGAGACCAGAGCTGCTCACTCACAGCACAACTGAAGTGACTCAGCCACGGACAAACAC ACCAGTCAAAGAAGATTGGAATGTCAGAATCACCAAGCTCCGAAAGCAAGTGGAAGAGATCTTTAATTTG AAGTTTGCTCAGGCTCTGGGCCTCACAGAGGCTGTGAAGGTGCCATATCCTGTGTTCGAATCCAACCCGG AGTTTCTGTATGTGGAAGGGCTCCCCGAAGGGATCCCCTTCCGAAGCCCTACCTGGTTTGGGATTCCACG CCTCGAGAGGATTGTCCGTGGCAGCAATAAGATCAAGTTTGTTGTTAAAAAGCCTGAGCTAGTTGTGTCC TATTTGCCTCCTGGGATGGCTAGCAAAATCAACACTAAAGCATTGCAGTCCCCAAAACGACCACGAAGCC CTGGGAGCAACTCCAAGGTTCCTGAAATTGAGGTCACTGTGGAAGGCCCCAACAACAGCAGTCCTCAGAC CTCTGCGGTTCGAACTCCTACCCAGACCAATGGTTCAAATGTTCCCTTCAAGCCTCGAGGGAGAGAGTTT TCCTTTGAGGCCTGGAATGCTAAGATCACAGACCTGAAGCAGAAAGTGGAGAACCTCTTCAATGAAAAGT GCGGGGAAGCGCTTGGCCTTAAGCAGGCTGTGAAAGTCCCGTTTGCGTTGTTCGAGTCCTTCCCAGAAGA CTTCTATGTGGAAGGCTTGCCCGAGGGGGTGCCCTTCCGAAGGCCGTCCACATTTGGCATTCCCAGGCTG GAGAAGATACTCCGGAACAAGGCCAAGATAAAGTTCATCATTAAAAAACCTGAGATGTTCGAGACGGCCA TCAAAGAGAGCACCTCTTCCAAAAGCCCTCCAAGAAAGATAAACTCATCACCCAACGTTAATACTACTGC ATCAGGTGTGGAAGACCTGAACATCATCCAGGTGACAATTCCAGATGACGATAATGAACGACTGTCCAAA GTTGAAAAGGCCAGGCAGCTGCGGGAGCAGGTCAACGACCTCTTCAGTCGGAAGTTTGGTGAAGCTATTG GGATGGGCTTCCCGGTGAAGGTCCCCTACAGGAAGATCACCATCAACCCTGGCTGCGTGGTGGTCGATGG CATGCCCCCCGGGGTGTCCTTCAAAGCCCCCAGTTACCTGGAGATCAGCTCCATGAGGAGGATCTTAGAC TCTGCGGAGTTTATCAAGTTCACTGTCATTAGACCATTTCCAGGACTTGTGATTAATAACCAGCTGGTTG ATCAGAACGAGTCTGAAGGCCCTGTGATACAAGAATCAGCCGAGGCAAGCCAGTTGGAAGTCCCAGTAAC AGAAGAAATTAAAGAGACAGATGGGAGTTCACAGATCAAGCAGGAGCCAGACCCCACGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226075 representing NM_001080747
Red=Cloning site Green=Tags(s) MAQVVMSALPAEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRK DFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEK MLRDQSAVVVQGLPEGVAFKHPEHYDLATLKWILENKAGISFIIKRPFLEPKKHLGGRVLAAEAERSMLS PSGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQGPSETDGVDEKLPLSKALQGSHHSSE GNEGTEVEVPAEDDDYSPPTKRLKSTEPPPPPPVPEPANAGKRKVREFNFEKWNARITDLRKQVEELFER KYAQAIKAKGPVTIPYPLFQSHVEDLYVEGLPEGIPFRRPSTYGIPRLERILLAKERIRFVIKKHELLNS TREDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAVPYQKFEAHPNDLYVEGLPENI PFRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRITKLRKQVEEIFNL KFAQALGLTEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSNKIKFVVKKPELVVS YLPPGMASKINTKALQSPKRPRSPGSNSKVPEIEVTVEGPNNSSPQTSAVRTPTQTNGSNVPFKPRGREF SFEAWNAKITDLKQKVENLFNEKCGEALGLKQAVKVPFALFESFPEDFYVEGLPEGVPFRRPSTFGIPRL EKILRNKAKIKFIIKKPEMFETAIKESTSSKSPPRKINSSPNVNTTASGVEDLNIIQVTIPDDDNERLSK VEKARQLREQVNDLFSRKFGEAIGMGFPVKVPYRKITINPGCVVVDGMPPGVSFKAPSYLEISSMRRILD SAEFIKFTVIRPFPGLVINNQLVDQNESEGPVIQESAEASQLEVPVTEEIKETDGSSQIKQEPDPTW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001080747 |
| ORF Size | 2931 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001080747.2 |
| RefSeq Size | 4392 bp |
| RefSeq ORF | 2934 bp |
| Locus ID | 14886 |
| UniProt ID | Q9ESZ8 |
| MW | 110.4 kDa |
| Gene Summary | Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box (By similarity). Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226075 | Gtf2i (tGFP-tagged) - Mouse general transcription factor II I (Gtf2i) transcript variant 3, (10ug) |
CNY 10,740.00 |
|
| MR226075L3 | Lenti ORF clone of Gtf2i (Myc-DDK-tagged) - Mouse general transcription factor II I (Gtf2i), transcript variant 3 |
CNY 11,690.00 |
|
| MR226075L4 | Lenti ORF clone of Gtf2i (mGFP-tagged) - Mouse general transcription factor II I (Gtf2i), transcript variant 3 |
CNY 11,690.00 |
