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Msh5 (NM_001146215) Mouse Tagged ORF Clone
CAT＃: MR226215
- TrueORF®
Msh5 (Myc-DDK-tagged) - Mouse mutS homolog 5 (E. coli) (Msh5), transcript variant 2
ORF Plasmid: tGFP
"NM_001146215" in other vectors (4)
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CNY 6,112.00
CNY 7,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Mut5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226215 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTCAGAGCGACCCCAGGCCGGACGCCGCCGGGACCCGGACCCAGATCCGGGATCCCCTCAGCCA GCTTCCCCAGCCCTCAGCCCCCAATGGCGGGGCCTGGAGGTATCGAGGAAGAGGACGAGGAGGAGCCCGC TGAGATCCATCTGTGCGTGCTGTGGAGCTCGGGATACCTGGGCATTGCTTACTATGACACTAGTGACTCC ACTATCCACTTCATGCCAGATGCCCCAGACCACGAGAGCCTAAAGCTTCTCCAGAGAGTTCTGGATGAAA TCAACCCCCAGTCTGTTGTCACAAGTGCCAAACAGGATGAGGCTATGACTCGATTTCTAGGGAAGCTTGC CTCCGAGGAGCACAGAGAGCCAAAGGGACCTGAAATCATACTTCTGCCAAGCGTGGATTTTGGTCCAGAG ATAAGCAAACAGCGTCTCCTTTCCGGAAACTACTCCTTCATCTCAGACTCCATGACTGCTACTGAGAAAA TCCTTTTCCTCTCCTCCATTATTCCCTTTGACTGTGTCCTCACGGTCCGGGCACTTGGAGGACTGCTCAA GTTCCTGAGTCGAAGAAGAATTGGGGTTGAACTGGAAGACTATGATGTTGGCGTCCCTATCCTGGGATTC AAGAAGTTTGTATTGACCCATCTGGTGAGCATAGATCAAGACACTTACAGCGTTCTACAGATTTTCAAGA GTGAGTCTCACCCCTCGGTGTACAAAGTAGCCAGTGGGCTGAAGGAGGGGCTCAGCCTTTTTGGAATCCT CAACAGATGCCGCTGTAAGTGGGGACAGAAGCTGCTCAGGCTGTGGTTTACACGTCCAACCCGGGAGCTA AGGGAACTCAATTCCCGACTGGATGTCATTCAGTTCTTCCTGATGCCTCAGAACCTGGACATGGCCCAGA TGCTGCACCGACTCCTGAGCCACATCAAGAATGTGCCTCTGATTCTGAAACGCATGAAGTTGTCCCACAC CAAGGTCAGTGACTGGCAGGTCCTCTACAAGACTGTGTACAGTGCTCTCGGCCTGAGGGATGCCTGCCGT TCTCTGCCACAGTCCATCCAGCTTTTTCAGGACATTGCCCAGGAGTTCTCTGACGACCTGCATCACATTG CCAGCCTCATCGGGAAGGTGGTGGACTTTGAGGAAAGTCTTGCTGAAAATCGCTTCACAGTCCTCCCTAA CATAGACCCTGACATAGATGCCAAGAAGCGAAGGCTGATAGGGCTTCCGAGCTTCCTCACTGAAGTTGCT CAGAAGGAGCTGGAGAACCTGGACTCTCGCATCCCCTCATGCAGTGTCATCTACATCCCTCTGATTGGCT TCCTTCTTTCCATTCCCCGCTTGCCTTTCATGGTGGAAGCTAGTGACTTTGAGATTGAGGGGCTGGACTT CATGTTTCTCTCAGAGGACAAGCTGCACTATCGTAGCGCCCGGACCAAGGAGCTGGACACGCTGCTGGGA GACCTGCACTGCGAGATCCGGGACCAGGAGACCCTGTTGATGTACCAGCTGCAGTGCCAGGTGCTGGCAC GGGCTTCGGTCTTGACTCGGGTATTGGACCTTGCCTCCCGCCTGGACGTCCTGTTGGCTCTTGCCAGTGC TGCCCGGGACTACGGCTATTCGAGACCGCATTACTCTCCCTGTATCCATGGAGTACGAATCAGGAATGGC AGGCATCCTCTGGTGGAACTGTGTGCACGAACCTTCGTGCCCAACTCCACGGACTGTGGTGGGGACCAGG GCAGGGTCAAAGTCATCACTGGGCCCAACTCCTCAGGGAAAAGCATATATCTCAAGCAGGTAGGCTTGAT CACTTTCATGGCCCTGGTGGGCAGTTTCGTGCCTGCAGAGGAGGCCGAGATTGGGGTAATCGACGCCATC TTCACTCGAATTCACAGCTGCGAATCCATCTCCCTCGGCCTCTCCACCTTCATGATTGATCTCAACCAGG TGGCGAAAGCAGTGAACAATGCCACAGAGCACTCGCTGGTCCTGATCGATGAATTCGGGAAGGGGACCAA CTCGGTGGATGGCCTGGCACTTCTGGCTGCTGTGCTCCGTCACTGGCTAGCACTGGGACCCAGCTGCCCC CACGTCTTTGTAGCCACCAACTTCCTGAGCCTTGTTCAGCTGCAGCTGCTGCCGCAAGGACCCCTGGTGC AGTATTTGACCATGGAGACTTGTGAGGATGGGGAAGACCTTGTCTTCTTCTACCAGCTTTGCCAAGGCGT CGCCAGTGCCAGCCACGCCTCCCACACAGCGGCCCAGGCTGGGCTTCCTGACCCACTCATTGCTCGTGGC AAAGAGGTCTCAGACTTGATCCGCAGTGGGAAACCCATCAAGGCCACGAATGAGCTTCTAAGGAGAAACC AAATGGAAAACTGCCAGGCACTGGTGGATAAGTTTCTAAAACTGGACTTGGAGGATCCCACCCTGGACCT GGACATTTTCATTAGTCAGGAAGTGCTGCCCGCTGCTCCCACCATCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226215 protein sequence
Red=Cloning site Green=Tags(s) MAFRATPGRTPPGPGPRSGIPSASFPSPQPPMAGPGGIEEEDEEEPAEIHLCVLWSSGYLGIAYYDTSDS TIHFMPDAPDHESLKLLQRVLDEINPQSVVTSAKQDEAMTRFLGKLASEEHREPKGPEIILLPSVDFGPE ISKQRLLSGNYSFISDSMTATEKILFLSSIIPFDCVLTVRALGGLLKFLSRRRIGVELEDYDVGVPILGF KKFVLTHLVSIDQDTYSVLQIFKSESHPSVYKVASGLKEGLSLFGILNRCRCKWGQKLLRLWFTRPTREL RELNSRLDVIQFFLMPQNLDMAQMLHRLLSHIKNVPLILKRMKLSHTKVSDWQVLYKTVYSALGLRDACR SLPQSIQLFQDIAQEFSDDLHHIASLIGKVVDFEESLAENRFTVLPNIDPDIDAKKRRLIGLPSFLTEVA QKELENLDSRIPSCSVIYIPLIGFLLSIPRLPFMVEASDFEIEGLDFMFLSEDKLHYRSARTKELDTLLG DLHCEIRDQETLLMYQLQCQVLARASVLTRVLDLASRLDVLLALASAARDYGYSRPHYSPCIHGVRIRNG RHPLVELCARTFVPNSTDCGGDQGRVKVITGPNSSGKSIYLKQVGLITFMALVGSFVPAEEAEIGVIDAI FTRIHSCESISLGLSTFMIDLNQVAKAVNNATEHSLVLIDEFGKGTNSVDGLALLAAVLRHWLALGPSCP HVFVATNFLSLVQLQLLPQGPLVQYLTMETCEDGEDLVFFYQLCQGVASASHASHTAAQAGLPDPLIARG KEVSDLIRSGKPIKATNELLRRNQMENCQALVDKFLKLDLEDPTLDLDIFISQEVLPAAPTIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001146215 |
| ORF Size | 2502 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146215.1, NM_001146215.2, NP_001139687.1 |
| RefSeq Size | 2693 bp |
| RefSeq ORF | 2502 bp |
| Locus ID | 17687 |
| UniProt ID | Q9QUM7 |
| MW | 92.6 kDa |
| Gene Summary | This gene encodes a member of the MutS family of proteins that play critical roles in DNA mismatch repair and meiotic homologous recombination processes. Mice lacking the encoded protein are viable but sterile, with severe defects in spermatogenesis in males and complete loss of ovarian structures in females. Mutations in a similar gene in humans have been shown to cause common variable immune deficiency (CVID) and immunoglobulin A deficiency. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221932 | Msh5 (untagged) - Mouse mutS homolog 5 (E. coli) (Msh5), transcript variant 2, (10ug) |
CNY 10,640.00 |
|
| MG226215 | Msh5 (tGFP-tagged) - Mouse mutS homolog 5 (E. coli) (Msh5) transcript variant 2, (10ug) |
CNY 8,080.00 |
|
| MR226215L3 | Lenti ORF clone of Msh5 (Myc-DDK-tagged) - Mouse mutS homolog 5 (E. coli) (Msh5), transcript variant 2 |
CNY 9,220.00 |
|
| MR226215L4 | Lenti ORF clone of Msh5 (mGFP-tagged) - Mouse mutS homolog 5 (E. coli) (Msh5), transcript variant 2 |
CNY 9,220.00 |
