Capn3 (NM_001109761) Mouse Tagged ORF Clone
CAT#: MR226222
- TrueORF®
Capn3 (Myc-DDK-tagged) - Mouse calpain 3 (Capn3), transcript variant b
ORF Plasmid: tGFP
"NM_001109761" in other vectors (4)
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CNY 7,030.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI323605; Capa-3; Capa3; Lp82; p94 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226222 representing NM_001109761
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTACCTGCTGCCGGGATTCTTCTGTGACAGGGTGATCCGAGAAAGGGACAGGAGAAATGGAGAGG GCACTGTCTCACAACCTCTCAAGTTTGAGGGGCAGGATTTTGTCGTTCTCAAACAACAGTGTCTGGCTCA GAAGTGCCTCTTTGAAGACCGTGTCTTCCCAGCAGGTGTGCAGGCCCTTGGCTCCCATGAGCTGAGCCAG AAAGCCAAGATGAAGGCCATCACTTGGAAGAGGCCAAAGGAAATTTGTGAGAATCCCCGGTTTATCATTG GTGGAGCCAACAGGACTGACATCTGCCAAGGAGATCTAGGGGACTGCTGGTTTCTTGCAGCCATCGCCTG CCTGACCCTGAATGAGCGGCTGCTTTTCCGAGTTATACCCCATGATCAAAGTTTCACTGAAAACTACGCA GGGATCTTCCACTTCCAGTTCTGGCGCTATGGAGACTGGGTAGATGTGGTCATTGACGACTGTCTGCCAA CATACAACAATCAGCTGGTTTTCACCAAATCCAACCACCGCAATGAGTTCTGGAGTGCTCTCCTTGAGAA GGCTTATGCCAAGCTCCATGGCTCCTATGAAGCTCTGAAAGGTGGGAATACCACAGAAGCCATGGAGGAC TTCACAGGAGGGGTGACAGAGTTTTTTGAGATCAAGGATGCTCCGAGTGACATGTACAAGATTATGAGGA AAGCTATCGAGAGAGGCTCCCTCATGGGCTGCTCCATTGACACAATTGTTCCAGTGCAGTATGAAACAAG AATGGCCTGTGGGTTGGTGAAAGGGCATGCCTATTCAGTCACTGGGCTGGAGGAGGCCCTGTTCAAAGGT GAGAAGGTGAAGTTGGTGCGGCTGCGGAACCCCTGGGGCCAGGTGGAGTGGAACGGCTCTTGGAGTGATG GTTGGAAGGACTGGAGCTTTGTAGACAAAGATGAGAAGGCCCGTCTACAGCACCAGGTTACTGAGGATGG AGAGTTCTGGATGTCATATGATGACTTCGTCTACCATTTCACGAAGCTGGAGATCTGCAACCTCACAGCT GACGCCCTGGAGTCCGATAAGCTTCAGACCTGGACGGTGTCTGTAAACGAGGGCCGCTGGGTGAGGGGCT GTTCTGCTGGAGGCTGCCGAAACTTCCCAGACACTTTCTGGACCAACCCGCAGTACCGTCTCAAGCTTCT GGAGGAAGACGATGACCCTGAGGACTCTGAGGTAATCTGCAGCTTCCTCGTGGCTCTGATGCAGAAGAAC CGGCGCAAGGACCGGAAGCTGGGGGCCAACCTCTTCACTATTGGCTTCGCCATCTACGAGGTTCCCAAAG AGATGCACGGGAATAAGCAACACCTGCAGAAGGACTTCTTCTTGTACAACGCCTCCAAGGCCAGAAGCAA AACCTACATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCACCCAGCGAGTATGTCATCGTACCC TCCACCTACGAGCCCCACCAGGAGGGGGAATTCATCCTCCGAGTCTTCTCCGAAAAGAGGAATCTCTCTG AGGAAGCTGAAAATACAATCTCTGTGGATCGGCCAGTGCCAAGGCCTGGCCACACAGACCAGGAAAGTGA GGAGCAGCAGCAATTCCGGAACATCTTCAGGCAGATCGCAGGCGACGACATGGAGATCTGTGCAGATGAA CTCAAGAATGTCCTCAACACAGTGGTGAACAAACACAAGGACCTGAAGACACAAGGGTTCACGCTGGAGT CCTGCAGAAGCATGATAGCTCTCATGGATACAGATGGCTCTGGAAGACTGAATCTTCAAGAGTTCCATCA CCTCTGGAAAAAGATCAAGGCCTGGCAGAAAATCTTCAAGCACTATGACACAGACCATTCCGGTACCATC AATAGCTATGAGATGCGAAATGCAGTCAATGATGCAGGCTTCCATCTCAACAGCCAACTCTATGACATCA TCACCATGCGCTATGCGGACAAACACATGAACATCGACTTTGACAGCTTCATCTGCTGCTTCGTCAGGCT GGAAGGGATGTTCAGAGCTTTTAACGCATTTGACAAGGATGGCGATGGTATCATCAAACTGAATGTACTT GAGTGGCTGCAGCTTACCATGTATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226222 representing NM_001109761
Red=Cloning site Green=Tags(s) MPYLLPGFFCDRVIRERDRRNGEGTVSQPLKFEGQDFVVLKQQCLAQKCLFEDRVFPAGVQALGSHELSQ KAKMKAITWKRPKEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYA GIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMED FTGGVTEFFEIKDAPSDMYKIMRKAIERGSLMGCSIDTIVPVQYETRMACGLVKGHAYSVTGLEEALFKG EKVKLVRLRNPWGQVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVYHFTKLEICNLTA DALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPEDSEVICSFLVALMQKN RRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVP STYEPHQEGEFILRVFSEKRNLSEEAENTISVDRPVPRPGHTDQESEEQQQFRNIFRQIAGDDMEICADE LKNVLNTVVNKHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSGTI NSYEMRNAVNDAGFHLNSQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFNAFDKDGDGIIKLNVL EWLQLTMYA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001109761 |
ORF Size | 2127 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001109761.2, NP_001103231.1 |
RefSeq Size | 2624 bp |
RefSeq ORF | 2130 bp |
Locus ID | 12335 |
MW | 82.7 kDa |
Gene Summary | Calcium-regulated non-lysosomal thiol-protease.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220707 | Capn3 (untagged) - Mouse calpain 3 (Capn3), transcript variant b, (10ug) |
CNY 9,120.00 |
|
MG226222 | Capn3 (tGFP-tagged) - Mouse calpain 3 (Capn3) transcript variant b, (10ug) |
CNY 9,904.00 |
|
MR226222L3 | Lenti ORF clone of Capn3 (Myc-DDK-tagged) - Mouse calpain 3 (Capn3), transcript variant b |
CNY 8,930.00 |
|
MR226222L4 | Lenti ORF clone of Capn3 (mGFP-tagged) - Mouse calpain 3 (Capn3), transcript variant b |
CNY 8,930.00 |