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Sh3kbp1 (NM_001135727) Mouse Tagged ORF Clone
CAT＃: MR226320
- TrueORF®
Sh3kbp1 (Myc-DDK-tagged) - Mouse SH3-domain kinase binding protein 1 (Sh3kbp1), transcript variant 1
ORF Plasmid: tGFP
"NM_001135727" in other vectors (4)
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CNY 4,952.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1200007H22Rik; 1700125L08Rik; 5830464D22Rik; AI447724; Cin85; IN85; Ruk; Seta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226320 representing NM_001135727
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGAGGCCATAGTGGAGTTTGATTACCAGGCCCAGCATGATGATGAGCTGACAATCAGCGTGGGTG AGGTCATCACCAACATCAGGAAGGAGGATGGAGGCTGGTGGGAGGGACAGATCAACGGCAGGAGAGGTTT GTTCCCTGACAACTTTGTAAGAGAAATAAAGAAAGATATGAAGAAAGATCTTCTCTCTAACAAAGCTCCA GAGAAACCCATGCACGATGTATCCAGTGGAAACGCTCTGCTTTCTTCTGAAACCATTTTAAGAACCAATA AACGAGGAGAACGACGGAGACGCCGATGCCAGGTGGCATTCAGCTACCTGCCCCAGAATGACGATGAGCT GGAGCTGAAAGTCGGGGACATCATAGAGGTTGTAGGAGAGGTAGAGGAAGGATGGTGGGAAGGTGTGCTC AATGGGAAGACTGGAATGTTTCCTTCCAACTTCATCAAGGAGCTGTCAGGGGAGTCGGATGAGCTTGGCA TCTCCCAGGATGAGCAGCTTTCCAAGTCAAGCTTAAGAGAAACCACAGGCTCCGAGAGTGATGGAGGCGA CTCCAGCAGTACCAAGTCCGAAGGTGCCAACGGGACCATGGCAACTGCAGCCATCCAGCCCAAGAAAGTT AAAGGAGTGGGGTTTGGAGACATTTTCAAAGACAAGCCAATTAAACTAAGACCAAGGTCGATTGAAGTGG AAAATGACTTCCTGCCCGTCGAAAAGACAATTGGGAAGAAGTTACCTCCAGCTACATCAACTCCAGACCC ATCGAAGACGGAGATGGACAGCAGGACAAAGACCAAGGATTACTGCAAAGTAATATTTCCATATGAGGCA CAGAATGATGATGAATTGACAATCAAGGAAGGAGATATAGTGACTCTCATCAATAAGGACTGCATCGACG TAGGCTGGTGGGAAGGAGAACTCAATGGCAGACGAGGCGTGTTTCCTGACAACTTCGTGAAGTTACTTCC ATCGGACTTTGACAAGGAAGGGAATAGACCCAAGAAACCGCCACCTCCATCAGCTCCTGTCGTCAAACAA GGGGCAGGTACCACAGAGAGAAAACATGAAATTAAAAAAATACCTCCTGAAAGACCAGAAACCCTTCCAA ACAGAACAGAAGAGAAAGAAAGACCAGAGAGAGAGCCAAAACTGGATTTACAGAAGCCTTCAGTTCCTGC CATCCCACCAAAAAAGCCTCGGCCACCTAAGACCAATTCTCTGAACAGACCTGGTGCATTGCCCCCAAGA AGACCTGAGCGACCAGTGGGCCCACTGACACACACCAGGGGTGACAGTCCAAAGATTGACTTGGCAGGCA GCGCACTCTCTGGCATCCTGGATAAGGACCTCTCAGACCGCAGCAATGATATTGACTTAGAAGGTTTTGA CTCTGTGATATCATCTACTGAGAAACTCAGCCACCCAACCACAAGCAGACCAAAAGCTACAGGAAGACGG CCTCCATCCCAGTCACTCACATCTTCTTCCCTTTCAAGCCCCGATATCTTTGACTCCCCAAGTCCCGAAG AAGATAAAGAGGAACACATTTCGCTTGCGCACAGAGGAATAGACGTGTCAAAGAAGACTTCCAAGACTGT TACCATATCCCAAGTGTCTGATAACAAGACATCCCTGCCACCCAAGCCAGGGACCATGGCAGCTGCCAGC AGTGGGCCAGCTTCTCTCTCTTCAGTGGCATCCTCACCCATGTCATCCTCTTTGGGAACAGCTGGACAGA GAGCCAGTTCTCCATCTCTGTTCAGCACAGAAGGAAAGCCAAAGATGGAGCCAGCAGTGAGCAGCCAGGC TGCTATCGAGGAGCTTAAGATGCAAGTCCGTGAGCTGAGGACCATCATTGAGACCATGAAGGACCAGCAG AAACGTGAGATTAAGCAGTTACTGTCAGAATTGGATGAAGAGAAAAAGATCCGGCTCCGGTTGCAGATGG AAGTGAACGACATAAAGAAAGCTCTTCAATCAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226320 representing NM_001135727
Red=Cloning site Green=Tags(s) MVEAIVEFDYQAQHDDELTISVGEVITNIRKEDGGWWEGQINGRRGLFPDNFVREIKKDMKKDLLSNKAP EKPMHDVSSGNALLSSETILRTNKRGERRRRRCQVAFSYLPQNDDELELKVGDIIEVVGEVEEGWWEGVL NGKTGMFPSNFIKELSGESDELGISQDEQLSKSSLRETTGSESDGGDSSSTKSEGANGTMATAAIQPKKV KGVGFGDIFKDKPIKLRPRSIEVENDFLPVEKTIGKKLPPATSTPDPSKTEMDSRTKTKDYCKVIFPYEA QNDDELTIKEGDIVTLINKDCIDVGWWEGELNGRRGVFPDNFVKLLPSDFDKEGNRPKKPPPPSAPVVKQ GAGTTERKHEIKKIPPERPETLPNRTEEKERPEREPKLDLQKPSVPAIPPKKPRPPKTNSLNRPGALPPR RPERPVGPLTHTRGDSPKIDLAGSALSGILDKDLSDRSNDIDLEGFDSVISSTEKLSHPTTSRPKATGRR PPSQSLTSSSLSSPDIFDSPSPEEDKEEHISLAHRGIDVSKKTSKTVTISQVSDNKTSLPPKPGTMAAAS SGPASLSSVASSPMSSSLGTAGQRASSPSLFSTEGKPKMEPAVSSQAAIEELKMQVRELRTIIETMKDQQ KREIKQLLSELDEEKKIRLRLQMEVNDIKKALQSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001135727 |
| ORF Size | 1995 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001135727.2, NP_001129199.1 |
| RefSeq Size | 5029 bp |
| RefSeq ORF | 1998 bp |
| Locus ID | 58194 |
| UniProt ID | Q8R550 |
| MW | 73.8 kDa |
| Gene Summary | Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through an association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may be inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit. May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220257 | Sh3kbp1 (untagged) - Mouse SH3-domain kinase binding protein 1 (Sh3kbp1), transcript variant 1, (10ug) |
CNY 8,550.00 |
|
| MG226320 | Sh3kbp1 (tGFP-tagged) - Mouse SH3-domain kinase binding protein 1 (Sh3kbp1) transcript variant 1, (10ug) |
CNY 6,370.00 |
|
| MR226320L3 | Lenti ORF clone of Sh3kbp1 (Myc-DDK-tagged) - Mouse SH3-domain kinase binding protein 1 (Sh3kbp1), transcript variant 1 |
CNY 7,700.00 |
|
| MR226320L4 | Lenti ORF clone of Sh3kbp1 (mGFP-tagged) - Mouse SH3-domain kinase binding protein 1 (Sh3kbp1), transcript variant 1 |
CNY 7,700.00 |
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