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Epha5 (NM_007937) Mouse Tagged ORF Clone
CAT＃: MR226341
- TrueORF®
Epha5 (Myc-DDK-tagged) - Mouse Eph receptor A5 (Epha5)
ORF Plasmid: tGFP
"NM_007937" in other vectors (4)
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CNY 6,416.00
CNY 7,700.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI854630; AW125296; bsk; Cek7; Ehk1; Els1; Hek7; Rek7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226341 representing NM_007937
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGCTCCGGGCCCCGCGGTGCGGGACACCGACGGACCCAGGGCAGAGGTGGCGGCGACGACACCC CCCGCGTCCCTGCCTCTCTGGCAGGCTGCTATTCGGCACCTCTAAAGGGCCCCCTCTGGACGTGCCTTCT CTTGTGTGCGGCGCTCCGGACCCTTTTGGCCAGCCCCAGCAACGAAGTGAATTTGTTGGATTCGCGCACT GTCATGGGGGACCTTGGATGGATTGCTTTTCCAAAGAACGGGTGGGAAGAGATTGGTGAAGTTGATGAGA ACTATGCCCCCATCCACACATACCAAGTGTGCAAAGTTATGGAACAGAATCAGAATAATTGGCTGTTGAC CAGTTGGATCTCTAACGAAGGTGCTTCCAGAATCTTTATTGAACTCAAGTTTACTTTAAGGGACTGCAAC AGCCTTCCTGGAGGACTGGGGACTTGTAAGGAGACATTTAACATGTATTATTTTGAATCAGATGATGAGA ATGGGAGAAGTATCAAAGAGAACCAATACATCAAGATTGATACCATCGCTGCAGATGAGAGCTTCACAGA ACTTGATCTTGGTGACCGTGTCATGAAACTGAATACAGAGGTCAGAGATGTCGGACCTCTGAGCAAAAAG GGATTTTATCTTGCTTTCCAAGATGTCGGTGCTTGCATTGCTCTGGTTTCTGTCCGTGTCTACTATAAAA AGTGTCCCTCTGTAGTAAGACACTTGGCTATCTTCCCTGACACTATCACTGGAGCAGATTCATCACAGTT GTTAGAGGTGTCAGGCTCCTGCGTCAACCATTCTGTGACAGATGATCCTCCCAAGATGCATTGCAGTGCT GAAGGGGAGTGGCTGGTTCCCATTGGGAAATGCATGTGCAAGGCTGGATATGAAGAGAAAAATGGTACCT GCCAAGCTCCTTCTCCAGTCACCAATGTGAAAAAGGGGAAGATTGCAAAGAACAGCATTTCTTTGTCTTG GCAAGAGCCAGATCGCCCCAATGGAATTATCCTGGAGTATGAAATCAAGTACTTTGAAAAGGACCAAGAG ACCAGTTACACAATTATCAAGTCTAAAGAGACCAGTATTACAGCCGAGGGCCTGAAACCTGCATCTGTGT ATGTCTTCCAAATTCGAGCACGTACAGCAGCAGGCTACGGCGTCTTCAGTCGAAGATTTGAGTTTGAAAC CACACCAGTGTCAGTTGCAGCATCTAATGATCAAAGCCAGATTCCCATCATTGCAGTGTCAGTGACAGTG GGAGTCATCTTGTTGGCAGTGATGATCGGCTTCCTCCTCAGTGGCAGTTGCTGCGATTGTGGCTGTGGGA GGGCTTCTTCCCTGTGCGCTGTTGCCCATCCAAGCCTAATATGGCGGTGTGGCTACAGCAAAGCAAAGCA GGATCCAGAAGAGGAAAAGATGCACTTTCATAACGGGCACATTAAACTGCCAGGAGTCAGAACCTATATT GATCCGCACACTTATGAAGATCCCAATCAAGCTGTTCATGAATTTGCGAAGGAGATTGAAGCTTCATGCA TCACCATTGAGAGAGTGATCGGAGCAGGTGAATTTGGTGAAGTTTGCAGTGGACGTTTGAAACTACCTGG AAAAAGAGAATTACCTGTGGCTATCAAAACTCTTAAAGTAGGCTATACTGAAAAGCAGCGCAGAGATTTC CTGGGTGAAGCAAGTATTATGGGGCAGTTCGATCATCCAAACATCATCCATCTAGAAGGTGTTGTGACTA AAAGCAAACCTGTGATGATAGTGACAGAGTACATGGAGAACGGCTCCTTAGACACGTTTTTAAAGAAAAA CGATGGGCAGTTCACTGTGATTCAGCTTGTTGGCATGCTGAGAGGCATCGCTGCAGGAATGAAGTACCTT TCTGACATGGGCTACGTGCATAGAGACCTTGCTGCTAGAAACATCTTAATCAACAGTAACCTTGTGTGCA AGGTGTCTGACTTTGGACTTTCCAGGGTACTGGAAGATGATCCTGAGGCAGCCTACACCACAAGGGGAGG CAAAATTCCAATCAGATGGACTGCCCCGGAGGCAATAGCTTTTCGAAAGTTCACCTCTTCCAGTGATGTC TGGAGCTATGGCATTGTAATGTGGGAAGTTGTATCTTATGGAGAGAGACCCTACTGGGAGATGACCAATC AGGATGTGATCAAGGCAGTAGAAGAAGGCTACCGCCTGCCAAGCCCCATGGATTGCCCTGCTGCTCTCTA TCAATTAATGCTGGATTGCTGGCAGAAAGATCGAAACAGCAGGCCCAAGTTTGATGAAATCGTCAACATG CTGGACAAACTGATACGAAACCCAAGTAGTCTGAAGACACTGGTGAATGCGTCGAGCAGAGTGTCTACAT TGTTGGCAGAACATGGTTCTTTGGGGTCTGGGGCCTACAGATCAGTAGGTGAATGGCTGGAAGCAATCAA AATGGGTCGGTACACAGAGATTTTCATGGAAAATGGATACAGTTCAATGGACGCTGTGGCTCAGGTGACC TTGGAGGATTTGAGGCGCCTGGGAGTGACTCTGGTCGGTCACCAGAAGAAGATCATGAGCAGCCTTCAAG AGATGAAGGTGCAGATGGTAAACGGGATGGTGCCAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226341 representing NM_007937
Red=Cloning site Green=Tags(s) MRGSGPRGAGHRRTQGRGGGDDTPRVPASLAGCYSAPLKGPLWTCLLLCAALRTLLASPSNEVNLLDSRT VMGDLGWIAFPKNGWEEIGEVDENYAPIHTYQVCKVMEQNQNNWLLTSWISNEGASRIFIELKFTLRDCN SLPGGLGTCKETFNMYYFESDDENGRSIKENQYIKIDTIAADESFTELDLGDRVMKLNTEVRDVGPLSKK GFYLAFQDVGACIALVSVRVYYKKCPSVVRHLAIFPDTITGADSSQLLEVSGSCVNHSVTDDPPKMHCSA EGEWLVPIGKCMCKAGYEEKNGTCQAPSPVTNVKKGKIAKNSISLSWQEPDRPNGIILEYEIKYFEKDQE TSYTIIKSKETSITAEGLKPASVYVFQIRARTAAGYGVFSRRFEFETTPVSVAASNDQSQIPIIAVSVTV GVILLAVMIGFLLSGSCCDCGCGRASSLCAVAHPSLIWRCGYSKAKQDPEEEKMHFHNGHIKLPGVRTYI DPHTYEDPNQAVHEFAKEIEASCITIERVIGAGEFGEVCSGRLKLPGKRELPVAIKTLKVGYTEKQRRDF LGEASIMGQFDHPNIIHLEGVVTKSKPVMIVTEYMENGSLDTFLKKNDGQFTVIQLVGMLRGIAAGMKYL SDMGYVHRDLAARNILINSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAFRKFTSSSDV WSYGIVMWEVVSYGERPYWEMTNQDVIKAVEEGYRLPSPMDCPAALYQLMLDCWQKDRNSRPKFDEIVNM LDKLIRNPSSLKTLVNASSRVSTLLAEHGSLGSGAYRSVGEWLEAIKMGRYTEIFMENGYSSMDAVAQVT LEDLRRLGVTLVGHQKKIMSSLQEMKVQMVNGMVPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007937 |
| ORF Size | 2628 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007937.3, NP_031963.2 |
| RefSeq Size | 7789 bp |
| RefSeq ORF | 2631 bp |
| Locus ID | 13839 |
| UniProt ID | Q60629 |
| MW | 97.5 kDa |
| Gene Summary | Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222308 | Epha5 (untagged) - Mouse Eph receptor A5 (Epha5), (10ug) |
CNY 11,210.00 |
|
| MG226341 | Epha5 (tGFP-tagged) - Mouse Eph receptor A5 (Epha5), (10ug) |
CNY 8,016.00 |
|
| MR226341L3 | Lenti ORF clone of Epha5 (Myc-DDK-tagged) - Mouse Eph receptor A5 (Epha5) |
CNY 9,600.00 |
|
| MR226341L4 | Lenti ORF clone of Epha5 (mGFP-tagged) - Mouse Eph receptor A5 (Epha5) |
CNY 9,600.00 |
