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Rps6ka3 (NM_148945) Mouse Tagged ORF Clone
CAT＃: MR226694
- TrueORF®
Rps6ka3 (Myc-DDK-tagged) - Mouse ribosomal protein S6 kinase polypeptide 3 (Rps6ka3)
ORF Plasmid: tGFP
"NM_148945" in other vectors (4)
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CNY 5,424.00
CNY 6,460.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MAPKAPK-1b; MPK-9; p90RSK3; pp90RSK2; Rsk2; S6K-alpha3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226694 representing NM_148945
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCTGGCGCAGCTGGCGGACCCGTGGCAGAAGATGGCTGTGGAGAGCCCTTCCGACAGCGCGGAGA ATGGACAGCAAATTATGGATGAACCTATGGGAGAGGAGGAGATTAACCCACAAACTGAAGAAGGCAGTAT CAAAGAAATTGCAATCACACATCATGTGAAGGAAGGACATGAAAAGGCAGATCCTTCCCAGTTTGAACTT TTAAAAGTATTAGGGCAGGGATCATTTGGAAAGGTTTTCTTAGTTAAAAAAATCTCAGGCTCTGATGCTA GACAGCTTTATGCCATGAAAGTATTAAAGAAGGCCACGCTGAAAGTTCGAGACCGTGTTCGGACAAAAAT GGAACGTGATATCTTGGTAGAAGTCAATCACCCTTTCATTGTCAAATTGCATTACGCTTTTCAAACGGAA GGGAAGTTGTATCTTATTTTGGATTTTCTCAGGGGCGGAGACTTGTTTACACGCTTATCCAAAGAGGTGA TGTTCACAGAGGAAGATGTCAAATTCTACTTGGCTGAACTTGCACTTGCTTTAGACCATCTTCATAGCCT GGGAATAATCTATAGAGACTTAAAACCAGAAAACATACTTCTTGATGAAGAAGGTCACATCAAGTTAACT GATTTTGGCTTAAGTAAGGAATCTATTGATCATGAGAAGAAGGCTTATTCTTTTTGTGGCACTGTGGAAT ACATGGCTCCAGAAGTAGTTAACCGCAGAGGTCACACTCAGAGTGCGGACTGGTGGTCCTTTGGTGTGTT GATGTTTGAAATGCTCACTGGTACACTACCTTTCCAAGGAAAAGATCGTAAAGAAACAATGACTATGATT CTTAAAGCCAAACTCGGGATGCCACAGTTTCTGAGTCCTGAAGCCCAGAGTCTTTTACGAATGCTTTTCA AACGGAATCCTGCAAACAGATTAGGTGCTGGACCAGATGGAGTTGAAGAAATTAAAAGACATTCATTTTT CTCAACAATAGACTGGAATAAACTATATAGAAGAGAGATTCACCCACCTTTTAAGCCTGCAACTGGCAGA CCTGAAGATACATTTTATTTTGATCCTGAGTTTACTGCAAAAACTCCCAAAGATTCACCGGGCATTCCAC CTAGTGCTAACGCACATCAGCTTTTTCGGGGGTTTAGTTTTGTTGCTATTACCTCAGATGATGAAAGCCA AGCTATGCAGACAGTTGGTGTGCATTCAATTGTTCAGCAATTACACAGAAACAGTATTCAGTTTACTGAT GGATATGAAGTAAAAGAGGATATTGGCGTTGGCTCATACTCCGTTTGTAAGAGATGTATACATAAAGCTA CAAACATGGAGTTTGCCGTGAAGATTATTGATAAAAGCAAGAGAGACCCAACAGAAGAGATTGAAATTCT TCTTCGCTATGGACAGCATCCAAACATCATTACCCTAAAGGATGTGTATGATGATGGAAAATATGTGTAT GTAGTAACAGAACTTATGAAAGGAGGTGAATTGCTGGATAAGATTCTTAGACAGAAGTTTTTCTCAGAGC GAGAGGCCAGCGCTGTCCTGTTTACTATAACCAAAACTGTTGAGTATCTTCATGCACAAGGGGTGGTTCA CAGAGACTTGAAACCTAGCAACATTCTTTATGTGGATGAGTCTGGTAATCCAGAATCTATTCGAATTTGT GATTTTGGCTTTGCAAAACAACTGAGAGCAGAAAATGGTCTTCTCATGACTCCTTGTTATACTGCAAATT TTGTTGCACCAGAGGTTTTAAAACGGCAAGGTTATGATGCTGCCTGTGATATATGGAGTCTTGGCGTCCT CCTTTATACAATGCTTACTGGTTACACTCCATTTGCAAATGGCCCTGATGATACTCCAGAGGAAATACTG GCACGAATAGGTAGTGGAAAATTCTCACTCAGTGGTGGTTACTGGAATTCTGTTTCAGACACAGCAAAGG ACCTGGTGTCAAAGATGCTTCATGTAGATCCTCATCAGAGACTGACGGCTGCTCTGGTGCTCAGACATCC TTGGATTGTCCACTGGGACCAACTACCACAATACCAACTAAACAGACAGGATGCGCCGCATCTCGTAAAG GGTGCCATGGCAGCTACGTACTCTGCTTTAAACCGCAATCAGTCCCCAGTCTTGGAACCAGTGGGCCGCT CCACTCTTGCTCAGCGGAGAGGGATTAAAAAAATCACCTCAACAGCCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226694 representing NM_148945
Red=Cloning site Green=Tags(s) MPLAQLADPWQKMAVESPSDSAENGQQIMDEPMGEEEINPQTEEGSIKEIAITHHVKEGHEKADPSQFEL LKVLGQGSFGKVFLVKKISGSDARQLYAMKVLKKATLKVRDRVRTKMERDILVEVNHPFIVKLHYAFQTE GKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENILLDEEGHIKLT DFGLSKESIDHEKKAYSFCGTVEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGTLPFQGKDRKETMTMI LKAKLGMPQFLSPEAQSLLRMLFKRNPANRLGAGPDGVEEIKRHSFFSTIDWNKLYRREIHPPFKPATGR PEDTFYFDPEFTAKTPKDSPGIPPSANAHQLFRGFSFVAITSDDESQAMQTVGVHSIVQQLHRNSIQFTD GYEVKEDIGVGSYSVCKRCIHKATNMEFAVKIIDKSKRDPTEEIEILLRYGQHPNIITLKDVYDDGKYVY VVTELMKGGELLDKILRQKFFSEREASAVLFTITKTVEYLHAQGVVHRDLKPSNILYVDESGNPESIRIC DFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDAACDIWSLGVLLYTMLTGYTPFANGPDDTPEEIL ARIGSGKFSLSGGYWNSVSDTAKDLVSKMLHVDPHQRLTAALVLRHPWIVHWDQLPQYQLNRQDAPHLVK GAMAATYSALNRNQSPVLEPVGRSTLAQRRGIKKITSTAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_148945 |
| ORF Size | 2220 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_148945.2, NP_683747.1 |
| RefSeq Size | 2223 bp |
| RefSeq ORF | 2223 bp |
| Locus ID | 110651 |
| UniProt ID | P18654 |
| MW | 84.1 kDa |
| Gene Summary | Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. In LPS-stimulated dendritic cells, is involved in TLR4-induced macropinocytosis, and in myeloma cells, acts as effector of FGFR3-mediated transformation signaling, after direct phosphorylation at Tyr-529 by FGFR3. Phosphorylates DAPK1 (By similarity). Negatively regulates EGF-induced MAPK1/3 phosphorylation via phosphorylation of SOS1. Phosphorylates SOS1 at 'Ser-1134' and 'Ser-1161' that create YWHAB and YWHAE binding sites and which contribute to the negative regulation of MAPK1/3 phosphorylation (PubMed:22827337). Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221249 | Rps6ka3 (untagged) - Mouse ribosomal protein S6 kinase polypeptide 3 (Rps6ka3), (10ug) |
CNY 9,600.00 |
|
| MG226694 | Rps6ka3 (tGFP-tagged) - Mouse ribosomal protein S6 kinase polypeptide 3 (Rps6ka3), (10ug) |
CNY 7,130.00 |
|
| MR226694L3 | Lenti ORF clone of Rps6ka3 (Myc-DDK-tagged) - Mouse ribosomal protein S6 kinase polypeptide 3 (Rps6ka3) |
CNY 8,360.00 |
|
| MR226694L4 | Lenti ORF clone of Rps6ka3 (mGFP-tagged) - Mouse ribosomal protein S6 kinase polypeptide 3 (Rps6ka3) |
CNY 8,360.00 |
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