United States
Germany
Japan
United Kingdom
China
Eps15 (NM_001159964) Mouse Tagged ORF Clone
CAT＃: MR226696
- TrueORF®
Eps15 (Myc-DDK-tagged) - Mouse epidermal growth factor receptor pathway substrate 15 (Eps15), transcript variant 2
ORF Plasmid: tGFP
"NM_001159964" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,512.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2410112D09Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226696 representing NM_001159964
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATTTAAAAAGCGATTCAGGATTAGGAGGATGGATAGCCATACCAGCTGTAGCTGATGTTCTCAGAT ACTCTTGCATTGTTTGCTGGTCTTCTAGGGAAAAGAACAATGTGGAGCAGGACCTTAAAGAGAAGGAAGA CACAGTTAAGCAGAGGACCAGTGAGGTTCAGGATCTTCAAGATGAAGTTCAAAGGGAGAGTATTAATCTA CAAAAACTGCAGGCCCAGAAGCAGCAGGTGCAGGAGCTCCTGGGTGAACTGGATGAGCAGAAAGCCCAGC TGGAGGAGCAGCTCCAGGAAGTCAGGAAAAAGTGTGCTGAGGAGGCCCAGCTGATTTCTTCCCTGAAAGC AGAAATAACTAGTCAAGAATCTCAGATCTCCAGTTATGAGGAAGAACTGTTGAAAGCTAGAGAAGAACTA AGTCGCCTACAACAAGAAACAGCACAATTGGAAGAAAGTGTGGAGTCAGGGAAGGCTCAGCTGGAACCTC TTCAGCAGCACCTACAAGAGTCACAACAGGAAATCAGCTCAATGCAAATGAGATTGGAAATGAAAGATCT GGAAACTGATAATAACCAATCAAATTGGAGCAGTAGCCCACAAAGCGTTCTTGTTAATGGTGCTACAGAT TACTGTAGCCTCAGCACCAGCAGCAGTGAAACAGCCAACTTCAACGAACATGCTGAAGGCCAAAACAACC TAGAGTCTGAACCCACACACCAGGAGTCCTCAGTAAGAAGTAGTCCTGAAATCGCACCTTCTGATGTGAC TGATGAAAGTGAGGCTGTGACTGTGGCTGGTAATGAGAAAGTTACTCCGAGATTTGACGATGACAAGCAC TCAAAAGAGGAAGATCCATTTAATGTAGAATCAAGTTCACTGACAGATGCAGTTGCAGATACAAACTTGG ATTTTTTCCAGTCTGATCCTTTTGTTGGCAGTGATCCTTTCAAGGATGATCCTTTTGGAAAAATTGATCC ATTTGGTGGTGACCCTTTCAAAGGCTCAGATCCTTTTGCGTCTGATTGCTTCTTTAAGCAGACTTCTACT GATCCTTTTACCACTTCAAGTACGGACCCTTTCAGTGCATCCAGCAACAGCAGTAATACATCGGTAGAAA CTTGGAAGCACAATGACCCATTTGCTCCTGGTGGAACAGTTGTTGCTGCAGCGAGTGATTCAGCCACAGA CCCTTTTGCTTCTGTTTTCGGAAATGAATCATTTGGAGATGGATTTGCTGACTTCAGCACATTATCAAAG GTCAACAATGAAGATGCTTTTAATCCTACCATATCAAGTTCTACCAGCAGTGTGACCATTGCAAAACCTA TGTTAGAGGAAACAGCCAGCAAGAGTGAAGATGTGCCTCCAGCACTGCCGCCCAAAGTTGGCACTCCAAC AAGACCTTGCCCGCCACCCCCTGGGAAAAGACCCATCAACAAATTGGATTCTTCTGATCCCCTTAAACTG AATGATCCATTTCAGCCTTTCCCAGGCAATGATAGTCCCAAAGAAAAAGATCCTGATATGTTTTGTGATC CATTCACTTCTTCTACCACTACCAATAAAGAGGCTGACCCAAGCAATTTTGCTAACTTCAGTGCTTATCC CTCTGAAGAAGATATGATTGAATGGGCAAAAAGGGAAAGTGAGCGGGAAGAAGAACAGAGGCTTGCCAGA CTAAATCAGCAGGAGCAAGAAGACTTGGAACTGGCCATTGCACTTAGCAAATCTGAGATCTCAGAAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226696 representing NM_001159964
Red=Cloning site Green=Tags(s) MYLKSDSGLGGWIAIPAVADVLRYSCIVCWSSREKNNVEQDLKEKEDTVKQRTSEVQDLQDEVQRESINL QKLQAQKQQVQELLGELDEQKAQLEEQLQEVRKKCAEEAQLISSLKAEITSQESQISSYEEELLKAREEL SRLQQETAQLEESVESGKAQLEPLQQHLQESQQEISSMQMRLEMKDLETDNNQSNWSSSPQSVLVNGATD YCSLSTSSSETANFNEHAEGQNNLESEPTHQESSVRSSPEIAPSDVTDESEAVTVAGNEKVTPRFDDDKH SKEEDPFNVESSSLTDAVADTNLDFFQSDPFVGSDPFKDDPFGKIDPFGGDPFKGSDPFASDCFFKQTST DPFTTSSTDPFSASSNSSNTSVETWKHNDPFAPGGTVVAAASDSATDPFASVFGNESFGDGFADFSTLSK VNNEDAFNPTISSSTSSVTIAKPMLEETASKSEDVPPALPPKVGTPTRPCPPPPGKRPINKLDSSDPLKL NDPFQPFPGNDSPKEKDPDMFCDPFTSSTTTNKEADPSNFANFSAYPSEEDMIEWAKRESEREEEQRLAR LNQQEQEDLELAIALSKSEISEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001159964 |
| ORF Size | 1749 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001159964.1, NP_001153436.1 |
| RefSeq Size | 4244 bp |
| RefSeq ORF | 1752 bp |
| Locus ID | 13858 |
| UniProt ID | P42567 |
| MW | 64.8 kDa |
| Gene Summary | Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219270 | Eps15 (untagged) - Mouse epidermal growth factor receptor pathway substrate 15 (Eps15), transcript variant 2, (10ug) |
CNY 7,410.00 |
|
| MG226696 | Eps15 (tGFP-tagged) - Mouse epidermal growth factor receptor pathway substrate 15 (Eps15) transcript variant 2, (10ug) |
CNY 8,112.00 |
|
| MR226696L3 | Lenti ORF clone of Eps15 (Myc-DDK-tagged) - Mouse epidermal growth factor receptor pathway substrate 15 (Eps15), transcript variant 2 |
CNY 7,700.00 |
|
| MR226696L4 | Lenti ORF clone of Eps15 (mGFP-tagged) - Mouse epidermal growth factor receptor pathway substrate 15 (Eps15), transcript variant 2 |
CNY 7,700.00 |
