Gp1bb (NM_010327) Mouse Tagged ORF Clone

CAT#: MR226871

  • TrueORF®

Gp1bb (Myc-DDK-tagged) - Mouse glycoprotein Ib, beta polypeptide (Gp1bb), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_010327" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,090.00


货期*
2周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR226871 representing NM_010327
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTCCCGGCCCCGTGGGGCGCTGAGCCTGCTGCTTCTGTTGCTAGCGCTGCTCAGCCGCCCAGCCT
CTGGCTGCCCCGCGCCATGCAGCTGCGCAGGGACGCTCGTGGACTGTGGGCGCCGCGGGCTGACCTGGGC
GTCGCTGCCGGCTGCCTTCCCTCCCGACACCACCGAATTGGTGCTGACCGGCAACAACCTGACGGCGCTG
CCGCCCGGGCTTCTGGACGCGTTGCCTGCTCTGCGCGCAGCACACCTGGGCGCCAACCCCTGGCGCTGCG
ACTGCCGCCTATTGCCGCTGCGCGCCTGGCTGGCCGGCCGCCCGGAGCGCGCGCCCTACCGCGACCTGCG
CTGTGTCGCGCCCCCAGCGCTGCGCGGCCGCTTGCTGCCCTACGTGGCGGAGGATGAGCTGCGGGCCGCG
TGCGCACCCGGTCTACTCTGCTGGGGAGCACTGGTGGCCCAGCTAGCACTGCTGGTCCTCGGGCTGCTAC
ACGCGCTGCTATTGGCACTTCTACTAGGTCGCCTGCGGAGGCTGCGCGCGCGTGCGCGCGCACGCTCCAT
CCAAGAGTTCTCTCTCACAGCCCCGCTGGTGGCCGAGTCGGCCAGAGGTGGTGCATCC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>MR226871 representing NM_010327
Red=Cloning site Green=Tags(s)

MGSRPRGALSLLLLLLALLSRPASGCPAPCSCAGTLVDCGRRGLTWASLPAAFPPDTTELVLTGNNLTAL
PPGLLDALPALRAAHLGANPWRCDCRLLPLRAWLAGRPERAPYRDLRCVAPPALRGRLLPYVAEDELRAA
CAPGLLCWGALVAQLALLVLGLLHALLLALLLGRLRRLRARARARSIQEFSLTAPLVAESARGGAS

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_010327
ORF Size 618 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_010327.2, NP_034457.1
RefSeq Size 1847 bp
RefSeq ORF 621 bp
Locus ID 14724
UniProt ID P56400
MW 22.2 kDa
Gene Summary Gp-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to von Willebrand factor, which is already bound to the subendothelium.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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