Tchh (NM_001163098) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AHF; AI597080; Thh
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR227079 representing NM_001163098, codon optimized. Due to the complexity of NM_001163098, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCCGTTGATCAGATCTATTGTGGATATCACAGAGGTGTTTAATCAGTATGCTAGCCAGTCCTGTG ACGGGGCCTCACTGAGCAAGAAGGATTTGAAGAATTTGCTTGAGAGAGAACTTGGAGATGTGCTGCAGAG GCCCCACGATCCCGAAACAATTGATCTGACTCTGGAGCTGCTTGACCGAGACTGCAACGGTAGAGTGGAT TTCAACGAGTTCCTCCTTTTCCTGTTTAAGATAGCCCAGGCATGCTACTACGCCCTGGACCAAGCAGCTG AGCTGGGTGAGAAGCGGGCTCTGCCTAACGAGAAGCGAAACCTTAGTCAGGACCGGCGGCAGGAGGACCA GCGGAGGTTTGAACCTAGGAGCCGACAGCTCGACGAGGAACCAGGGAGAAGATCCTGGCAGAAGAGAAGA GAGCAGGAAGAGCGAGCAGAGGAGCAGAGATTGGAACAGAGGTATCGGCAGCACCGGGACGAGGAACAGA GGCTGCAGCGCCGGGAGCTGCAGGAATTGGAAGAGCGCCTTGCCGAAAAGGAGCCCCTTGGCTGGTCTAA GGGACGCGATGCTGAGGAGTTTAGCGAGGTCGAGGAGCAGCAGAGGCAAGAACGGCAGGAACTTAAAGGA AAGGGACAGACAGAGGAGCGAAGACTGCAGAAACGCCGGCAGGAGGAACTCAGAGAGCCATTGCTTCGCA GAGATCTTGAACTGAGACGCGAGCAGGAACTTAGGCGAGAGCAGGAGCTTAGGCAGGAACAACGCCGCGA ACAGGAGCTTAGGCGGGAGCAGGAACTGCGGCAGGAACTGAGGAGGGAACAGGAGCTGAATAGGAGACAG GAGCTCAGGCGCGAACAAGAGCTTAGACGAGAGCAGGAACTGCGGCAAGAGTTGAGGCGGGAGCAAGAAC TCCGGAGAGAGCAGGAACTCCGGCAGGAACTGCGACGCGAGCAAGAGCTCAGGCGCGAACAAGAGCTTCG CCAGGAGCTGCGAAGAGAACAGGAGCTGCGAAGAGAACAGGAGCTGCGGCAGGAGTTGCGGCGGGAGCAG GAGCTGAGACGCGAGCAGGAACTTAGGCAGGAACTGGCGGAAGAGGATGAACTGACTAGGATCCGCGAGC CAGACGAATCTATTACACAGCGGTGGCAGTGGCAGCTGGAGAATGAGGCAGATGCAAGGCAGAATAAGGT TTACTCCAGACCCAGCCGGCAGGAACAACGCTTGAGGCAGGAACTTGGAGAGCGCCAGCTGCGCGAACAG GAGGAACAGCGGCGCGATCTGCAGCAGGAGCGGCCTGCTGAGGAAGCGAGGCAGCGAAACCAGTGGGAAA GACCTCAGAGGGCAGAGGAAAGGCTCGAACAGGAACAGAGGTTCAGAGACAGGGAAGAGCAGAGGTTCCG GGAGGAGAAGCTGCAACGAGCCGAGCTCCAGGATTCACTCCTTGATGAAGAACAAAGACGCCTGCAGGAG GAGCGAAGAGAACCTAACCGAAGTCGCCAGCTTAGAGAGGAGTCCCAGCGCAGGCGCACACTTTACGCAA AACCAAGTCAGCGCCAAAGAAGGGAGGAAGAACTGCGGGAGGAACGCCTCTTGCAGGAAGAGCAGCGACA GCAGAGGGAAAGGAAGCACAGAAGGGAAGAGGATCTGCAGCAGGAGGAAAAGCGACTGCAGCAAGATGAG GAGCAGTTGCAGAGGGAAAGACGGAGACTCCAGAGGGAAAGACAGTATCAGGAAGAGGATCTCCAGCAGG AAGAGGAAAGGCTGCAGCAAGAGGAGGAAAGGCTGCAGCGGGAACGAAGGAGGCTGCAGCAGGAACGACA ATACCAGGAAGAGGACTTGCAACGCCTGCGGGACGAGGACCAAAGAAGGGACCTGAAGTGGCAGTGGCAG CCTCGAAAGGAAAACGAGGTAAGATCTAACAGGCTCTTTACCAAAAGGCGAGGAGACGAAGAGCCTATCC AGCAACTGGAGGATTCACAGGAGCGAGAAAGAAGACAAGACCGCCGGCCCCTTCAGGACGAGGAGGAAGA GAAACGCGAGCTCGAGCAGGAGCGCCGCCGACGGCAGCAAAGGGACCGGCAGATCCTCGAAGAGGAGCAA TTTCAGAGGGAGCACCAGCGCGAGGCTCGGAGACGAGATGAAACATTCCAAGAGGAAGAGCAACTCCAGG GGGAAAGCCGCAGGAGACAACAGGAAAGAGAGGGCAAATTTCTGGAGGAAGAAAGACAGTTGCGAACAGA GCGGGAAGAGCAGAGGCGCCGCCAAGAGCAAGAGCGCGAGTTCCAAGAAGAAGAGGAACATCTGCAGGAA AGGGAGAAGGAGCTCCGCCAGGAATGTGATCGGAAAAGTCGAGAACAGGAGAGGCGGCAGCAGAGAGAGG AAGAGCAGTTGAGACGGCAAGAACGGGACCAGCGATTCCGGCGCGAACAAGAGCGGCATCTGGAGAGAGA GGAAGAGCAGCTCCGGGACCGCCCATCCAGGCGCGAGCAGGAGCGACACCAGGAGAGGGAAGAAGAACAA CTCAGAGATAGACCCTCCCGCCGGGAACAGGAGAGACATCAGGAGAGAGAAGAGGAACAGCTCCGCGATA GGCCATCAAGACGGGAGCAGGAGCGGCACCAGGAACGAGAAGAAGAGCAGCTCCGGGATCGCCCCTTTCG AAGGGAGCAAGAGAGACGACTGGAGAGAGAGGAGGAACAGCTCCGAGACAGACCTAGCCGGCGCGAACAA GAGAGGCACCAGGAGCGGGAGGAAGAGCAACTCAGGGACCGCCCTAGCCGACGGGAGCAGGAGCGCCGAT TGGAGAGAGAAGAAGAACAACTCCGCGATAGAAGTTTCAGACGGGAGCAGGAGCTGAGACGGGATAGAAA ATTCCACGAGGAAGAGGAGCGGAGAGAAGAGCTGGAAGAGGAGCAGCGAGGGCAGGAGCGGGACCGCCTG AGGGTCGAGGAACAATTGCGCGGGCAGCGAGAGGAAGAGCAGCGCAGACGGCAGGAATGCGACAGAAAAC TGCATCGGGAACTGGAGGTAAGACAGGAATTGGAAGAGGAGCGCCTGAGGGATCGCAAGCTCCGAAGAGA GCAGGAACTGAGACGCGACAGAAAATTTCATGAGGAGGAAGAGCGGAGGCATGAGGAGTTCGAGGAGAAG CAGCTTAGACTCCAGGAGCCAGACAGGAGGTTCCGGCGGGAGCAGGAACTCCGCCAGGAATGCGTGGAGG AGGAGAGACTCCGGGACTCTAAGATCCGCCGAGAGCAGGAACTGAGACGGGAAAGAGAAGAGGAGAGGCT CCGGGATAGAAAGATTAGACGCGATCAGGAATTGAGACAGGGGCTTGAGGAGGAGCAGCTCCGAAGACAA GAACTCGACAGGAAATTCCGCGAAGAACAGGAGCTTGACCAGGAATTGGAGGAAGAGCGGCTCCGCGACC GAAAGATTCGCCGCGAGCAAGAGCTGCGAAGAGAGCAGGAACTCCGCAGGGAACAAGAATTTCGGAGGGA GCAGGAGCTGCGCCGGGAACAAGAGTTCCGCCGGGAGCAAGAGCTCCGCCAGGAAAGAGAGGAAGAGAGG CTCCGAGACAGAAAAATAAGACGGGATCAGGAGCTGCGGCAAGGGCTGGAAGAGGAGCAGTTGAGGAGGC AAGAGAGGGACAGGAAGGTCCGCGAGGAACAAGAGCTGGACCAGGAGCTTGAGGAAGAGCGACTTAGGGA CCGCAAGATTAGACGAGAACAGGAGCTGCGCAGGGAGCAAGAGCTGCGCCGAGAGCAGGAATTTCGCAGA GAGCAGGGTTTGAGGCGCGAAAGAGAGGAGGAAAGGCTCCGCGACCGGAAGATAAGAAGAGATCAGGAGC TGAGACAGGGACTGGAAGAAGAGCAACTGAGAAGGCAGGAACGGGACCGGAAGTTTAGAGAGGAACAGGA ACTCGGACAGGAGCTGGAAGAGGAAAGACTCCGCGATAGGAAGATCCGGCGCGAACAGGAACTGCGCCGG GAACGGGAACAGGAGCAGCGACGCCGGCTGGAAAGGGAGGAAGAACAGCAGCGACTGCACGAGCGCGAAG AGGAACAGAGACGCAGACAGGAAAGAGAGCAAGAACAGCAGCGATGTCTGGAAAGGGAGGAAGAACAATT CAGGTTCGAGGAACAGCAGAGGAGACGGCAGGAGCGAGAGCAGCAGTTGAGGCAAGAGCGAGATAGAAGG GTACTTGAAGAGGAGGAGCTCCGGCAAGAGCGCGAAGAGCTGCTCCATCGACAGGTCGGTGGACGCAAAT TTCGGGAAGAAGAAAGGCTGCGGCTGGAAAGGGAAGAACAGCAAAGAAGGCTCCAAGAAAGGGACAATCG CCGGTTCAGAGAGGAGGTGGAGCTTCGCCAAGAGAGGGAAGGCCAGCAACTCCGCCAGGAGAGAGATCGC AAGTTTAGGGAAGTTGAAGAGCTGCGCCAGGAAGAACAGAGGAGACGACAGGAGCGGGATCGCAAGTTCA GGGAGGAAAAACACCCACGGGAAGAGCGCGAGGAACAGCAGCTGAGGCGGGAGAAGCGGGACGGACAGTA CCTCGCAGAAGAGCAGTTCGCGAGAGATACCATTCGCAGACAGGAACAAGAGCTGCGGCAAGAAGAGGAG CAGCGCCGCCGACAGGAGAGGGAGAGAAAATTCCAGGAGGAGCAGATCCGGCGGAGGCAGGAAGAGCAGA GGAGGCGACAGATACTGGAACCTGGTACACGGCAGTTTGCAAATGTTCCCGTCAGGAGCTCTCCTCTCTA CGAGTACATTCAGGAGCAGAGATCTCAGTATAGGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR227079 representing NM_001163098 Red=Cloning site Green=Tags(s) MSPLIRSIVDITEVFNQYASQSCDGASLSKKDLKNLLERELGDVLQRPHDPETIDLTLELLDRDCNGRVD FNEFLLFLFKIAQACYYALDQAAELGEKRALPNEKRNLSQDRRQEDQRRFEPRSRQLDEEPGRRSWQKRR EQEERAEEQRLEQRYRQHRDEEQRLQRRELQELEERLAEKEPLGWSKGRDAEEFSEVEEQQRQERQELKG KGQTEERRLQKRRQEELREPLLRRDLELRREQELRREQELRQEQRREQELRREQELRQELRREQELNRRQ ELRREQELRREQELRQELRREQELRREQELRQELRREQELRREQELRQELRREQELRREQELRQELRREQ ELRREQELRQELAEEDELTRIREPDESITQRWQWQLENEADARQNKVYSRPSRQEQRLRQELGERQLREQ EEQRRDLQQERPAEEARQRNQWERPQRAEERLEQEQRFRDREEQRFREEKLQRAELQDSLLDEEQRRLQE ERREPNRSRQLREESQRRRTLYAKPSQRQRREEELREERLLQEEQRQQRERKHRREEDLQQEEKRLQQDE EQLQRERRRLQRERQYQEEDLQQEEERLQQEEERLQRERRRLQQERQYQEEDLQRLRDEDQRRDLKWQWQ PRKENEVRSNRLFTKRRGDEEPIQQLEDSQERERRQDRRPLQDEEEEKRELEQERRRRQQRDRQILEEEQ FQREHQREARRRDETFQEEEQLQGESRRRQQEREGKFLEEERQLRTEREEQRRRQEQEREFQEEEEHLQE REKELRQECDRKSREQERRQQREEEQLRRQERDQRFRREQERHLEREEEQLRDRPSRREQERHQEREEEQ LRDRPSRREQERHQEREEEQLRDRPSRREQERHQEREEEQLRDRPFRREQERRLEREEEQLRDRPSRREQ ERHQEREEEQLRDRPSRREQERRLEREEEQLRDRSFRREQELRRDRKFHEEEERREELEEEQRGQERDRL RVEEQLRGQREEEQRRRQECDRKLHRELEVRQELEEERLRDRKLRREQELRRDRKFHEEEERRHEEFEEK QLRLQEPDRRFRREQELRQECVEEERLRDSKIRREQELRREREEERLRDRKIRRDQELRQGLEEEQLRRQ ELDRKFREEQELDQELEEERLRDRKIRREQELRREQELRREQEFRREQELRREQEFRREQELRQEREEER LRDRKIRRDQELRQGLEEEQLRRQERDRKVREEQELDQELEEERLRDRKIRREQELRREQELRREQEFRR EQGLRREREEERLRDRKIRRDQELRQGLEEEQLRRQERDRKFREEQELGQELEEERLRDRKIRREQELRR EREQEQRRRLEREEEQQRLHEREEEQRRRQEREQEQQRCLEREEEQFRFEEQQRRRQEREQQLRQERDRR VLEEEELRQEREELLHRQVGGRKFREEERLRLEREEQQRRLQERDNRRFREEVELRQEREGQQLRQERDR KFREVEELRQEEQRRRQERDRKFREEKHPREEREEQQLRREKRDGQYLAEEQFARDTIRRQEQELRQEEE QRRRQERERKFQEEQIRRRQEEQRRRQILEPGTRQFANVPVRSSPLYEYIQEQRSQYRP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001163098
ORF Size	4797 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001163098.1, NP_001156570.1
RefSeq Size	5823 bp
RefSeq ORF	4800 bp
Locus ID	99681
MW	208.2 kDa

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