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Ttbk1 (NM_001162864) Mouse Tagged ORF Clone
CAT＃: MR227113
- TrueORF®
Ttbk1 (Myc-DDK-tagged) - Mouse tau tubulin kinase 1 (Ttbk1). Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_001162864" in other vectors (4)
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CNY 10,016.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AU017937; AW048023; C330008L01Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227113 representing NM_001162864, codon optimized.
Due to the complexity of NM_001162864, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAATGCCTTGCAGCTGCTCTGAAGGACGAGACTAACATGTCAGGCGGTGGGGAGCAAGCGGACATTC TGCCTGCGAACTATGTGGTTAAAGACCGGTGGAAGGTGCTGAAAAAGATCGGAGGAGGCGGATTCGGGGA GATCTACGAGGCAATGGATCTGTTGACACGAGAGAATGTAGCCCTTAAGGTGGAGAGTGCCCAGCAGCCG AAGCAGGTCCTGAAGATGGAGGTGGCCGTCCTTAAGAAGCTGCAGGGCAAAGACCACGTTTGTCGATTCA TCGGCTGCGGGAGAAATGAAAAATTTAATTACGTGGTCATGCAGCTCCAGGGACGGAACCTCGCCGATCT TCGAAGATCTCAGCCACGGGGCACATTTACGCTGTCCACAACTTTGCGGCTGGGCAAGCAGATCCTTGAG TCTATTGAGGCCATTCACAGCGTGGGATTCCTTCACAGAGATATCAAGCCGTCTAACTTTGCAATGGGGC GCCTCCCTTCTACATACCGGAAATGTTACATGCTTGATTTTGGCCTTGCCAGGCAGTACACCAATACCAC CGGGGACGTGCGGCCACCCAGGAACGTGGCAGGATTCCGCGGGACAGTGCGGTATGCCTCTGTTAATGCC CACAAAAATCGGGAGATGGGGCGCCATGATGACCTGTGGAGCCTGTTCTATATGTTGGTGGAATTCGCGG TGGGACAGCTGCCATGGCGCAAGATCAAGGACAAGGAGCAGGTGGGCATGATCAAAGAGAAGTATGAGCA TAGGATGTTGCTCAAACACATGCCCAGCGAGTTCCATCTGTTCCTTGACCACATCGCAAGCCTCGACTAC TTTACAAAGCCTGATTACCAGTTGATTATGTCAGTTTTCGAGAACAGCATGAAGGAACGAGGAATCGCAG AGAATGAGGCTTTCGACTGGGAGAAGGCCGGCACAGACGCTCTCCTGTCAACAAGCACCTCTACCCCCCC TCAGCAGAACACTAGGCAGACTGCTGCCATGTTTGGAGTGGTCAATGTTACACCCGTCCCAGGTGACCTT CTCAGGGAGAACACTGAAGATGTCCTTCAGGGGGAGCATTTGTCTGATCAGGAAAACGCTCCTCCGATTC TCCCCGGAAGACCGCCCGAAGGCCTGGGCCCTGGACCCCATCTTGTGCCTCATCCAGGAGGTCCTGAGGC CGAGGTGTGGGAGGAAACCGATGTGAATCGGAACAAGCTTCGAATCAACATCGGAAAAACTCCATGTGTC GAGGAGGAGCAGAGCAGAGGAGTTGGGGTACCCTCCAGTCCAGTGCGAGCACCTCCGGACAGCCCTACAA CGCCTGTGAGGTCCCTTTGCTATAGGAGAGTGAACAGCCCAGAAAGCGAGCGGCTTTCCACCGCTGCAGA CGGTCGGGTTGAGCTTCAGGAACGGCGATCCAGGATGGACCTGCCTGGTTCACCATCTAGGCAAGCATGT TCCTCACAGCCAGCTCAGATGCTTTCCGTGGACACCGGACATGCTGATAGACAAGCCAGCGGCCGGATGG ATGTAAGTGCCAGTGTCGAGCAAGAAGCTCTGTCCAACGCATTCAGGTCTGTGCCTCTGGCAGAGGAGGA GGACTTCGATTCTAAAGAGTGGGTCATAATCGACAAGGAAACCGAATTGAAGGATTTCCCCCCCGGCGCT GAACCGAGCACTTCCGGCACCACCGACGAGGAACCGGAAGAGCTGCGCCCCTTGCCCGAGGAGGGCGAGG AAAGGCGCCGACTGGGAACAGAACCTACAGTTCGCCCTCGCGGCCGCGGGATGCACACGCTTACTGAGGA GGATCCTAGGCAAATGCTCCCCCAGCCCGCTCCACCACAGCTGTCCCAGGCTGACGGCCGCAGCGAGACA TCTCAACCCCCCACTCCCGGTTCCCCCAGCCACTCCCCCCTGCACTCAGGACCTAGGCCCCGCCGGCGAG AATCAGATCCGACAGGACCCCAGAGGCAGGTGTTTAGTGTGGCCCCGCCTTTCGAGGTGAATGGACTGCC TAGGGCCGTGCCTCTCGCTCTTCCATACCAGGACTTTAAGAGGGACCTGTCTGACTACAGGGAACGAGCA CGCCTGCTGAATCGCGTACGGCGAGTGGGCTTCAGTCACATGCTGCTGACCGCCCCTCAAGTCCCTCTCG CGCCATTCCAACCACAGGCGAATGGTAAAGAGGGCGAGGAGGAGGAGGAAGAAGAGGAAGAGGAAGAGGA GGAAGAGGAGGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAGGAAGAAGAGGAGGAAGAGGAGGCAGGA GCATTGGGTGAGGTTCTTGGGCCCAGAAGCGGTTCTTCCAGCGAGGGCTCCGAGAGGTCAACCGAGCGCA GCCAGGAAGGAGCACCTAGTACTCTGCTTGCCGACGATCAGAAGGAGGCAAGGGGGCGCGCCTCTATGGC CGACGGCGATCTGGAACCAGAGGAGGGTAGCAAAACGCTTGTGCTTGTCAGCCCCGGCGATATGAAGAAG AGTCCAGTTACCGCTGAGCTGGCACCCGATCCAGACTTGGGCACCTTGGCTGCTCTTACCCCCCAGCACG AGAGGCCCCAGCCCACCGGTTCCCAATTGGACGTTTCTGAGCCAGGAACACTGAGCTCTATTCTCAAATC CGAACCAAAGCCGAGCGGCCCAGGGGCTGGGGGAGGAGTGGGCCTGGTTGCGCCCGGGGCCGGGGTCACC GCTGTGACCTCCCCTTTTACTAAGGTTGAGCGCACCTTCGTGCACATCGCTGAGAAGTCACACCTCAATG TGATGAGTTCCGGCGGGCAGGCCTCTCGGCCAGAGGAGCTTTCCACCGGGGGCGAGCTCGGACTTGAGGT GCTTTCTGAAGGGGGGATCGCAGAGGAGGGTGCTCCTGCTCCACTTGAGAATGGAATGGCTCTGGCTGGG CTGGATGGAACAGAGATGGAAAGTTGCGCTCTCTCTGGACCACCAGGAGAGACACCTTCTGAAGTGGTGA CGGATAGCCTCCCAAACGGGCCAGCCCTGGCCGACGGACCTGCTCCCGCCAGCCAACAGGAGCCGGTGAC CAAAAAAGGTACCACCATATCACCTTCACGCCACGCCATGCCCGGTAGCCGCCCCCGGTCCAGAATTCCA GTGCTGCTTTCAGAGGAAGATACCGGTTCTGAGCCCAGCGGGAGCCTGAGTGCCAAGGAGAGATGGAGTA AGAGAGCACGCCCACAGCAGGACTTGGCCAGGCTGGTCATGGAGAAACGGCAGGGCAGGCTCCTCCTGAG ACTGGCTAGCGGCGCCTCTTCAAGCAGCAGTGAGGAACAGCGGAGGGCCAGCGAAACCCTGAGTGGCACC GGCAGCGAGGAGGACACCCCCGCCTCTGAGCCTACTACTGCTCTCCCACGCAAGGCCGTGCGCGCTGCCA CGACCCGGTCACGCATCCCGCGGCCCATCAGCGTATCTATGCCTGTTGAGGGCCAACAGCTCCCAGGCAG GCCACACGGAGCTGCATCAGCAACGGATCTCGCAATCACGAGTAGGCTGCAGTTGCAGAAGCCATCTGGG CTGGCGCCAGCTGCTGATCTCCGGCCTAAACAGTCCGCTTCCAGGGGCCCGGGCCCTGGCCGCGCCCAAG TGTCTAAGCCTGCCGCCCCGAGATCACCCGGCCTGCCAGCATCTACTGCAAGGCATCCCTCCGGATCACC GAGGAGCCAGAGCCTTAGCAGGAAAGAGTCTAGTAGCCCCTCCCACCAGGCCCGGCCTGGGGTGCCCCCA TCTCGCGGCGTCCTCCAGGTGCGGTCCCAACCAGAGGCAAGTCCTGTGGCCCCTAAGAAGGGGCCTAAGG GAAAGCAGCTGCAGACTCAGCGGGCCGCCACAAAGGGCCGGGCCGTAGTATCTGAGGGCAGGCCTGGGGC AAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227113 representing NM_001162864
Red=Cloning site Green=Tags(s) MQCLAAALKDETNMSGGGEQADILPANYVVKDRWKVLKKIGGGGFGEIYEAMDLLTRENVALKVESAQQP KQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKFNYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILE SIEAIHSVGFLHRDIKPSNFAMGRLPSTYRKCYMLDFGLARQYTNTTGDVRPPRNVAGFRGTVRYASVNA HKNREMGRHDDLWSLFYMLVEFAVGQLPWRKIKDKEQVGMIKEKYEHRMLLKHMPSEFHLFLDHIASLDY FTKPDYQLIMSVFENSMKERGIAENEAFDWEKAGTDALLSTSTSTPPQQNTRQTAAMFGVVNVTPVPGDL LRENTEDVLQGEHLSDQENAPPILPGRPPEGLGPGPHLVPHPGGPEAEVWEETDVNRNKLRINIGKTPCV EEEQSRGVGVPSSPVRAPPDSPTTPVRSLCYRRVNSPESERLSTAADGRVELQERRSRMDLPGSPSRQAC SSQPAQMLSVDTGHADRQASGRMDVSASVEQEALSNAFRSVPLAEEEDFDSKEWVIIDKETELKDFPPGA EPSTSGTTDEEPEELRPLPEEGEERRRLGTEPTVRPRGRGMHTLTEEDPRQMLPQPAPPQLSQADGRSET SQPPTPGSPSHSPLHSGPRPRRRESDPTGPQRQVFSVAPPFEVNGLPRAVPLALPYQDFKRDLSDYRERA RLLNRVRRVGFSHMLLTAPQVPLAPFQPQANGKEGEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAG ALGEVLGPRSGSSSEGSERSTERSQEGAPSTLLADDQKEARGRASMADGDLEPEEGSKTLVLVSPGDMKK SPVTAELAPDPDLGTLAALTPQHERPQPTGSQLDVSEPGTLSSILKSEPKPSGPGAGGGVGLVAPGAGVT AVTSPFTKVERTFVHIAEKSHLNVMSSGGQASRPEELSTGGELGLEVLSEGGIAEEGAPAPLENGMALAG LDGTEMESCALSGPPGETPSEVVTDSLPNGPALADGPAPASQQEPVTKKGTTISPSRHAMPGSRPRSRIP VLLSEEDTGSEPSGSLSAKERWSKRARPQQDLARLVMEKRQGRLLLRLASGASSSSSEEQRRASETLSGT GSEEDTPASEPTTALPRKAVRAATTRSRIPRPISVSMPVEGQQLPGRPHGAASATDLAITSRLQLQKPSG LAPAADLRPKQSASRGPGPGRAQVSKPAAPRSPGLPASTARHPSGSPRSQSLSRKESSSPSHQARPGVPP SRGVLQVRSQPEASPVAPKKGPKGKQLQTQRAATKGRAVVSEGRPGAR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001162864 |
| ORF Size | 3924 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001162864.1, NP_001156336.1 |
| RefSeq Size | 6959 bp |
| RefSeq ORF | 3927 bp |
| Locus ID | 106763 |
| UniProt ID | Q6PCN3 |
| MW | 141.6 kDa |
| Gene Summary | Serine/threonine kinase which is able to phosphorylate TAU on serine, threonine and tyrosine residues. Induces aggregation of TAU (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224207 | Ttbk1 (untagged) - Mouse tau tubulin kinase 1 (Ttbk1), (10ug) |
CNY 16,820.00 |
|
| MG227113 | Ttbk1 (tGFP-tagged) - Mouse tau tubulin kinase 1 (Ttbk1), (10ug). Note: ORF is codon optimized |
CNY 12,640.00 |
|
| MR227113L3 | Lenti ORF clone of Ttbk1 (Myc-DDK-tagged) - Mouse tau tubulin kinase 1 (Ttbk1). Note: ORF is codon optimized |
CNY 13,400.00 |
|
| MR227113L4 | Lenti ORF clone of Ttbk1 (mGFP-tagged) - Mouse tau tubulin kinase 1 (Ttbk1). Note: ORF is codon optimized |
CNY 13,400.00 |
