Stat3 (NM_213660) Mouse Tagged ORF Clone
CAT#: MR227269
- TrueORF®
Stat3 (Myc-DDK-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 2
ORF Plasmid: tGFP
"NM_213660" in other vectors (4)
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CNY 7,700.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1110034C02Rik; A; Aprf; AW109958 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR227269 representing NM_213660
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGTGGAACCAGCTGCAGCAGCTGGACACACGCTACCTGGAGCAGCTGCACCAGCTGTACAGCG ACAGCTTCCCCATGGAGCTGCGGCAGTTCCTGGCACCTTGGATTGAGAGTCAAGACTGGGCATATGCAGC CAGCAAAGAGTCACATGCCACGTTGGTGTTTCATAATCTCTTGGGTGAAATTGACCAGCAATATAGCCGA TTCCTGCAAGAGTCCAATGTCCTCTATCAGCACAACCTTCGAAGAATCAAGCAGTTTCTGCAGAGCAGGT ATCTTGAGAAGCCAATGGAAATTGCCCGGATCGTGGCCCGATGCCTGTGGGAAGAGTCTCGCCTCCTCCA GACGGCAGCCACGGCAGCCCAGCAAGGGGGCCAGGCCAACCACCCAACAGCCGCCGTAGTGACAGAGAAG CAGCAGATGTTGGAGCAGCATCTTCAGGATGTCCGGAAGCGAGTGCAGGATCTAGAACAGAAAATGAAGG TGGTGGAGAACCTCCAGGACGACTTTGATTTCAACTACAAAACCCTCAAGAGCCAAGGAGACATGCAGGA TCTGAATGGAAACAACCAGTCTGTGACCAGACAGAAGATGCAGCAGCTGGAACAGATGCTCACAGCCCTG GACCAGATGCGGAGAAGCATTGTGAGTGAGCTGGCGGGGCTCTTGTCAGCAATGGAGTACGTGCAGAAGA CACTGACTGATGAAGAGCTGGCTGACTGGAAGAGGCGGCAGCAGATCGCGTGCATCGGAGGCCCTCCCAA CATCTGCCTGGACCGTCTGGAAAACTGGATAACTTCATTAGCAGAATCTCAACTTCAGACCCGCCAACAA ATTAAGAAACTGGAGGAGCTGCAGCAGAAAGTGTCCTACAAGGGCGACCCTATCGTGCAGCACCGGCCCA TGCTGGAGGAGAGGATCGTGGAGCTGTTCAGAAACTTAATGAAGAGTGCCTTCGTGGTGGAGCGGCAGCC CTGCATGCCCATGCACCCGGACCGGCCCTTAGTCATCAAGACTGGTGTCCAGTTTACCACGAAAGTCAGG TTGCTGGTCAAATTTCCTGAGTTGAATTATCAGCTTAAAATTAAAGTGTGCATTGATAAAGACTCTGGGG ATGTTGCTGCCCTCAGAGGGTCTCGGAAATTTAACATTCTGGGCACGAACACAAAAGTGATGAACATGGA GGAGTCTAACAACGGCAGCCTGTCTGCAGAGTTCAAGCACCTGACCCTTAGGGAGCAGAGATGTGGGAAT GGAGGCCGTGCCAATTGTGATGCCTCCTTGATCGTGACTGAGGAGCTGCACCTGATCACCTTCGAGACTG AGGTGTACCACCAAGGCCTCAAGATTGACCTAGAGACCCACTCCTTGCCAGTTGTGGTGATCTCCAACAT CTGTCAGATGCCAAATGCTTGGGCATCAATCCTGTGGTATAACATGCTGACCAATAACCCCAAGAACGTG AACTTCTTCACTAAGCCGCCAATTGGAACCTGGGACCAAGTGGCCGAGGTGCTCAGCTGGCAGTTCTCGT CCACCACCAAGCGGGGGCTGAGCATCGAGCAGCTGACAACGCTGGCTGAGAAGCTCCTAGGGCCTGGTGT GAACTACTCAGGGTGTCAGATCACATGGGCTAAATTCTGCAAAGAAAACATGGCTGGCAAGGGCTTCTCC TTCTGGGTCTGGCTAGACAATATCATCGACCTTGTGAAAAAGTATATCTTGGCCCTTTGGAATGAAGGGT ACATCATGGGTTTCATCAGCAAGGAGCGGGAGCGGGCCATCCTAAGCACAAAGCCCCCGGGCACCTTCCT ACTGCGCTTCAGCGAGAGCAGCAAAGAAGGAGGGGTCACTTTCACTTGGGTGGAAAAGGACATCAGTGGC AAGACCCAGATCCAGTCTGTAGAGCCATACACCAAGCAGCAGCTGAACAACATGTCATTTGCTGAAATCA TCATGGGCTATAAGATCATGGATGCGACCAACATCCTGGTGTCTCCACTTGTCTACCTCTACCCCGACAT TCCCAAGGAGGAGGCATTTGGAAAGTACTGTAGGCCCGAGAGCCAGGAGCACCCCGAAGCCGACCCAGGT AGTGCTGCCCCGTACCTGAAGACCAAGTTCATCTGTGTGACACCAACGACCTGCAGCAATACCATTGACC TGCCGATGTCCCCCCGCACTTTAGATTCATTGATGCAGTTTGGAAATAACGGTGAAGGTGCTGAGCCCTC AGCAGGAGGGCAGTTTGAGTCGCTCACGTTTGACATGGATCTGACCTCGGAGTGTGCTACCTCCCCCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227269 representing NM_213660
Red=Cloning site Green=Tags(s) MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSR FLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEK QQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTAL DQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQ IKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVR LLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGN GGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNV NFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFS FWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISG KTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPG SAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMDLTSECATSPM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_213660 |
ORF Size | 2307 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_213660.1, NM_213660.2, NM_213660.3, NP_998825.1 |
RefSeq Size | 4484 bp |
RefSeq ORF | 2310 bp |
Locus ID | 20848 |
UniProt ID | P42227 |
MW | 88.4 kDa |
Gene Summary | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221434 | Stat3 (untagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 2, (10ug) |
CNY 9,880.00 |
|
MG227269 | Stat3 (tGFP-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3) transcript variant 2, (10ug) |
CNY 8,460.00 |
|
MR227269L3 | Lenti ORF clone of Stat3 (Myc-DDK-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 2 |
CNY 9,600.00 |
|
MR227269L4 | Lenti ORF clone of Stat3 (mGFP-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 2 |
CNY 9,600.00 |